Stable Cell Line Development Services
GenScript's stable cell line service covers all your needs in the aspects of protein/antibody production, and assay development. Not only have we developed cell lines stable at expression level, but also established stable cell lines ready for assay development. Furthermore, GenScript's CellPower™ platform guarantees over-expressing of gene of interest in any cell hosts.
Outline of Stable Cell Line Services
Stable Research Cell Line (SC1260)
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Stable Production Cell Line (SC1503)
| Services | Description | Deliverables | Timeline |
|---|---|---|---|
| 1. Gene synthesis & Subcloning (Optional) |
|
|
4 weeks |
| 2.Transient expression evaluation |
|
|
2–3 weeks |
| 3.Stable cell line development using mammalian cells |
|
|
21–26 weeks |
Stable Industrial Cell Line (SC1557)
| Services | Description | Deliverables | Timeline |
|---|---|---|---|
| 1. Gene synthesis & Subcloning (Optional) |
|
|
4 weeks |
| 2.Transient expression evaluation |
|
|
2–3 weeks |
| 3.Stable cell line development using mammalian cells |
|
|
21–26 weeks |
| 4.Upstream process development |
|
|
8-10 weeks |
Case Study
 |
Fig. MTX amplification for CHO cells expressing a Fc fusion protein. The expression level of the Fc fusion protein increases more than 40-fold after 3 rounds of genomic amplification using MTX. |
Service package(SC1394A & SC1394B)
| Service Steps | Service Features | Additional Validation Services |
|---|---|---|
|
Subcloning of gene of interest |
|
Membrane receptors: FACS, ELISA & Western Blot |
|
Plasmid preparation |
GPCRs: Ca2+, cAMP, IP-1, radiometric binding assays |
|
|
Lentivirus packaging and production |
Ion Channels: electrophysiology, |
|
|
Transduction & selection |
|
Proteases, kinases, and other enzyme: |
|
Q-PCR validated single(poly)-clone |
Nuclear receptors: reporter gene assays |
|
|
Deliverables: 2 vials of cells, |
Cytokines: proliferation or other relevant assays |
GenScript's promises:
- Delivery or no fee
- Mycoplasma-free cells guaranteed
- Any genes and any host cell lines customized with your own project
- 100% success of developing over 200 stable cell lines in the past 4 years
- Guaranteed expression of your genes validated by specific screening methods designated for your proteins
Refer to Guaranteed Stable Cell Line Service for more details
Service package(SC1394A & SC1394B
| Service Steps | Service Features | Additional Validation Services |
|---|---|---|
|
Subcloning of gene of interest |
|
Membrane receptors: FACS, ELISA & Western Blot |
|
Plasmid preparation |
GPCRs: Ca2+, cAMP, IP-1, radiometric binding assays |
|
|
Lentivirus packaging and production |
Ion Channels: electrophysiology, |
|
|
Transduction & selection |
|
Proteases, kinases, and other enzyme: |
|
Q-PCR validated single(poly)-clone |
Nuclear receptors: reporter gene assays |
|
|
Deliverables: 2 vials of cells, |
Cytokines: proliferation or other relevant assays |
GenScript's promises:
- Delivery or no fee
- Mycoplasma-free cells guaranteed
- Any genes and any host cell lines customized with your own project
- 100% success of developing over 200 stable cell lines in the past 4 years
- Guaranteed expression of your genes validated by specific screening methods designated for your proteins
Refer to Guaranteed Stable Cell Line Service for more details
Quotations and Ordering
- To request a quotation, please download and complete the Quotation Form, and send it back by email or fax.
For our protein production mammalian stable cell line development service, please download
.For our guaranteed stable cell line development service, please give us a
Or refer to Guaranteed Stable Cell Line Service - To order, please send the completed Quotation Form to us by email or fax with a formal PO (Purchase Order) or credit card information. You can also submit PO/credit card information by phone or via our secure online messaging system.
- If you are also submitting samples, please mail them together with a hard copy of the completed Quotation Form to the following address: BioProcess Services, GenScript, 860 Centennial Avenue., Piscataway, NJ 08854, US.
