LOC100331775 cDNA ORF clone, Danio rerio(Zebrafish)

The following LOC100331775 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100331775 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa55478 XM_009292173.3
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Danio rerio ubiquitin conjugating enzyme E2 O (ube2o), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
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ODa55478 XM_009292173.2 Danio rerio (E3-independent) E2 ubiquitin-conjugating enzyme UBE2O (LOC100331775), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa55478
    Clone ID Related Accession (Same CDS sequence) XM_009292173.3 , XM_009292173.2
    Accession Version XM_009292173.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3918bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product (E3-independent) E2 ubiquitin-conjugating enzyme
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) and transcript sequence (GDQH01036467.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009292173.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 975 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGCAAT TCGCCCCAGC AGCGCTTTCT CCGGCCGTAA GCCTGAGCTC CGAGCCTCCG 
    TCCAGCGCGT TCTCTCCCAC GGAAGGTGGA ACACAGCGGC TCCTGTTTAC GCATGATCTG
    GTCTCCGGAA AACATCACGG GGCGATGCGT TTTGGGCTTG TACGGATGAT TCATGGAGAG
    GAGGATTTTG AAGATTCGGA CTCTGAGATT GGTGGTGATG GCGGTGGAGG AGGTGGAGGC
    GCTCCCGCTA ACTCGGATAA CGAGAGTGGT GCTGCGGAAA CACGGCCATG GCCTCTCGGT
    AGAGGATTTG TGCGGGTTCA GTGGTATCCT GAAGGAACGA AACAGGATGT TCGAGAAACG
    AAGCTGAAAC TTGAAGATCG ATCTATTGTG CCTAGAGACT TTGTCCGGCG GATAAATTCA
    AATGAAAAAC AGTGTGGAAT TGTGACTAAC ATAAACACAG AATGTGCAGT GAAACTTGTA
    GGGACCAACT GTGCTCTATA TCCTGTAAAT AGCAAAGACC TGCAGCACAT CTGGTCTTAT
    ATGTATGGTG ACTACATCGT ATATGACTTC TGGCTTGGAA AGGTGTATGA TCTCAGCATC
    CAAATAGTTG TGAAGCTTTC CAATGGTGCA CGATGTTCTA TGAGTGAGGA AGATGGAGCA
    AAATTGTATG ATATTTACCC TCACATCAGT GATGTGGGTT TGTTCTTTGA TGATGCCTAT
    GGGTTCTATC CAGGTCAGGT TTTGATTGGT CCTGCAAAGG TGTTTGCAAA TGTGCAGTGG
    CTTTATGGTG TGAAACCAGT TCTCAGTAAG AAAAGCAAGT TCCGAGTTGT TGTGGAGGAG
    GTTCAAGTAG TGGAGCTCAA GGTAACATGG ATTACGAAAA GTTACTCTCC AAAGGGTTCA
    GACAGTGTCT TCCCACCACC TTCGACTATC ACACAGGAGA ACCTTTCCAG GGTAAAGCGA
    CTGGGCTACT TTGATCATAC ACAAAGGCAG CTTGGAGAGA GAGCCCTGTA TGTTTTTCCT
    GCCAAGAGTG AAACCACAAG GATCACTTGT GAGGGACCAG AAGGGACACC TGCTCATCAT
    GATGACCCTG TTATTCGAAA GTTAAAAAGA ATGTTTAGAA AGGAAGCTGG AAAGAGAGTT
    ACCGAAAATG CAGATACACT GAACATCAAT TTACAGCCAC TTGAGAAAAA GGACAGCAAA
    GGTAAAGAAG AATCAGTGCA AGAACAGGAC TGTTCTGGTA TTAATTCCAT TCCATGCTAC
    TCCAACAATG ATCCTGATCA TAATTATCTG GAAGAGGGGA AAGAGCCATC ACCTGATGCT
    TCTCAGGAAC AATGGGCCCA TTTGGGGGAC CCAGATTCAG ATGAGGAGCC AGGGGATAAC
    ACTGATGATA CCAGCTCTGT TACTTCTTCT GCTAGCTCTA CAGCCTCACT TCAAAGTGGC
    CCAACACGAA AAAAAAGCAT TCCTCTATCT ATTCGCAACT TAAAGAGAAA GCACAAGAAG
    AAGAGGACAA AATTTTCTCG TGAATTTAAA CCAGGAGACA GGGTGGCAGT TGAAGTTGTC
    TCCACTGTAA CTTCTGCAGA TGTGATGTGG AAGGATGGTA AAATAGAGAC AGGGATCCGC
    TCCAATGATT TAATCCCTAT CCAGCACCTT GACAACCATG AGTTCTGCCC TGGAGATTAT
    GTTGTAGACA AGCGGCCCCA GGTTCTTCAA GATCCAGCCA TCTACGGGGT GATTCAGTCA
    GGTGATCATA AAGCCAGAAC ATGTGCCGTC AAGTGGGTTA AACTCAATGC TGCAGGTGAT
    GATGTTGAGG TGATTGGGGA GGAAGACGAT GTTAGTGTTT ATGACATTGC TGATCATCCA
    GATTTCCATT TTCGTACAAC GGATATTATC ATAAGGATTG GCAATTCTGG TACAGAGGAC
    TGCGGAACTG AGAGATCTGT TGGCCAAGTC TGCAGGGTGG ATTTAAGCAG TAAAGTGGAA
    GTTGTTTGGG CTGATAATTC AAAGACAATT GTCTTGCCAC AGCACTTGTA TAATGTTGAG
    TCTGAAATTG AAGAAACATA TTATGACTCT GCCGAGGGCA GCAGTAGTGG TGCCTCATCT
    GAAGAATGGG AAGATGAGAG TGATAGTTGG GAAACAGATA ATGGACAAGT GGAAGAAGAA
    CCAGGGGGTG AAGAAACCAT CTCCATTTCC TCTTCCACCA CAGCTACCAC ATCTGATGAA
    AAAACTAATG GCATACTTGC CTTCAAAACT ACAGACTCAA CAGGAACATT TTCTACAACT
    GAACTTGCTG GAGGTGGTGT TGCTACACCT GAGGAAAAGC CCACTAAGGA AGCTCCACCA
    AGGGGATTTC GTGAGCTTAA AGAAGCCCTA AAGATTTTGG AAAGTTTAAA GAACATGACT
    GTTGAGCAGC TCTGGACTGG ATCACCAACC TCTCCCACAG CAGAACCATC AGCTGCAACT
    GCTGCCACAG CTTCTGCTGA TATCAAACCC ACAAAGGAAA AACGCTTTCT GGATGACATT
    AAGAAACTTC AGGAGAATTT GAAAAAGACT CTAGACAATG TTGCCATTGT TGAAGAAGAT
    AAAATGGAGG CTCCCGTAGA AGAGGAAAGG ATTATCACTC CAGTGTTAAC TGAACCACAG
    ACACCTGTGC GTTCTGAATG GCCTAGTGAC ACTCCAGTTT TGTGCCAGCA GAGTGGAGGC
    AAACCAGGTG TCACCTTTAC CAGTGCTAAA GGGGAAGTGT TTTCTGTTTT GGAATGGGTA
    CCAGACACCC ACTCATTTAA AAAAATGGAA TTTCAGCCAG CAGAGGCAAA GAAGTTCTTT
    AGCACTGTAA GGAAAGAAAT GGCCTTGCTG GCCACCTCTC TGCCTGATGG TATCATGGTG
    AAAACCTTTG AGGACAGAAT GGACCTTTTT TCAGCTCTCA TTAAGGGCCC TCACCGCACT
    CCCTACGAGG ATGGACTCTT TCTCTTTGAT ATCCAGCTTC CTAATATCTA TCCTGCTGTT
    CCTCCTCTTT TCCGTTATTT GTCTCAGTGT AGCGGGCGGC TTAATCCTAA CCTTTATGAT
    AATGGAAAGG TTTGTGTCAG CCTTCTTGGC ACCTGGATTG GAAAGGGTAC TGAAAGATGG
    ACAAGCAAAT CAAGTTTACT TCAAGTGCTT ATCTCTATAC AAGGTCTGAT TCTGGTCAAT
    GAGCCATATT ACAATGAGGC TGGCTTTGAC AGTGACCGGG GACTTCAGGA AGGTTATGAA
    AATAGTCGCT GTTACAACGA GATGGCACTC ATTAAGCTGG TACAGTCAAT GACTCAGCTT
    CTTCAGCAGC CTGTAGAGGT CTTTCAGCAG GAAGTCTATG AACACTTTGC CTGCAGTGGT
    TGGCGGCTGG TCCATCGGCT GGAGTCCTGG CTAGAGTTAG ATGGGGCAAT CACAGATCAT
    GCTCCACGAT GTGGTGTTGG AAGCCTCTAT GCAGAACCTC TAGAAGAGAT CCCCTCAGTT
    CTTTACCACT GCAGTTCTAG CAAGCAGGAC ATACAAGTCG AAGAAGATTT TGAGGATTCG
    GGACTCAGTC TCTCTACAAT GGCAACCAGT GGACCTACTG GTTTAACTGA AAAAGATTTG
    AGCCAGAACT CTGAGTGTGA TAGTTCATTG GTGGGTGGTG CAGGATCAGA AATACCAGAA
    GTTCCTGAAG TAATGTCAAA TTTTTTGGGA TCTCAACAAA GTGTAAAATC ATGTCAGGAG
    TCTCAACCAG CTGTGAGACC AAAGAAAAGG AGAAAGAGTT ATCGTAGTTT CCTTCCAGAG
    CGGAGTGGTT ATCCTGATAT TGGATTCCCC CTCTTCCCTC TTTCCAAAGG TTTTGTAAAG
    AGCATGTGTG TAGTTCTGCA GCATTATCGA GCTACTCTCA CAGCTGCTGG TGTTCCTGAA
    CACCCAGAAA ACAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290448.2
    CDS26..3943
    Translation

    Target ORF information:

    RefSeq Version XM_009292173.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ubiquitin conjugating enzyme E2 O (ube2o), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292173.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGCAAT TCGCCCCAGC AGCGCTTTCT CCGGCCGTAA GCCTGAGCTC CGAGCCTCCG 
    TCCAGCGCGT TCTCTCCCAC GGAAGGTGGA ACACAGCGGC TCCTGTTTAC GCATGATCTG
    GTCTCCGGAA AACATCACGG GGCGATGCGT TTTGGGCTTG TACGGATGAT TCATGGAGAG
    GAGGATTTTG AAGATTCGGA CTCTGAGATT GGTGGTGATG GCGGTGGAGG AGGTGGAGGC
    GCTCCCGCTA ACTCGGATAA CGAGAGTGGT GCTGCGGAAA CACGGCCATG GCCTCTCGGT
    AGAGGATTTG TGCGGGTTCA GTGGTATCCT GAAGGAACGA AACAGGATGT TCGAGAAACG
    AAGCTGAAAC TTGAAGATCG ATCTATTGTG CCTAGAGACT TTGTCCGGCG GATAAATTCA
    AATGAAAAAC AGTGTGGAAT TGTGACTAAC ATAAACACAG AATGTGCAGT GAAACTTGTA
    GGGACCAACT GTGCTCTATA TCCTGTAAAT AGCAAAGACC TGCAGCACAT CTGGTCTTAT
    ATGTATGGTG ACTACATCGT ATATGACTTC TGGCTTGGAA AGGTGTATGA TCTCAGCATC
    CAAATAGTTG TGAAGCTTTC CAATGGTGCA CGATGTTCTA TGAGTGAGGA AGATGGAGCA
    AAATTGTATG ATATTTACCC TCACATCAGT GATGTGGGTT TGTTCTTTGA TGATGCCTAT
    GGGTTCTATC CAGGTCAGGT TTTGATTGGT CCTGCAAAGG TGTTTGCAAA TGTGCAGTGG
    CTTTATGGTG TGAAACCAGT TCTCAGTAAG AAAAGCAAGT TCCGAGTTGT TGTGGAGGAG
    GTTCAAGTAG TGGAGCTCAA GGTAACATGG ATTACGAAAA GTTACTCTCC AAAGGGTTCA
    GACAGTGTCT TCCCACCACC TTCGACTATC ACACAGGAGA ACCTTTCCAG GGTAAAGCGA
    CTGGGCTACT TTGATCATAC ACAAAGGCAG CTTGGAGAGA GAGCCCTGTA TGTTTTTCCT
    GCCAAGAGTG AAACCACAAG GATCACTTGT GAGGGACCAG AAGGGACACC TGCTCATCAT
    GATGACCCTG TTATTCGAAA GTTAAAAAGA ATGTTTAGAA AGGAAGCTGG AAAGAGAGTT
    ACCGAAAATG CAGATACACT GAACATCAAT TTACAGCCAC TTGAGAAAAA GGACAGCAAA
    GGTAAAGAAG AATCAGTGCA AGAACAGGAC TGTTCTGGTA TTAATTCCAT TCCATGCTAC
    TCCAACAATG ATCCTGATCA TAATTATCTG GAAGAGGGGA AAGAGCCATC ACCTGATGCT
    TCTCAGGAAC AATGGGCCCA TTTGGGGGAC CCAGATTCAG ATGAGGAGCC AGGGGATAAC
    ACTGATGATA CCAGCTCTGT TACTTCTTCT GCTAGCTCTA CAGCCTCACT TCAAAGTGGC
    CCAACACGAA AAAAAAGCAT TCCTCTATCT ATTCGCAACT TAAAGAGAAA GCACAAGAAG
    AAGAGGACAA AATTTTCTCG TGAATTTAAA CCAGGAGACA GGGTGGCAGT TGAAGTTGTC
    TCCACTGTAA CTTCTGCAGA TGTGATGTGG AAGGATGGTA AAATAGAGAC AGGGATCCGC
    TCCAATGATT TAATCCCTAT CCAGCACCTT GACAACCATG AGTTCTGCCC TGGAGATTAT
    GTTGTAGACA AGCGGCCCCA GGTTCTTCAA GATCCAGCCA TCTACGGGGT GATTCAGTCA
    GGTGATCATA AAGCCAGAAC ATGTGCCGTC AAGTGGGTTA AACTCAATGC TGCAGGTGAT
    GATGTTGAGG TGATTGGGGA GGAAGACGAT GTTAGTGTTT ATGACATTGC TGATCATCCA
    GATTTCCATT TTCGTACAAC GGATATTATC ATAAGGATTG GCAATTCTGG TACAGAGGAC
    TGCGGAACTG AGAGATCTGT TGGCCAAGTC TGCAGGGTGG ATTTAAGCAG TAAAGTGGAA
    GTTGTTTGGG CTGATAATTC AAAGACAATT GTCTTGCCAC AGCACTTGTA TAATGTTGAG
    TCTGAAATTG AAGAAACATA TTATGACTCT GCCGAGGGCA GCAGTAGTGG TGCCTCATCT
    GAAGAATGGG AAGATGAGAG TGATAGTTGG GAAACAGATA ATGGACAAGT GGAAGAAGAA
    CCAGGGGGTG AAGAAACCAT CTCCATTTCC TCTTCCACCA CAGCTACCAC ATCTGATGAA
    AAAACTAATG GCATACTTGC CTTCAAAACT ACAGACTCAA CAGGAACATT TTCTACAACT
    GAACTTGCTG GAGGTGGTGT TGCTACACCT GAGGAAAAGC CCACTAAGGA AGCTCCACCA
    AGGGGATTTC GTGAGCTTAA AGAAGCCCTA AAGATTTTGG AAAGTTTAAA GAACATGACT
    GTTGAGCAGC TCTGGACTGG ATCACCAACC TCTCCCACAG CAGAACCATC AGCTGCAACT
    GCTGCCACAG CTTCTGCTGA TATCAAACCC ACAAAGGAAA AACGCTTTCT GGATGACATT
    AAGAAACTTC AGGAGAATTT GAAAAAGACT CTAGACAATG TTGCCATTGT TGAAGAAGAT
    AAAATGGAGG CTCCCGTAGA AGAGGAAAGG ATTATCACTC CAGTGTTAAC TGAACCACAG
    ACACCTGTGC GTTCTGAATG GCCTAGTGAC ACTCCAGTTT TGTGCCAGCA GAGTGGAGGC
    AAACCAGGTG TCACCTTTAC CAGTGCTAAA GGGGAAGTGT TTTCTGTTTT GGAATGGGTA
    CCAGACACCC ACTCATTTAA AAAAATGGAA TTTCAGCCAG CAGAGGCAAA GAAGTTCTTT
    AGCACTGTAA GGAAAGAAAT GGCCTTGCTG GCCACCTCTC TGCCTGATGG TATCATGGTG
    AAAACCTTTG AGGACAGAAT GGACCTTTTT TCAGCTCTCA TTAAGGGCCC TCACCGCACT
    CCCTACGAGG ATGGACTCTT TCTCTTTGAT ATCCAGCTTC CTAATATCTA TCCTGCTGTT
    CCTCCTCTTT TCCGTTATTT GTCTCAGTGT AGCGGGCGGC TTAATCCTAA CCTTTATGAT
    AATGGAAAGG TTTGTGTCAG CCTTCTTGGC ACCTGGATTG GAAAGGGTAC TGAAAGATGG
    ACAAGCAAAT CAAGTTTACT TCAAGTGCTT ATCTCTATAC AAGGTCTGAT TCTGGTCAAT
    GAGCCATATT ACAATGAGGC TGGCTTTGAC AGTGACCGGG GACTTCAGGA AGGTTATGAA
    AATAGTCGCT GTTACAACGA GATGGCACTC ATTAAGCTGG TACAGTCAAT GACTCAGCTT
    CTTCAGCAGC CTGTAGAGGT CTTTCAGCAG GAAGTCTATG AACACTTTGC CTGCAGTGGT
    TGGCGGCTGG TCCATCGGCT GGAGTCCTGG CTAGAGTTAG ATGGGGCAAT CACAGATCAT
    GCTCCACGAT GTGGTGTTGG AAGCCTCTAT GCAGAACCTC TAGAAGAGAT CCCCTCAGTT
    CTTTACCACT GCAGTTCTAG CAAGCAGGAC ATACAAGTCG AAGAAGATTT TGAGGATTCG
    GGACTCAGTC TCTCTACAAT GGCAACCAGT GGACCTACTG GTTTAACTGA AAAAGATTTG
    AGCCAGAACT CTGAGTGTGA TAGTTCATTG GTGGGTGGTG CAGGATCAGA AATACCAGAA
    GTTCCTGAAG TAATGTCAAA TTTTTTGGGA TCTCAACAAA GTGTAAAATC ATGTCAGGAG
    TCTCAACCAG CTGTGAGACC AAAGAAAAGG AGAAAGAGTT ATCGTAGTTT CCTTCCAGAG
    CGGAGTGGTT ATCCTGATAT TGGATTCCCC CTCTTCCCTC TTTCCAAAGG TTTTGTAAAG
    AGCATGTGTG TAGTTCTGCA GCATTATCGA GCTACTCTCA CAGCTGCTGG TGTTCCTGAA
    CACCCAGAAA ACAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55478
    Clone ID Related Accession (Same CDS sequence) XM_009292173.3 , XM_009292173.2
    Accession Version XM_009292173.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3918bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product (E3-independent) E2 ubiquitin-conjugating enzyme UBE2O
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) and transcript sequence (GDQH01036467.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292173.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 975 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGCAAT TCGCCCCAGC AGCGCTTTCT CCGGCCGTAA GCCTGAGCTC CGAGCCTCCG 
    TCCAGCGCGT TCTCTCCCAC GGAAGGTGGA ACACAGCGGC TCCTGTTTAC GCATGATCTG
    GTCTCCGGAA AACATCACGG GGCGATGCGT TTTGGGCTTG TACGGATGAT TCATGGAGAG
    GAGGATTTTG AAGATTCGGA CTCTGAGATT GGTGGTGATG GCGGTGGAGG AGGTGGAGGC
    GCTCCCGCTA ACTCGGATAA CGAGAGTGGT GCTGCGGAAA CACGGCCATG GCCTCTCGGT
    AGAGGATTTG TGCGGGTTCA GTGGTATCCT GAAGGAACGA AACAGGATGT TCGAGAAACG
    AAGCTGAAAC TTGAAGATCG ATCTATTGTG CCTAGAGACT TTGTCCGGCG GATAAATTCA
    AATGAAAAAC AGTGTGGAAT TGTGACTAAC ATAAACACAG AATGTGCAGT GAAACTTGTA
    GGGACCAACT GTGCTCTATA TCCTGTAAAT AGCAAAGACC TGCAGCACAT CTGGTCTTAT
    ATGTATGGTG ACTACATCGT ATATGACTTC TGGCTTGGAA AGGTGTATGA TCTCAGCATC
    CAAATAGTTG TGAAGCTTTC CAATGGTGCA CGATGTTCTA TGAGTGAGGA AGATGGAGCA
    AAATTGTATG ATATTTACCC TCACATCAGT GATGTGGGTT TGTTCTTTGA TGATGCCTAT
    GGGTTCTATC CAGGTCAGGT TTTGATTGGT CCTGCAAAGG TGTTTGCAAA TGTGCAGTGG
    CTTTATGGTG TGAAACCAGT TCTCAGTAAG AAAAGCAAGT TCCGAGTTGT TGTGGAGGAG
    GTTCAAGTAG TGGAGCTCAA GGTAACATGG ATTACGAAAA GTTACTCTCC AAAGGGTTCA
    GACAGTGTCT TCCCACCACC TTCGACTATC ACACAGGAGA ACCTTTCCAG GGTAAAGCGA
    CTGGGCTACT TTGATCATAC ACAAAGGCAG CTTGGAGAGA GAGCCCTGTA TGTTTTTCCT
    GCCAAGAGTG AAACCACAAG GATCACTTGT GAGGGACCAG AAGGGACACC TGCTCATCAT
    GATGACCCTG TTATTCGAAA GTTAAAAAGA ATGTTTAGAA AGGAAGCTGG AAAGAGAGTT
    ACCGAAAATG CAGATACACT GAACATCAAT TTACAGCCAC TTGAGAAAAA GGACAGCAAA
    GGTAAAGAAG AATCAGTGCA AGAACAGGAC TGTTCTGGTA TTAATTCCAT TCCATGCTAC
    TCCAACAATG ATCCTGATCA TAATTATCTG GAAGAGGGGA AAGAGCCATC ACCTGATGCT
    TCTCAGGAAC AATGGGCCCA TTTGGGGGAC CCAGATTCAG ATGAGGAGCC AGGGGATAAC
    ACTGATGATA CCAGCTCTGT TACTTCTTCT GCTAGCTCTA CAGCCTCACT TCAAAGTGGC
    CCAACACGAA AAAAAAGCAT TCCTCTATCT ATTCGCAACT TAAAGAGAAA GCACAAGAAG
    AAGAGGACAA AATTTTCTCG TGAATTTAAA CCAGGAGACA GGGTGGCAGT TGAAGTTGTC
    TCCACTGTAA CTTCTGCAGA TGTGATGTGG AAGGATGGTA AAATAGAGAC AGGGATCCGC
    TCCAATGATT TAATCCCTAT CCAGCACCTT GACAACCATG AGTTCTGCCC TGGAGATTAT
    GTTGTAGACA AGCGGCCCCA GGTTCTTCAA GATCCAGCCA TCTACGGGGT GATTCAGTCA
    GGTGATCATA AAGCCAGAAC ATGTGCCGTC AAGTGGGTTA AACTCAATGC TGCAGGTGAT
    GATGTTGAGG TGATTGGGGA GGAAGACGAT GTTAGTGTTT ATGACATTGC TGATCATCCA
    GATTTCCATT TTCGTACAAC GGATATTATC ATAAGGATTG GCAATTCTGG TACAGAGGAC
    TGCGGAACTG AGAGATCTGT TGGCCAAGTC TGCAGGGTGG ATTTAAGCAG TAAAGTGGAA
    GTTGTTTGGG CTGATAATTC AAAGACAATT GTCTTGCCAC AGCACTTGTA TAATGTTGAG
    TCTGAAATTG AAGAAACATA TTATGACTCT GCCGAGGGCA GCAGTAGTGG TGCCTCATCT
    GAAGAATGGG AAGATGAGAG TGATAGTTGG GAAACAGATA ATGGACAAGT GGAAGAAGAA
    CCAGGGGGTG AAGAAACCAT CTCCATTTCC TCTTCCACCA CAGCTACCAC ATCTGATGAA
    AAAACTAATG GCATACTTGC CTTCAAAACT ACAGACTCAA CAGGAACATT TTCTACAACT
    GAACTTGCTG GAGGTGGTGT TGCTACACCT GAGGAAAAGC CCACTAAGGA AGCTCCACCA
    AGGGGATTTC GTGAGCTTAA AGAAGCCCTA AAGATTTTGG AAAGTTTAAA GAACATGACT
    GTTGAGCAGC TCTGGACTGG ATCACCAACC TCTCCCACAG CAGAACCATC AGCTGCAACT
    GCTGCCACAG CTTCTGCTGA TATCAAACCC ACAAAGGAAA AACGCTTTCT GGATGACATT
    AAGAAACTTC AGGAGAATTT GAAAAAGACT CTAGACAATG TTGCCATTGT TGAAGAAGAT
    AAAATGGAGG CTCCCGTAGA AGAGGAAAGG ATTATCACTC CAGTGTTAAC TGAACCACAG
    ACACCTGTGC GTTCTGAATG GCCTAGTGAC ACTCCAGTTT TGTGCCAGCA GAGTGGAGGC
    AAACCAGGTG TCACCTTTAC CAGTGCTAAA GGGGAAGTGT TTTCTGTTTT GGAATGGGTA
    CCAGACACCC ACTCATTTAA AAAAATGGAA TTTCAGCCAG CAGAGGCAAA GAAGTTCTTT
    AGCACTGTAA GGAAAGAAAT GGCCTTGCTG GCCACCTCTC TGCCTGATGG TATCATGGTG
    AAAACCTTTG AGGACAGAAT GGACCTTTTT TCAGCTCTCA TTAAGGGCCC TCACCGCACT
    CCCTACGAGG ATGGACTCTT TCTCTTTGAT ATCCAGCTTC CTAATATCTA TCCTGCTGTT
    CCTCCTCTTT TCCGTTATTT GTCTCAGTGT AGCGGGCGGC TTAATCCTAA CCTTTATGAT
    AATGGAAAGG TTTGTGTCAG CCTTCTTGGC ACCTGGATTG GAAAGGGTAC TGAAAGATGG
    ACAAGCAAAT CAAGTTTACT TCAAGTGCTT ATCTCTATAC AAGGTCTGAT TCTGGTCAAT
    GAGCCATATT ACAATGAGGC TGGCTTTGAC AGTGACCGGG GACTTCAGGA AGGTTATGAA
    AATAGTCGCT GTTACAACGA GATGGCACTC ATTAAGCTGG TACAGTCAAT GACTCAGCTT
    CTTCAGCAGC CTGTAGAGGT CTTTCAGCAG GAAGTCTATG AACACTTTGC CTGCAGTGGT
    TGGCGGCTGG TCCATCGGCT GGAGTCCTGG CTAGAGTTAG ATGGGGCAAT CACAGATCAT
    GCTCCACGAT GTGGTGTTGG AAGCCTCTAT GCAGAACCTC TAGAAGAGAT CCCCTCAGTT
    CTTTACCACT GCAGTTCTAG CAAGCAGGAC ATACAAGTCG AAGAAGATTT TGAGGATTCG
    GGACTCAGTC TCTCTACAAT GGCAACCAGT GGACCTACTG GTTTAACTGA AAAAGATTTG
    AGCCAGAACT CTGAGTGTGA TAGTTCATTG GTGGGTGGTG CAGGATCAGA AATACCAGAA
    GTTCCTGAAG TAATGTCAAA TTTTTTGGGA TCTCAACAAA GTGTAAAATC ATGTCAGGAG
    TCTCAACCAG CTGTGAGACC AAAGAAAAGG AGAAAGAGTT ATCGTAGTTT CCTTCCAGAG
    CGGAGTGGTT ATCCTGATAT TGGATTCCCC CTCTTCCCTC TTTCCAAAGG TTTTGTAAAG
    AGCATGTGTG TAGTTCTGCA GCATTATCGA GCTACTCTCA CAGCTGCTGG TGTTCCTGAA
    CACCCAGAAA ACAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290448.2
    CDS26..3943
    Translation

    Target ORF information:

    RefSeq Version XM_009292173.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio (E3-independent) E2 ubiquitin-conjugating enzyme UBE2O (LOC100331775), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292173.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGCAAT TCGCCCCAGC AGCGCTTTCT CCGGCCGTAA GCCTGAGCTC CGAGCCTCCG 
    TCCAGCGCGT TCTCTCCCAC GGAAGGTGGA ACACAGCGGC TCCTGTTTAC GCATGATCTG
    GTCTCCGGAA AACATCACGG GGCGATGCGT TTTGGGCTTG TACGGATGAT TCATGGAGAG
    GAGGATTTTG AAGATTCGGA CTCTGAGATT GGTGGTGATG GCGGTGGAGG AGGTGGAGGC
    GCTCCCGCTA ACTCGGATAA CGAGAGTGGT GCTGCGGAAA CACGGCCATG GCCTCTCGGT
    AGAGGATTTG TGCGGGTTCA GTGGTATCCT GAAGGAACGA AACAGGATGT TCGAGAAACG
    AAGCTGAAAC TTGAAGATCG ATCTATTGTG CCTAGAGACT TTGTCCGGCG GATAAATTCA
    AATGAAAAAC AGTGTGGAAT TGTGACTAAC ATAAACACAG AATGTGCAGT GAAACTTGTA
    GGGACCAACT GTGCTCTATA TCCTGTAAAT AGCAAAGACC TGCAGCACAT CTGGTCTTAT
    ATGTATGGTG ACTACATCGT ATATGACTTC TGGCTTGGAA AGGTGTATGA TCTCAGCATC
    CAAATAGTTG TGAAGCTTTC CAATGGTGCA CGATGTTCTA TGAGTGAGGA AGATGGAGCA
    AAATTGTATG ATATTTACCC TCACATCAGT GATGTGGGTT TGTTCTTTGA TGATGCCTAT
    GGGTTCTATC CAGGTCAGGT TTTGATTGGT CCTGCAAAGG TGTTTGCAAA TGTGCAGTGG
    CTTTATGGTG TGAAACCAGT TCTCAGTAAG AAAAGCAAGT TCCGAGTTGT TGTGGAGGAG
    GTTCAAGTAG TGGAGCTCAA GGTAACATGG ATTACGAAAA GTTACTCTCC AAAGGGTTCA
    GACAGTGTCT TCCCACCACC TTCGACTATC ACACAGGAGA ACCTTTCCAG GGTAAAGCGA
    CTGGGCTACT TTGATCATAC ACAAAGGCAG CTTGGAGAGA GAGCCCTGTA TGTTTTTCCT
    GCCAAGAGTG AAACCACAAG GATCACTTGT GAGGGACCAG AAGGGACACC TGCTCATCAT
    GATGACCCTG TTATTCGAAA GTTAAAAAGA ATGTTTAGAA AGGAAGCTGG AAAGAGAGTT
    ACCGAAAATG CAGATACACT GAACATCAAT TTACAGCCAC TTGAGAAAAA GGACAGCAAA
    GGTAAAGAAG AATCAGTGCA AGAACAGGAC TGTTCTGGTA TTAATTCCAT TCCATGCTAC
    TCCAACAATG ATCCTGATCA TAATTATCTG GAAGAGGGGA AAGAGCCATC ACCTGATGCT
    TCTCAGGAAC AATGGGCCCA TTTGGGGGAC CCAGATTCAG ATGAGGAGCC AGGGGATAAC
    ACTGATGATA CCAGCTCTGT TACTTCTTCT GCTAGCTCTA CAGCCTCACT TCAAAGTGGC
    CCAACACGAA AAAAAAGCAT TCCTCTATCT ATTCGCAACT TAAAGAGAAA GCACAAGAAG
    AAGAGGACAA AATTTTCTCG TGAATTTAAA CCAGGAGACA GGGTGGCAGT TGAAGTTGTC
    TCCACTGTAA CTTCTGCAGA TGTGATGTGG AAGGATGGTA AAATAGAGAC AGGGATCCGC
    TCCAATGATT TAATCCCTAT CCAGCACCTT GACAACCATG AGTTCTGCCC TGGAGATTAT
    GTTGTAGACA AGCGGCCCCA GGTTCTTCAA GATCCAGCCA TCTACGGGGT GATTCAGTCA
    GGTGATCATA AAGCCAGAAC ATGTGCCGTC AAGTGGGTTA AACTCAATGC TGCAGGTGAT
    GATGTTGAGG TGATTGGGGA GGAAGACGAT GTTAGTGTTT ATGACATTGC TGATCATCCA
    GATTTCCATT TTCGTACAAC GGATATTATC ATAAGGATTG GCAATTCTGG TACAGAGGAC
    TGCGGAACTG AGAGATCTGT TGGCCAAGTC TGCAGGGTGG ATTTAAGCAG TAAAGTGGAA
    GTTGTTTGGG CTGATAATTC AAAGACAATT GTCTTGCCAC AGCACTTGTA TAATGTTGAG
    TCTGAAATTG AAGAAACATA TTATGACTCT GCCGAGGGCA GCAGTAGTGG TGCCTCATCT
    GAAGAATGGG AAGATGAGAG TGATAGTTGG GAAACAGATA ATGGACAAGT GGAAGAAGAA
    CCAGGGGGTG AAGAAACCAT CTCCATTTCC TCTTCCACCA CAGCTACCAC ATCTGATGAA
    AAAACTAATG GCATACTTGC CTTCAAAACT ACAGACTCAA CAGGAACATT TTCTACAACT
    GAACTTGCTG GAGGTGGTGT TGCTACACCT GAGGAAAAGC CCACTAAGGA AGCTCCACCA
    AGGGGATTTC GTGAGCTTAA AGAAGCCCTA AAGATTTTGG AAAGTTTAAA GAACATGACT
    GTTGAGCAGC TCTGGACTGG ATCACCAACC TCTCCCACAG CAGAACCATC AGCTGCAACT
    GCTGCCACAG CTTCTGCTGA TATCAAACCC ACAAAGGAAA AACGCTTTCT GGATGACATT
    AAGAAACTTC AGGAGAATTT GAAAAAGACT CTAGACAATG TTGCCATTGT TGAAGAAGAT
    AAAATGGAGG CTCCCGTAGA AGAGGAAAGG ATTATCACTC CAGTGTTAAC TGAACCACAG
    ACACCTGTGC GTTCTGAATG GCCTAGTGAC ACTCCAGTTT TGTGCCAGCA GAGTGGAGGC
    AAACCAGGTG TCACCTTTAC CAGTGCTAAA GGGGAAGTGT TTTCTGTTTT GGAATGGGTA
    CCAGACACCC ACTCATTTAA AAAAATGGAA TTTCAGCCAG CAGAGGCAAA GAAGTTCTTT
    AGCACTGTAA GGAAAGAAAT GGCCTTGCTG GCCACCTCTC TGCCTGATGG TATCATGGTG
    AAAACCTTTG AGGACAGAAT GGACCTTTTT TCAGCTCTCA TTAAGGGCCC TCACCGCACT
    CCCTACGAGG ATGGACTCTT TCTCTTTGAT ATCCAGCTTC CTAATATCTA TCCTGCTGTT
    CCTCCTCTTT TCCGTTATTT GTCTCAGTGT AGCGGGCGGC TTAATCCTAA CCTTTATGAT
    AATGGAAAGG TTTGTGTCAG CCTTCTTGGC ACCTGGATTG GAAAGGGTAC TGAAAGATGG
    ACAAGCAAAT CAAGTTTACT TCAAGTGCTT ATCTCTATAC AAGGTCTGAT TCTGGTCAAT
    GAGCCATATT ACAATGAGGC TGGCTTTGAC AGTGACCGGG GACTTCAGGA AGGTTATGAA
    AATAGTCGCT GTTACAACGA GATGGCACTC ATTAAGCTGG TACAGTCAAT GACTCAGCTT
    CTTCAGCAGC CTGTAGAGGT CTTTCAGCAG GAAGTCTATG AACACTTTGC CTGCAGTGGT
    TGGCGGCTGG TCCATCGGCT GGAGTCCTGG CTAGAGTTAG ATGGGGCAAT CACAGATCAT
    GCTCCACGAT GTGGTGTTGG AAGCCTCTAT GCAGAACCTC TAGAAGAGAT CCCCTCAGTT
    CTTTACCACT GCAGTTCTAG CAAGCAGGAC ATACAAGTCG AAGAAGATTT TGAGGATTCG
    GGACTCAGTC TCTCTACAAT GGCAACCAGT GGACCTACTG GTTTAACTGA AAAAGATTTG
    AGCCAGAACT CTGAGTGTGA TAGTTCATTG GTGGGTGGTG CAGGATCAGA AATACCAGAA
    GTTCCTGAAG TAATGTCAAA TTTTTTGGGA TCTCAACAAA GTGTAAAATC ATGTCAGGAG
    TCTCAACCAG CTGTGAGACC AAAGAAAAGG AGAAAGAGTT ATCGTAGTTT CCTTCCAGAG
    CGGAGTGGTT ATCCTGATAT TGGATTCCCC CTCTTCCCTC TTTCCAAAGG TTTTGTAAAG
    AGCATGTGTG TAGTTCTGCA GCATTATCGA GCTACTCTCA CAGCTGCTGG TGTTCCTGAA
    CACCCAGAAA ACAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.