nln cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	nln
Entrez Gene ID	449659
Full Name	neurolysin (metallopeptidase M3 family)
Synonyms	zgc:92589
General protein information	Preferred Names neurolysin (metallopeptidase M3 family) Names neurolysin, mitochondrial
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005155569.2	XP_005155626.1	neurolysin, mitochondrial isoform X2
NM_001005930.1	NP_001005930.1	neurolysin, mitochondrial
XM_005155567.4	XP_005155624.1	neurolysin, mitochondrial isoform X1
XM_005155569.3	XP_005155626.1	neurolysin, mitochondrial isoform X2
XM_017357959.1	XP_017213448.1	neurolysin, mitochondrial isoform X3
XM_005155567.3	XP_005155624.1	neurolysin, mitochondrial isoform X1
XM_017357959.2	XP_017213448.1	neurolysin, mitochondrial isoform X3

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

A reference growth curve for nutritional experiments in zebrafish (Danio rerio) and changes in whole body proteome during development.
G?mez-Requeni P, Concei??o LE, Olderbakk Jordal AE, R?nnestad I
Fish physiology and biochemistry36(4)1199-215(2010 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following nln gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nln cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa32606	NM_001005930.1 Latest version!	Danio rerio neurolysin (metallopeptidase M3 family) (nln), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa15895	XM_005155567.4 Latest version!	Danio rerio neurolysin (metallopeptidase M3 family) (nln), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa15897	XM_005155569.3 Latest version!	Danio rerio neurolysin (metallopeptidase M3 family) (nln), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa54019	XM_017357959.2 Latest version!	Danio rerio neurolysin (metallopeptidase M3 family) (nln), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
ODa15897	XM_005155569.2	Danio rerio neurolysin (metallopeptidase M3 family) (nln), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa15895	XM_005155567.3	Danio rerio neurolysin (metallopeptidase M3 family) (nln), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa54019	XM_017357959.1	Danio rerio neurolysin (metallopeptidase M3 family) (nln), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nln ( NM_001005930.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001005930.1 Danio rerio neurolysin (metallopeptidase M3 family) (nln), mRNA.

Price & Availability↑

CloneID	ODa32606
Clone ID Related Accession (Same CDS sequence)	NM_001005930.1
Accession Version	NM_001005930.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1983bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	neurolysin, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC083527.1. ##Evidence-Data-START## Transcript exon combination :: BC083527.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168448, SAMEA4476731 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGTTTATCT TCAGCTTTTC TGTGGCCGGA GCGGTTAGAA GGGTCATTTT ATCCAGCTCA 
AGGTTTAAGA TGACACTTCA AAATGGATCA GTCATATCCG CCCACAGCTG CTCCAGTACA 
GCTGACAAGA ACAACAACAA CCTGCGCTGG GATTTGACCC CAGAAGAGAT CAGACATCGG 
ACAGAGAAAC TGATCCAGAG GATAAAACAA GCCTTTGACA CGGTCGGGTC AGTGGATATT 
GGAAAGGTGT GCTTTGAGAA CACTCTGCAG GTCATTGCAG ACGCCAAAGC AGATTATGCT 
GCTTCTCGTC ATGTGTTGGA CTTCCCCCAG TATGTCGCTC CATGTAAAGA GGTCAGGTTT 
GCGAGCACTG AGGCTGATAA ACGTCTTTCA GATTTCGATG TTGAAGTGAG CATGAGGGCG 
GACGTGTTTC AGAGACTTTT AGTCCTGCAG GAAAAACAGT CCTGTGATTT ACTGCCTGAG 
TCAGAGAGAT TCCTGGGACG ACTTATAAAG TTAGGAAAAC GGAATGGATT ACATTTGTCT 
GAGGATATAC AAGATGAAAT TAAGTCAATT TCTAAGCAAA TAAGTGAACT CTCCATAGAT 
TTCAACCGAA ATCTGAATGA AGAAAATACA GTGCTTTTGT TCTCGAAAGA AGATCTTGTT 
GGTCTTGCCG AAAGCTACGC GAACGGCCTG GAAAAAACGG AAGATGGACA GTACAAAGTT 
ACCCTCGCGT ATCCTCACTA CTTTCCTTTA ATGAAAAGAT GTCATGTCCC TGAAACCAGG 
AGGAAGATGG AGAAGGCCTT TCATAGCAGA TGCAAAGATG TGAACACTGC TATTCTAGAA 
AAGCTTATTG AGCTGCGGGC AAGGCTAGCC AATCTACTGG GCTTCAGCAG TCATGCCAAT 
TACATGCTGG AAATGACCAT GGCCAAAAAC TCAGAGAACG TGGCCAGATT TCTTGATAAG 
TTGTACGAGA GATTGAGGCC AGTGGGCGAG CAGGAGAGGC ATTACCTGCT GTCACTCAAA 
GATGCAGACT GTGAGAGGCG GGGCTTGGAG TTCGACGGAC AGCTCCATGC GTGGGACATG 
CCCTACTACA TGAACCAGGT GGTGACTGAG GGCCTGCTGG ATATATATCA GGATCTGTTG 
GGTCTAAAGT TCCAGCAAGT GGAGAAACCT CATGTGTGGC ACGACAGCCA CGCTGCATGC 
TTTGGGCTCC AGCCAGGGTG TGTTGGGCCT GATGGGGAAC GTAGGATGCC TGTGGCCGCC 
TTGGTGGCCA ACTTCACCAA GCCCACCAAC ACCTGGCCTT CGCTTCTGCA GCACCACGAG 
GTGGAGACCT TCTTCCACGA GTTTGGACAC ATCATGCATG AGCTCTGCTC CAGGAGCCGA 
TATGCAGACT TCAGTGGGAC ACAGGTGGAG ACAGATTTTG TGGAGGTGCC GTCTCAGATG 
CTGGAGAACT GGGTTTGGGA AAAGGAGCCT CTCAGAAGGA TGTCCCGTCA CTATAAAGAT 
GACAGCCCGA TCCCAGATGC TCTGCTGGAC AAACTCATCG CATCTAGAGT GGCCAACACA 
GGTCTGATGA ACCTCAGGCA GATAGTGCTC AGTAAAGCTG ACCAAACGCT CCACACTAAA 
GACCGAGCAG ACACTGCAGC AGAGTTTGCC AAGCTCAGCA AAGACATCCT GGGAATCCCT 
GCCACACCAG GCACAAACAT GACGGCTAGT TTCAGTCATC TGGCCGGAGG TTATGACGGT 
CAGTACTACA GTTACCTCTG GAGTGAAGTC TACTCCATGG ACATCTTTTT CACTCGCTTC 
AAAAAGGAAG GAATCTTGAA TCCAAAGGTG GGCAGAGAAT ACAGACGTGT GGTTCTGGAA 
GCAGGAGGTT CAGTGGATGG CATGGAAATG CTGAAAACGT TCCTGGGACG TGAACCGTGC 
CAGGATGCAT TTTTTCAGTG TAAAGGACTC ACACAATCAG TTACAGAACA AAACTCGCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001005930.1
CDS	92..2074
Translation	MFIFSFSVAGAVRRVILSSSRFKMTLQNGSVISAHSCSSTADKNNNNLRWDLTPEEIRHRTEKLIQRIKQAFDTVGSVDIGKVCFENTLQVIADAKADYAASRHVLDFPQYVAPCKEVRFASTEADKRLSDFDVEVSMRADVFQRLLVLQEKQSCDLLPESERFLGRLIKLGKRNGLHLSEDIQDEIKSISKQISELSIDFNRNLNEENTVLLFSKEDLVGLAESYANGLEKTEDGQYKVTLAYPHYFPLMKRCHVPETRRKMEKAFHSRCKDVNTAILEKLIELRARLANLLGFSSHANYMLEMTMAKNSENVARFLDKLYERLRPVGEQERHYLLSLKDADCERRGLEFDGQLHAWDMPYYMNQVVTEGLLDIYQDLLGLKFQQVEKPHVWHDSHAACFGLQPGCVGPDGERRMPVAALVANFTKPTNTWPSLLQHHEVETFFHEFGHIMHELCSRSRYADFSGTQVETDFVEVPSQMLENWVWEKEPLRRMSRHYKDDSPIPDALLDKLIASRVANTGLMNLRQIVLSKADQTLHTKDRADTAAEFAKLSKDILGIPATPGTNMTASFSHLAGGYDGQYYSYLWSEVYSMDIFFTRFKKEGILNPKVGREYRRVVLEAGGSVDGMEMLKTFLGREPCQDAFFQCKGLTQSVTEQNSH

Target ORF information:

RefSeq Version	NM_001005930.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neurolysin (metallopeptidase M3 family) (nln), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001005930.1

ORF Insert Sequence:

ATGTTTATCT TCAGCTTTTC TGTGGCCGGA GCGGTTAGAA GGGTCATTTT ATCCAGCTCA 
AGGTTTAAGA TGACACTTCA AAATGGATCA GTCATATCCG CCCACAGCTG CTCCAGTACA 
GCTGACAAGA ACAACAACAA CCTGCGCTGG GATTTGACCC CAGAAGAGAT CAGACATCGG 
ACAGAGAAAC TGATCCAGAG GATAAAACAA GCCTTTGACA CGGTCGGGTC AGTGGATATT 
GGAAAGGTGT GCTTTGAGAA CACTCTGCAG GTCATTGCAG ACGCCAAAGC AGATTATGCT 
GCTTCTCGTC ATGTGTTGGA CTTCCCCCAG TATGTCGCTC CATGTAAAGA GGTCAGGTTT 
GCGAGCACTG AGGCTGATAA ACGTCTTTCA GATTTCGATG TTGAAGTGAG CATGAGGGCG 
GACGTGTTTC AGAGACTTTT AGTCCTGCAG GAAAAACAGT CCTGTGATTT ACTGCCTGAG 
TCAGAGAGAT TCCTGGGACG ACTTATAAAG TTAGGAAAAC GGAATGGATT ACATTTGTCT 
GAGGATATAC AAGATGAAAT TAAGTCAATT TCTAAGCAAA TAAGTGAACT CTCCATAGAT 
TTCAACCGAA ATCTGAATGA AGAAAATACA GTGCTTTTGT TCTCGAAAGA AGATCTTGTT 
GGTCTTGCCG AAAGCTACGC GAACGGCCTG GAAAAAACGG AAGATGGACA GTACAAAGTT 
ACCCTCGCGT ATCCTCACTA CTTTCCTTTA ATGAAAAGAT GTCATGTCCC TGAAACCAGG 
AGGAAGATGG AGAAGGCCTT TCATAGCAGA TGCAAAGATG TGAACACTGC TATTCTAGAA 
AAGCTTATTG AGCTGCGGGC AAGGCTAGCC AATCTACTGG GCTTCAGCAG TCATGCCAAT 
TACATGCTGG AAATGACCAT GGCCAAAAAC TCAGAGAACG TGGCCAGATT TCTTGATAAG 
TTGTACGAGA GATTGAGGCC AGTGGGCGAG CAGGAGAGGC ATTACCTGCT GTCACTCAAA 
GATGCAGACT GTGAGAGGCG GGGCTTGGAG TTCGACGGAC AGCTCCATGC GTGGGACATG 
CCCTACTACA TGAACCAGGT GGTGACTGAG GGCCTGCTGG ATATATATCA GGATCTGTTG 
GGTCTAAAGT TCCAGCAAGT GGAGAAACCT CATGTGTGGC ACGACAGCCA CGCTGCATGC 
TTTGGGCTCC AGCCAGGGTG TGTTGGGCCT GATGGGGAAC GTAGGATGCC TGTGGCCGCC 
TTGGTGGCCA ACTTCACCAA GCCCACCAAC ACCTGGCCTT CGCTTCTGCA GCACCACGAG 
GTGGAGACCT TCTTCCACGA GTTTGGACAC ATCATGCATG AGCTCTGCTC CAGGAGCCGA 
TATGCAGACT TCAGTGGGAC ACAGGTGGAG ACAGATTTTG TGGAGGTGCC GTCTCAGATG 
CTGGAGAACT GGGTTTGGGA AAAGGAGCCT CTCAGAAGGA TGTCCCGTCA CTATAAAGAT 
GACAGCCCGA TCCCAGATGC TCTGCTGGAC AAACTCATCG CATCTAGAGT GGCCAACACA 
GGTCTGATGA ACCTCAGGCA GATAGTGCTC AGTAAAGCTG ACCAAACGCT CCACACTAAA 
GACCGAGCAG ACACTGCAGC AGAGTTTGCC AAGCTCAGCA AAGACATCCT GGGAATCCCT 
GCCACACCAG GCACAAACAT GACGGCTAGT TTCAGTCATC TGGCCGGAGG TTATGACGGT 
CAGTACTACA GTTACCTCTG GAGTGAAGTC TACTCCATGG ACATCTTTTT CACTCGCTTC 
AAAAAGGAAG GAATCTTGAA TCCAAAGGTG GGCAGAGAAT ACAGACGTGT GGTTCTGGAA 
GCAGGAGGTT CAGTGGATGG CATGGAAATG CTGAAAACGT TCCTGGGACG TGAACCGTGC 
CAGGATGCAT TTTTTCAGTG TAAAGGACTC ACACAATCAG TTACAGAACA AAACTCGCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nln ( XM_005155567.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155624.1 Danio rerio neurolysin (metallopeptidase M3 family) (nln), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa15895
Clone ID Related Accession (Same CDS sequence)	XM_005155567.4 , XM_005155567.3
Accession Version	XM_005155567.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neurolysin, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005155567.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTATCT TCAGCTTTTC TGTGGCCGGA GCGGTTAGAA GGGTCATTTT ATCCAGCTCA 
AGGCTCAAGA TGACACTTCA AAATGGATCA GTCATATCCG CCCACAGCTG CTCCAGTACA 
GCTGACAAGA ACAACAACAA CCTGCGCTGG GATTTGACCC CAGAAGAGAT CAGACATCGG 
ACAGAGAAAC TGATCCAGAG GATAAAACAA GCCAATGACA CGGTCGGGTC AGTGGATATT 
GGAAAGGTGT GCTTTGAGAA CACTCTGCAG GTCATTGCAG ACGCCAAAGC AGATTATGCT 
GCTTCTCGTC ATGTGTTGGA CTTCCCCCAG TATGTCGCTC CATGTAAAGA GGTCAGGTTT 
GCGAGCACTG AGGCTGATAA ACGTCTTTCA GATTTCGATG TTGAAGTGAG CATGAGGGCG 
GACGTGTTTC AGAGACTTTT AGTCCTGCAG GAAAAACAGT CCTGTGATTT ACTGCCTGAG 
TCAGAGAGAT TCCTGGGACG ACTTATAAAG TTAGGAAAAC GGAATGGATT ACATTTGTCT 
GAGGATATAC AAGATGAAAT TAAGTCAATT TCTAAGCAAA TAAGTGAACT CTCCATAGAT 
TTCAACCGAA ATCTGAATGA AGAAAATACA GTGCTTTTGT TCTCGAAAGA AGATCTTGTT 
GGTCTTGCCG AAAGCTACGC GAACGGCCTG GAAAAAACGG AAGATGGACA GTACAAAGTT 
ACCCTCGCGT ATCCTCACTA CTTTCCTTTA ATGAAAAGAT GTCATGTCCC TGAAACCAGG 
AGGAAGATGG AGAAGGCCTT TCATAGCAGA TGCAAAGATG TGAACACTGC TATTCTAGAA 
AAGCTTATTG AGCTGCGGGC AAGGCTAGCC AATCTACTGG GCTTCAGCAG TCATGCCAAT 
TACATGCTGG AAATGACCAT GGCCAAAAAC TCAGAGAACG TGGCCAGATT TCTTGATGAG 
TTGTACGAGA GATTGAGGCC AGTGGGCGAG CAGGAGAGGC ATTACCTGCT GTCACTCAAA 
GATGCAGACT GTGAGAGGCG GGGCTTGGAG TTTGACGGAC AGCTCCATGC GTGGGACATG 
CCCTACTACA TGAACCAGGT GGAGCAAGTG AAGTTTGCGG TGGACAAAGA CAAACTGATC 
GAGTACTTCC CTCTGCAGGT GGTGACTGAG GGCCTGCTGG ATATATATCA GGATCTGTTG 
GGTCTAAAGT TCCAGCAAGT GGAAAAACCT CATGTGTGGC ACGACAGCGT CAAACTCTAC 
TCTGTACTCG ACTCTGTGAC TGGAAATCAG ATTGGACAGT TTTACTTGGA CCTACATCCT 
AGGGAGGGAA AGTACAGCCA CGCTGCATGC TTTGGGCTCC AGCCAGGGTG TGTTGGGCCT 
GATGGGGAAC GTAGGATGCC TGTGGCCGCC ATGGTGGCCA ACTTCACCAA GCCCACCAGC 
ACCTGGCCTT CGCTTCTGCA GCACCACGAG GTGGAGACCT TCTTCCACGA GTTTGGACAC 
ATCATGCATG AGCTCTGCTC CAGGAGCCGA TATGCAGACT TCAGTGGGAC ACAGGTGGAG 
ACAGATTTTG TGGAGGTGCC GTCTCAGATG CTGGAGAACT GGGTTTGGGA AAAGGAGCCT 
CTCAGAAGGA TGTCCCGTCA CTATAAAGAT GACAGCCCGA TCCCAGATGC TCTGCTGGAC 
AAACTCATCG CATCTAGAGT GGCCAACACA GGTCTGATGA ACCTCAGGCA GATAGTGCTC 
AGTAAAGCTG ACCAAACGCT CCACACTAAA GACCGAGCAG ACACTGCAGC AGAGTTTGCC 
AAGCTCAGCA AAGACATCCT GGGAATCCCT GCCACACCAG GCACAAACAT GACGGCTAGT 
TTCAGTCATC TGGCCGGAGG TTATGACGGT CAGTACTACA GTTACCTCTG GAGTGAAGTC 
TACTCCATGG ACATCTTTTT CACTCGCTTC AAAAAGGAAG GAATCCTGAA TCCAAAGGTG 
GGCAGAGAAT ACAGACGTGT GGTTCTGGAA GCAGGAGGTT CAGTGGATGG CATGGAAATG 
CTGAAAACGT TCCTGGGACG TGAACCGTGC CAGGATGCAT TTTTTCAGTG TAAAGGACTC 
ACACAATCAG TTACAGAACA AAACTTGCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155624.1
CDS	108..2240
Translation	MFIFSFSVAGAVRRVILSSSRLKMTLQNGSVISAHSCSSTADKNNNNLRWDLTPEEIRHRTEKLIQRIKQANDTVGSVDIGKVCFENTLQVIADAKADYAASRHVLDFPQYVAPCKEVRFASTEADKRLSDFDVEVSMRADVFQRLLVLQEKQSCDLLPESERFLGRLIKLGKRNGLHLSEDIQDEIKSISKQISELSIDFNRNLNEENTVLLFSKEDLVGLAESYANGLEKTEDGQYKVTLAYPHYFPLMKRCHVPETRRKMEKAFHSRCKDVNTAILEKLIELRARLANLLGFSSHANYMLEMTMAKNSENVARFLDELYERLRPVGEQERHYLLSLKDADCERRGLEFDGQLHAWDMPYYMNQVEQVKFAVDKDKLIEYFPLQVVTEGLLDIYQDLLGLKFQQVEKPHVWHDSVKLYSVLDSVTGNQIGQFYLDLHPREGKYSHAACFGLQPGCVGPDGERRMPVAAMVANFTKPTSTWPSLLQHHEVETFFHEFGHIMHELCSRSRYADFSGTQVETDFVEVPSQMLENWVWEKEPLRRMSRHYKDDSPIPDALLDKLIASRVANTGLMNLRQIVLSKADQTLHTKDRADTAAEFAKLSKDILGIPATPGTNMTASFSHLAGGYDGQYYSYLWSEVYSMDIFFTRFKKEGILNPKVGREYRRVVLEAGGSVDGMEMLKTFLGREPCQDAFFQCKGLTQSVTEQNLH

Target ORF information:

RefSeq Version	XM_005155567.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neurolysin (metallopeptidase M3 family) (nln), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155567.4

ORF Insert Sequence:

ATGTTTATCT TCAGCTTTTC TGTGGCCGGA GCGGTTAGAA GGGTCATTTT ATCCAGCTCA 
AGGCTCAAGA TGACACTTCA AAATGGATCA GTCATATCCG CCCACAGCTG CTCCAGTACA 
GCTGACAAGA ACAACAACAA CCTGCGCTGG GATTTGACCC CAGAAGAGAT CAGACATCGG 
ACAGAGAAAC TGATCCAGAG GATAAAACAA GCCAATGACA CGGTCGGGTC AGTGGATATT 
GGAAAGGTGT GCTTTGAGAA CACTCTGCAG GTCATTGCAG ACGCCAAAGC AGATTATGCT 
GCTTCTCGTC ATGTGTTGGA CTTCCCCCAG TATGTCGCTC CATGTAAAGA GGTCAGGTTT 
GCGAGCACTG AGGCTGATAA ACGTCTTTCA GATTTCGATG TTGAAGTGAG CATGAGGGCG 
GACGTGTTTC AGAGACTTTT AGTCCTGCAG GAAAAACAGT CCTGTGATTT ACTGCCTGAG 
TCAGAGAGAT TCCTGGGACG ACTTATAAAG TTAGGAAAAC GGAATGGATT ACATTTGTCT 
GAGGATATAC AAGATGAAAT TAAGTCAATT TCTAAGCAAA TAAGTGAACT CTCCATAGAT 
TTCAACCGAA ATCTGAATGA AGAAAATACA GTGCTTTTGT TCTCGAAAGA AGATCTTGTT 
GGTCTTGCCG AAAGCTACGC GAACGGCCTG GAAAAAACGG AAGATGGACA GTACAAAGTT 
ACCCTCGCGT ATCCTCACTA CTTTCCTTTA ATGAAAAGAT GTCATGTCCC TGAAACCAGG 
AGGAAGATGG AGAAGGCCTT TCATAGCAGA TGCAAAGATG TGAACACTGC TATTCTAGAA 
AAGCTTATTG AGCTGCGGGC AAGGCTAGCC AATCTACTGG GCTTCAGCAG TCATGCCAAT 
TACATGCTGG AAATGACCAT GGCCAAAAAC TCAGAGAACG TGGCCAGATT TCTTGATGAG 
TTGTACGAGA GATTGAGGCC AGTGGGCGAG CAGGAGAGGC ATTACCTGCT GTCACTCAAA 
GATGCAGACT GTGAGAGGCG GGGCTTGGAG TTTGACGGAC AGCTCCATGC GTGGGACATG 
CCCTACTACA TGAACCAGGT GGAGCAAGTG AAGTTTGCGG TGGACAAAGA CAAACTGATC 
GAGTACTTCC CTCTGCAGGT GGTGACTGAG GGCCTGCTGG ATATATATCA GGATCTGTTG 
GGTCTAAAGT TCCAGCAAGT GGAAAAACCT CATGTGTGGC ACGACAGCGT CAAACTCTAC 
TCTGTACTCG ACTCTGTGAC TGGAAATCAG ATTGGACAGT TTTACTTGGA CCTACATCCT 
AGGGAGGGAA AGTACAGCCA CGCTGCATGC TTTGGGCTCC AGCCAGGGTG TGTTGGGCCT 
GATGGGGAAC GTAGGATGCC TGTGGCCGCC ATGGTGGCCA ACTTCACCAA GCCCACCAGC 
ACCTGGCCTT CGCTTCTGCA GCACCACGAG GTGGAGACCT TCTTCCACGA GTTTGGACAC 
ATCATGCATG AGCTCTGCTC CAGGAGCCGA TATGCAGACT TCAGTGGGAC ACAGGTGGAG 
ACAGATTTTG TGGAGGTGCC GTCTCAGATG CTGGAGAACT GGGTTTGGGA AAAGGAGCCT 
CTCAGAAGGA TGTCCCGTCA CTATAAAGAT GACAGCCCGA TCCCAGATGC TCTGCTGGAC 
AAACTCATCG CATCTAGAGT GGCCAACACA GGTCTGATGA ACCTCAGGCA GATAGTGCTC 
AGTAAAGCTG ACCAAACGCT CCACACTAAA GACCGAGCAG ACACTGCAGC AGAGTTTGCC 
AAGCTCAGCA AAGACATCCT GGGAATCCCT GCCACACCAG GCACAAACAT GACGGCTAGT 
TTCAGTCATC TGGCCGGAGG TTATGACGGT CAGTACTACA GTTACCTCTG GAGTGAAGTC 
TACTCCATGG ACATCTTTTT CACTCGCTTC AAAAAGGAAG GAATCCTGAA TCCAAAGGTG 
GGCAGAGAAT ACAGACGTGT GGTTCTGGAA GCAGGAGGTT CAGTGGATGG CATGGAAATG 
CTGAAAACGT TCCTGGGACG TGAACCGTGC CAGGATGCAT TTTTTCAGTG TAAAGGACTC 
ACACAATCAG TTACAGAACA AAACTTGCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nln ( XM_005155567.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155624.1 Danio rerio neurolysin (metallopeptidase M3 family) (nln), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa15895
Clone ID Related Accession (Same CDS sequence)	XM_005155567.4 , XM_005155567.3
Accession Version	XM_005155567.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	neurolysin, mitochondrial isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005155567.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTATCT TCAGCTTTTC TGTGGCCGGA GCGGTTAGAA GGGTCATTTT ATCCAGCTCA 
AGGCTCAAGA TGACACTTCA AAATGGATCA GTCATATCCG CCCACAGCTG CTCCAGTACA 
GCTGACAAGA ACAACAACAA CCTGCGCTGG GATTTGACCC CAGAAGAGAT CAGACATCGG 
ACAGAGAAAC TGATCCAGAG GATAAAACAA GCCAATGACA CGGTCGGGTC AGTGGATATT 
GGAAAGGTGT GCTTTGAGAA CACTCTGCAG GTCATTGCAG ACGCCAAAGC AGATTATGCT 
GCTTCTCGTC ATGTGTTGGA CTTCCCCCAG TATGTCGCTC CATGTAAAGA GGTCAGGTTT 
GCGAGCACTG AGGCTGATAA ACGTCTTTCA GATTTCGATG TTGAAGTGAG CATGAGGGCG 
GACGTGTTTC AGAGACTTTT AGTCCTGCAG GAAAAACAGT CCTGTGATTT ACTGCCTGAG 
TCAGAGAGAT TCCTGGGACG ACTTATAAAG TTAGGAAAAC GGAATGGATT ACATTTGTCT 
GAGGATATAC AAGATGAAAT TAAGTCAATT TCTAAGCAAA TAAGTGAACT CTCCATAGAT 
TTCAACCGAA ATCTGAATGA AGAAAATACA GTGCTTTTGT TCTCGAAAGA AGATCTTGTT 
GGTCTTGCCG AAAGCTACGC GAACGGCCTG GAAAAAACGG AAGATGGACA GTACAAAGTT 
ACCCTCGCGT ATCCTCACTA CTTTCCTTTA ATGAAAAGAT GTCATGTCCC TGAAACCAGG 
AGGAAGATGG AGAAGGCCTT TCATAGCAGA TGCAAAGATG TGAACACTGC TATTCTAGAA 
AAGCTTATTG AGCTGCGGGC AAGGCTAGCC AATCTACTGG GCTTCAGCAG TCATGCCAAT 
TACATGCTGG AAATGACCAT GGCCAAAAAC TCAGAGAACG TGGCCAGATT TCTTGATGAG 
TTGTACGAGA GATTGAGGCC AGTGGGCGAG CAGGAGAGGC ATTACCTGCT GTCACTCAAA 
GATGCAGACT GTGAGAGGCG GGGCTTGGAG TTTGACGGAC AGCTCCATGC GTGGGACATG 
CCCTACTACA TGAACCAGGT GGAGCAAGTG AAGTTTGCGG TGGACAAAGA CAAACTGATC 
GAGTACTTCC CTCTGCAGGT GGTGACTGAG GGCCTGCTGG ATATATATCA GGATCTGTTG 
GGTCTAAAGT TCCAGCAAGT GGAAAAACCT CATGTGTGGC ACGACAGCGT CAAACTCTAC 
TCTGTACTCG ACTCTGTGAC TGGAAATCAG ATTGGACAGT TTTACTTGGA CCTACATCCT 
AGGGAGGGAA AGTACAGCCA CGCTGCATGC TTTGGGCTCC AGCCAGGGTG TGTTGGGCCT 
GATGGGGAAC GTAGGATGCC TGTGGCCGCC ATGGTGGCCA ACTTCACCAA GCCCACCAGC 
ACCTGGCCTT CGCTTCTGCA GCACCACGAG GTGGAGACCT TCTTCCACGA GTTTGGACAC 
ATCATGCATG AGCTCTGCTC CAGGAGCCGA TATGCAGACT TCAGTGGGAC ACAGGTGGAG 
ACAGATTTTG TGGAGGTGCC GTCTCAGATG CTGGAGAACT GGGTTTGGGA AAAGGAGCCT 
CTCAGAAGGA TGTCCCGTCA CTATAAAGAT GACAGCCCGA TCCCAGATGC TCTGCTGGAC 
AAACTCATCG CATCTAGAGT GGCCAACACA GGTCTGATGA ACCTCAGGCA GATAGTGCTC 
AGTAAAGCTG ACCAAACGCT CCACACTAAA GACCGAGCAG ACACTGCAGC AGAGTTTGCC 
AAGCTCAGCA AAGACATCCT GGGAATCCCT GCCACACCAG GCACAAACAT GACGGCTAGT 
TTCAGTCATC TGGCCGGAGG TTATGACGGT CAGTACTACA GTTACCTCTG GAGTGAAGTC 
TACTCCATGG ACATCTTTTT CACTCGCTTC AAAAAGGAAG GAATCCTGAA TCCAAAGGTG 
GGCAGAGAAT ACAGACGTGT GGTTCTGGAA GCAGGAGGTT CAGTGGATGG CATGGAAATG 
CTGAAAACGT TCCTGGGACG TGAACCGTGC CAGGATGCAT TTTTTCAGTG TAAAGGACTC 
ACACAATCAG TTACAGAACA AAACTTGCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155624.1
CDS	107..2239
Translation	MFIFSFSVAGAVRRVILSSSRLKMTLQNGSVISAHSCSSTADKNNNNLRWDLTPEEIRHRTEKLIQRIKQANDTVGSVDIGKVCFENTLQVIADAKADYAASRHVLDFPQYVAPCKEVRFASTEADKRLSDFDVEVSMRADVFQRLLVLQEKQSCDLLPESERFLGRLIKLGKRNGLHLSEDIQDEIKSISKQISELSIDFNRNLNEENTVLLFSKEDLVGLAESYANGLEKTEDGQYKVTLAYPHYFPLMKRCHVPETRRKMEKAFHSRCKDVNTAILEKLIELRARLANLLGFSSHANYMLEMTMAKNSENVARFLDELYERLRPVGEQERHYLLSLKDADCERRGLEFDGQLHAWDMPYYMNQVEQVKFAVDKDKLIEYFPLQVVTEGLLDIYQDLLGLKFQQVEKPHVWHDSVKLYSVLDSVTGNQIGQFYLDLHPREGKYSHAACFGLQPGCVGPDGERRMPVAAMVANFTKPTSTWPSLLQHHEVETFFHEFGHIMHELCSRSRYADFSGTQVETDFVEVPSQMLENWVWEKEPLRRMSRHYKDDSPIPDALLDKLIASRVANTGLMNLRQIVLSKADQTLHTKDRADTAAEFAKLSKDILGIPATPGTNMTASFSHLAGGYDGQYYSYLWSEVYSMDIFFTRFKKEGILNPKVGREYRRVVLEAGGSVDGMEMLKTFLGREPCQDAFFQCKGLTQSVTEQNLH

Target ORF information:

RefSeq Version	XM_005155567.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neurolysin (metallopeptidase M3 family) (nln), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155567.3

ORF Insert Sequence:

ATGTTTATCT TCAGCTTTTC TGTGGCCGGA GCGGTTAGAA GGGTCATTTT ATCCAGCTCA 
AGGCTCAAGA TGACACTTCA AAATGGATCA GTCATATCCG CCCACAGCTG CTCCAGTACA 
GCTGACAAGA ACAACAACAA CCTGCGCTGG GATTTGACCC CAGAAGAGAT CAGACATCGG 
ACAGAGAAAC TGATCCAGAG GATAAAACAA GCCAATGACA CGGTCGGGTC AGTGGATATT 
GGAAAGGTGT GCTTTGAGAA CACTCTGCAG GTCATTGCAG ACGCCAAAGC AGATTATGCT 
GCTTCTCGTC ATGTGTTGGA CTTCCCCCAG TATGTCGCTC CATGTAAAGA GGTCAGGTTT 
GCGAGCACTG AGGCTGATAA ACGTCTTTCA GATTTCGATG TTGAAGTGAG CATGAGGGCG 
GACGTGTTTC AGAGACTTTT AGTCCTGCAG GAAAAACAGT CCTGTGATTT ACTGCCTGAG 
TCAGAGAGAT TCCTGGGACG ACTTATAAAG TTAGGAAAAC GGAATGGATT ACATTTGTCT 
GAGGATATAC AAGATGAAAT TAAGTCAATT TCTAAGCAAA TAAGTGAACT CTCCATAGAT 
TTCAACCGAA ATCTGAATGA AGAAAATACA GTGCTTTTGT TCTCGAAAGA AGATCTTGTT 
GGTCTTGCCG AAAGCTACGC GAACGGCCTG GAAAAAACGG AAGATGGACA GTACAAAGTT 
ACCCTCGCGT ATCCTCACTA CTTTCCTTTA ATGAAAAGAT GTCATGTCCC TGAAACCAGG 
AGGAAGATGG AGAAGGCCTT TCATAGCAGA TGCAAAGATG TGAACACTGC TATTCTAGAA 
AAGCTTATTG AGCTGCGGGC AAGGCTAGCC AATCTACTGG GCTTCAGCAG TCATGCCAAT 
TACATGCTGG AAATGACCAT GGCCAAAAAC TCAGAGAACG TGGCCAGATT TCTTGATGAG 
TTGTACGAGA GATTGAGGCC AGTGGGCGAG CAGGAGAGGC ATTACCTGCT GTCACTCAAA 
GATGCAGACT GTGAGAGGCG GGGCTTGGAG TTTGACGGAC AGCTCCATGC GTGGGACATG 
CCCTACTACA TGAACCAGGT GGAGCAAGTG AAGTTTGCGG TGGACAAAGA CAAACTGATC 
GAGTACTTCC CTCTGCAGGT GGTGACTGAG GGCCTGCTGG ATATATATCA GGATCTGTTG 
GGTCTAAAGT TCCAGCAAGT GGAAAAACCT CATGTGTGGC ACGACAGCGT CAAACTCTAC 
TCTGTACTCG ACTCTGTGAC TGGAAATCAG ATTGGACAGT TTTACTTGGA CCTACATCCT 
AGGGAGGGAA AGTACAGCCA CGCTGCATGC TTTGGGCTCC AGCCAGGGTG TGTTGGGCCT 
GATGGGGAAC GTAGGATGCC TGTGGCCGCC ATGGTGGCCA ACTTCACCAA GCCCACCAGC 
ACCTGGCCTT CGCTTCTGCA GCACCACGAG GTGGAGACCT TCTTCCACGA GTTTGGACAC 
ATCATGCATG AGCTCTGCTC CAGGAGCCGA TATGCAGACT TCAGTGGGAC ACAGGTGGAG 
ACAGATTTTG TGGAGGTGCC GTCTCAGATG CTGGAGAACT GGGTTTGGGA AAAGGAGCCT 
CTCAGAAGGA TGTCCCGTCA CTATAAAGAT GACAGCCCGA TCCCAGATGC TCTGCTGGAC 
AAACTCATCG CATCTAGAGT GGCCAACACA GGTCTGATGA ACCTCAGGCA GATAGTGCTC 
AGTAAAGCTG ACCAAACGCT CCACACTAAA GACCGAGCAG ACACTGCAGC AGAGTTTGCC 
AAGCTCAGCA AAGACATCCT GGGAATCCCT GCCACACCAG GCACAAACAT GACGGCTAGT 
TTCAGTCATC TGGCCGGAGG TTATGACGGT CAGTACTACA GTTACCTCTG GAGTGAAGTC 
TACTCCATGG ACATCTTTTT CACTCGCTTC AAAAAGGAAG GAATCCTGAA TCCAAAGGTG 
GGCAGAGAAT ACAGACGTGT GGTTCTGGAA GCAGGAGGTT CAGTGGATGG CATGGAAATG 
CTGAAAACGT TCCTGGGACG TGAACCGTGC CAGGATGCAT TTTTTCAGTG TAAAGGACTC 
ACACAATCAG TTACAGAACA AAACTTGCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nln ( XM_005155569.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155626.1 Danio rerio neurolysin (metallopeptidase M3 family) (nln), transcript variant X2, mRNA.

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CloneID	ODa15897
Clone ID Related Accession (Same CDS sequence)	XM_005155569.2 , XM_005155569.3
Accession Version	XM_005155569.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	neurolysin, mitochondrial isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2014 this sequence version replaced XM_005155569.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTTC AAAATGGATC AGTCATATCC GCCCACAGCT GCTCCAGTAC AGCTGACAAG 
AACAACAACA ACCTGCGCTG GGATTTGACC CCAGAAGAGA TCAGACATCG GACAGAGAAA 
CTGATCCAGA GGATAAAACA AGCCAATGAC ACGGTCGGGT CAGTGGATAT TGGAAAGGTG 
TGCTTTGAGA ACACTCTGCA GGTCATTGCA GACGCCAAAG CAGATTATGC TGCTTCTCGT 
CATGTGTTGG ACTTCCCCCA GTATGTCGCT CCATGTAAAG AGGTCAGGTT TGCGAGCACT 
GAGGCTGATA AACGTCTTTC AGATTTCGAT GTTGAAGTGA GCATGAGGGC GGACGTGTTT 
CAGAGACTTT TAGTCCTGCA GGAAAAACAG TCCTGTGATT TACTGCCTGA GTCAGAGAGA 
TTCCTGGGAC GACTTATAAA GTTAGGAAAA CGGAATGGAT TACATTTGTC TGAGGATATA 
CAAGATGAAA TTAAGTCAAT TTCTAAGCAA ATAAGTGAAC TCTCCATAGA TTTCAACCGA 
AATCTGAATG AAGAAAATAC AGTGCTTTTG TTCTCGAAAG AAGATCTTGT TGGTCTTGCC 
GAAAGCTACG CGAACGGCCT GGAAAAAACG GAAGATGGAC AGTACAAAGT TACCCTCGCG 
TATCCTCACT ACTTTCCTTT AATGAAAAGA TGTCATGTCC CTGAAACCAG GAGGAAGATG 
GAGAAGGCCT TTCATAGCAG ATGCAAAGAT GTGAACACTG CTATTCTAGA AAAGCTTATT 
GAGCTGCGGG CAAGGCTAGC CAATCTACTG GGCTTCAGCA GTCATGCCAA TTACATGCTG 
GAAATGACCA TGGCCAAAAA CTCAGAGAAC GTGGCCAGAT TTCTTGATGA GTTGTACGAG 
AGATTGAGGC CAGTGGGCGA GCAGGAGAGG CATTACCTGC TGTCACTCAA AGATGCAGAC 
TGTGAGAGGC GGGGCTTGGA GTTTGACGGA CAGCTCCATG CGTGGGACAT GCCCTACTAC 
ATGAACCAGG TGGAGCAAGT GAAGTTTGCG GTGGACAAAG ACAAACTGAT CGAGTACTTC 
CCTCTGCAGG TGGTGACTGA GGGCCTGCTG GATATATATC AGGATCTGTT GGGTCTAAAG 
TTCCAGCAAG TGGAAAAACC TCATGTGTGG CACGACAGCG TCAAACTCTA CTCTGTACTC 
GACTCTGTGA CTGGAAATCA GATTGGACAG TTTTACTTGG ACCTACATCC TAGGGAGGGA 
AAGTACAGCC ACGCTGCATG CTTTGGGCTC CAGCCAGGGT GTGTTGGGCC TGATGGGGAA 
CGTAGGATGC CTGTGGCCGC CATGGTGGCC AACTTCACCA AGCCCACCAG CACCTGGCCT 
TCGCTTCTGC AGCACCACGA GGTGGAGACC TTCTTCCACG AGTTTGGACA CATCATGCAT 
GAGCTCTGCT CCAGGAGCCG ATATGCAGAC TTCAGTGGGA CACAGGTGGA GACAGATTTT 
GTGGAGGTGC CGTCTCAGAT GCTGGAGAAC TGGGTTTGGG AAAAGGAGCC TCTCAGAAGG 
ATGTCCCGTC ACTATAAAGA TGACAGCCCG ATCCCAGATG CTCTGCTGGA CAAACTCATC 
GCATCTAGAG TGGCCAACAC AGGTCTGATG AACCTCAGGC AGATAGTGCT CAGTAAAGCT 
GACCAAACGC TCCACACTAA AGACCGAGCA GACACTGCAG CAGAGTTTGC CAAGCTCAGC 
AAAGACATCC TGGGAATCCC TGCCACACCA GGCACAAACA TGACGGCTAG TTTCAGTCAT 
CTGGCCGGAG GTTATGACGG TCAGTACTAC AGTTACCTCT GGAGTGAAGT CTACTCCATG 
GACATCTTTT TCACTCGCTT CAAAAAGGAA GGAATCCTGA ATCCAAAGGT GGGCAGAGAA 
TACAGACGTG TGGTTCTGGA AGCAGGAGGT TCAGTGGATG GCATGGAAAT GCTGAAAACG 
TTCCTGGGAC GTGAACCGTG CCAGGATGCA TTTTTTCAGT GTAAAGGACT CACACAATCA 
GTTACAGAAC AAAACTTGCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155626.1
CDS	91..2154
Translation	MTLQNGSVISAHSCSSTADKNNNNLRWDLTPEEIRHRTEKLIQRIKQANDTVGSVDIGKVCFENTLQVIADAKADYAASRHVLDFPQYVAPCKEVRFASTEADKRLSDFDVEVSMRADVFQRLLVLQEKQSCDLLPESERFLGRLIKLGKRNGLHLSEDIQDEIKSISKQISELSIDFNRNLNEENTVLLFSKEDLVGLAESYANGLEKTEDGQYKVTLAYPHYFPLMKRCHVPETRRKMEKAFHSRCKDVNTAILEKLIELRARLANLLGFSSHANYMLEMTMAKNSENVARFLDELYERLRPVGEQERHYLLSLKDADCERRGLEFDGQLHAWDMPYYMNQVEQVKFAVDKDKLIEYFPLQVVTEGLLDIYQDLLGLKFQQVEKPHVWHDSVKLYSVLDSVTGNQIGQFYLDLHPREGKYSHAACFGLQPGCVGPDGERRMPVAAMVANFTKPTSTWPSLLQHHEVETFFHEFGHIMHELCSRSRYADFSGTQVETDFVEVPSQMLENWVWEKEPLRRMSRHYKDDSPIPDALLDKLIASRVANTGLMNLRQIVLSKADQTLHTKDRADTAAEFAKLSKDILGIPATPGTNMTASFSHLAGGYDGQYYSYLWSEVYSMDIFFTRFKKEGILNPKVGREYRRVVLEAGGSVDGMEMLKTFLGREPCQDAFFQCKGLTQSVTEQNLH

Target ORF information:

RefSeq Version	XM_005155569.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neurolysin (metallopeptidase M3 family) (nln), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155569.2

ORF Insert Sequence:

ATGACACTTC AAAATGGATC AGTCATATCC GCCCACAGCT GCTCCAGTAC AGCTGACAAG 
AACAACAACA ACCTGCGCTG GGATTTGACC CCAGAAGAGA TCAGACATCG GACAGAGAAA 
CTGATCCAGA GGATAAAACA AGCCAATGAC ACGGTCGGGT CAGTGGATAT TGGAAAGGTG 
TGCTTTGAGA ACACTCTGCA GGTCATTGCA GACGCCAAAG CAGATTATGC TGCTTCTCGT 
CATGTGTTGG ACTTCCCCCA GTATGTCGCT CCATGTAAAG AGGTCAGGTT TGCGAGCACT 
GAGGCTGATA AACGTCTTTC AGATTTCGAT GTTGAAGTGA GCATGAGGGC GGACGTGTTT 
CAGAGACTTT TAGTCCTGCA GGAAAAACAG TCCTGTGATT TACTGCCTGA GTCAGAGAGA 
TTCCTGGGAC GACTTATAAA GTTAGGAAAA CGGAATGGAT TACATTTGTC TGAGGATATA 
CAAGATGAAA TTAAGTCAAT TTCTAAGCAA ATAAGTGAAC TCTCCATAGA TTTCAACCGA 
AATCTGAATG AAGAAAATAC AGTGCTTTTG TTCTCGAAAG AAGATCTTGT TGGTCTTGCC 
GAAAGCTACG CGAACGGCCT GGAAAAAACG GAAGATGGAC AGTACAAAGT TACCCTCGCG 
TATCCTCACT ACTTTCCTTT AATGAAAAGA TGTCATGTCC CTGAAACCAG GAGGAAGATG 
GAGAAGGCCT TTCATAGCAG ATGCAAAGAT GTGAACACTG CTATTCTAGA AAAGCTTATT 
GAGCTGCGGG CAAGGCTAGC CAATCTACTG GGCTTCAGCA GTCATGCCAA TTACATGCTG 
GAAATGACCA TGGCCAAAAA CTCAGAGAAC GTGGCCAGAT TTCTTGATGA GTTGTACGAG 
AGATTGAGGC CAGTGGGCGA GCAGGAGAGG CATTACCTGC TGTCACTCAA AGATGCAGAC 
TGTGAGAGGC GGGGCTTGGA GTTTGACGGA CAGCTCCATG CGTGGGACAT GCCCTACTAC 
ATGAACCAGG TGGAGCAAGT GAAGTTTGCG GTGGACAAAG ACAAACTGAT CGAGTACTTC 
CCTCTGCAGG TGGTGACTGA GGGCCTGCTG GATATATATC AGGATCTGTT GGGTCTAAAG 
TTCCAGCAAG TGGAAAAACC TCATGTGTGG CACGACAGCG TCAAACTCTA CTCTGTACTC 
GACTCTGTGA CTGGAAATCA GATTGGACAG TTTTACTTGG ACCTACATCC TAGGGAGGGA 
AAGTACAGCC ACGCTGCATG CTTTGGGCTC CAGCCAGGGT GTGTTGGGCC TGATGGGGAA 
CGTAGGATGC CTGTGGCCGC CATGGTGGCC AACTTCACCA AGCCCACCAG CACCTGGCCT 
TCGCTTCTGC AGCACCACGA GGTGGAGACC TTCTTCCACG AGTTTGGACA CATCATGCAT 
GAGCTCTGCT CCAGGAGCCG ATATGCAGAC TTCAGTGGGA CACAGGTGGA GACAGATTTT 
GTGGAGGTGC CGTCTCAGAT GCTGGAGAAC TGGGTTTGGG AAAAGGAGCC TCTCAGAAGG 
ATGTCCCGTC ACTATAAAGA TGACAGCCCG ATCCCAGATG CTCTGCTGGA CAAACTCATC 
GCATCTAGAG TGGCCAACAC AGGTCTGATG AACCTCAGGC AGATAGTGCT CAGTAAAGCT 
GACCAAACGC TCCACACTAA AGACCGAGCA GACACTGCAG CAGAGTTTGC CAAGCTCAGC 
AAAGACATCC TGGGAATCCC TGCCACACCA GGCACAAACA TGACGGCTAG TTTCAGTCAT 
CTGGCCGGAG GTTATGACGG TCAGTACTAC AGTTACCTCT GGAGTGAAGT CTACTCCATG 
GACATCTTTT TCACTCGCTT CAAAAAGGAA GGAATCCTGA ATCCAAAGGT GGGCAGAGAA 
TACAGACGTG TGGTTCTGGA AGCAGGAGGT TCAGTGGATG GCATGGAAAT GCTGAAAACG 
TTCCTGGGAC GTGAACCGTG CCAGGATGCA TTTTTTCAGT GTAAAGGACT CACACAATCA 
GTTACAGAAC AAAACTTGCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nln ( XM_005155569.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155626.1 Danio rerio neurolysin (metallopeptidase M3 family) (nln), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa15897
Clone ID Related Accession (Same CDS sequence)	XM_005155569.2 , XM_005155569.3
Accession Version	XM_005155569.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neurolysin, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005155569.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTTC AAAATGGATC AGTCATATCC GCCCACAGCT GCTCCAGTAC AGCTGACAAG 
AACAACAACA ACCTGCGCTG GGATTTGACC CCAGAAGAGA TCAGACATCG GACAGAGAAA 
CTGATCCAGA GGATAAAACA AGCCAATGAC ACGGTCGGGT CAGTGGATAT TGGAAAGGTG 
TGCTTTGAGA ACACTCTGCA GGTCATTGCA GACGCCAAAG CAGATTATGC TGCTTCTCGT 
CATGTGTTGG ACTTCCCCCA GTATGTCGCT CCATGTAAAG AGGTCAGGTT TGCGAGCACT 
GAGGCTGATA AACGTCTTTC AGATTTCGAT GTTGAAGTGA GCATGAGGGC GGACGTGTTT 
CAGAGACTTT TAGTCCTGCA GGAAAAACAG TCCTGTGATT TACTGCCTGA GTCAGAGAGA 
TTCCTGGGAC GACTTATAAA GTTAGGAAAA CGGAATGGAT TACATTTGTC TGAGGATATA 
CAAGATGAAA TTAAGTCAAT TTCTAAGCAA ATAAGTGAAC TCTCCATAGA TTTCAACCGA 
AATCTGAATG AAGAAAATAC AGTGCTTTTG TTCTCGAAAG AAGATCTTGT TGGTCTTGCC 
GAAAGCTACG CGAACGGCCT GGAAAAAACG GAAGATGGAC AGTACAAAGT TACCCTCGCG 
TATCCTCACT ACTTTCCTTT AATGAAAAGA TGTCATGTCC CTGAAACCAG GAGGAAGATG 
GAGAAGGCCT TTCATAGCAG ATGCAAAGAT GTGAACACTG CTATTCTAGA AAAGCTTATT 
GAGCTGCGGG CAAGGCTAGC CAATCTACTG GGCTTCAGCA GTCATGCCAA TTACATGCTG 
GAAATGACCA TGGCCAAAAA CTCAGAGAAC GTGGCCAGAT TTCTTGATGA GTTGTACGAG 
AGATTGAGGC CAGTGGGCGA GCAGGAGAGG CATTACCTGC TGTCACTCAA AGATGCAGAC 
TGTGAGAGGC GGGGCTTGGA GTTTGACGGA CAGCTCCATG CGTGGGACAT GCCCTACTAC 
ATGAACCAGG TGGAGCAAGT GAAGTTTGCG GTGGACAAAG ACAAACTGAT CGAGTACTTC 
CCTCTGCAGG TGGTGACTGA GGGCCTGCTG GATATATATC AGGATCTGTT GGGTCTAAAG 
TTCCAGCAAG TGGAAAAACC TCATGTGTGG CACGACAGCG TCAAACTCTA CTCTGTACTC 
GACTCTGTGA CTGGAAATCA GATTGGACAG TTTTACTTGG ACCTACATCC TAGGGAGGGA 
AAGTACAGCC ACGCTGCATG CTTTGGGCTC CAGCCAGGGT GTGTTGGGCC TGATGGGGAA 
CGTAGGATGC CTGTGGCCGC CATGGTGGCC AACTTCACCA AGCCCACCAG CACCTGGCCT 
TCGCTTCTGC AGCACCACGA GGTGGAGACC TTCTTCCACG AGTTTGGACA CATCATGCAT 
GAGCTCTGCT CCAGGAGCCG ATATGCAGAC TTCAGTGGGA CACAGGTGGA GACAGATTTT 
GTGGAGGTGC CGTCTCAGAT GCTGGAGAAC TGGGTTTGGG AAAAGGAGCC TCTCAGAAGG 
ATGTCCCGTC ACTATAAAGA TGACAGCCCG ATCCCAGATG CTCTGCTGGA CAAACTCATC 
GCATCTAGAG TGGCCAACAC AGGTCTGATG AACCTCAGGC AGATAGTGCT CAGTAAAGCT 
GACCAAACGC TCCACACTAA AGACCGAGCA GACACTGCAG CAGAGTTTGC CAAGCTCAGC 
AAAGACATCC TGGGAATCCC TGCCACACCA GGCACAAACA TGACGGCTAG TTTCAGTCAT 
CTGGCCGGAG GTTATGACGG TCAGTACTAC AGTTACCTCT GGAGTGAAGT CTACTCCATG 
GACATCTTTT TCACTCGCTT CAAAAAGGAA GGAATCCTGA ATCCAAAGGT GGGCAGAGAA 
TACAGACGTG TGGTTCTGGA AGCAGGAGGT TCAGTGGATG GCATGGAAAT GCTGAAAACG 
TTCCTGGGAC GTGAACCGTG CCAGGATGCA TTTTTTCAGT GTAAAGGACT CACACAATCA 
GTTACAGAAC AAAACTTGCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155626.1
CDS	91..2154
Translation	MTLQNGSVISAHSCSSTADKNNNNLRWDLTPEEIRHRTEKLIQRIKQANDTVGSVDIGKVCFENTLQVIADAKADYAASRHVLDFPQYVAPCKEVRFASTEADKRLSDFDVEVSMRADVFQRLLVLQEKQSCDLLPESERFLGRLIKLGKRNGLHLSEDIQDEIKSISKQISELSIDFNRNLNEENTVLLFSKEDLVGLAESYANGLEKTEDGQYKVTLAYPHYFPLMKRCHVPETRRKMEKAFHSRCKDVNTAILEKLIELRARLANLLGFSSHANYMLEMTMAKNSENVARFLDELYERLRPVGEQERHYLLSLKDADCERRGLEFDGQLHAWDMPYYMNQVEQVKFAVDKDKLIEYFPLQVVTEGLLDIYQDLLGLKFQQVEKPHVWHDSVKLYSVLDSVTGNQIGQFYLDLHPREGKYSHAACFGLQPGCVGPDGERRMPVAAMVANFTKPTSTWPSLLQHHEVETFFHEFGHIMHELCSRSRYADFSGTQVETDFVEVPSQMLENWVWEKEPLRRMSRHYKDDSPIPDALLDKLIASRVANTGLMNLRQIVLSKADQTLHTKDRADTAAEFAKLSKDILGIPATPGTNMTASFSHLAGGYDGQYYSYLWSEVYSMDIFFTRFKKEGILNPKVGREYRRVVLEAGGSVDGMEMLKTFLGREPCQDAFFQCKGLTQSVTEQNLH

Target ORF information:

RefSeq Version	XM_005155569.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neurolysin (metallopeptidase M3 family) (nln), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155569.3

ORF Insert Sequence:

ATGACACTTC AAAATGGATC AGTCATATCC GCCCACAGCT GCTCCAGTAC AGCTGACAAG 
AACAACAACA ACCTGCGCTG GGATTTGACC CCAGAAGAGA TCAGACATCG GACAGAGAAA 
CTGATCCAGA GGATAAAACA AGCCAATGAC ACGGTCGGGT CAGTGGATAT TGGAAAGGTG 
TGCTTTGAGA ACACTCTGCA GGTCATTGCA GACGCCAAAG CAGATTATGC TGCTTCTCGT 
CATGTGTTGG ACTTCCCCCA GTATGTCGCT CCATGTAAAG AGGTCAGGTT TGCGAGCACT 
GAGGCTGATA AACGTCTTTC AGATTTCGAT GTTGAAGTGA GCATGAGGGC GGACGTGTTT 
CAGAGACTTT TAGTCCTGCA GGAAAAACAG TCCTGTGATT TACTGCCTGA GTCAGAGAGA 
TTCCTGGGAC GACTTATAAA GTTAGGAAAA CGGAATGGAT TACATTTGTC TGAGGATATA 
CAAGATGAAA TTAAGTCAAT TTCTAAGCAA ATAAGTGAAC TCTCCATAGA TTTCAACCGA 
AATCTGAATG AAGAAAATAC AGTGCTTTTG TTCTCGAAAG AAGATCTTGT TGGTCTTGCC 
GAAAGCTACG CGAACGGCCT GGAAAAAACG GAAGATGGAC AGTACAAAGT TACCCTCGCG 
TATCCTCACT ACTTTCCTTT AATGAAAAGA TGTCATGTCC CTGAAACCAG GAGGAAGATG 
GAGAAGGCCT TTCATAGCAG ATGCAAAGAT GTGAACACTG CTATTCTAGA AAAGCTTATT 
GAGCTGCGGG CAAGGCTAGC CAATCTACTG GGCTTCAGCA GTCATGCCAA TTACATGCTG 
GAAATGACCA TGGCCAAAAA CTCAGAGAAC GTGGCCAGAT TTCTTGATGA GTTGTACGAG 
AGATTGAGGC CAGTGGGCGA GCAGGAGAGG CATTACCTGC TGTCACTCAA AGATGCAGAC 
TGTGAGAGGC GGGGCTTGGA GTTTGACGGA CAGCTCCATG CGTGGGACAT GCCCTACTAC 
ATGAACCAGG TGGAGCAAGT GAAGTTTGCG GTGGACAAAG ACAAACTGAT CGAGTACTTC 
CCTCTGCAGG TGGTGACTGA GGGCCTGCTG GATATATATC AGGATCTGTT GGGTCTAAAG 
TTCCAGCAAG TGGAAAAACC TCATGTGTGG CACGACAGCG TCAAACTCTA CTCTGTACTC 
GACTCTGTGA CTGGAAATCA GATTGGACAG TTTTACTTGG ACCTACATCC TAGGGAGGGA 
AAGTACAGCC ACGCTGCATG CTTTGGGCTC CAGCCAGGGT GTGTTGGGCC TGATGGGGAA 
CGTAGGATGC CTGTGGCCGC CATGGTGGCC AACTTCACCA AGCCCACCAG CACCTGGCCT 
TCGCTTCTGC AGCACCACGA GGTGGAGACC TTCTTCCACG AGTTTGGACA CATCATGCAT 
GAGCTCTGCT CCAGGAGCCG ATATGCAGAC TTCAGTGGGA CACAGGTGGA GACAGATTTT 
GTGGAGGTGC CGTCTCAGAT GCTGGAGAAC TGGGTTTGGG AAAAGGAGCC TCTCAGAAGG 
ATGTCCCGTC ACTATAAAGA TGACAGCCCG ATCCCAGATG CTCTGCTGGA CAAACTCATC 
GCATCTAGAG TGGCCAACAC AGGTCTGATG AACCTCAGGC AGATAGTGCT CAGTAAAGCT 
GACCAAACGC TCCACACTAA AGACCGAGCA GACACTGCAG CAGAGTTTGC CAAGCTCAGC 
AAAGACATCC TGGGAATCCC TGCCACACCA GGCACAAACA TGACGGCTAG TTTCAGTCAT 
CTGGCCGGAG GTTATGACGG TCAGTACTAC AGTTACCTCT GGAGTGAAGT CTACTCCATG 
GACATCTTTT TCACTCGCTT CAAAAAGGAA GGAATCCTGA ATCCAAAGGT GGGCAGAGAA 
TACAGACGTG TGGTTCTGGA AGCAGGAGGT TCAGTGGATG GCATGGAAAT GCTGAAAACG 
TTCCTGGGAC GTGAACCGTG CCAGGATGCA TTTTTTCAGT GTAAAGGACT CACACAATCA 
GTTACAGAAC AAAACTTGCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nln ( XM_017357959.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213448.1 Danio rerio neurolysin (metallopeptidase M3 family) (nln), transcript variant X3, mRNA.

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CloneID	ODa54019
Clone ID Related Accession (Same CDS sequence)	XM_017357959.1 , XM_017357959.2
Accession Version	XM_017357959.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	neurolysin, mitochondrial isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACGGT CGGGTCAGTG GATATTGGAA AGGTGTGCTT TGAGAACACT CTGCAGGTCA 
TTGCAGACGC CAAAGCAGAT TATGCTGGAA AAACAGTCCT GTGATTTACT GCCTGAGTCA 
GAGAGATTCC TGGGACGACT TATAAAGTTA GGAAAACGGA ATGGATTACA TTTGTCTGAG 
GATATACAAG ATGAAATTAA GTCAATTTCT AAGCAAATAA GTGAACTCTC CATAGATTTC 
AACCGAAATC TGAATGAAGA AAATACAGTG CTTTTGTTCT CGAAAGAAGA TCTTGTTGGT 
CTTGCCGAAA GCTACGCGAA CGGCCTGGAA AAAACGGAAG ATGGACAGTA CAAAGTTACC 
CTCGCGTATC CTCACTACTT TCCTTTAATG AAAAGATGTC ATGTCCCTGA AACCAGGAGG 
AAGATGGAGA AGGCCTTTCA TAGCAGATGC AAAGATGTGA ACACTGCTAT TCTAGAAAAG 
CTTATTGAGC TGCGGGCAAG GCTAGCCAAT CTACTGGGCT TCAGCAGTCA TGCCAATTAC 
ATGCTGGAAA TGACCATGGC CAAAAACTCA GAGAACGTGG CCAGATTTCT TGATGAGTTG 
TACGAGAGAT TGAGGCCAGT GGGCGAGCAG GAGAGGCATT ACCTGCTGTC ACTCAAAGAT 
GCAGACTGTG AGAGGCGGGG CTTGGAGTTT GACGGACAGC TCCATGCGTG GGACATGCCC 
TACTACATGA ACCAGGTGGA GCAAGTGAAG TTTGCGGTGG ACAAAGACAA ACTGATCGAG 
TACTTCCCTC TGCAGGTGGT GACTGAGGGC CTGCTGGATA TATATCAGGA TCTGTTGGGT 
CTAAAGTTCC AGCAAGTGGA AAAACCTCAT GTGTGGCACG ACAGCGTCAA ACTCTACTCT 
GTACTCGACT CTGTGACTGG AAATCAGATT GGACAGTTTT ACTTGGACCT ACATCCTAGG 
GAGGGAAAGT ACAGCCACGC TGCATGCTTT GGGCTCCAGC CAGGGTGTGT TGGGCCTGAT 
GGGGAACGTA GGATGCCTGT GGCCGCCATG GTGGCCAACT TCACCAAGCC CACCAGCACC 
TGGCCTTCGC TTCTGCAGCA CCACGAGGTG GAGACCTTCT TCCACGAGTT TGGACACATC 
ATGCATGAGC TCTGCTCCAG GAGCCGATAT GCAGACTTCA GTGGGACACA GGTGGAGACA 
GATTTTGTGG AGGTGCCGTC TCAGATGCTG GAGAACTGGG TTTGGGAAAA GGAGCCTCTC 
AGAAGGATGT CCCGTCACTA TAAAGATGAC AGCCCGATCC CAGATGCTCT GCTGGACAAA 
CTCATCGCAT CTAGAGTGGC CAACACAGGT CTGATGAACC TCAGGCAGAT AGTGCTCAGT 
AAAGCTGACC AAACGCTCCA CACTAAAGAC CGAGCAGACA CTGCAGCAGA GTTTGCCAAG 
CTCAGCAAAG ACATCCTGGG AATCCCTGCC ACACCAGGCA CAAACATGAC GGCTAGTTTC 
AGTCATCTGG CCGGAGGTTA TGACGGTCAG TACTACAGTT ACCTCTGGAG TGAAGTCTAC 
TCCATGGACA TCTTTTTCAC TCGCTTCAAA AAGGAAGGAA TCCTGAATCC AAAGGTGGGC 
AGAGAATACA GACGTGTGGT TCTGGAAGCA GGAGGTTCAG TGGATGGCAT GGAAATGCTG 
AAAACGTTCC TGGGACGTGA ACCGTGCCAG GATGCATTTT TTCAGTGTAA AGGACTCACA 
CAATCAGTTA CAGAACAAAA CTTGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213448.1
CDS	174..1943
Translation	MTRSGQWILERCALRTLCRSLQTPKQIMLEKQSCDLLPESERFLGRLIKLGKRNGLHLSEDIQDEIKSISKQISELSIDFNRNLNEENTVLLFSKEDLVGLAESYANGLEKTEDGQYKVTLAYPHYFPLMKRCHVPETRRKMEKAFHSRCKDVNTAILEKLIELRARLANLLGFSSHANYMLEMTMAKNSENVARFLDELYERLRPVGEQERHYLLSLKDADCERRGLEFDGQLHAWDMPYYMNQVEQVKFAVDKDKLIEYFPLQVVTEGLLDIYQDLLGLKFQQVEKPHVWHDSVKLYSVLDSVTGNQIGQFYLDLHPREGKYSHAACFGLQPGCVGPDGERRMPVAAMVANFTKPTSTWPSLLQHHEVETFFHEFGHIMHELCSRSRYADFSGTQVETDFVEVPSQMLENWVWEKEPLRRMSRHYKDDSPIPDALLDKLIASRVANTGLMNLRQIVLSKADQTLHTKDRADTAAEFAKLSKDILGIPATPGTNMTASFSHLAGGYDGQYYSYLWSEVYSMDIFFTRFKKEGILNPKVGREYRRVVLEAGGSVDGMEMLKTFLGREPCQDAFFQCKGLTQSVTEQNLH

Target ORF information:

RefSeq Version	XM_017357959.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neurolysin (metallopeptidase M3 family) (nln), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357959.1

ORF Insert Sequence:

ATGACACGGT CGGGTCAGTG GATATTGGAA AGGTGTGCTT TGAGAACACT CTGCAGGTCA 
TTGCAGACGC CAAAGCAGAT TATGCTGGAA AAACAGTCCT GTGATTTACT GCCTGAGTCA 
GAGAGATTCC TGGGACGACT TATAAAGTTA GGAAAACGGA ATGGATTACA TTTGTCTGAG 
GATATACAAG ATGAAATTAA GTCAATTTCT AAGCAAATAA GTGAACTCTC CATAGATTTC 
AACCGAAATC TGAATGAAGA AAATACAGTG CTTTTGTTCT CGAAAGAAGA TCTTGTTGGT 
CTTGCCGAAA GCTACGCGAA CGGCCTGGAA AAAACGGAAG ATGGACAGTA CAAAGTTACC 
CTCGCGTATC CTCACTACTT TCCTTTAATG AAAAGATGTC ATGTCCCTGA AACCAGGAGG 
AAGATGGAGA AGGCCTTTCA TAGCAGATGC AAAGATGTGA ACACTGCTAT TCTAGAAAAG 
CTTATTGAGC TGCGGGCAAG GCTAGCCAAT CTACTGGGCT TCAGCAGTCA TGCCAATTAC 
ATGCTGGAAA TGACCATGGC CAAAAACTCA GAGAACGTGG CCAGATTTCT TGATGAGTTG 
TACGAGAGAT TGAGGCCAGT GGGCGAGCAG GAGAGGCATT ACCTGCTGTC ACTCAAAGAT 
GCAGACTGTG AGAGGCGGGG CTTGGAGTTT GACGGACAGC TCCATGCGTG GGACATGCCC 
TACTACATGA ACCAGGTGGA GCAAGTGAAG TTTGCGGTGG ACAAAGACAA ACTGATCGAG 
TACTTCCCTC TGCAGGTGGT GACTGAGGGC CTGCTGGATA TATATCAGGA TCTGTTGGGT 
CTAAAGTTCC AGCAAGTGGA AAAACCTCAT GTGTGGCACG ACAGCGTCAA ACTCTACTCT 
GTACTCGACT CTGTGACTGG AAATCAGATT GGACAGTTTT ACTTGGACCT ACATCCTAGG 
GAGGGAAAGT ACAGCCACGC TGCATGCTTT GGGCTCCAGC CAGGGTGTGT TGGGCCTGAT 
GGGGAACGTA GGATGCCTGT GGCCGCCATG GTGGCCAACT TCACCAAGCC CACCAGCACC 
TGGCCTTCGC TTCTGCAGCA CCACGAGGTG GAGACCTTCT TCCACGAGTT TGGACACATC 
ATGCATGAGC TCTGCTCCAG GAGCCGATAT GCAGACTTCA GTGGGACACA GGTGGAGACA 
GATTTTGTGG AGGTGCCGTC TCAGATGCTG GAGAACTGGG TTTGGGAAAA GGAGCCTCTC 
AGAAGGATGT CCCGTCACTA TAAAGATGAC AGCCCGATCC CAGATGCTCT GCTGGACAAA 
CTCATCGCAT CTAGAGTGGC CAACACAGGT CTGATGAACC TCAGGCAGAT AGTGCTCAGT 
AAAGCTGACC AAACGCTCCA CACTAAAGAC CGAGCAGACA CTGCAGCAGA GTTTGCCAAG 
CTCAGCAAAG ACATCCTGGG AATCCCTGCC ACACCAGGCA CAAACATGAC GGCTAGTTTC 
AGTCATCTGG CCGGAGGTTA TGACGGTCAG TACTACAGTT ACCTCTGGAG TGAAGTCTAC 
TCCATGGACA TCTTTTTCAC TCGCTTCAAA AAGGAAGGAA TCCTGAATCC AAAGGTGGGC 
AGAGAATACA GACGTGTGGT TCTGGAAGCA GGAGGTTCAG TGGATGGCAT GGAAATGCTG 
AAAACGTTCC TGGGACGTGA ACCGTGCCAG GATGCATTTT TTCAGTGTAA AGGACTCACA 
CAATCAGTTA CAGAACAAAA CTTGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nln ( XM_017357959.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213448.1 Danio rerio neurolysin (metallopeptidase M3 family) (nln), transcript variant X3, mRNA.

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CloneID	ODa54019
Clone ID Related Accession (Same CDS sequence)	XM_017357959.1 , XM_017357959.2
Accession Version	XM_017357959.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neurolysin, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017357959.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACGGT CGGGTCAGTG GATATTGGAA AGGTGTGCTT TGAGAACACT CTGCAGGTCA 
TTGCAGACGC CAAAGCAGAT TATGCTGGAA AAACAGTCCT GTGATTTACT GCCTGAGTCA 
GAGAGATTCC TGGGACGACT TATAAAGTTA GGAAAACGGA ATGGATTACA TTTGTCTGAG 
GATATACAAG ATGAAATTAA GTCAATTTCT AAGCAAATAA GTGAACTCTC CATAGATTTC 
AACCGAAATC TGAATGAAGA AAATACAGTG CTTTTGTTCT CGAAAGAAGA TCTTGTTGGT 
CTTGCCGAAA GCTACGCGAA CGGCCTGGAA AAAACGGAAG ATGGACAGTA CAAAGTTACC 
CTCGCGTATC CTCACTACTT TCCTTTAATG AAAAGATGTC ATGTCCCTGA AACCAGGAGG 
AAGATGGAGA AGGCCTTTCA TAGCAGATGC AAAGATGTGA ACACTGCTAT TCTAGAAAAG 
CTTATTGAGC TGCGGGCAAG GCTAGCCAAT CTACTGGGCT TCAGCAGTCA TGCCAATTAC 
ATGCTGGAAA TGACCATGGC CAAAAACTCA GAGAACGTGG CCAGATTTCT TGATGAGTTG 
TACGAGAGAT TGAGGCCAGT GGGCGAGCAG GAGAGGCATT ACCTGCTGTC ACTCAAAGAT 
GCAGACTGTG AGAGGCGGGG CTTGGAGTTT GACGGACAGC TCCATGCGTG GGACATGCCC 
TACTACATGA ACCAGGTGGA GCAAGTGAAG TTTGCGGTGG ACAAAGACAA ACTGATCGAG 
TACTTCCCTC TGCAGGTGGT GACTGAGGGC CTGCTGGATA TATATCAGGA TCTGTTGGGT 
CTAAAGTTCC AGCAAGTGGA AAAACCTCAT GTGTGGCACG ACAGCGTCAA ACTCTACTCT 
GTACTCGACT CTGTGACTGG AAATCAGATT GGACAGTTTT ACTTGGACCT ACATCCTAGG 
GAGGGAAAGT ACAGCCACGC TGCATGCTTT GGGCTCCAGC CAGGGTGTGT TGGGCCTGAT 
GGGGAACGTA GGATGCCTGT GGCCGCCATG GTGGCCAACT TCACCAAGCC CACCAGCACC 
TGGCCTTCGC TTCTGCAGCA CCACGAGGTG GAGACCTTCT TCCACGAGTT TGGACACATC 
ATGCATGAGC TCTGCTCCAG GAGCCGATAT GCAGACTTCA GTGGGACACA GGTGGAGACA 
GATTTTGTGG AGGTGCCGTC TCAGATGCTG GAGAACTGGG TTTGGGAAAA GGAGCCTCTC 
AGAAGGATGT CCCGTCACTA TAAAGATGAC AGCCCGATCC CAGATGCTCT GCTGGACAAA 
CTCATCGCAT CTAGAGTGGC CAACACAGGT CTGATGAACC TCAGGCAGAT AGTGCTCAGT 
AAAGCTGACC AAACGCTCCA CACTAAAGAC CGAGCAGACA CTGCAGCAGA GTTTGCCAAG 
CTCAGCAAAG ACATCCTGGG AATCCCTGCC ACACCAGGCA CAAACATGAC GGCTAGTTTC 
AGTCATCTGG CCGGAGGTTA TGACGGTCAG TACTACAGTT ACCTCTGGAG TGAAGTCTAC 
TCCATGGACA TCTTTTTCAC TCGCTTCAAA AAGGAAGGAA TCCTGAATCC AAAGGTGGGC 
AGAGAATACA GACGTGTGGT TCTGGAAGCA GGAGGTTCAG TGGATGGCAT GGAAATGCTG 
AAAACGTTCC TGGGACGTGA ACCGTGCCAG GATGCATTTT TTCAGTGTAA AGGACTCACA 
CAATCAGTTA CAGAACAAAA CTTGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213448.1
CDS	174..1943
Translation	MTRSGQWILERCALRTLCRSLQTPKQIMLEKQSCDLLPESERFLGRLIKLGKRNGLHLSEDIQDEIKSISKQISELSIDFNRNLNEENTVLLFSKEDLVGLAESYANGLEKTEDGQYKVTLAYPHYFPLMKRCHVPETRRKMEKAFHSRCKDVNTAILEKLIELRARLANLLGFSSHANYMLEMTMAKNSENVARFLDELYERLRPVGEQERHYLLSLKDADCERRGLEFDGQLHAWDMPYYMNQVEQVKFAVDKDKLIEYFPLQVVTEGLLDIYQDLLGLKFQQVEKPHVWHDSVKLYSVLDSVTGNQIGQFYLDLHPREGKYSHAACFGLQPGCVGPDGERRMPVAAMVANFTKPTSTWPSLLQHHEVETFFHEFGHIMHELCSRSRYADFSGTQVETDFVEVPSQMLENWVWEKEPLRRMSRHYKDDSPIPDALLDKLIASRVANTGLMNLRQIVLSKADQTLHTKDRADTAAEFAKLSKDILGIPATPGTNMTASFSHLAGGYDGQYYSYLWSEVYSMDIFFTRFKKEGILNPKVGREYRRVVLEAGGSVDGMEMLKTFLGREPCQDAFFQCKGLTQSVTEQNLH

Target ORF information:

RefSeq Version	XM_017357959.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neurolysin (metallopeptidase M3 family) (nln), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357959.2

ORF Insert Sequence:

ATGACACGGT CGGGTCAGTG GATATTGGAA AGGTGTGCTT TGAGAACACT CTGCAGGTCA 
TTGCAGACGC CAAAGCAGAT TATGCTGGAA AAACAGTCCT GTGATTTACT GCCTGAGTCA 
GAGAGATTCC TGGGACGACT TATAAAGTTA GGAAAACGGA ATGGATTACA TTTGTCTGAG 
GATATACAAG ATGAAATTAA GTCAATTTCT AAGCAAATAA GTGAACTCTC CATAGATTTC 
AACCGAAATC TGAATGAAGA AAATACAGTG CTTTTGTTCT CGAAAGAAGA TCTTGTTGGT 
CTTGCCGAAA GCTACGCGAA CGGCCTGGAA AAAACGGAAG ATGGACAGTA CAAAGTTACC 
CTCGCGTATC CTCACTACTT TCCTTTAATG AAAAGATGTC ATGTCCCTGA AACCAGGAGG 
AAGATGGAGA AGGCCTTTCA TAGCAGATGC AAAGATGTGA ACACTGCTAT TCTAGAAAAG 
CTTATTGAGC TGCGGGCAAG GCTAGCCAAT CTACTGGGCT TCAGCAGTCA TGCCAATTAC 
ATGCTGGAAA TGACCATGGC CAAAAACTCA GAGAACGTGG CCAGATTTCT TGATGAGTTG 
TACGAGAGAT TGAGGCCAGT GGGCGAGCAG GAGAGGCATT ACCTGCTGTC ACTCAAAGAT 
GCAGACTGTG AGAGGCGGGG CTTGGAGTTT GACGGACAGC TCCATGCGTG GGACATGCCC 
TACTACATGA ACCAGGTGGA GCAAGTGAAG TTTGCGGTGG ACAAAGACAA ACTGATCGAG 
TACTTCCCTC TGCAGGTGGT GACTGAGGGC CTGCTGGATA TATATCAGGA TCTGTTGGGT 
CTAAAGTTCC AGCAAGTGGA AAAACCTCAT GTGTGGCACG ACAGCGTCAA ACTCTACTCT 
GTACTCGACT CTGTGACTGG AAATCAGATT GGACAGTTTT ACTTGGACCT ACATCCTAGG 
GAGGGAAAGT ACAGCCACGC TGCATGCTTT GGGCTCCAGC CAGGGTGTGT TGGGCCTGAT 
GGGGAACGTA GGATGCCTGT GGCCGCCATG GTGGCCAACT TCACCAAGCC CACCAGCACC 
TGGCCTTCGC TTCTGCAGCA CCACGAGGTG GAGACCTTCT TCCACGAGTT TGGACACATC 
ATGCATGAGC TCTGCTCCAG GAGCCGATAT GCAGACTTCA GTGGGACACA GGTGGAGACA 
GATTTTGTGG AGGTGCCGTC TCAGATGCTG GAGAACTGGG TTTGGGAAAA GGAGCCTCTC 
AGAAGGATGT CCCGTCACTA TAAAGATGAC AGCCCGATCC CAGATGCTCT GCTGGACAAA 
CTCATCGCAT CTAGAGTGGC CAACACAGGT CTGATGAACC TCAGGCAGAT AGTGCTCAGT 
AAAGCTGACC AAACGCTCCA CACTAAAGAC CGAGCAGACA CTGCAGCAGA GTTTGCCAAG 
CTCAGCAAAG ACATCCTGGG AATCCCTGCC ACACCAGGCA CAAACATGAC GGCTAGTTTC 
AGTCATCTGG CCGGAGGTTA TGACGGTCAG TACTACAGTT ACCTCTGGAG TGAAGTCTAC 
TCCATGGACA TCTTTTTCAC TCGCTTCAAA AAGGAAGGAA TCCTGAATCC AAAGGTGGGC 
AGAGAATACA GACGTGTGGT TCTGGAAGCA GGAGGTTCAG TGGATGGCAT GGAAATGCTG 
AAAACGTTCC TGGGACGTGA ACCGTGCCAG GATGCATTTT TTCAGTGTAA AGGACTCACA 
CAATCAGTTA CAGAACAAAA CTTGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

A reference growth curve for nutritional experiments in zebrafish (Danio rerio) and changes in whole body proteome during development.
Fish physiology and biochemistry36(4)1199-215(2010 Dec)
G?mez-Requeni P,Concei??o LE,Olderbakk Jordal AE,R?nnestad I

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nln cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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