eve cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	eve
Entrez Gene ID	36039
Full Name	even skipped
Synonyms	10.5,10.9,14.10,20.35,CG2328,Dmel\CG2328,E(eve),EVE,Eve,F,V,VI,dm-eve,eve2,even,l(2)46CFg,l(2)46CFh,l(2)46CFj,l(2)46CFp,l(2)46Ce,l(2)46Cg
General protein information	Preferred Names even skipped Names CG2328 gene product from transcript CG2328-RA CG2328-PA complementation group F eve-PA even-skiped even-skipped evenskip evenskipped group V group VI lethal(2)46Ce
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 46C10-46C10

mRNA and Protein(s)

mRNA	Protein	Name
NM_078946.4	NP_523670.2	even skipped

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

natural variation in a conserved trait-even-skipped (eve) expression at the cellular blastoderm stage of embryonic development in Drosophila melanogaster, was investigated.
Title: Natural variation of the expression pattern of the segmentation gene even-skipped in melanogaster.

Deletion of any individual enhancer reduced Yan occupancy at the other elements, impacting eve expression, cell fate specification, and cardiac function
Title: 3D chromatin interactions organize Yan chromatin occupancy and repression at the even-skipped locus.

Ubiquitous TER94 expression is "replaced" by expression in an eve pattern when Homie is deleted, and this effect is reversed when the PRE is also removed
Title: The Drosophila eve insulator Homie promotes eve expression and protects the adjacent gene from repression by polycomb spreading.

Data strongly suggests that the eve-grn transcriptional code controls axon guidance, in part, by regulating the level of unc-5 expression.
Title: A GATA/homeodomain transcriptional code regulates axon guidance through the Unc-5 receptor.

even-skipped genes were misexpressed in pho mutant embryos
Title: The pleiohomeotic functions as a negative regulator of Drosophila even-skipped gene during embryogenesis.

The following eve gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eve cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09433	NM_078946.4 Latest version!	Drosophila melanogaster even skipped (eve), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

eve ( NM_078946.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523670.2 Drosophila melanogaster even skipped (eve), mRNA.

Price & Availability↑

CloneID	OFa09433
Clone ID Related Accession (Same CDS sequence)	NM_078946.4
Accession Version	NM_078946.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	even skipped
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_078946.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCACGGAT ACCGAACCTA CAACATGGAG AGCCACCATG CCCATCACGA CGCCAGTCCC 
GTGGACCAGA AGCCCCTGGT TGTGGACCTC TTGGCCACCC AGTACGGCAA GCCCCAGACA 
CCGCCTCCCT CGCCAAATGA ATGCCTATCC AGTCCGGATA ACTCCTTGAA CGGCAGCCGC 
GGCTCGGAGA TTCCCGCCGA CCCGTCGGTA CGCCGCTATC GCACCGCCTT CACCCGTGAC 
CAGCTGGGTC GCTTGGAGAA GGAGTTCTAC AAGGAGAACT ACGTGTCCCG TCCCCGTCGC 
TGCGAACTGG CCGCCCAGCT GAACCTCCCG GAGAGCACGA TCAAGGTGTG GTTCCAGAAC 
CGCCGCATGA AGGACAAGCG TCAGAGGATC GCCGTCGCCT GGCCCTACGC AGCCGTCTAC 
TCCGATCCCG CCTTCGCCGC CTCCATCCTC CAGGCCGCCG CCAACAGCGT GGGCATGCCC 
TATCCGCCCT ACGCCCCCGC TGCTGCCGCC GCTGCTGCCG CCGCCGCTGC CGTGGCCACC 
AATCCGATGA TGGCCACCGG AATGCCCCCG ATGGGCATGC CCCAGATGCC CACAATGCAG 
ATGCCCGGAC ACTCGGGACA TGCCGGCCAT CCATCGCCCT ACGGACAGTA CCGCTACACG 
CCCTACCACA TCCCCGCCCG CCCGGCGCCG CCACATCCCG CTGGTCCTCA TATGCATCAT 
CCGCACATGA TGGGATCCAG CGCAACGGGA TCGTCGTACT CCGCCGGTGC CGCCGGCCTT 
TTGGGCGCTC TGCCCTCCGC CACCTGCTAT ACCGGACTGG GTGTGGGTGT GCCCAAGACC 
CAGACGCCGC CGCTGGATCT GCAGTCGTCG TCATCGCCGC ACTCCTCCAC GCTGTCGCTC 
TCGCCAGTGG GATCCGATCA CGCCAAGGTG TTCGACCGCA GTCCAGTGGC TCAATCCGCT 
CCATCAGTTC CTGCTCCCGC TCCACTGACC ACCACCAGCC CGCTGCCCGC TCCAGGCCTC 
CTGATGCCCA GTGCCAAGCG GCCTGCCTCC GACATGTCGC CGCCGCCGAC GACAACTGTG 
ATTGCGGAGC CCAAGCCGAA GCTCTTCAAG CCCTACAAGA CTGAGGCGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523670.2
CDS	97..1227
Translation	MHGYRTYNMESHHAHHDASPVDQKPLVVDLLATQYGKPQTPPPSPNECLSSPDNSLNGSRGSEIPADPSVRRYRTAFTRDQLGRLEKEFYKENYVSRPRRCELAAQLNLPESTIKVWFQNRRMKDKRQRIAVAWPYAAVYSDPAFAASILQAAANSVGMPYPPYAPAAAAAAAAAAAVATNPMMATGMPPMGMPQMPTMQMPGHSGHAGHPSPYGQYRYTPYHIPARPAPPHPAGPHMHHPHMMGSSATGSSYSAGAAGLLGALPSATCYTGLGVGVPKTQTPPLDLQSSSSPHSSTLSLSPVGSDHAKVFDRSPVAQSAPSVPAPAPLTTTSPLPAPGLLMPSAKRPASDMSPPPTTTVIAEPKPKLFKPYKTEA

Target ORF information:

RefSeq Version	NM_078946.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster even skipped (eve), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078946.4

ORF Insert Sequence:

ATGCACGGAT ACCGAACCTA CAACATGGAG AGCCACCATG CCCATCACGA CGCCAGTCCC 
GTGGACCAGA AGCCCCTGGT TGTGGACCTC TTGGCCACCC AGTACGGCAA GCCCCAGACA 
CCGCCTCCCT CGCCAAATGA ATGCCTATCC AGTCCGGATA ACTCCTTGAA CGGCAGCCGC 
GGCTCGGAGA TTCCCGCCGA CCCGTCGGTA CGCCGCTATC GCACCGCCTT CACCCGTGAC 
CAGCTGGGTC GCTTGGAGAA GGAGTTCTAC AAGGAGAACT ACGTGTCCCG TCCCCGTCGC 
TGCGAACTGG CCGCCCAGCT GAACCTCCCG GAGAGCACGA TCAAGGTGTG GTTCCAGAAC 
CGCCGCATGA AGGACAAGCG TCAGAGGATC GCCGTCGCCT GGCCCTACGC AGCCGTCTAC 
TCCGATCCCG CCTTCGCCGC CTCCATCCTC CAGGCCGCCG CCAACAGCGT GGGCATGCCC 
TATCCGCCCT ACGCCCCCGC TGCTGCCGCC GCTGCTGCCG CCGCCGCTGC CGTGGCCACC 
AATCCGATGA TGGCCACCGG AATGCCCCCG ATGGGCATGC CCCAGATGCC CACAATGCAG 
ATGCCCGGAC ACTCGGGACA TGCCGGCCAT CCATCGCCCT ACGGACAGTA CCGCTACACG 
CCCTACCACA TCCCCGCCCG CCCGGCGCCG CCACATCCCG CTGGTCCTCA TATGCATCAT 
CCGCACATGA TGGGATCCAG CGCAACGGGA TCGTCGTACT CCGCCGGTGC CGCCGGCCTT 
TTGGGCGCTC TGCCCTCCGC CACCTGCTAT ACCGGACTGG GTGTGGGTGT GCCCAAGACC 
CAGACGCCGC CGCTGGATCT GCAGTCGTCG TCATCGCCGC ACTCCTCCAC GCTGTCGCTC 
TCGCCAGTGG GATCCGATCA CGCCAAGGTG TTCGACCGCA GTCCAGTGGC TCAATCCGCT 
CCATCAGTTC CTGCTCCCGC TCCACTGACC ACCACCAGCC CGCTGCCCGC TCCAGGCCTC 
CTGATGCCCA GTGCCAAGCG GCCTGCCTCC GACATGTCGC CGCCGCCGAC GACAACTGTG 
ATTGCGGAGC CCAAGCCGAA GCTCTTCAAG CCCTACAAGA CTGAGGCGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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eve cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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