chif cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	chif
Entrez Gene ID	34974
Full Name	chiffon
Synonyms	BG:DS09218.1,BG:DS09218.2,CG5813,CG5817,Chif,DS09218.1,Dbf4,Dmel\CG5813,chf,fs(2)chiffon
General protein information	Preferred Names chiffon Names CG5813 gene product from transcript CG5813-RA CG5813-PA CG5813-PB CG5813-PD chif-PA chif-PB chif-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 35F1-35F12\|2-53 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_078859.4	NP_523583.2	chiffon, isoform A
NM_001273570.2	NP_001260499.1	chiffon, isoform D
NM_165156.3	NP_723965.1	chiffon, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Cdc7 has a role in endoreplication independent of Chiffon in Drosophila
Title: Characterization of a Drosophila ortholog of the Cdc7 kinase: a role for Cdc7 in endoreplication independent of Chiffon.

Data suggest a model in which ACE3 and ori-beta nucleate the formation of a ORC2-containing chromatin structure that spreads along the chromosome in a mechanism dependent upon chiffon.
Title: Sequence requirements for function of the Drosophila chorion gene locus ACE3 replicator and ori-beta origin elements.

The following chif gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chif cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa14737	NM_001273570.2 Latest version!	Drosophila melanogaster chiffon (chif), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa14737	NM_165156.3 Latest version!	Drosophila melanogaster chiffon (chif), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa16833	NM_078859.4 Latest version!	Drosophila melanogaster chiffon (chif), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

chif ( NM_001273570.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260499.1 Drosophila melanogaster chiffon (chif), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa14737
Clone ID Related Accession (Same CDS sequence)	NM_001273570.2 , NM_165156.3
Accession Version	NM_001273570.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chiffon, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001273570.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAACCGC AGTCGGACAA ACAAAGCGCC AGCAGACTCG CAACAACAAC TAGCCACTCA 
ACAGCAGCAG CTTCCGCAAC AGCAGCAACT CCTCCTAAAG TAAAGGTCAT AAAGAGCAAA 
CGCCCCCTGT GCCACTTTAA GTTCTACCTG GACATTTGCG ATCATCAGTT AGCAAAGCGC 
ATTGAGTCCG ACATCAAAGC ATTGGGTGGA CATCTCGAGT TCTTTCTCAG TGACGATATA 
ACACATTTTG TCACTGATAA GCCAGAGGTC ATAGGCGGAA CCTCCGGAAC ACCAGGTACA 
CCTAGCACAC CCGGCACCCC CACGAGTCAC TACCAGCAAA ATGATGGTTC GGCAAGAAAA 
CCGAATCAGA GGCAATCAAG AGCGGATGCA ATACTGAGTC GCGTGCGGCG GAGCACGGTT 
GGAGTCGTAA ACAGTGGCAA TAGCACACCC ACGACATCCC TAAAACGGAG CTATACCATC 
TGGCAGACGG ATTATGCCCA ACGCTTTATA AAGCGCATTC AGACCGAGCT TAAGCAATAC 
CTTGAGGGTA AGAAGGAAGG TGGAGGAGGA TCAACATCAG CTAGCCCCCA TCATATACAG 
CTAAAAAAGC AGTATGTGAA GATCGAATCC GTCAAGCGTA ATTATAGACC TTACTACCAT 
CTTATCAAAC AGCCAGACGA CTGGCCAAAG ATAGATTTAA GCAGCGAAGA CGGCGCCTTT 
CGACTACTAA CAAAGTCGAA GACCAAGGAT AAGGAGCATA GCATGACCCG CAAGCCTTTG 
GGTTCACGCA CATCACAGAA AGACAAACAA GCTGCTGGGG AAGCAAAGCC ACTCCAGCAT 
CCTTCTCTGC AGGAACTTAA AAAACAGTCT GCGATTCCGA ATAGTCCGCG TTCCAATTGT 
CGCGAACCAA TAGATTCGAG TGAAAAACAA GGTGGAGTGT GTGAGATCTG TAAATTGGAA 
TACGATATCC TGAATATCCA TTTGCAGAGC AAAGATCACG AGCTGTTTGC AAAGAATTCG 
GATAATTTTC TGGCTCTAGA CACTCTAATT CAAAGTTCGG CGGATGTGAA TAGGTTCCTG 
GAGGAGGAGC CCGTAGAGAG TGAACTTGAC ATGGATGTCG ATGAATCGTT GAGTAATGAG 
GAGTTGCAGA GTCCCCGTCA GAGGCCATCG CCAGCGTTGC GGGAGAAGTC CAAAAGGATA 
ACCAAGGGAA AGCATTCGTC AGAAAAGTTT CAAGGAGTTG CAGTGGCATC ACCTCAAACG 
CCATTCCCTG GTGCCAAGAA GGTTCAAGGA AATTCACCTG GTAGTCTTTC TGAACTTCAG 
CGTCAGGAAC ATCCAACAAC AGCCGCAGCA ACGCCAACAA CGAATTCAGG ACGAAGGAAA 
ACACAGAACT CCGGTCTGTC ACCCCCTAAG AGAGCCATGT TGCCGCCTTC TTCTATTTAT 
AAAGTAGTAG AAACTAGGGA GGAATGTGCT ACGCCTCCCA GAGGGCGAGG AAGACCTCCC 
AACCAAGTGG ATTCGCCCTC GCTGATTGTA AAGTTCCAAA AGATCCGACA AACAGAGTTA 
CAACGACTCA ATGGAGAGGC AGAGAACTTT ATGTTTCCCA GAACAGCGGT GCCAACTACA 
AGGAGCAGCA GTGAGCTGCC CACGGATGTC GATCGACAAA CCACCTCGGA TGTAAGAGGC 
AGATACAGTA TCTCCTCCGC CAGCCTAGAC ACTAGCACCA GCGAAGCAGA AACTAAGGAA 
TCTTCAGGAT TACCTACTAG CATACGCAAG CGAGCTCAAG CAGTGGGAAG GCGAAGAAAA 
GTAGGAGGAG CAGCGGCGCA GGATGTTTTC CAACGCCAAT TATCAACGGG GAGTAGTAGC 
AGCAATAGTA ACCAGCAGCG CTTTCCAAGC GCCCCCATCC AGCCAGAAGA AGGGCCTCAA 
CCGCAGCCAA AACCGCAGCT AAAGATCAAG ATAAAGCAGG AGCAATTGGT GGCCACCAGG 
AAAAGCAGTC GCACAGCTAC GGCTATTGTG ACAGCAGCCA CCGCCAGCAG TCATCAGCAG 
CAGCAGCTAA GGCAGACAAC TTGCCGGAAA ATGGCCAATA AACTGGAGGA TAGGATGGGG 
GAATTAGTAA AGCCCAAAAT AAAAATAAAG AAGGAAGTGA TCGAAGAGCA GAAAGTGAAG 
GAGTTGGAAG ATCTGGAAGA GATATTAGAT AAAGAATTAG ATGAGGAGGT GGACAGTAGT 
TGCAGTAGTG GTAGCGATGA AGACTACATT GCTGGTAGCC AACGAAGAAT CACGGCAGCT 
CCTCGTAAAT CAACAGATAC TCGTGAACAA AGAGCAGCTA GGCGGCTTTC CAGATTAACC 
ATAAATCGTA GTGCCGGAGA GCTGGAGTTA ACGGAGGTCA AAACGTCTCC TTCGAAAAGC 
CGCACTAAGA TCCAGAAACC GTCAAGTCCC ACGAAAAATA AAGTTAAACA AACGAAGGCA 
GTGCCACCAG CGATAGATTT ATTCTTTGAT TGCAGTAAAA GCGAAAGACT TCGGGAAATG 
CAGTATACCT TCGAATCGTT GCCAAGTGGG GAGCTTTGGA ATAGAGTTTT TCTGCGACAG 
GATGCGGGTG AAGAGAACTA TTACACGTAT TATGGTAGCA CTAATTATAG GAAACTGCCC 
TATGAAATGG GTCCCATACC CATGGCCAAA ACATTGCCAG CACACAGTTG TGCATTATGC 
CGCGAGGCAA GCGAAGTAAA GCAGGATAAG GGAGAGCAGA TCAAGCTGGA AGATCAGAAG 
CCCGCGCCCA AGAAAGAAGT TAAGAAGGAG GAGGAAGTCC AGTCCTCTTC CTCATCCGCG 
ACCTACAAGA ACAAAAAGCT CCATCTGCTC CAACGTTATC AGCAGGAACA GGAGCAACTG 
CAGCAGTTGG AGGGAAATTC CTTGGCCACT GCCGGAGCCA AATGTGATAG CAAGGCGAGC 
ACACCAGAAT TACTGGAAAG GGAGTTTGCC TCGGGTTCTA TGGGGGATAG AGTGCAGCTG 
ATAGAGAGAG TGAGGAGCAC ATCCAGTTCA AGCTGCAGTA ACAGTCAACG GAGCGGCATA 
ACCTGCAGGA ATAAGCAGCT GGCCCGGATT GCCGAATTAC CACCAAGAAA ATCCCCTAGA 
GAACATGCTT CTACTCTGGC TTTGGTCAGT TGCATTATAC GGCAGCGTCA GGACTCACAA 
AGTAAAACGA ACTCAGAAGC AGAAGAGCCA CCTCCTCCTG TAGCCGCACC AAAGCTTAAA 
ACTCCGATAA AACAGGAACC AGTAGCACCT TCATCGCCCC GAACGACTCG TTCTCAAGCT 
GCGACTCCTG TTGAGGAACT ACGATTTGCC ACCGAGATTA GCGAGACAGT AAAGAGAATG 
AGGCGGGGGC AAAACAAATA TGATCATTCC CCGCCAGCGC CAGTTCCTAC CCCCGCAACT 
TCTTCACCCG TTAGAAGTCG CCGCCTGACA CCAGCGGCTC GTAATCAGAG TCAGATTTAT 
TCGCGCCGAT TGGAATTCGC AACCAGTCAA AGGGAATCCT CAGCAAGTGC GCTTCTGGGT 
AAGCGTAAAA GAAGAGTTAA TCCTTCCGTT GCAGGCACAG TGCGACCGAC TACCCAAAAT 
CTTCCCGGAA CGGGTGCGTA TCGTGGAGTC CGCAAATTGC CCAGCAAGAA GGGATTGCTC 
GAATACGAAA TGGAAACGTG TGCCCTTAAA GCTTTGGATC AAGCCCGTCA GTATTGCAAT 
CCTGGCTTCG TTGCCTGGCA GTTAGATAAA TATCTGGAGC TAGCTGGCAA GGAGTACGAT 
ATAGAATTTG ACCAGATATC ACCGGAGGTG GAGTCTGAAG GACGAGAAGA AAGACTTGTT 
AACACACCAC AAACTCCACC ACCTACTGAT TGCTTTACAA GCGAGTTTGA CTTGTGCGAT 
CTAATTATGG GTAGTGCAGG GAGTGGAGAT GATGATGAAG ATGTATCCAG AGGAAACCCA 
CCTGGTTCCG GTCGACGAAT GAGTAATCTC AATCTATACG CCAGCTATTA TAGGAAAAGG 
AAGAGCCTGA AGTCCAATCG CACGGGTTGG CCTAAAGCTC AGAGAAGACG GAACGCAGGC 
GGATTAGGTG GCAGTCGAGC TTTGCCAGAT GAGCGAATTA ATTTCCAGAA AATGGGTCTT 
GCTGAGCTAC ACCCTATAAA GCAGGAGCCA ATGGAAACGG AAGAGGAACA GACCACAACC 
ACTACCACAA CTACTACGAC TTCTGCGACG AGAGGAAATC TCCTGAGCAA AGACGATGAA 
GATGATGAAG GAGGCGGAAA CTCGCCCTCT GGTGGATCAC CTGCTGATGA TAAACAGAAT 
TCCCGCGAAG ATGCCGTGAT GACTCCGCCA GCGACCGATG TTGACGAGCA GGCTGAACCG 
CAGGCGGATG AAATGGAAAG TCTGCCCGAC GAAGATGAAA CAATGGCGGA TTCTGTGGAT 
CAGCAGCAGG ATGTTGAGGC GGAAATCGAA GCAACTGATG CGGATGTCGA GGAAGAAGAA 
GAGGAGGAGG ACGAGGACGA AGATGTTTTT GAAGATGCCT ACGAAGAGCA GGACATGGGC 
ATTCAGAAAA CTGAGCCTCA TGAAAAAAGA GCTCGAATAC CTTCCATATC GGTAACCACG 
CCGCCTGAAG ACTCTTCACA GGGCAAAAAA CTCTTGCTTA CTCTGCATAA TGGCCAGCGA 
CTACAGGCCA CCTCCACACC AAGTACCGGG CAAGTTCAAC AGCATCAGAG AAGAACTCCC 
CAGCTGAACG GAAGTCTAGG CAGCTGTATT TCGCCAAGTG AGAAACTGGG TGACAATTCC 
GACATATTCA CGGTATCATC TGACGGCTTG GATACCGATT TGGATTTAAG CAACACCCAG 
GCAGGCGATT CGCATGAGCA TTGCCCACAC CAGACGACGC CGAAGCGGAA GTTTGACATC 
TCCAAGTATG CGCCACCGAA TAGTGGAAAG GCGGCGAGCA GTTGTGCCGC CGAAGCGGCG 
ACGGCTGCCG TGAAGTCATT GGCCATCTCG CAGTTCCTGA AGAAGGAGGT GCGTGTCACC 
TGCAGGCGAC TAAGGGCGCC ATTCCGACGC TTTCGATACC GTCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260499.1
CDS	317..5404
Translation	MQPQSDKQSASRLATTTSHSTAAASATAATPPKVKVIKSKRPLCHFKFYLDICDHQLAKRIESDIKALGGHLEFFLSDDITHFVTDKPEVIGGTSGTPGTPSTPGTPTSHYQQNDGSARKPNQRQSRADAILSRVRRSTVGVVNSGNSTPTTSLKRSYTIWQTDYAQRFIKRIQTELKQYLEGKKEGGGGSTSASPHHIQLKKQYVKIESVKRNYRPYYHLIKQPDDWPKIDLSSEDGAFRLLTKSKTKDKEHSMTRKPLGSRTSQKDKQAAGEAKPLQHPSLQELKKQSAIPNSPRSNCREPIDSSEKQGGVCEICKLEYDILNIHLQSKDHELFAKNSDNFLALDTLIQSSADVNRFLEEEPVESELDMDVDESLSNEELQSPRQRPSPALREKSKRITKGKHSSEKFQGVAVASPQTPFPGAKKVQGNSPGSLSELQRQEHPTTAAATPTTNSGRRKTQNSGLSPPKRAMLPPSSIYKVVETREECATPPRGRGRPPNQVDSPSLIVKFQKIRQTELQRLNGEAENFMFPRTAVPTTRSSSELPTDVDRQTTSDVRGRYSISSASLDTSTSEAETKESSGLPTSIRKRAQAVGRRRKVGGAAAQDVFQRQLSTGSSSSNSNQQRFPSAPIQPEEGPQPQPKPQLKIKIKQEQLVATRKSSRTATAIVTAATASSHQQQQLRQTTCRKMANKLEDRMGELVKPKIKIKKEVIEEQKVKELEDLEEILDKELDEEVDSSCSSGSDEDYIAGSQRRITAAPRKSTDTREQRAARRLSRLTINRSAGELELTEVKTSPSKSRTKIQKPSSPTKNKVKQTKAVPPAIDLFFDCSKSERLREMQYTFESLPSGELWNRVFLRQDAGEENYYTYYGSTNYRKLPYEMGPIPMAKTLPAHSCALCREASEVKQDKGEQIKLEDQKPAPKKEVKKEEEVQSSSSSATYKNKKLHLLQRYQQEQEQLQQLEGNSLATAGAKCDSKASTPELLEREFASGSMGDRVQLIERVRSTSSSSCSNSQRSGITCRNKQLARIAELPPRKSPREHASTLALVSCIIRQRQDSQSKTNSEAEEPPPPVAAPKLKTPIKQEPVAPSSPRTTRSQAATPVEELRFATEISETVKRMRRGQNKYDHSPPAPVPTPATSSPVRSRRLTPAARNQSQIYSRRLEFATSQRESSASALLGKRKRRVNPSVAGTVRPTTQNLPGTGAYRGVRKLPSKKGLLEYEMETCALKALDQARQYCNPGFVAWQLDKYLELAGKEYDIEFDQISPEVESEGREERLVNTPQTPPPTDCFTSEFDLCDLIMGSAGSGDDDEDVSRGNPPGSGRRMSNLNLYASYYRKRKSLKSNRTGWPKAQRRRNAGGLGGSRALPDERINFQKMGLAELHPIKQEPMETEEEQTTTTTTTTTTSATRGNLLSKDDEDDEGGGNSPSGGSPADDKQNSREDAVMTPPATDVDEQAEPQADEMESLPDEDETMADSVDQQQDVEAEIEATDADVEEEEEEEDEDEDVFEDAYEEQDMGIQKTEPHEKRARIPSISVTTPPEDSSQGKKLLLTLHNGQRLQATSTPSTGQVQQHQRRTPQLNGSLGSCISPSEKLGDNSDIFTVSSDGLDTDLDLSNTQAGDSHEHCPHQTTPKRKFDISKYAPPNSGKAASSCAAEAATAAVKSLAISQFLKKEVRVTCRRLRAPFRRFRYRR

Target ORF information:

RefSeq Version	NM_001273570.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chiffon (chif), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273570.2

ORF Insert Sequence:

ATGCAACCGC AGTCGGACAA ACAAAGCGCC AGCAGACTCG CAACAACAAC TAGCCACTCA 
ACAGCAGCAG CTTCCGCAAC AGCAGCAACT CCTCCTAAAG TAAAGGTCAT AAAGAGCAAA 
CGCCCCCTGT GCCACTTTAA GTTCTACCTG GACATTTGCG ATCATCAGTT AGCAAAGCGC 
ATTGAGTCCG ACATCAAAGC ATTGGGTGGA CATCTCGAGT TCTTTCTCAG TGACGATATA 
ACACATTTTG TCACTGATAA GCCAGAGGTC ATAGGCGGAA CCTCCGGAAC ACCAGGTACA 
CCTAGCACAC CCGGCACCCC CACGAGTCAC TACCAGCAAA ATGATGGTTC GGCAAGAAAA 
CCGAATCAGA GGCAATCAAG AGCGGATGCA ATACTGAGTC GCGTGCGGCG GAGCACGGTT 
GGAGTCGTAA ACAGTGGCAA TAGCACACCC ACGACATCCC TAAAACGGAG CTATACCATC 
TGGCAGACGG ATTATGCCCA ACGCTTTATA AAGCGCATTC AGACCGAGCT TAAGCAATAC 
CTTGAGGGTA AGAAGGAAGG TGGAGGAGGA TCAACATCAG CTAGCCCCCA TCATATACAG 
CTAAAAAAGC AGTATGTGAA GATCGAATCC GTCAAGCGTA ATTATAGACC TTACTACCAT 
CTTATCAAAC AGCCAGACGA CTGGCCAAAG ATAGATTTAA GCAGCGAAGA CGGCGCCTTT 
CGACTACTAA CAAAGTCGAA GACCAAGGAT AAGGAGCATA GCATGACCCG CAAGCCTTTG 
GGTTCACGCA CATCACAGAA AGACAAACAA GCTGCTGGGG AAGCAAAGCC ACTCCAGCAT 
CCTTCTCTGC AGGAACTTAA AAAACAGTCT GCGATTCCGA ATAGTCCGCG TTCCAATTGT 
CGCGAACCAA TAGATTCGAG TGAAAAACAA GGTGGAGTGT GTGAGATCTG TAAATTGGAA 
TACGATATCC TGAATATCCA TTTGCAGAGC AAAGATCACG AGCTGTTTGC AAAGAATTCG 
GATAATTTTC TGGCTCTAGA CACTCTAATT CAAAGTTCGG CGGATGTGAA TAGGTTCCTG 
GAGGAGGAGC CCGTAGAGAG TGAACTTGAC ATGGATGTCG ATGAATCGTT GAGTAATGAG 
GAGTTGCAGA GTCCCCGTCA GAGGCCATCG CCAGCGTTGC GGGAGAAGTC CAAAAGGATA 
ACCAAGGGAA AGCATTCGTC AGAAAAGTTT CAAGGAGTTG CAGTGGCATC ACCTCAAACG 
CCATTCCCTG GTGCCAAGAA GGTTCAAGGA AATTCACCTG GTAGTCTTTC TGAACTTCAG 
CGTCAGGAAC ATCCAACAAC AGCCGCAGCA ACGCCAACAA CGAATTCAGG ACGAAGGAAA 
ACACAGAACT CCGGTCTGTC ACCCCCTAAG AGAGCCATGT TGCCGCCTTC TTCTATTTAT 
AAAGTAGTAG AAACTAGGGA GGAATGTGCT ACGCCTCCCA GAGGGCGAGG AAGACCTCCC 
AACCAAGTGG ATTCGCCCTC GCTGATTGTA AAGTTCCAAA AGATCCGACA AACAGAGTTA 
CAACGACTCA ATGGAGAGGC AGAGAACTTT ATGTTTCCCA GAACAGCGGT GCCAACTACA 
AGGAGCAGCA GTGAGCTGCC CACGGATGTC GATCGACAAA CCACCTCGGA TGTAAGAGGC 
AGATACAGTA TCTCCTCCGC CAGCCTAGAC ACTAGCACCA GCGAAGCAGA AACTAAGGAA 
TCTTCAGGAT TACCTACTAG CATACGCAAG CGAGCTCAAG CAGTGGGAAG GCGAAGAAAA 
GTAGGAGGAG CAGCGGCGCA GGATGTTTTC CAACGCCAAT TATCAACGGG GAGTAGTAGC 
AGCAATAGTA ACCAGCAGCG CTTTCCAAGC GCCCCCATCC AGCCAGAAGA AGGGCCTCAA 
CCGCAGCCAA AACCGCAGCT AAAGATCAAG ATAAAGCAGG AGCAATTGGT GGCCACCAGG 
AAAAGCAGTC GCACAGCTAC GGCTATTGTG ACAGCAGCCA CCGCCAGCAG TCATCAGCAG 
CAGCAGCTAA GGCAGACAAC TTGCCGGAAA ATGGCCAATA AACTGGAGGA TAGGATGGGG 
GAATTAGTAA AGCCCAAAAT AAAAATAAAG AAGGAAGTGA TCGAAGAGCA GAAAGTGAAG 
GAGTTGGAAG ATCTGGAAGA GATATTAGAT AAAGAATTAG ATGAGGAGGT GGACAGTAGT 
TGCAGTAGTG GTAGCGATGA AGACTACATT GCTGGTAGCC AACGAAGAAT CACGGCAGCT 
CCTCGTAAAT CAACAGATAC TCGTGAACAA AGAGCAGCTA GGCGGCTTTC CAGATTAACC 
ATAAATCGTA GTGCCGGAGA GCTGGAGTTA ACGGAGGTCA AAACGTCTCC TTCGAAAAGC 
CGCACTAAGA TCCAGAAACC GTCAAGTCCC ACGAAAAATA AAGTTAAACA AACGAAGGCA 
GTGCCACCAG CGATAGATTT ATTCTTTGAT TGCAGTAAAA GCGAAAGACT TCGGGAAATG 
CAGTATACCT TCGAATCGTT GCCAAGTGGG GAGCTTTGGA ATAGAGTTTT TCTGCGACAG 
GATGCGGGTG AAGAGAACTA TTACACGTAT TATGGTAGCA CTAATTATAG GAAACTGCCC 
TATGAAATGG GTCCCATACC CATGGCCAAA ACATTGCCAG CACACAGTTG TGCATTATGC 
CGCGAGGCAA GCGAAGTAAA GCAGGATAAG GGAGAGCAGA TCAAGCTGGA AGATCAGAAG 
CCCGCGCCCA AGAAAGAAGT TAAGAAGGAG GAGGAAGTCC AGTCCTCTTC CTCATCCGCG 
ACCTACAAGA ACAAAAAGCT CCATCTGCTC CAACGTTATC AGCAGGAACA GGAGCAACTG 
CAGCAGTTGG AGGGAAATTC CTTGGCCACT GCCGGAGCCA AATGTGATAG CAAGGCGAGC 
ACACCAGAAT TACTGGAAAG GGAGTTTGCC TCGGGTTCTA TGGGGGATAG AGTGCAGCTG 
ATAGAGAGAG TGAGGAGCAC ATCCAGTTCA AGCTGCAGTA ACAGTCAACG GAGCGGCATA 
ACCTGCAGGA ATAAGCAGCT GGCCCGGATT GCCGAATTAC CACCAAGAAA ATCCCCTAGA 
GAACATGCTT CTACTCTGGC TTTGGTCAGT TGCATTATAC GGCAGCGTCA GGACTCACAA 
AGTAAAACGA ACTCAGAAGC AGAAGAGCCA CCTCCTCCTG TAGCCGCACC AAAGCTTAAA 
ACTCCGATAA AACAGGAACC AGTAGCACCT TCATCGCCCC GAACGACTCG TTCTCAAGCT 
GCGACTCCTG TTGAGGAACT ACGATTTGCC ACCGAGATTA GCGAGACAGT AAAGAGAATG 
AGGCGGGGGC AAAACAAATA TGATCATTCC CCGCCAGCGC CAGTTCCTAC CCCCGCAACT 
TCTTCACCCG TTAGAAGTCG CCGCCTGACA CCAGCGGCTC GTAATCAGAG TCAGATTTAT 
TCGCGCCGAT TGGAATTCGC AACCAGTCAA AGGGAATCCT CAGCAAGTGC GCTTCTGGGT 
AAGCGTAAAA GAAGAGTTAA TCCTTCCGTT GCAGGCACAG TGCGACCGAC TACCCAAAAT 
CTTCCCGGAA CGGGTGCGTA TCGTGGAGTC CGCAAATTGC CCAGCAAGAA GGGATTGCTC 
GAATACGAAA TGGAAACGTG TGCCCTTAAA GCTTTGGATC AAGCCCGTCA GTATTGCAAT 
CCTGGCTTCG TTGCCTGGCA GTTAGATAAA TATCTGGAGC TAGCTGGCAA GGAGTACGAT 
ATAGAATTTG ACCAGATATC ACCGGAGGTG GAGTCTGAAG GACGAGAAGA AAGACTTGTT 
AACACACCAC AAACTCCACC ACCTACTGAT TGCTTTACAA GCGAGTTTGA CTTGTGCGAT 
CTAATTATGG GTAGTGCAGG GAGTGGAGAT GATGATGAAG ATGTATCCAG AGGAAACCCA 
CCTGGTTCCG GTCGACGAAT GAGTAATCTC AATCTATACG CCAGCTATTA TAGGAAAAGG 
AAGAGCCTGA AGTCCAATCG CACGGGTTGG CCTAAAGCTC AGAGAAGACG GAACGCAGGC 
GGATTAGGTG GCAGTCGAGC TTTGCCAGAT GAGCGAATTA ATTTCCAGAA AATGGGTCTT 
GCTGAGCTAC ACCCTATAAA GCAGGAGCCA ATGGAAACGG AAGAGGAACA GACCACAACC 
ACTACCACAA CTACTACGAC TTCTGCGACG AGAGGAAATC TCCTGAGCAA AGACGATGAA 
GATGATGAAG GAGGCGGAAA CTCGCCCTCT GGTGGATCAC CTGCTGATGA TAAACAGAAT 
TCCCGCGAAG ATGCCGTGAT GACTCCGCCA GCGACCGATG TTGACGAGCA GGCTGAACCG 
CAGGCGGATG AAATGGAAAG TCTGCCCGAC GAAGATGAAA CAATGGCGGA TTCTGTGGAT 
CAGCAGCAGG ATGTTGAGGC GGAAATCGAA GCAACTGATG CGGATGTCGA GGAAGAAGAA 
GAGGAGGAGG ACGAGGACGA AGATGTTTTT GAAGATGCCT ACGAAGAGCA GGACATGGGC 
ATTCAGAAAA CTGAGCCTCA TGAAAAAAGA GCTCGAATAC CTTCCATATC GGTAACCACG 
CCGCCTGAAG ACTCTTCACA GGGCAAAAAA CTCTTGCTTA CTCTGCATAA TGGCCAGCGA 
CTACAGGCCA CCTCCACACC AAGTACCGGG CAAGTTCAAC AGCATCAGAG AAGAACTCCC 
CAGCTGAACG GAAGTCTAGG CAGCTGTATT TCGCCAAGTG AGAAACTGGG TGACAATTCC 
GACATATTCA CGGTATCATC TGACGGCTTG GATACCGATT TGGATTTAAG CAACACCCAG 
GCAGGCGATT CGCATGAGCA TTGCCCACAC CAGACGACGC CGAAGCGGAA GTTTGACATC 
TCCAAGTATG CGCCACCGAA TAGTGGAAAG GCGGCGAGCA GTTGTGCCGC CGAAGCGGCG 
ACGGCTGCCG TGAAGTCATT GGCCATCTCG CAGTTCCTGA AGAAGGAGGT GCGTGTCACC 
TGCAGGCGAC TAAGGGCGCC ATTCCGACGC TTTCGATACC GTCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chif ( NM_165156.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723965.1 Drosophila melanogaster chiffon (chif), transcript variant B, mRNA.

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CloneID	OFa14737
Clone ID Related Accession (Same CDS sequence)	NM_001273570.2 , NM_165156.3
Accession Version	NM_165156.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chiffon, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165156.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAACCGC AGTCGGACAA ACAAAGCGCC AGCAGACTCG CAACAACAAC TAGCCACTCA 
ACAGCAGCAG CTTCCGCAAC AGCAGCAACT CCTCCTAAAG TAAAGGTCAT AAAGAGCAAA 
CGCCCCCTGT GCCACTTTAA GTTCTACCTG GACATTTGCG ATCATCAGTT AGCAAAGCGC 
ATTGAGTCCG ACATCAAAGC ATTGGGTGGA CATCTCGAGT TCTTTCTCAG TGACGATATA 
ACACATTTTG TCACTGATAA GCCAGAGGTC ATAGGCGGAA CCTCCGGAAC ACCAGGTACA 
CCTAGCACAC CCGGCACCCC CACGAGTCAC TACCAGCAAA ATGATGGTTC GGCAAGAAAA 
CCGAATCAGA GGCAATCAAG AGCGGATGCA ATACTGAGTC GCGTGCGGCG GAGCACGGTT 
GGAGTCGTAA ACAGTGGCAA TAGCACACCC ACGACATCCC TAAAACGGAG CTATACCATC 
TGGCAGACGG ATTATGCCCA ACGCTTTATA AAGCGCATTC AGACCGAGCT TAAGCAATAC 
CTTGAGGGTA AGAAGGAAGG TGGAGGAGGA TCAACATCAG CTAGCCCCCA TCATATACAG 
CTAAAAAAGC AGTATGTGAA GATCGAATCC GTCAAGCGTA ATTATAGACC TTACTACCAT 
CTTATCAAAC AGCCAGACGA CTGGCCAAAG ATAGATTTAA GCAGCGAAGA CGGCGCCTTT 
CGACTACTAA CAAAGTCGAA GACCAAGGAT AAGGAGCATA GCATGACCCG CAAGCCTTTG 
GGTTCACGCA CATCACAGAA AGACAAACAA GCTGCTGGGG AAGCAAAGCC ACTCCAGCAT 
CCTTCTCTGC AGGAACTTAA AAAACAGTCT GCGATTCCGA ATAGTCCGCG TTCCAATTGT 
CGCGAACCAA TAGATTCGAG TGAAAAACAA GGTGGAGTGT GTGAGATCTG TAAATTGGAA 
TACGATATCC TGAATATCCA TTTGCAGAGC AAAGATCACG AGCTGTTTGC AAAGAATTCG 
GATAATTTTC TGGCTCTAGA CACTCTAATT CAAAGTTCGG CGGATGTGAA TAGGTTCCTG 
GAGGAGGAGC CCGTAGAGAG TGAACTTGAC ATGGATGTCG ATGAATCGTT GAGTAATGAG 
GAGTTGCAGA GTCCCCGTCA GAGGCCATCG CCAGCGTTGC GGGAGAAGTC CAAAAGGATA 
ACCAAGGGAA AGCATTCGTC AGAAAAGTTT CAAGGAGTTG CAGTGGCATC ACCTCAAACG 
CCATTCCCTG GTGCCAAGAA GGTTCAAGGA AATTCACCTG GTAGTCTTTC TGAACTTCAG 
CGTCAGGAAC ATCCAACAAC AGCCGCAGCA ACGCCAACAA CGAATTCAGG ACGAAGGAAA 
ACACAGAACT CCGGTCTGTC ACCCCCTAAG AGAGCCATGT TGCCGCCTTC TTCTATTTAT 
AAAGTAGTAG AAACTAGGGA GGAATGTGCT ACGCCTCCCA GAGGGCGAGG AAGACCTCCC 
AACCAAGTGG ATTCGCCCTC GCTGATTGTA AAGTTCCAAA AGATCCGACA AACAGAGTTA 
CAACGACTCA ATGGAGAGGC AGAGAACTTT ATGTTTCCCA GAACAGCGGT GCCAACTACA 
AGGAGCAGCA GTGAGCTGCC CACGGATGTC GATCGACAAA CCACCTCGGA TGTAAGAGGC 
AGATACAGTA TCTCCTCCGC CAGCCTAGAC ACTAGCACCA GCGAAGCAGA AACTAAGGAA 
TCTTCAGGAT TACCTACTAG CATACGCAAG CGAGCTCAAG CAGTGGGAAG GCGAAGAAAA 
GTAGGAGGAG CAGCGGCGCA GGATGTTTTC CAACGCCAAT TATCAACGGG GAGTAGTAGC 
AGCAATAGTA ACCAGCAGCG CTTTCCAAGC GCCCCCATCC AGCCAGAAGA AGGGCCTCAA 
CCGCAGCCAA AACCGCAGCT AAAGATCAAG ATAAAGCAGG AGCAATTGGT GGCCACCAGG 
AAAAGCAGTC GCACAGCTAC GGCTATTGTG ACAGCAGCCA CCGCCAGCAG TCATCAGCAG 
CAGCAGCTAA GGCAGACAAC TTGCCGGAAA ATGGCCAATA AACTGGAGGA TAGGATGGGG 
GAATTAGTAA AGCCCAAAAT AAAAATAAAG AAGGAAGTGA TCGAAGAGCA GAAAGTGAAG 
GAGTTGGAAG ATCTGGAAGA GATATTAGAT AAAGAATTAG ATGAGGAGGT GGACAGTAGT 
TGCAGTAGTG GTAGCGATGA AGACTACATT GCTGGTAGCC AACGAAGAAT CACGGCAGCT 
CCTCGTAAAT CAACAGATAC TCGTGAACAA AGAGCAGCTA GGCGGCTTTC CAGATTAACC 
ATAAATCGTA GTGCCGGAGA GCTGGAGTTA ACGGAGGTCA AAACGTCTCC TTCGAAAAGC 
CGCACTAAGA TCCAGAAACC GTCAAGTCCC ACGAAAAATA AAGTTAAACA AACGAAGGCA 
GTGCCACCAG CGATAGATTT ATTCTTTGAT TGCAGTAAAA GCGAAAGACT TCGGGAAATG 
CAGTATACCT TCGAATCGTT GCCAAGTGGG GAGCTTTGGA ATAGAGTTTT TCTGCGACAG 
GATGCGGGTG AAGAGAACTA TTACACGTAT TATGGTAGCA CTAATTATAG GAAACTGCCC 
TATGAAATGG GTCCCATACC CATGGCCAAA ACATTGCCAG CACACAGTTG TGCATTATGC 
CGCGAGGCAA GCGAAGTAAA GCAGGATAAG GGAGAGCAGA TCAAGCTGGA AGATCAGAAG 
CCCGCGCCCA AGAAAGAAGT TAAGAAGGAG GAGGAAGTCC AGTCCTCTTC CTCATCCGCG 
ACCTACAAGA ACAAAAAGCT CCATCTGCTC CAACGTTATC AGCAGGAACA GGAGCAACTG 
CAGCAGTTGG AGGGAAATTC CTTGGCCACT GCCGGAGCCA AATGTGATAG CAAGGCGAGC 
ACACCAGAAT TACTGGAAAG GGAGTTTGCC TCGGGTTCTA TGGGGGATAG AGTGCAGCTG 
ATAGAGAGAG TGAGGAGCAC ATCCAGTTCA AGCTGCAGTA ACAGTCAACG GAGCGGCATA 
ACCTGCAGGA ATAAGCAGCT GGCCCGGATT GCCGAATTAC CACCAAGAAA ATCCCCTAGA 
GAACATGCTT CTACTCTGGC TTTGGTCAGT TGCATTATAC GGCAGCGTCA GGACTCACAA 
AGTAAAACGA ACTCAGAAGC AGAAGAGCCA CCTCCTCCTG TAGCCGCACC AAAGCTTAAA 
ACTCCGATAA AACAGGAACC AGTAGCACCT TCATCGCCCC GAACGACTCG TTCTCAAGCT 
GCGACTCCTG TTGAGGAACT ACGATTTGCC ACCGAGATTA GCGAGACAGT AAAGAGAATG 
AGGCGGGGGC AAAACAAATA TGATCATTCC CCGCCAGCGC CAGTTCCTAC CCCCGCAACT 
TCTTCACCCG TTAGAAGTCG CCGCCTGACA CCAGCGGCTC GTAATCAGAG TCAGATTTAT 
TCGCGCCGAT TGGAATTCGC AACCAGTCAA AGGGAATCCT CAGCAAGTGC GCTTCTGGGT 
AAGCGTAAAA GAAGAGTTAA TCCTTCCGTT GCAGGCACAG TGCGACCGAC TACCCAAAAT 
CTTCCCGGAA CGGGTGCGTA TCGTGGAGTC CGCAAATTGC CCAGCAAGAA GGGATTGCTC 
GAATACGAAA TGGAAACGTG TGCCCTTAAA GCTTTGGATC AAGCCCGTCA GTATTGCAAT 
CCTGGCTTCG TTGCCTGGCA GTTAGATAAA TATCTGGAGC TAGCTGGCAA GGAGTACGAT 
ATAGAATTTG ACCAGATATC ACCGGAGGTG GAGTCTGAAG GACGAGAAGA AAGACTTGTT 
AACACACCAC AAACTCCACC ACCTACTGAT TGCTTTACAA GCGAGTTTGA CTTGTGCGAT 
CTAATTATGG GTAGTGCAGG GAGTGGAGAT GATGATGAAG ATGTATCCAG AGGAAACCCA 
CCTGGTTCCG GTCGACGAAT GAGTAATCTC AATCTATACG CCAGCTATTA TAGGAAAAGG 
AAGAGCCTGA AGTCCAATCG CACGGGTTGG CCTAAAGCTC AGAGAAGACG GAACGCAGGC 
GGATTAGGTG GCAGTCGAGC TTTGCCAGAT GAGCGAATTA ATTTCCAGAA AATGGGTCTT 
GCTGAGCTAC ACCCTATAAA GCAGGAGCCA ATGGAAACGG AAGAGGAACA GACCACAACC 
ACTACCACAA CTACTACGAC TTCTGCGACG AGAGGAAATC TCCTGAGCAA AGACGATGAA 
GATGATGAAG GAGGCGGAAA CTCGCCCTCT GGTGGATCAC CTGCTGATGA TAAACAGAAT 
TCCCGCGAAG ATGCCGTGAT GACTCCGCCA GCGACCGATG TTGACGAGCA GGCTGAACCG 
CAGGCGGATG AAATGGAAAG TCTGCCCGAC GAAGATGAAA CAATGGCGGA TTCTGTGGAT 
CAGCAGCAGG ATGTTGAGGC GGAAATCGAA GCAACTGATG CGGATGTCGA GGAAGAAGAA 
GAGGAGGAGG ACGAGGACGA AGATGTTTTT GAAGATGCCT ACGAAGAGCA GGACATGGGC 
ATTCAGAAAA CTGAGCCTCA TGAAAAAAGA GCTCGAATAC CTTCCATATC GGTAACCACG 
CCGCCTGAAG ACTCTTCACA GGGCAAAAAA CTCTTGCTTA CTCTGCATAA TGGCCAGCGA 
CTACAGGCCA CCTCCACACC AAGTACCGGG CAAGTTCAAC AGCATCAGAG AAGAACTCCC 
CAGCTGAACG GAAGTCTAGG CAGCTGTATT TCGCCAAGTG AGAAACTGGG TGACAATTCC 
GACATATTCA CGGTATCATC TGACGGCTTG GATACCGATT TGGATTTAAG CAACACCCAG 
GCAGGCGATT CGCATGAGCA TTGCCCACAC CAGACGACGC CGAAGCGGAA GTTTGACATC 
TCCAAGTATG CGCCACCGAA TAGTGGAAAG GCGGCGAGCA GTTGTGCCGC CGAAGCGGCG 
ACGGCTGCCG TGAAGTCATT GGCCATCTCG CAGTTCCTGA AGAAGGAGGT GCGTGTCACC 
TGCAGGCGAC TAAGGGCGCC ATTCCGACGC TTTCGATACC GTCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723965.1
CDS	722..5809
Translation	MQPQSDKQSASRLATTTSHSTAAASATAATPPKVKVIKSKRPLCHFKFYLDICDHQLAKRIESDIKALGGHLEFFLSDDITHFVTDKPEVIGGTSGTPGTPSTPGTPTSHYQQNDGSARKPNQRQSRADAILSRVRRSTVGVVNSGNSTPTTSLKRSYTIWQTDYAQRFIKRIQTELKQYLEGKKEGGGGSTSASPHHIQLKKQYVKIESVKRNYRPYYHLIKQPDDWPKIDLSSEDGAFRLLTKSKTKDKEHSMTRKPLGSRTSQKDKQAAGEAKPLQHPSLQELKKQSAIPNSPRSNCREPIDSSEKQGGVCEICKLEYDILNIHLQSKDHELFAKNSDNFLALDTLIQSSADVNRFLEEEPVESELDMDVDESLSNEELQSPRQRPSPALREKSKRITKGKHSSEKFQGVAVASPQTPFPGAKKVQGNSPGSLSELQRQEHPTTAAATPTTNSGRRKTQNSGLSPPKRAMLPPSSIYKVVETREECATPPRGRGRPPNQVDSPSLIVKFQKIRQTELQRLNGEAENFMFPRTAVPTTRSSSELPTDVDRQTTSDVRGRYSISSASLDTSTSEAETKESSGLPTSIRKRAQAVGRRRKVGGAAAQDVFQRQLSTGSSSSNSNQQRFPSAPIQPEEGPQPQPKPQLKIKIKQEQLVATRKSSRTATAIVTAATASSHQQQQLRQTTCRKMANKLEDRMGELVKPKIKIKKEVIEEQKVKELEDLEEILDKELDEEVDSSCSSGSDEDYIAGSQRRITAAPRKSTDTREQRAARRLSRLTINRSAGELELTEVKTSPSKSRTKIQKPSSPTKNKVKQTKAVPPAIDLFFDCSKSERLREMQYTFESLPSGELWNRVFLRQDAGEENYYTYYGSTNYRKLPYEMGPIPMAKTLPAHSCALCREASEVKQDKGEQIKLEDQKPAPKKEVKKEEEVQSSSSSATYKNKKLHLLQRYQQEQEQLQQLEGNSLATAGAKCDSKASTPELLEREFASGSMGDRVQLIERVRSTSSSSCSNSQRSGITCRNKQLARIAELPPRKSPREHASTLALVSCIIRQRQDSQSKTNSEAEEPPPPVAAPKLKTPIKQEPVAPSSPRTTRSQAATPVEELRFATEISETVKRMRRGQNKYDHSPPAPVPTPATSSPVRSRRLTPAARNQSQIYSRRLEFATSQRESSASALLGKRKRRVNPSVAGTVRPTTQNLPGTGAYRGVRKLPSKKGLLEYEMETCALKALDQARQYCNPGFVAWQLDKYLELAGKEYDIEFDQISPEVESEGREERLVNTPQTPPPTDCFTSEFDLCDLIMGSAGSGDDDEDVSRGNPPGSGRRMSNLNLYASYYRKRKSLKSNRTGWPKAQRRRNAGGLGGSRALPDERINFQKMGLAELHPIKQEPMETEEEQTTTTTTTTTTSATRGNLLSKDDEDDEGGGNSPSGGSPADDKQNSREDAVMTPPATDVDEQAEPQADEMESLPDEDETMADSVDQQQDVEAEIEATDADVEEEEEEEDEDEDVFEDAYEEQDMGIQKTEPHEKRARIPSISVTTPPEDSSQGKKLLLTLHNGQRLQATSTPSTGQVQQHQRRTPQLNGSLGSCISPSEKLGDNSDIFTVSSDGLDTDLDLSNTQAGDSHEHCPHQTTPKRKFDISKYAPPNSGKAASSCAAEAATAAVKSLAISQFLKKEVRVTCRRLRAPFRRFRYRR

Target ORF information:

RefSeq Version	NM_165156.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chiffon (chif), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165156.3

ORF Insert Sequence:

ATGCAACCGC AGTCGGACAA ACAAAGCGCC AGCAGACTCG CAACAACAAC TAGCCACTCA 
ACAGCAGCAG CTTCCGCAAC AGCAGCAACT CCTCCTAAAG TAAAGGTCAT AAAGAGCAAA 
CGCCCCCTGT GCCACTTTAA GTTCTACCTG GACATTTGCG ATCATCAGTT AGCAAAGCGC 
ATTGAGTCCG ACATCAAAGC ATTGGGTGGA CATCTCGAGT TCTTTCTCAG TGACGATATA 
ACACATTTTG TCACTGATAA GCCAGAGGTC ATAGGCGGAA CCTCCGGAAC ACCAGGTACA 
CCTAGCACAC CCGGCACCCC CACGAGTCAC TACCAGCAAA ATGATGGTTC GGCAAGAAAA 
CCGAATCAGA GGCAATCAAG AGCGGATGCA ATACTGAGTC GCGTGCGGCG GAGCACGGTT 
GGAGTCGTAA ACAGTGGCAA TAGCACACCC ACGACATCCC TAAAACGGAG CTATACCATC 
TGGCAGACGG ATTATGCCCA ACGCTTTATA AAGCGCATTC AGACCGAGCT TAAGCAATAC 
CTTGAGGGTA AGAAGGAAGG TGGAGGAGGA TCAACATCAG CTAGCCCCCA TCATATACAG 
CTAAAAAAGC AGTATGTGAA GATCGAATCC GTCAAGCGTA ATTATAGACC TTACTACCAT 
CTTATCAAAC AGCCAGACGA CTGGCCAAAG ATAGATTTAA GCAGCGAAGA CGGCGCCTTT 
CGACTACTAA CAAAGTCGAA GACCAAGGAT AAGGAGCATA GCATGACCCG CAAGCCTTTG 
GGTTCACGCA CATCACAGAA AGACAAACAA GCTGCTGGGG AAGCAAAGCC ACTCCAGCAT 
CCTTCTCTGC AGGAACTTAA AAAACAGTCT GCGATTCCGA ATAGTCCGCG TTCCAATTGT 
CGCGAACCAA TAGATTCGAG TGAAAAACAA GGTGGAGTGT GTGAGATCTG TAAATTGGAA 
TACGATATCC TGAATATCCA TTTGCAGAGC AAAGATCACG AGCTGTTTGC AAAGAATTCG 
GATAATTTTC TGGCTCTAGA CACTCTAATT CAAAGTTCGG CGGATGTGAA TAGGTTCCTG 
GAGGAGGAGC CCGTAGAGAG TGAACTTGAC ATGGATGTCG ATGAATCGTT GAGTAATGAG 
GAGTTGCAGA GTCCCCGTCA GAGGCCATCG CCAGCGTTGC GGGAGAAGTC CAAAAGGATA 
ACCAAGGGAA AGCATTCGTC AGAAAAGTTT CAAGGAGTTG CAGTGGCATC ACCTCAAACG 
CCATTCCCTG GTGCCAAGAA GGTTCAAGGA AATTCACCTG GTAGTCTTTC TGAACTTCAG 
CGTCAGGAAC ATCCAACAAC AGCCGCAGCA ACGCCAACAA CGAATTCAGG ACGAAGGAAA 
ACACAGAACT CCGGTCTGTC ACCCCCTAAG AGAGCCATGT TGCCGCCTTC TTCTATTTAT 
AAAGTAGTAG AAACTAGGGA GGAATGTGCT ACGCCTCCCA GAGGGCGAGG AAGACCTCCC 
AACCAAGTGG ATTCGCCCTC GCTGATTGTA AAGTTCCAAA AGATCCGACA AACAGAGTTA 
CAACGACTCA ATGGAGAGGC AGAGAACTTT ATGTTTCCCA GAACAGCGGT GCCAACTACA 
AGGAGCAGCA GTGAGCTGCC CACGGATGTC GATCGACAAA CCACCTCGGA TGTAAGAGGC 
AGATACAGTA TCTCCTCCGC CAGCCTAGAC ACTAGCACCA GCGAAGCAGA AACTAAGGAA 
TCTTCAGGAT TACCTACTAG CATACGCAAG CGAGCTCAAG CAGTGGGAAG GCGAAGAAAA 
GTAGGAGGAG CAGCGGCGCA GGATGTTTTC CAACGCCAAT TATCAACGGG GAGTAGTAGC 
AGCAATAGTA ACCAGCAGCG CTTTCCAAGC GCCCCCATCC AGCCAGAAGA AGGGCCTCAA 
CCGCAGCCAA AACCGCAGCT AAAGATCAAG ATAAAGCAGG AGCAATTGGT GGCCACCAGG 
AAAAGCAGTC GCACAGCTAC GGCTATTGTG ACAGCAGCCA CCGCCAGCAG TCATCAGCAG 
CAGCAGCTAA GGCAGACAAC TTGCCGGAAA ATGGCCAATA AACTGGAGGA TAGGATGGGG 
GAATTAGTAA AGCCCAAAAT AAAAATAAAG AAGGAAGTGA TCGAAGAGCA GAAAGTGAAG 
GAGTTGGAAG ATCTGGAAGA GATATTAGAT AAAGAATTAG ATGAGGAGGT GGACAGTAGT 
TGCAGTAGTG GTAGCGATGA AGACTACATT GCTGGTAGCC AACGAAGAAT CACGGCAGCT 
CCTCGTAAAT CAACAGATAC TCGTGAACAA AGAGCAGCTA GGCGGCTTTC CAGATTAACC 
ATAAATCGTA GTGCCGGAGA GCTGGAGTTA ACGGAGGTCA AAACGTCTCC TTCGAAAAGC 
CGCACTAAGA TCCAGAAACC GTCAAGTCCC ACGAAAAATA AAGTTAAACA AACGAAGGCA 
GTGCCACCAG CGATAGATTT ATTCTTTGAT TGCAGTAAAA GCGAAAGACT TCGGGAAATG 
CAGTATACCT TCGAATCGTT GCCAAGTGGG GAGCTTTGGA ATAGAGTTTT TCTGCGACAG 
GATGCGGGTG AAGAGAACTA TTACACGTAT TATGGTAGCA CTAATTATAG GAAACTGCCC 
TATGAAATGG GTCCCATACC CATGGCCAAA ACATTGCCAG CACACAGTTG TGCATTATGC 
CGCGAGGCAA GCGAAGTAAA GCAGGATAAG GGAGAGCAGA TCAAGCTGGA AGATCAGAAG 
CCCGCGCCCA AGAAAGAAGT TAAGAAGGAG GAGGAAGTCC AGTCCTCTTC CTCATCCGCG 
ACCTACAAGA ACAAAAAGCT CCATCTGCTC CAACGTTATC AGCAGGAACA GGAGCAACTG 
CAGCAGTTGG AGGGAAATTC CTTGGCCACT GCCGGAGCCA AATGTGATAG CAAGGCGAGC 
ACACCAGAAT TACTGGAAAG GGAGTTTGCC TCGGGTTCTA TGGGGGATAG AGTGCAGCTG 
ATAGAGAGAG TGAGGAGCAC ATCCAGTTCA AGCTGCAGTA ACAGTCAACG GAGCGGCATA 
ACCTGCAGGA ATAAGCAGCT GGCCCGGATT GCCGAATTAC CACCAAGAAA ATCCCCTAGA 
GAACATGCTT CTACTCTGGC TTTGGTCAGT TGCATTATAC GGCAGCGTCA GGACTCACAA 
AGTAAAACGA ACTCAGAAGC AGAAGAGCCA CCTCCTCCTG TAGCCGCACC AAAGCTTAAA 
ACTCCGATAA AACAGGAACC AGTAGCACCT TCATCGCCCC GAACGACTCG TTCTCAAGCT 
GCGACTCCTG TTGAGGAACT ACGATTTGCC ACCGAGATTA GCGAGACAGT AAAGAGAATG 
AGGCGGGGGC AAAACAAATA TGATCATTCC CCGCCAGCGC CAGTTCCTAC CCCCGCAACT 
TCTTCACCCG TTAGAAGTCG CCGCCTGACA CCAGCGGCTC GTAATCAGAG TCAGATTTAT 
TCGCGCCGAT TGGAATTCGC AACCAGTCAA AGGGAATCCT CAGCAAGTGC GCTTCTGGGT 
AAGCGTAAAA GAAGAGTTAA TCCTTCCGTT GCAGGCACAG TGCGACCGAC TACCCAAAAT 
CTTCCCGGAA CGGGTGCGTA TCGTGGAGTC CGCAAATTGC CCAGCAAGAA GGGATTGCTC 
GAATACGAAA TGGAAACGTG TGCCCTTAAA GCTTTGGATC AAGCCCGTCA GTATTGCAAT 
CCTGGCTTCG TTGCCTGGCA GTTAGATAAA TATCTGGAGC TAGCTGGCAA GGAGTACGAT 
ATAGAATTTG ACCAGATATC ACCGGAGGTG GAGTCTGAAG GACGAGAAGA AAGACTTGTT 
AACACACCAC AAACTCCACC ACCTACTGAT TGCTTTACAA GCGAGTTTGA CTTGTGCGAT 
CTAATTATGG GTAGTGCAGG GAGTGGAGAT GATGATGAAG ATGTATCCAG AGGAAACCCA 
CCTGGTTCCG GTCGACGAAT GAGTAATCTC AATCTATACG CCAGCTATTA TAGGAAAAGG 
AAGAGCCTGA AGTCCAATCG CACGGGTTGG CCTAAAGCTC AGAGAAGACG GAACGCAGGC 
GGATTAGGTG GCAGTCGAGC TTTGCCAGAT GAGCGAATTA ATTTCCAGAA AATGGGTCTT 
GCTGAGCTAC ACCCTATAAA GCAGGAGCCA ATGGAAACGG AAGAGGAACA GACCACAACC 
ACTACCACAA CTACTACGAC TTCTGCGACG AGAGGAAATC TCCTGAGCAA AGACGATGAA 
GATGATGAAG GAGGCGGAAA CTCGCCCTCT GGTGGATCAC CTGCTGATGA TAAACAGAAT 
TCCCGCGAAG ATGCCGTGAT GACTCCGCCA GCGACCGATG TTGACGAGCA GGCTGAACCG 
CAGGCGGATG AAATGGAAAG TCTGCCCGAC GAAGATGAAA CAATGGCGGA TTCTGTGGAT 
CAGCAGCAGG ATGTTGAGGC GGAAATCGAA GCAACTGATG CGGATGTCGA GGAAGAAGAA 
GAGGAGGAGG ACGAGGACGA AGATGTTTTT GAAGATGCCT ACGAAGAGCA GGACATGGGC 
ATTCAGAAAA CTGAGCCTCA TGAAAAAAGA GCTCGAATAC CTTCCATATC GGTAACCACG 
CCGCCTGAAG ACTCTTCACA GGGCAAAAAA CTCTTGCTTA CTCTGCATAA TGGCCAGCGA 
CTACAGGCCA CCTCCACACC AAGTACCGGG CAAGTTCAAC AGCATCAGAG AAGAACTCCC 
CAGCTGAACG GAAGTCTAGG CAGCTGTATT TCGCCAAGTG AGAAACTGGG TGACAATTCC 
GACATATTCA CGGTATCATC TGACGGCTTG GATACCGATT TGGATTTAAG CAACACCCAG 
GCAGGCGATT CGCATGAGCA TTGCCCACAC CAGACGACGC CGAAGCGGAA GTTTGACATC 
TCCAAGTATG CGCCACCGAA TAGTGGAAAG GCGGCGAGCA GTTGTGCCGC CGAAGCGGCG 
ACGGCTGCCG TGAAGTCATT GGCCATCTCG CAGTTCCTGA AGAAGGAGGT GCGTGTCACC 
TGCAGGCGAC TAAGGGCGCC ATTCCGACGC TTTCGATACC GTCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chif ( NM_078859.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523583.2 Drosophila melanogaster chiffon (chif), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa16833
Clone ID Related Accession (Same CDS sequence)	NM_078859.4
Accession Version	NM_078859.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chiffon, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_078859.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAACCGC AGTCGGACAA ACAAAGCGCC AGCAGACTCG CAACAACAAC TAGCCACTCA 
ACAGCAGCAG CTTCCGCAAC AGCAGCAACT CCTCCTAAAG TAAAGGTCAT AAAGAGCAAA 
CGCCCCCTGT GCCACTTTAA GTTCTACCTG GACATTTGCG ATCATCAGTT AGCAAAGCGC 
ATTGAGTCCG ACATCAAAGC ATTGGGTGGA CATCTCGAGT TCTTTCTCAG TGACGATATA 
ACACATTTTG TCACTGATAA GCCAGAGGTC ATAGGCGGAA CCTCCGGAAC ACCAGGTACA 
CCTAGCACAC CCGGCACCCC CACGAGTCAC TACCAGCAAA ATGATGGTTC GGCAAGAAAA 
CCGAATCAGA GGCAATCAAG AGCGGATGCA ATACTGAGTC GCGTGCGGCG GAGCACGGTT 
GGAGTCGTAA ACAGTGGCAA TAGCACACCC ACGACATCCC TAAAACGGAG CTATACCATC 
TGGCAGACGG ATTATGCCCA ACGCTTTATA AAGCGCATTC AGACCGAGCT TAAGCAATAC 
CTTGAGGGTA AGAAGGAAGG TGGAGGAGGA TCAACATCAG CTAGCCCCCA TCATATACAG 
CTAAAAAAGC AGTATGTGAA GATCGAATCC GTCAAGCGTA ATTATAGACC TTACTACCAT 
CTTATCAAAC AGCCAGACGA CTGGCCAAAG ATAGATTTAA GCAGCGAAGA CGGCGCCTTT 
CGACTACTAA CAAAGTCGAA GACCAAGGAT AAGGAGCATA GCATGACCCG CAAGCCTTTG 
GGTTCACGCA CATCACAGAA AGACAAACAA GCTGCTGGGG AAGCAAAGCC ACTCCAGCAT 
CCTTCTCTGC AGGAACTTAA AAAACAGTCT GCGATTCCGA ATAGTCCGCG TTCCAATTGT 
CGCGAACCAA TAGATTCGAG TGAAAAACAA GGTGGAGTGT GTGAGATCTG TAAATTGGAA 
TACGATATCC TGAATATCCA TTTGCAGAGC AAAGATCACG AGCTGTTTGC AAAGAATTCG 
GATAATTTTC TGGCTCTAGA CACTCTAATT CAAAGTTCGG CGGATGTGAA TAGGTTCCTG 
GAGGAGGAGC CCGTAGAGAG TGAACTTGAC ATGGATGTCG ATGAATCGTT GAGTAATGAG 
GAGTTGCAGA GTCCCCGTCA GAGGCCATCG CCAGCGTTGC GGGAGAAGTC CAAAAGGATA 
ACCAAGGGAA AGCATTCGTC AGAAAAGTTT CAAGGAGTTG CAGTGGCATC ACCTCAAACG 
CCATTCCCTG GTGCCAAGAA GGTTCAAGGA AATTCACCTG GTAGTCTTTC TGAACTTCAG 
CGTCAGGAAC ATCCAACAAC AGCCGCAGCA ACGCCAACAA CGAATTCAGG ACGAAGGAAA 
ACACAGAACT CCGGTCTGTC ACCCCCTAAG AGAGCCATGT TGCCGCCTTC TTCTATTTAT 
AAAGTAGTAG AAACTAGGGA GGAATGTGCT ACGCCTCCCA GAGGGCGAGG AAGACCTCCC 
AACCAAGTGG ATTCGCCCTC GCTGATTGTA AAGTTCCAAA AGATCCGACA AACAGAGTTA 
CAACGACTCA ATGGAGAGGC AGAGAACTTT ATGTTTCCCA GAACAGCGGT GCCAACTACA 
AGGAGCAGCA GTGAGCTGCC CACGGATGTC GATCGACAAA CCACCTCGGA TGTAAGAGGC 
AGATACAGTA TCTCCTCCGC CAGCCTAGAC ACTAGCACCA GCGAAGCAGA AACTAAGGAA 
TCTTCAGGAT TACCTACTAG CATACGCAAG CGAGCTCAAG CAGTGGGAAG GCGAAGAAAA 
GTAGGAGGAG CAGCGGCGCA GGATGTTTTC CAACGCCAAT TATCAACGGG GAGTAGTAGC 
AGCAATAGTA ACCAGCAGCG CTTTCCAAGC GCCCCCATCC AGCCAGAAGA AGGGCCTCAA 
CCGCAGCCAA AACCGCAGCT AAAGATCAAG ATAAAGCAGG AGCAATTGGT GGCCACCAGG 
AAAAGCAGTC GCACAGCTAC GGCTATTGTG ACAGCAGCCA CCGCCAGCAG TCATCAGCAG 
CAGCAGCTAA GGCAGACAAC TTGCCGGAAA ATGGCCAATA AACTGGAGGA TAGGATGGGG 
GAATTAGTAA AGCCCAAAAT AAAAATAAAG AAGGAAGTGA TCGAAGAGCA GAAAGTGAAG 
GAGTTGGAAG ATCTGGAAGA GATATTAGAT AAAGAATTAG ATGAGGAGGT GGACAGTAGT 
TGCAGTAGTG GTAGCGATGA AGACTACATT GCTGGTAGCC AACGAAGAAT CACGGCAGCT 
CCTCGTAAAT CAACAGATAC TCGTGAACAA AGAGCAGCTA GGCGGCTTTC CAGATTAACC 
ATAAATCGTA GTGCCGGAGA GCTGGAGTTA ACGGAGGTCA AAACGTCTCC TTCGAAAAGC 
CGCACTAAGA TCCAGAAACC GTCAAGTCCC ACGAAAAATA AAGTTAAACA AACGAAGGCA 
GTGCCACCAG CGATAGATTT ATTCTTTGAT TGCAGTAAAA GCGAAAGACT TCGGGAAATG 
CAGTATACCT TCGAATCGTT GCCAAGTGGG GAGCTTTGGA ATAGAGTTTT TCTGCGACAG 
GATGCGGGTG AAGAGAACTA TTACACGTAT TATGGTAGCA CTAATTATAG GAAACTGCCC 
TATGAAATGG GTCCCATACC CATGGCCAAA ACATTGCCAG CACACAGTTG TGCATTATGC 
CGCGAGGCAA GCGAAGTAAA GCAGGATAAG GGAGAGCAGA TCAAGCTGGA AGATCAGAAG 
CCCGCGCCCA AGAAAGAAGT TAAGAAGGAG GAGGAAGTCC AGTCCTCTTC CTCATCCGCG 
ACCTACAAGA ACAAAAAGCT CCATCTGCTC CAACGTTATC AGCAGGAACA GGAGCAACTG 
CAGCAGTTGG AGGGAAATTC CTTGGCCACT GCCGGAGCCA AATGTGATAG CAAGGCGAGC 
ACACCAGAAT TACTGGAAAG GGAGTTTGCC TCGGGTTCTA TGGGGGATAG AGTGCAGCTG 
ATAGAGAGAG TGAGGAGCAC ATCCAGTTCA AGCTGCAGTA ACAGTCAACG GAGCGGCATA 
ACCTGCAGGA ATAAGCAGCT GGCCCGGATT GCCGAATTAC CACCAAGAAA ATCCCCTAGA 
GAACATGCTT CTACTCTGGC TTTGGTCAGT TGCATTATAC GGCAGCGTCA GGACTCACAA 
AGTAAAACGA ACTCAGAAGC AGAAGAGCCA CCTCCTCCTG TAGCCGCACC AAAGCTTAAA 
ACTCCGATAA AACAGGAACC AGTAGCACCT TCATCGCCCC GAACGACTCG TTCTCAAGCT 
GCGACTCCTG TTGAGGAACT ACGATTTGCC ACCGAGATTA GCGAGACAGT AAAGAGAATG 
AGGCGGGGGC AAAACAAATA TGATCATTCC CCGCCAGCGC CAGTTCCTAC CCCCGCAACT 
TCTTCACCCG TTAGAAGTCG CCGCCTGACA CCAGCGGCTC GTAATCAGAG TCAGATTTAT 
TCGCGCCGAT TGGAATTCGC AACCAGTCAA AGGGAATCCT CAGCAAGTGC GCTTCTGGGT 
AAGCGTAAAA GAAGAGTTAA TCCTTCCGTT GCAGGCACAG TGCGACCGAC TACCCAAAAT 
CTTCCCGGAA CGGGTGCGTA TCGTGGAGTC CGCAAATTGC CCAGCAAGAA GGGATTGCTC 
GAATACGAAA TGGAAACGTG TGCCCTTAAA GCTTTGGATC AAGCCCGTCA GTATTGCAAT 
CCTGGCTTCG TTGCCTGGCA GTTAGATAAA TATCTGGAGC TAGCTGGCAA GGAGTACGAT 
ATAGAATTTG ACCAGATATC ACCGGAGGTG GAGTCTGAAG GACGAGAAGA AAGACTTGTT 
AACACACCAC AAACTCCACC ACCTACTGAT TGCTTTACAA GCGAGTTTGA CTTGTGCGAT 
CTAATTATGG GTAGTGCAGG GAGTGGAGAT GATGATGAAG ATGTATCCAG AGGAAACCCA 
CCTGGTTCCG GTCGACGAAT GAGTAATCTC AATCTATACG CCAGCTATTA TAGGAAAAGG 
AAGAGCCTGA AGTCCAATCG CACGGGTTGG CCTAAAGCTC AGAGAAGACG GAACGCAGGC 
GGATTAGGTG GCAGTCGAGC TTTGCCAGAT GAGCGAATTA ATTTCCAGAA AATGGGTCTT 
GCTGAGCTAC ACCCTATAAA GCAGGAGCCA ATGGAAACGG AAGAGGAACA GACCACAACC 
ACTACCACAA CTACTACGAC TTCTGCGACG AGAGGAAATC TCCTGAGCAA AGACGATGAA 
GATGATGAAG GAGGCGGAAA CTCGCCCTCT GGTGGATCAC CTGCTGATGA TAAACAGAAT 
TCCCGCGAAG ATGCCGTGAT GACTCCGCCA GCGACCGATG TTGACGAGCA GGCTGAACCG 
CAGGCGGATG AAATGGAAAG TCTGCCCGAC GAAGATGAAA CAATGGCGGA TTCTGTGGAT 
CAGCAGCAGG ATGTTGAGGC GGAAATCGAA GCAACTGATG CGGATGTCGA GGAAGAAGAA 
GAGGAGGAGG ACGAGGACGA AGATGTTTTT GAAGATGCCT ACGAAGAGCA GGACATGGGC 
ATTCAGAAAA CTGAGCCTCA TGAAAAAAGA GCTCGAATAC CTTCCATATC GGTAACCACG 
CCGCCTGAAG ACTCTTCACA GGGCAAAAAA CTCTTGCTTA CTCTGCATAA TGGCCAGCGA 
CTACAGGCCA CCTCCACACC AAGTACCGGG CAAGTTCAAC AGCATCAGAG AAGAACTCCC 
CAGCTGAACG GAAGTCTAGG CAGCTGTATT TCGCCAAGTG AGAAACTGGG TGACAATTCC 
GACATATTCA CGGTATCATC TGACGGCTTG GATACCGATT TGGATTTAAG CAACACCCAG 
GCAGGCGATT CGCATGAGCA TTGCCCACAC CAGACGACGC CGAAGCGGAA GTTTGACATC 
TCCAAGTATG CGCCACCGAA TAGTGGAAAG GCGGCGAGCA GTTGTGCCGC CGAAGCGGCG 
ACGGCTGCCG TGAAGTCATT GGCCATCTCG CAGTTCCTGA AGAAGGAGAC TTGCGCAAGC 
TCATCAAGCA TGAGGAACGC CTGGCGGAGA ACGCAAAGGA GAGCGATTTC AGCGGCCTGC 
ATAAGCGCGG CACCGTCAGC TCGCGTGCCA AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523583.2
CDS	722..5857
Translation	MQPQSDKQSASRLATTTSHSTAAASATAATPPKVKVIKSKRPLCHFKFYLDICDHQLAKRIESDIKALGGHLEFFLSDDITHFVTDKPEVIGGTSGTPGTPSTPGTPTSHYQQNDGSARKPNQRQSRADAILSRVRRSTVGVVNSGNSTPTTSLKRSYTIWQTDYAQRFIKRIQTELKQYLEGKKEGGGGSTSASPHHIQLKKQYVKIESVKRNYRPYYHLIKQPDDWPKIDLSSEDGAFRLLTKSKTKDKEHSMTRKPLGSRTSQKDKQAAGEAKPLQHPSLQELKKQSAIPNSPRSNCREPIDSSEKQGGVCEICKLEYDILNIHLQSKDHELFAKNSDNFLALDTLIQSSADVNRFLEEEPVESELDMDVDESLSNEELQSPRQRPSPALREKSKRITKGKHSSEKFQGVAVASPQTPFPGAKKVQGNSPGSLSELQRQEHPTTAAATPTTNSGRRKTQNSGLSPPKRAMLPPSSIYKVVETREECATPPRGRGRPPNQVDSPSLIVKFQKIRQTELQRLNGEAENFMFPRTAVPTTRSSSELPTDVDRQTTSDVRGRYSISSASLDTSTSEAETKESSGLPTSIRKRAQAVGRRRKVGGAAAQDVFQRQLSTGSSSSNSNQQRFPSAPIQPEEGPQPQPKPQLKIKIKQEQLVATRKSSRTATAIVTAATASSHQQQQLRQTTCRKMANKLEDRMGELVKPKIKIKKEVIEEQKVKELEDLEEILDKELDEEVDSSCSSGSDEDYIAGSQRRITAAPRKSTDTREQRAARRLSRLTINRSAGELELTEVKTSPSKSRTKIQKPSSPTKNKVKQTKAVPPAIDLFFDCSKSERLREMQYTFESLPSGELWNRVFLRQDAGEENYYTYYGSTNYRKLPYEMGPIPMAKTLPAHSCALCREASEVKQDKGEQIKLEDQKPAPKKEVKKEEEVQSSSSSATYKNKKLHLLQRYQQEQEQLQQLEGNSLATAGAKCDSKASTPELLEREFASGSMGDRVQLIERVRSTSSSSCSNSQRSGITCRNKQLARIAELPPRKSPREHASTLALVSCIIRQRQDSQSKTNSEAEEPPPPVAAPKLKTPIKQEPVAPSSPRTTRSQAATPVEELRFATEISETVKRMRRGQNKYDHSPPAPVPTPATSSPVRSRRLTPAARNQSQIYSRRLEFATSQRESSASALLGKRKRRVNPSVAGTVRPTTQNLPGTGAYRGVRKLPSKKGLLEYEMETCALKALDQARQYCNPGFVAWQLDKYLELAGKEYDIEFDQISPEVESEGREERLVNTPQTPPPTDCFTSEFDLCDLIMGSAGSGDDDEDVSRGNPPGSGRRMSNLNLYASYYRKRKSLKSNRTGWPKAQRRRNAGGLGGSRALPDERINFQKMGLAELHPIKQEPMETEEEQTTTTTTTTTTSATRGNLLSKDDEDDEGGGNSPSGGSPADDKQNSREDAVMTPPATDVDEQAEPQADEMESLPDEDETMADSVDQQQDVEAEIEATDADVEEEEEEEDEDEDVFEDAYEEQDMGIQKTEPHEKRARIPSISVTTPPEDSSQGKKLLLTLHNGQRLQATSTPSTGQVQQHQRRTPQLNGSLGSCISPSEKLGDNSDIFTVSSDGLDTDLDLSNTQAGDSHEHCPHQTTPKRKFDISKYAPPNSGKAASSCAAEAATAAVKSLAISQFLKKETCASSSSMRNAWRRTQRRAISAACISAAPSARVPN

Target ORF information:

RefSeq Version	NM_078859.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chiffon (chif), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078859.4

ORF Insert Sequence:

ATGCAACCGC AGTCGGACAA ACAAAGCGCC AGCAGACTCG CAACAACAAC TAGCCACTCA 
ACAGCAGCAG CTTCCGCAAC AGCAGCAACT CCTCCTAAAG TAAAGGTCAT AAAGAGCAAA 
CGCCCCCTGT GCCACTTTAA GTTCTACCTG GACATTTGCG ATCATCAGTT AGCAAAGCGC 
ATTGAGTCCG ACATCAAAGC ATTGGGTGGA CATCTCGAGT TCTTTCTCAG TGACGATATA 
ACACATTTTG TCACTGATAA GCCAGAGGTC ATAGGCGGAA CCTCCGGAAC ACCAGGTACA 
CCTAGCACAC CCGGCACCCC CACGAGTCAC TACCAGCAAA ATGATGGTTC GGCAAGAAAA 
CCGAATCAGA GGCAATCAAG AGCGGATGCA ATACTGAGTC GCGTGCGGCG GAGCACGGTT 
GGAGTCGTAA ACAGTGGCAA TAGCACACCC ACGACATCCC TAAAACGGAG CTATACCATC 
TGGCAGACGG ATTATGCCCA ACGCTTTATA AAGCGCATTC AGACCGAGCT TAAGCAATAC 
CTTGAGGGTA AGAAGGAAGG TGGAGGAGGA TCAACATCAG CTAGCCCCCA TCATATACAG 
CTAAAAAAGC AGTATGTGAA GATCGAATCC GTCAAGCGTA ATTATAGACC TTACTACCAT 
CTTATCAAAC AGCCAGACGA CTGGCCAAAG ATAGATTTAA GCAGCGAAGA CGGCGCCTTT 
CGACTACTAA CAAAGTCGAA GACCAAGGAT AAGGAGCATA GCATGACCCG CAAGCCTTTG 
GGTTCACGCA CATCACAGAA AGACAAACAA GCTGCTGGGG AAGCAAAGCC ACTCCAGCAT 
CCTTCTCTGC AGGAACTTAA AAAACAGTCT GCGATTCCGA ATAGTCCGCG TTCCAATTGT 
CGCGAACCAA TAGATTCGAG TGAAAAACAA GGTGGAGTGT GTGAGATCTG TAAATTGGAA 
TACGATATCC TGAATATCCA TTTGCAGAGC AAAGATCACG AGCTGTTTGC AAAGAATTCG 
GATAATTTTC TGGCTCTAGA CACTCTAATT CAAAGTTCGG CGGATGTGAA TAGGTTCCTG 
GAGGAGGAGC CCGTAGAGAG TGAACTTGAC ATGGATGTCG ATGAATCGTT GAGTAATGAG 
GAGTTGCAGA GTCCCCGTCA GAGGCCATCG CCAGCGTTGC GGGAGAAGTC CAAAAGGATA 
ACCAAGGGAA AGCATTCGTC AGAAAAGTTT CAAGGAGTTG CAGTGGCATC ACCTCAAACG 
CCATTCCCTG GTGCCAAGAA GGTTCAAGGA AATTCACCTG GTAGTCTTTC TGAACTTCAG 
CGTCAGGAAC ATCCAACAAC AGCCGCAGCA ACGCCAACAA CGAATTCAGG ACGAAGGAAA 
ACACAGAACT CCGGTCTGTC ACCCCCTAAG AGAGCCATGT TGCCGCCTTC TTCTATTTAT 
AAAGTAGTAG AAACTAGGGA GGAATGTGCT ACGCCTCCCA GAGGGCGAGG AAGACCTCCC 
AACCAAGTGG ATTCGCCCTC GCTGATTGTA AAGTTCCAAA AGATCCGACA AACAGAGTTA 
CAACGACTCA ATGGAGAGGC AGAGAACTTT ATGTTTCCCA GAACAGCGGT GCCAACTACA 
AGGAGCAGCA GTGAGCTGCC CACGGATGTC GATCGACAAA CCACCTCGGA TGTAAGAGGC 
AGATACAGTA TCTCCTCCGC CAGCCTAGAC ACTAGCACCA GCGAAGCAGA AACTAAGGAA 
TCTTCAGGAT TACCTACTAG CATACGCAAG CGAGCTCAAG CAGTGGGAAG GCGAAGAAAA 
GTAGGAGGAG CAGCGGCGCA GGATGTTTTC CAACGCCAAT TATCAACGGG GAGTAGTAGC 
AGCAATAGTA ACCAGCAGCG CTTTCCAAGC GCCCCCATCC AGCCAGAAGA AGGGCCTCAA 
CCGCAGCCAA AACCGCAGCT AAAGATCAAG ATAAAGCAGG AGCAATTGGT GGCCACCAGG 
AAAAGCAGTC GCACAGCTAC GGCTATTGTG ACAGCAGCCA CCGCCAGCAG TCATCAGCAG 
CAGCAGCTAA GGCAGACAAC TTGCCGGAAA ATGGCCAATA AACTGGAGGA TAGGATGGGG 
GAATTAGTAA AGCCCAAAAT AAAAATAAAG AAGGAAGTGA TCGAAGAGCA GAAAGTGAAG 
GAGTTGGAAG ATCTGGAAGA GATATTAGAT AAAGAATTAG ATGAGGAGGT GGACAGTAGT 
TGCAGTAGTG GTAGCGATGA AGACTACATT GCTGGTAGCC AACGAAGAAT CACGGCAGCT 
CCTCGTAAAT CAACAGATAC TCGTGAACAA AGAGCAGCTA GGCGGCTTTC CAGATTAACC 
ATAAATCGTA GTGCCGGAGA GCTGGAGTTA ACGGAGGTCA AAACGTCTCC TTCGAAAAGC 
CGCACTAAGA TCCAGAAACC GTCAAGTCCC ACGAAAAATA AAGTTAAACA AACGAAGGCA 
GTGCCACCAG CGATAGATTT ATTCTTTGAT TGCAGTAAAA GCGAAAGACT TCGGGAAATG 
CAGTATACCT TCGAATCGTT GCCAAGTGGG GAGCTTTGGA ATAGAGTTTT TCTGCGACAG 
GATGCGGGTG AAGAGAACTA TTACACGTAT TATGGTAGCA CTAATTATAG GAAACTGCCC 
TATGAAATGG GTCCCATACC CATGGCCAAA ACATTGCCAG CACACAGTTG TGCATTATGC 
CGCGAGGCAA GCGAAGTAAA GCAGGATAAG GGAGAGCAGA TCAAGCTGGA AGATCAGAAG 
CCCGCGCCCA AGAAAGAAGT TAAGAAGGAG GAGGAAGTCC AGTCCTCTTC CTCATCCGCG 
ACCTACAAGA ACAAAAAGCT CCATCTGCTC CAACGTTATC AGCAGGAACA GGAGCAACTG 
CAGCAGTTGG AGGGAAATTC CTTGGCCACT GCCGGAGCCA AATGTGATAG CAAGGCGAGC 
ACACCAGAAT TACTGGAAAG GGAGTTTGCC TCGGGTTCTA TGGGGGATAG AGTGCAGCTG 
ATAGAGAGAG TGAGGAGCAC ATCCAGTTCA AGCTGCAGTA ACAGTCAACG GAGCGGCATA 
ACCTGCAGGA ATAAGCAGCT GGCCCGGATT GCCGAATTAC CACCAAGAAA ATCCCCTAGA 
GAACATGCTT CTACTCTGGC TTTGGTCAGT TGCATTATAC GGCAGCGTCA GGACTCACAA 
AGTAAAACGA ACTCAGAAGC AGAAGAGCCA CCTCCTCCTG TAGCCGCACC AAAGCTTAAA 
ACTCCGATAA AACAGGAACC AGTAGCACCT TCATCGCCCC GAACGACTCG TTCTCAAGCT 
GCGACTCCTG TTGAGGAACT ACGATTTGCC ACCGAGATTA GCGAGACAGT AAAGAGAATG 
AGGCGGGGGC AAAACAAATA TGATCATTCC CCGCCAGCGC CAGTTCCTAC CCCCGCAACT 
TCTTCACCCG TTAGAAGTCG CCGCCTGACA CCAGCGGCTC GTAATCAGAG TCAGATTTAT 
TCGCGCCGAT TGGAATTCGC AACCAGTCAA AGGGAATCCT CAGCAAGTGC GCTTCTGGGT 
AAGCGTAAAA GAAGAGTTAA TCCTTCCGTT GCAGGCACAG TGCGACCGAC TACCCAAAAT 
CTTCCCGGAA CGGGTGCGTA TCGTGGAGTC CGCAAATTGC CCAGCAAGAA GGGATTGCTC 
GAATACGAAA TGGAAACGTG TGCCCTTAAA GCTTTGGATC AAGCCCGTCA GTATTGCAAT 
CCTGGCTTCG TTGCCTGGCA GTTAGATAAA TATCTGGAGC TAGCTGGCAA GGAGTACGAT 
ATAGAATTTG ACCAGATATC ACCGGAGGTG GAGTCTGAAG GACGAGAAGA AAGACTTGTT 
AACACACCAC AAACTCCACC ACCTACTGAT TGCTTTACAA GCGAGTTTGA CTTGTGCGAT 
CTAATTATGG GTAGTGCAGG GAGTGGAGAT GATGATGAAG ATGTATCCAG AGGAAACCCA 
CCTGGTTCCG GTCGACGAAT GAGTAATCTC AATCTATACG CCAGCTATTA TAGGAAAAGG 
AAGAGCCTGA AGTCCAATCG CACGGGTTGG CCTAAAGCTC AGAGAAGACG GAACGCAGGC 
GGATTAGGTG GCAGTCGAGC TTTGCCAGAT GAGCGAATTA ATTTCCAGAA AATGGGTCTT 
GCTGAGCTAC ACCCTATAAA GCAGGAGCCA ATGGAAACGG AAGAGGAACA GACCACAACC 
ACTACCACAA CTACTACGAC TTCTGCGACG AGAGGAAATC TCCTGAGCAA AGACGATGAA 
GATGATGAAG GAGGCGGAAA CTCGCCCTCT GGTGGATCAC CTGCTGATGA TAAACAGAAT 
TCCCGCGAAG ATGCCGTGAT GACTCCGCCA GCGACCGATG TTGACGAGCA GGCTGAACCG 
CAGGCGGATG AAATGGAAAG TCTGCCCGAC GAAGATGAAA CAATGGCGGA TTCTGTGGAT 
CAGCAGCAGG ATGTTGAGGC GGAAATCGAA GCAACTGATG CGGATGTCGA GGAAGAAGAA 
GAGGAGGAGG ACGAGGACGA AGATGTTTTT GAAGATGCCT ACGAAGAGCA GGACATGGGC 
ATTCAGAAAA CTGAGCCTCA TGAAAAAAGA GCTCGAATAC CTTCCATATC GGTAACCACG 
CCGCCTGAAG ACTCTTCACA GGGCAAAAAA CTCTTGCTTA CTCTGCATAA TGGCCAGCGA 
CTACAGGCCA CCTCCACACC AAGTACCGGG CAAGTTCAAC AGCATCAGAG AAGAACTCCC 
CAGCTGAACG GAAGTCTAGG CAGCTGTATT TCGCCAAGTG AGAAACTGGG TGACAATTCC 
GACATATTCA CGGTATCATC TGACGGCTTG GATACCGATT TGGATTTAAG CAACACCCAG 
GCAGGCGATT CGCATGAGCA TTGCCCACAC CAGACGACGC CGAAGCGGAA GTTTGACATC 
TCCAAGTATG CGCCACCGAA TAGTGGAAAG GCGGCGAGCA GTTGTGCCGC CGAAGCGGCG 
ACGGCTGCCG TGAAGTCATT GGCCATCTCG CAGTTCCTGA AGAAGGAGAC TTGCGCAAGC 
TCATCAAGCA TGAGGAACGC CTGGCGGAGA ACGCAAAGGA GAGCGATTTC AGCGGCCTGC 
ATAAGCGCGG CACCGTCAGC TCGCGTGCCA AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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chif cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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