一次抗体と二次抗体-抗-エピトープタグ、免疫測定、Cas 9 » エピトープタグ抗体ファミリー » THE™ DYKDDDDK Tag Antibody [iFluor 488], mAb, Mouse
THE™ DYKDDDDK Tag Antibody [iFluor 488], MAb, Mouse

Flow cytometric analysis of non-transfected CHO cells (Black) or Flag-tagged protein transfected CHO cells (Red)
using THETM DYKDDDDK Tag Antibody [iFluor 488], mAb, Mouse (A01809-100, 4 µg/ml).

THE™ DYKDDDDK Tag Antibody [iFluor 488], MAb, Mouse

Immunocytochemistry/Immunofluorescence analysis of non-transfected CHO cells (a) or Flag-tagged protein transfected CHO cells (b)
using THETM DYKDDDDK Tag Antibody [iFluor 488], mAb, Mouse (A01809-100, 4 µg/ml).

THE™ DYKDDDDK Tag Antibody [iFluor 488], mAb, Mouse

THE™ DYKDDDDK Tag Antibody [iFluor 488], mAb, Mouse recognizes N-terminal, internal and C-terminal Flag-tagged proteins.
A01809
¥31,091.00

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Overview
Specificity THE™ DYKDDDDK Tag Antibody [iFluor 488], mAb, Mouse recognizes N-terminal, internal and C-terminal Flag-tagged proteins.
Host Species Mouse
Immunogen A synthetic peptide DYKDDDDK coupled to KLH
Conjugate iFluor 488

Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Immunocytochemistry/Immunofluorescence (ICC/IF)  1-4 µg/ml
Flow Cytometry  1-4 µg/ml

Properties
Form Lyophilized
Storage Buffer lyophilized with PBS, pH 7.4, containing 10 mg/mL BSA and 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL.
Storage Instructions The lyophilized product remains stable up to 2 year at -20°C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freezing and thawing cycles.
Purification Protein A affinity column
Isotype Mouse IgG2b,κ
Clonality Monoclonal
Clone ID 5A8E5

Examples
  • THE™ DYKDDDDK Tag Antibody [iFluor 488], MAb, Mouse
    • THE™ DYKDDDDK Tag Antibody [iFluor 488], MAb, Mouse

    Flow cytometric analysis of non-transfected CHO cells (Black) or Flag-tagged protein transfected CHO cells (Red)
    using THETM DYKDDDDK Tag Antibody [iFluor 488], mAb, Mouse (A01809-100, 4 µg/ml).

  • THE™ DYKDDDDK Tag Antibody [iFluor 488], MAb, Mouse
    • THE™ DYKDDDDK Tag Antibody [iFluor 488], MAb, Mouse

    Immunocytochemistry/Immunofluorescence analysis of non-transfected CHO cells (a) or Flag-tagged protein transfected CHO cells (b)
    using THETM DYKDDDDK Tag Antibody [iFluor 488], mAb, Mouse (A01809-100, 4 µg/ml).


Background
Target Background The Flag tag is a polypeptide of with the amino acid sequence DYKDDDDK amino acid sequence that can be added to a target protein using recombinant DNA technology. It can be fused to the N-terminus or C-terminus of the protein to facilitate detection and purification. An anti-Flag tag antibody is a useful tool for the analysis of Flag-tagged proteins.THE™ DYKDDDDK Tag Antibody [iFluor 488], mAb, Mouse is THE™ DYKDDDDK Tag Antibody (A00187) conjugated with iFluor 488 under optimal conditions with an F/P ratio of 4-8. It is suitable for detecting the expression level of DYKDDDDK-tagged proteins. iFluor 488 is a bright and photostable fluorescent dye. It is an excellent alternative to Alexa Fluor 488.
Synonyms THE™ DYKDDDDK Tag Antibody [iFluor 488], mAb, Mouse; DYKDDDDK Tag Antibody [iFluor 488], mAb, Mouse; 488 conjugated Flag antibody; 488 conjugated Flag monoclonal antibody;

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.