blow cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	blow
Entrez Gene ID	35694
Full Name	blown fuse
Synonyms	Blow,CG1363,Dmel\CG1363,l(2)43Eb
General protein information	Preferred Names blown fuse Names CG1363 gene product from transcript CG1363-RA CG1363-PA CG1363-PB CG1363-PD blow-PA blow-PB blow-PD blown-fuse blownfuse
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 43E1-43E3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001169603.1	NP_001163074.1	blown fuse, isoform B
NM_001169602.2	NP_001163073.2	blown fuse, isoform D
NM_165542.3	NP_724580.1	blown fuse, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The dynamics of the PLS is controlled by Blown fuse (Blow), a cytoplasmic protein required for myoblast fusion but whose molecular function has been elusive.
Title: Competition between Blown fuse and WASP for WIP binding regulates the dynamics of WASP-dependent actin polymerization in vivo.

a model for Blow in which this protein is involved in at least two clearly differing processes during Drosophila muscle formation, namely somatic myoblast fusion on the one hand and stretching and outgrowth of circular visceral muscles on the other.
Title: Blown fuse regulates stretching and outgrowth but not myoblast fusion of the circular visceral muscles in Drosophila.

kette interacts genetically with blown fuse (blow) which is known to be required to proceed from prefusion complexes to the formation of the electron dense plaques
Title: kette and blown fuse interact genetically during the second fusion step of myogenesis in Drosophila.

The following blow gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the blow cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa09161	NM_001169602.2 Latest version!	Drosophila melanogaster blown fuse (blow), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa09161	NM_165542.3 Latest version!	Drosophila melanogaster blown fuse (blow), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa09162	NM_001169603.1 Latest version!	Drosophila melanogaster blown fuse (blow), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

blow ( NM_001169602.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163073.2 Drosophila melanogaster blown fuse (blow), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa09161
Clone ID Related Accession (Same CDS sequence)	NM_001169602.2 , NM_165542.3
Accession Version	NM_001169602.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	blown fuse, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001169602.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATCCGA AACCAACAAC TACCAAGCTG GCCGAAATTG AGCAGTTTTT GTTGGATGTC 
CGCGCCCACT TCCGAGCCTC GCTGGAGACC TCGGAGTACG AGAACACCTC GAACCTATTC 
CATGTGACGG CCGCCGAGCA GACCGCCGCT CTTCTGGCCC GCTGCGAAAT CCTGCTGGCT 
GCAGCCAGTT CTGCATCGGG ATCGGGATCG GGACTGGAAC TGGGATCAGG GCTGGGATGC 
GCAGACGAGC AGGCGGCAGC TAACCATCGT TCCAGCCTGG TCGACTGTGT CGATAGCAAG 
GTGGGCGCGG AGACCGGTCC CAGCGGTTAT CTGGAGATGC TTGCCTCCCC GTCACCCTCG 
AAAAGCTCAC TGTCCGCCTC GCGGGAGTAC ATTGATTTGG GCAGCAGCGG ATCCCTGGAT 
CGTTGCTCCG TCAATCAACC GGTTCCGGCA GCGAAGGACA AGGAGCCACA TCAGCAGCTC 
TACGAGATTT GCCAGGGCCA GGGAGCGGGC CAGGACGAGG ATGAGGCGCA ACAGGCGATC 
ACCTTTACCC ACGAGGTGTT CATTGACTGC CCGTATGCAG ATCTGCCCGC TGGCCACTTG 
TCCCTGCAGA GGTCAACAAA GTACGGACAG CTGCAGAGGA TTGTGCCCAA GCGGCTGTTC 
TTCGACCAGA CCCGCAAGTG CTACTGTGGC ATCCTCAACG ATTGGATGCT GTGCTATGCC 
GACGGCCCGA CCGCCTGTCG ACCCAGCAGC ACGCTCTACT TGAAGAGCTC CTCCATCGAG 
ATTGAACACT TCGGAGAGGG GAAGCGGCGC GACATCTGCT TCCAGATCAC AACAGACGAC 
CCCAACAAGA AGTTCGTGTA CCAGGCCAAC AACGAAGTCG ATGCCAAAGA GTGGATACAC 
GCCATTGAGG CGGCCATCCG CGGAGATGCT GGTGCTCATT CAGCGTTAAA GACTCCACCG 
CCCCGGAAAC TGCCCACGCC GCCGGTCCAA AAGAAATCCA CCTACACAGC AGCGGATATC 
ATCTACGAGG AGCCCTCGCC CGTGTATGGT CTCATAGACT CCTCCTCCAT GGTGCTCCCT 
AAGCTGCCGG AGAAAAGCAA CAGCCCAAGC GCCCTCGCCC GACGTTTCGA GTACGATGTG 
CCCAAATGCC CGGCACAGCC GCTGCAGGAC ACCTCCTCCA GGGTGGCATC TGTAAGTGGG 
GAGCTGGAAT CCCTGGGACT ACTCAACGAG GGAGATATTA AGGTGCAACC GCAGCCTTCC 
AGTTTGCACG GAAGCATATC GCTGGACTTC CAGGCCAGGG TGAAAACAGT GCACAGTGAG 
CTCTCCACCC AGTTATCAGC GGCCGGTCCC AGTCCGGATC GTCTGAAAAA GGCAGCGAAA 
AAGAGCTATT CGAATGGTAG TGAACCGGAG ATGCTCTCTC CCCTGACCAG CCCCGTCCAA 
ACGCCCACCA AGGATCAGAA GAAGCAGCGC AAGTCAACCA CCTCACCGCA ATCCAGTCCG 
GTCAGCAAGG ACTGCGACAA GCTGGCACGA AACTGGTTCC TCAGTCGCCT TAACAAGAGC 
ACCGGATTGA AGGCGACCAA CACGGGCAGT GCATCACCAA CCAACGCCAA GTGCAAGCAG 
AGCGAGAAGG AGAACCTGCT GACGGAAGCG GAGCTGGGCG AGGGCTATGA TGCAAGTCCT 
GGTGATCGGT ATCACGGCCT GCCGGGCAGC CCGCCCACGT CGCCGTGTCT GAAGGCGGAG 
GCCAAGAGCA AGGTGAACAT GATTATCAAC CAGTTTGAGA GTAGCGGTCA CCTGTCCACC 
ATGTTCAGCG TGGAAGCTTT GGCCGCCAAT GCACTCGCCT CGCTGACATC CTTCTGCGAA 
AGTGATGGTT GCAACAACTA CGAGCCAATC ATGCCCCTGG CCACGACTCC CAGCAGCTTT 
CTCAAGAAGG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163073.2
CDS	376..2310
Translation	MNPKPTTTKLAEIEQFLLDVRAHFRASLETSEYENTSNLFHVTAAEQTAALLARCEILLAAASSASGSGSGLELGSGLGCADEQAAANHRSSLVDCVDSKVGAETGPSGYLEMLASPSPSKSSLSASREYIDLGSSGSLDRCSVNQPVPAAKDKEPHQQLYEICQGQGAGQDEDEAQQAITFTHEVFIDCPYADLPAGHLSLQRSTKYGQLQRIVPKRLFFDQTRKCYCGILNDWMLCYADGPTACRPSSTLYLKSSSIEIEHFGEGKRRDICFQITTDDPNKKFVYQANNEVDAKEWIHAIEAAIRGDAGAHSALKTPPPRKLPTPPVQKKSTYTAADIIYEEPSPVYGLIDSSSMVLPKLPEKSNSPSALARRFEYDVPKCPAQPLQDTSSRVASVSGELESLGLLNEGDIKVQPQPSSLHGSISLDFQARVKTVHSELSTQLSAAGPSPDRLKKAAKKSYSNGSEPEMLSPLTSPVQTPTKDQKKQRKSTTSPQSSPVSKDCDKLARNWFLSRLNKSTGLKATNTGSASPTNAKCKQSEKENLLTEAELGEGYDASPGDRYHGLPGSPPTSPCLKAEAKSKVNMIINQFESSGHLSTMFSVEALAANALASLTSFCESDGCNNYEPIMPLATTPSSFLKKV

Target ORF information:

RefSeq Version	NM_001169602.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster blown fuse (blow), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169602.2

ORF Insert Sequence:

ATGAATCCGA AACCAACAAC TACCAAGCTG GCCGAAATTG AGCAGTTTTT GTTGGATGTC 
CGCGCCCACT TCCGAGCCTC GCTGGAGACC TCGGAGTACG AGAACACCTC GAACCTATTC 
CATGTGACGG CCGCCGAGCA GACCGCCGCT CTTCTGGCCC GCTGCGAAAT CCTGCTGGCT 
GCAGCCAGTT CTGCATCGGG ATCGGGATCG GGACTGGAAC TGGGATCAGG GCTGGGATGC 
GCAGACGAGC AGGCGGCAGC TAACCATCGT TCCAGCCTGG TCGACTGTGT CGATAGCAAG 
GTGGGCGCGG AGACCGGTCC CAGCGGTTAT CTGGAGATGC TTGCCTCCCC GTCACCCTCG 
AAAAGCTCAC TGTCCGCCTC GCGGGAGTAC ATTGATTTGG GCAGCAGCGG ATCCCTGGAT 
CGTTGCTCCG TCAATCAACC GGTTCCGGCA GCGAAGGACA AGGAGCCACA TCAGCAGCTC 
TACGAGATTT GCCAGGGCCA GGGAGCGGGC CAGGACGAGG ATGAGGCGCA ACAGGCGATC 
ACCTTTACCC ACGAGGTGTT CATTGACTGC CCGTATGCAG ATCTGCCCGC TGGCCACTTG 
TCCCTGCAGA GGTCAACAAA GTACGGACAG CTGCAGAGGA TTGTGCCCAA GCGGCTGTTC 
TTCGACCAGA CCCGCAAGTG CTACTGTGGC ATCCTCAACG ATTGGATGCT GTGCTATGCC 
GACGGCCCGA CCGCCTGTCG ACCCAGCAGC ACGCTCTACT TGAAGAGCTC CTCCATCGAG 
ATTGAACACT TCGGAGAGGG GAAGCGGCGC GACATCTGCT TCCAGATCAC AACAGACGAC 
CCCAACAAGA AGTTCGTGTA CCAGGCCAAC AACGAAGTCG ATGCCAAAGA GTGGATACAC 
GCCATTGAGG CGGCCATCCG CGGAGATGCT GGTGCTCATT CAGCGTTAAA GACTCCACCG 
CCCCGGAAAC TGCCCACGCC GCCGGTCCAA AAGAAATCCA CCTACACAGC AGCGGATATC 
ATCTACGAGG AGCCCTCGCC CGTGTATGGT CTCATAGACT CCTCCTCCAT GGTGCTCCCT 
AAGCTGCCGG AGAAAAGCAA CAGCCCAAGC GCCCTCGCCC GACGTTTCGA GTACGATGTG 
CCCAAATGCC CGGCACAGCC GCTGCAGGAC ACCTCCTCCA GGGTGGCATC TGTAAGTGGG 
GAGCTGGAAT CCCTGGGACT ACTCAACGAG GGAGATATTA AGGTGCAACC GCAGCCTTCC 
AGTTTGCACG GAAGCATATC GCTGGACTTC CAGGCCAGGG TGAAAACAGT GCACAGTGAG 
CTCTCCACCC AGTTATCAGC GGCCGGTCCC AGTCCGGATC GTCTGAAAAA GGCAGCGAAA 
AAGAGCTATT CGAATGGTAG TGAACCGGAG ATGCTCTCTC CCCTGACCAG CCCCGTCCAA 
ACGCCCACCA AGGATCAGAA GAAGCAGCGC AAGTCAACCA CCTCACCGCA ATCCAGTCCG 
GTCAGCAAGG ACTGCGACAA GCTGGCACGA AACTGGTTCC TCAGTCGCCT TAACAAGAGC 
ACCGGATTGA AGGCGACCAA CACGGGCAGT GCATCACCAA CCAACGCCAA GTGCAAGCAG 
AGCGAGAAGG AGAACCTGCT GACGGAAGCG GAGCTGGGCG AGGGCTATGA TGCAAGTCCT 
GGTGATCGGT ATCACGGCCT GCCGGGCAGC CCGCCCACGT CGCCGTGTCT GAAGGCGGAG 
GCCAAGAGCA AGGTGAACAT GATTATCAAC CAGTTTGAGA GTAGCGGTCA CCTGTCCACC 
ATGTTCAGCG TGGAAGCTTT GGCCGCCAAT GCACTCGCCT CGCTGACATC CTTCTGCGAA 
AGTGATGGTT GCAACAACTA CGAGCCAATC ATGCCCCTGG CCACGACTCC CAGCAGCTTT 
CTCAAGAAGG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

blow ( NM_165542.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724580.1 Drosophila melanogaster blown fuse (blow), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa09161
Clone ID Related Accession (Same CDS sequence)	NM_001169602.2 , NM_165542.3
Accession Version	NM_165542.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	blown fuse, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165542.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATCCGA AACCAACAAC TACCAAGCTG GCCGAAATTG AGCAGTTTTT GTTGGATGTC 
CGCGCCCACT TCCGAGCCTC GCTGGAGACC TCGGAGTACG AGAACACCTC GAACCTATTC 
CATGTGACGG CCGCCGAGCA GACCGCCGCT CTTCTGGCCC GCTGCGAAAT CCTGCTGGCT 
GCAGCCAGTT CTGCATCGGG ATCGGGATCG GGACTGGAAC TGGGATCAGG GCTGGGATGC 
GCAGACGAGC AGGCGGCAGC TAACCATCGT TCCAGCCTGG TCGACTGTGT CGATAGCAAG 
GTGGGCGCGG AGACCGGTCC CAGCGGTTAT CTGGAGATGC TTGCCTCCCC GTCACCCTCG 
AAAAGCTCAC TGTCCGCCTC GCGGGAGTAC ATTGATTTGG GCAGCAGCGG ATCCCTGGAT 
CGTTGCTCCG TCAATCAACC GGTTCCGGCA GCGAAGGACA AGGAGCCACA TCAGCAGCTC 
TACGAGATTT GCCAGGGCCA GGGAGCGGGC CAGGACGAGG ATGAGGCGCA ACAGGCGATC 
ACCTTTACCC ACGAGGTGTT CATTGACTGC CCGTATGCAG ATCTGCCCGC TGGCCACTTG 
TCCCTGCAGA GGTCAACAAA GTACGGACAG CTGCAGAGGA TTGTGCCCAA GCGGCTGTTC 
TTCGACCAGA CCCGCAAGTG CTACTGTGGC ATCCTCAACG ATTGGATGCT GTGCTATGCC 
GACGGCCCGA CCGCCTGTCG ACCCAGCAGC ACGCTCTACT TGAAGAGCTC CTCCATCGAG 
ATTGAACACT TCGGAGAGGG GAAGCGGCGC GACATCTGCT TCCAGATCAC AACAGACGAC 
CCCAACAAGA AGTTCGTGTA CCAGGCCAAC AACGAAGTCG ATGCCAAAGA GTGGATACAC 
GCCATTGAGG CGGCCATCCG CGGAGATGCT GGTGCTCATT CAGCGTTAAA GACTCCACCG 
CCCCGGAAAC TGCCCACGCC GCCGGTCCAA AAGAAATCCA CCTACACAGC AGCGGATATC 
ATCTACGAGG AGCCCTCGCC CGTGTATGGT CTCATAGACT CCTCCTCCAT GGTGCTCCCT 
AAGCTGCCGG AGAAAAGCAA CAGCCCAAGC GCCCTCGCCC GACGTTTCGA GTACGATGTG 
CCCAAATGCC CGGCACAGCC GCTGCAGGAC ACCTCCTCCA GGGTGGCATC TGTAAGTGGG 
GAGCTGGAAT CCCTGGGACT ACTCAACGAG GGAGATATTA AGGTGCAACC GCAGCCTTCC 
AGTTTGCACG GAAGCATATC GCTGGACTTC CAGGCCAGGG TGAAAACAGT GCACAGTGAG 
CTCTCCACCC AGTTATCAGC GGCCGGTCCC AGTCCGGATC GTCTGAAAAA GGCAGCGAAA 
AAGAGCTATT CGAATGGTAG TGAACCGGAG ATGCTCTCTC CCCTGACCAG CCCCGTCCAA 
ACGCCCACCA AGGATCAGAA GAAGCAGCGC AAGTCAACCA CCTCACCGCA ATCCAGTCCG 
GTCAGCAAGG ACTGCGACAA GCTGGCACGA AACTGGTTCC TCAGTCGCCT TAACAAGAGC 
ACCGGATTGA AGGCGACCAA CACGGGCAGT GCATCACCAA CCAACGCCAA GTGCAAGCAG 
AGCGAGAAGG AGAACCTGCT GACGGAAGCG GAGCTGGGCG AGGGCTATGA TGCAAGTCCT 
GGTGATCGGT ATCACGGCCT GCCGGGCAGC CCGCCCACGT CGCCGTGTCT GAAGGCGGAG 
GCCAAGAGCA AGGTGAACAT GATTATCAAC CAGTTTGAGA GTAGCGGTCA CCTGTCCACC 
ATGTTCAGCG TGGAAGCTTT GGCCGCCAAT GCACTCGCCT CGCTGACATC CTTCTGCGAA 
AGTGATGGTT GCAACAACTA CGAGCCAATC ATGCCCCTGG CCACGACTCC CAGCAGCTTT 
CTCAAGAAGG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724580.1
CDS	396..2330
Translation	MNPKPTTTKLAEIEQFLLDVRAHFRASLETSEYENTSNLFHVTAAEQTAALLARCEILLAAASSASGSGSGLELGSGLGCADEQAAANHRSSLVDCVDSKVGAETGPSGYLEMLASPSPSKSSLSASREYIDLGSSGSLDRCSVNQPVPAAKDKEPHQQLYEICQGQGAGQDEDEAQQAITFTHEVFIDCPYADLPAGHLSLQRSTKYGQLQRIVPKRLFFDQTRKCYCGILNDWMLCYADGPTACRPSSTLYLKSSSIEIEHFGEGKRRDICFQITTDDPNKKFVYQANNEVDAKEWIHAIEAAIRGDAGAHSALKTPPPRKLPTPPVQKKSTYTAADIIYEEPSPVYGLIDSSSMVLPKLPEKSNSPSALARRFEYDVPKCPAQPLQDTSSRVASVSGELESLGLLNEGDIKVQPQPSSLHGSISLDFQARVKTVHSELSTQLSAAGPSPDRLKKAAKKSYSNGSEPEMLSPLTSPVQTPTKDQKKQRKSTTSPQSSPVSKDCDKLARNWFLSRLNKSTGLKATNTGSASPTNAKCKQSEKENLLTEAELGEGYDASPGDRYHGLPGSPPTSPCLKAEAKSKVNMIINQFESSGHLSTMFSVEALAANALASLTSFCESDGCNNYEPIMPLATTPSSFLKKV

Target ORF information:

RefSeq Version	NM_165542.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster blown fuse (blow), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165542.3

ORF Insert Sequence:

ATGAATCCGA AACCAACAAC TACCAAGCTG GCCGAAATTG AGCAGTTTTT GTTGGATGTC 
CGCGCCCACT TCCGAGCCTC GCTGGAGACC TCGGAGTACG AGAACACCTC GAACCTATTC 
CATGTGACGG CCGCCGAGCA GACCGCCGCT CTTCTGGCCC GCTGCGAAAT CCTGCTGGCT 
GCAGCCAGTT CTGCATCGGG ATCGGGATCG GGACTGGAAC TGGGATCAGG GCTGGGATGC 
GCAGACGAGC AGGCGGCAGC TAACCATCGT TCCAGCCTGG TCGACTGTGT CGATAGCAAG 
GTGGGCGCGG AGACCGGTCC CAGCGGTTAT CTGGAGATGC TTGCCTCCCC GTCACCCTCG 
AAAAGCTCAC TGTCCGCCTC GCGGGAGTAC ATTGATTTGG GCAGCAGCGG ATCCCTGGAT 
CGTTGCTCCG TCAATCAACC GGTTCCGGCA GCGAAGGACA AGGAGCCACA TCAGCAGCTC 
TACGAGATTT GCCAGGGCCA GGGAGCGGGC CAGGACGAGG ATGAGGCGCA ACAGGCGATC 
ACCTTTACCC ACGAGGTGTT CATTGACTGC CCGTATGCAG ATCTGCCCGC TGGCCACTTG 
TCCCTGCAGA GGTCAACAAA GTACGGACAG CTGCAGAGGA TTGTGCCCAA GCGGCTGTTC 
TTCGACCAGA CCCGCAAGTG CTACTGTGGC ATCCTCAACG ATTGGATGCT GTGCTATGCC 
GACGGCCCGA CCGCCTGTCG ACCCAGCAGC ACGCTCTACT TGAAGAGCTC CTCCATCGAG 
ATTGAACACT TCGGAGAGGG GAAGCGGCGC GACATCTGCT TCCAGATCAC AACAGACGAC 
CCCAACAAGA AGTTCGTGTA CCAGGCCAAC AACGAAGTCG ATGCCAAAGA GTGGATACAC 
GCCATTGAGG CGGCCATCCG CGGAGATGCT GGTGCTCATT CAGCGTTAAA GACTCCACCG 
CCCCGGAAAC TGCCCACGCC GCCGGTCCAA AAGAAATCCA CCTACACAGC AGCGGATATC 
ATCTACGAGG AGCCCTCGCC CGTGTATGGT CTCATAGACT CCTCCTCCAT GGTGCTCCCT 
AAGCTGCCGG AGAAAAGCAA CAGCCCAAGC GCCCTCGCCC GACGTTTCGA GTACGATGTG 
CCCAAATGCC CGGCACAGCC GCTGCAGGAC ACCTCCTCCA GGGTGGCATC TGTAAGTGGG 
GAGCTGGAAT CCCTGGGACT ACTCAACGAG GGAGATATTA AGGTGCAACC GCAGCCTTCC 
AGTTTGCACG GAAGCATATC GCTGGACTTC CAGGCCAGGG TGAAAACAGT GCACAGTGAG 
CTCTCCACCC AGTTATCAGC GGCCGGTCCC AGTCCGGATC GTCTGAAAAA GGCAGCGAAA 
AAGAGCTATT CGAATGGTAG TGAACCGGAG ATGCTCTCTC CCCTGACCAG CCCCGTCCAA 
ACGCCCACCA AGGATCAGAA GAAGCAGCGC AAGTCAACCA CCTCACCGCA ATCCAGTCCG 
GTCAGCAAGG ACTGCGACAA GCTGGCACGA AACTGGTTCC TCAGTCGCCT TAACAAGAGC 
ACCGGATTGA AGGCGACCAA CACGGGCAGT GCATCACCAA CCAACGCCAA GTGCAAGCAG 
AGCGAGAAGG AGAACCTGCT GACGGAAGCG GAGCTGGGCG AGGGCTATGA TGCAAGTCCT 
GGTGATCGGT ATCACGGCCT GCCGGGCAGC CCGCCCACGT CGCCGTGTCT GAAGGCGGAG 
GCCAAGAGCA AGGTGAACAT GATTATCAAC CAGTTTGAGA GTAGCGGTCA CCTGTCCACC 
ATGTTCAGCG TGGAAGCTTT GGCCGCCAAT GCACTCGCCT CGCTGACATC CTTCTGCGAA 
AGTGATGGTT GCAACAACTA CGAGCCAATC ATGCCCCTGG CCACGACTCC CAGCAGCTTT 
CTCAAGAAGG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

blow ( NM_001169603.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163074.1 Drosophila melanogaster blown fuse (blow), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa09162
Clone ID Related Accession (Same CDS sequence)	NM_001169603.1
Accession Version	NM_001169603.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	blown fuse, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATCAGGG GAATAGAGGA GCACGTACAT ATATATGTCC GCGCCCACTT CCGAGCCTCG 
CTGGAGACCT CGGAGTACGA GAACACCTCG AACCTATTCC ATGTGACGGC CGCCGAGCAG 
ACCGCCGCTC TTCTGGCCCG CTGCGAAATC CTGCTGGCTG CAGCCAGTTC TGCATCGGGA 
TCGGGATCGG GACTGGAACT GGGATCAGGG CTGGGATGCG CAGACGAGCA GGCGGCAGCT 
AACCATCGTT CCAGCCTGGT CGACTGTGTC GATAGCAAGG TGGGCGCGGA GACCGGTCCC 
AGCGGTTATC TGGAGATGCT TGCCTCCCCG TCACCCTCGA AAAGCTCACT GTCCGCCTCG 
CGGGAGTACA TTGATTTGGG CAGCAGCGGA TCCCTGGATC GTTGCTCCGT CAATCAACCG 
GTTCCGGCAG CGAAGGACAA GGAGCCACAT CAGCAGCTCT ACGAGATTTG CCAGGGCCAG 
GGAGCGGGCC AGGACGAGGA TGAGGCGCAA CAGGCGATCA CCTTTACCCA CGAGGTGTTC 
ATTGACTGCC CGTATGCAGA TCTGCCCGCT GGCCACTTGT CCCTGCAGAG GTCAACAAAG 
TACGGACAGC TGCAGAGGAT TGTGCCCAAG CGGCTGTTCT TCGACCAGAC CCGCAAGTGC 
TACTGTGGCA TCCTCAACGA TTGGATGCTG TGCTATGCCG ACGGCCCGAC CGCCTGTCGA 
CCCAGCAGCA CGCTCTACTT GAAGAGCTCC TCCATCGAGA TTGAACACTT CGGAGAGGGG 
AAGCGGCGCG ACATCTGCTT CCAGATCACA ACAGACGACC CCAACAAGAA GTTCGTGTAC 
CAGGCCAACA ACGAAGTCGA TGCCAAAGAG TGGATACACG CCATTGAGGC GGCCATCCGC 
GGAGATGCTG GTGCTCATTC AGCGTTAAAG ACTCCACCGC CCCGGAAACT GCCCACGCCG 
CCGGTCCAAA AGAAATCCAC CTACACAGCA GCGGATATCA TCTACGAGGA GCCCTCGCCC 
GTGTATGGTC TCATAGACTC CTCCTCCATG GTGCTCCCTA AGCTGCCGGA GAAAAGCAAC 
AGCCCAAGCG CCCTCGCCCG ACGTTTCGAG TACGATGTGC CCAAATGCCC GGCACAGCCG 
CTGCAGGACA CCTCCTCCAG GGTGGCATCT GTAAGTGGGG AGCTGGAATC CCTGGGACTA 
CTCAACGAGG GAGATATTAA GGTGCAACCG CAGCCTTCCA GTTTGCACGG AAGCATATCG 
CTGGACTTCC AGGCCAGGGT GAAAACAGTG CACAGTGAGC TCTCCACCCA GTTATCAGCG 
GCCGGTCCCA GTCCGGATCG TCTGAAAAAG GCAGCGAAAA AGAGCTATTC GAATGGTAGT 
GAACCGGAGA TGCTCTCTCC CCTGACCAGC CCCGTCCAAA CGCCCACCAA GGATCAGAAG 
AAGCAGCGCA AGTCAACCAC CTCACCGCAA TCCAGTCCGG TCAGCAAGGA CTGCGACAAG 
CTGGCACGAA ACTGGTTCCT CAGTCGCCTT AACAAGAGCA CCGGATTGAA GGCGACCAAC 
ACGGGCAGTG CATCACCAAC CAACGCCAAG TGCAAGCAGA GCGAGAAGGA GAACCTGCTG 
ACGGAAGCGG AGCTGGGCGA GGGCTATGAT GCAAGTCCTG GTGATCGGTA TCACGGCCTG 
CCGGGCAGCC CGCCCACGTC GCCGTGTCTG AAGGCGGAGG CCAAGAGCAA GGTGAACATG 
ATTATCAACC AGTTTGAGAG TAGCGGTCAC CTGTCCACCA TGTTCAGCGT GGAAGCTTTG 
GCCGCCAATG CACTCGCCTC GCTGACATCC TTCTGCGAAA GTGATGGTTG CAACAACTAC 
GAGCCAATCA TGCCCCTGGC CACGACTCCC AGCAGCTTTC TCAAGAAGGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163074.1
CDS	55..1968
Translation	MIRGIEEHVHIYVRAHFRASLETSEYENTSNLFHVTAAEQTAALLARCEILLAAASSASGSGSGLELGSGLGCADEQAAANHRSSLVDCVDSKVGAETGPSGYLEMLASPSPSKSSLSASREYIDLGSSGSLDRCSVNQPVPAAKDKEPHQQLYEICQGQGAGQDEDEAQQAITFTHEVFIDCPYADLPAGHLSLQRSTKYGQLQRIVPKRLFFDQTRKCYCGILNDWMLCYADGPTACRPSSTLYLKSSSIEIEHFGEGKRRDICFQITTDDPNKKFVYQANNEVDAKEWIHAIEAAIRGDAGAHSALKTPPPRKLPTPPVQKKSTYTAADIIYEEPSPVYGLIDSSSMVLPKLPEKSNSPSALARRFEYDVPKCPAQPLQDTSSRVASVSGELESLGLLNEGDIKVQPQPSSLHGSISLDFQARVKTVHSELSTQLSAAGPSPDRLKKAAKKSYSNGSEPEMLSPLTSPVQTPTKDQKKQRKSTTSPQSSPVSKDCDKLARNWFLSRLNKSTGLKATNTGSASPTNAKCKQSEKENLLTEAELGEGYDASPGDRYHGLPGSPPTSPCLKAEAKSKVNMIINQFESSGHLSTMFSVEALAANALASLTSFCESDGCNNYEPIMPLATTPSSFLKKV

Target ORF information:

RefSeq Version	NM_001169603.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster blown fuse (blow), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169603.1

ORF Insert Sequence:

ATGATCAGGG GAATAGAGGA GCACGTACAT ATATATGTCC GCGCCCACTT CCGAGCCTCG 
CTGGAGACCT CGGAGTACGA GAACACCTCG AACCTATTCC ATGTGACGGC CGCCGAGCAG 
ACCGCCGCTC TTCTGGCCCG CTGCGAAATC CTGCTGGCTG CAGCCAGTTC TGCATCGGGA 
TCGGGATCGG GACTGGAACT GGGATCAGGG CTGGGATGCG CAGACGAGCA GGCGGCAGCT 
AACCATCGTT CCAGCCTGGT CGACTGTGTC GATAGCAAGG TGGGCGCGGA GACCGGTCCC 
AGCGGTTATC TGGAGATGCT TGCCTCCCCG TCACCCTCGA AAAGCTCACT GTCCGCCTCG 
CGGGAGTACA TTGATTTGGG CAGCAGCGGA TCCCTGGATC GTTGCTCCGT CAATCAACCG 
GTTCCGGCAG CGAAGGACAA GGAGCCACAT CAGCAGCTCT ACGAGATTTG CCAGGGCCAG 
GGAGCGGGCC AGGACGAGGA TGAGGCGCAA CAGGCGATCA CCTTTACCCA CGAGGTGTTC 
ATTGACTGCC CGTATGCAGA TCTGCCCGCT GGCCACTTGT CCCTGCAGAG GTCAACAAAG 
TACGGACAGC TGCAGAGGAT TGTGCCCAAG CGGCTGTTCT TCGACCAGAC CCGCAAGTGC 
TACTGTGGCA TCCTCAACGA TTGGATGCTG TGCTATGCCG ACGGCCCGAC CGCCTGTCGA 
CCCAGCAGCA CGCTCTACTT GAAGAGCTCC TCCATCGAGA TTGAACACTT CGGAGAGGGG 
AAGCGGCGCG ACATCTGCTT CCAGATCACA ACAGACGACC CCAACAAGAA GTTCGTGTAC 
CAGGCCAACA ACGAAGTCGA TGCCAAAGAG TGGATACACG CCATTGAGGC GGCCATCCGC 
GGAGATGCTG GTGCTCATTC AGCGTTAAAG ACTCCACCGC CCCGGAAACT GCCCACGCCG 
CCGGTCCAAA AGAAATCCAC CTACACAGCA GCGGATATCA TCTACGAGGA GCCCTCGCCC 
GTGTATGGTC TCATAGACTC CTCCTCCATG GTGCTCCCTA AGCTGCCGGA GAAAAGCAAC 
AGCCCAAGCG CCCTCGCCCG ACGTTTCGAG TACGATGTGC CCAAATGCCC GGCACAGCCG 
CTGCAGGACA CCTCCTCCAG GGTGGCATCT GTAAGTGGGG AGCTGGAATC CCTGGGACTA 
CTCAACGAGG GAGATATTAA GGTGCAACCG CAGCCTTCCA GTTTGCACGG AAGCATATCG 
CTGGACTTCC AGGCCAGGGT GAAAACAGTG CACAGTGAGC TCTCCACCCA GTTATCAGCG 
GCCGGTCCCA GTCCGGATCG TCTGAAAAAG GCAGCGAAAA AGAGCTATTC GAATGGTAGT 
GAACCGGAGA TGCTCTCTCC CCTGACCAGC CCCGTCCAAA CGCCCACCAA GGATCAGAAG 
AAGCAGCGCA AGTCAACCAC CTCACCGCAA TCCAGTCCGG TCAGCAAGGA CTGCGACAAG 
CTGGCACGAA ACTGGTTCCT CAGTCGCCTT AACAAGAGCA CCGGATTGAA GGCGACCAAC 
ACGGGCAGTG CATCACCAAC CAACGCCAAG TGCAAGCAGA GCGAGAAGGA GAACCTGCTG 
ACGGAAGCGG AGCTGGGCGA GGGCTATGAT GCAAGTCCTG GTGATCGGTA TCACGGCCTG 
CCGGGCAGCC CGCCCACGTC GCCGTGTCTG AAGGCGGAGG CCAAGAGCAA GGTGAACATG 
ATTATCAACC AGTTTGAGAG TAGCGGTCAC CTGTCCACCA TGTTCAGCGT GGAAGCTTTG 
GCCGCCAATG CACTCGCCTC GCTGACATCC TTCTGCGAAA GTGATGGTTG CAACAACTAC 
GAGCCAATCA TGCCCCTGGC CACGACTCCC AGCAGCTTTC TCAAGAAGGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

blow cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報