ASAP1A gene cDNA ORF clone, Danio rerio(Zebrafish)

mRNA	Protein	Name
XM_005163474.4	XP_005163531.1	arfGAP with SH3 domain, ankyrin repeat and PH domain 1a isoform X1
XM_005163478.4	XP_005163535.1	arfGAP with SH3 domain, ankyrin repeat and PH domain 1a isoform X4
NM_001130663.1	NP_001124135.1	ArfGAP with SH3 domain, ankyrin repeat and PH domain 1a
XM_005163475.4	XP_005163532.1	arfGAP with SH3 domain, ankyrin repeat and PH domain 1a isoform X2
XM_005163477.4	XP_005163534.1	arfGAP with SH3 domain, ankyrin repeat and PH domain 1a isoform X3
XM_017357489.1	XP_017212978.1	arfGAP with SH3 domain, ankyrin repeat and PH domain 1a isoform X1
XM_005163477.3	XP_005163534.1	arfGAP with SH3 domain, ankyrin repeat and PH domain 1a isoform X3
XM_005163474.3	XP_005163531.1	arfGAP with SH3 domain, ankyrin repeat and PH domain 1a isoform X1
XM_005163475.3	XP_005163532.1	arfGAP with SH3 domain, ankyrin repeat and PH domain 1a isoform X2
XM_005163478.3	XP_005163535.1	arfGAP with SH3 domain, ankyrin repeat and PH domain 1a isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa36266	NM_001130663.1 Latest version!	Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1a (asap1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa07129	XM_017357489.1 Latest version!	Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1a (asap1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa07129	XM_005163474.4 Latest version!	Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1a (asap1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa07130	XM_005163475.4 Latest version!	Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1a (asap1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa07132	XM_005163477.4 Latest version!	Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1a (asap1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa07133	XM_005163478.4 Latest version!	Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1a (asap1a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
ODa07132	XM_005163477.3	Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1a (asap1a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa07129	XM_005163474.3	Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1a (asap1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa07130	XM_005163475.3	Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1a (asap1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa07133	XM_005163478.3	Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1a (asap1a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

ASAP1A ( NM_001130663.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001124135.1 Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1a (asap1a), mRNA.

Price & Availability↑

CloneID	ODa36266
Clone ID Related Accession (Same CDS sequence)	NM_001130663.1
Accession Version	NM_001130663.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	ArfGAP with SH3 domain, ankyrin repeat and PH domain 1a
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC163042.1. ##Evidence-Data-START## Transcript exon combination :: BC163042.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCCGGATC AGATCTCCGT GTCCGAGTTT CTCTCGGAGA CGACGGAGGA TTACAATTCC 
CCCACGACCT CGAGCTTCAC CACCCGCCTG CAGAGCTGCC GGAACACGGT CAATGTTCTG 
GAAGAGGCTT TGGATCAGGA CCGAACTGCT TTACAGAAGG TCAAGAAATC TGTCAAAGCA 
ATCTACAACT CGGGTCAAGA ACATGTGCAG AATGAAGAGA ATTATGGACA GGCACTGGAC 
AAGTTTGGCA GCAACTTCAT CAGCCGAGAT AACTCTGATC TGGGAACAGC CTTCATCAAG 
TTTTCTGGAC TTATCAAAGA GCTGGCTGCT CTCCTCAAGA ACCTGCTCCA GAGCCTCAGC 
CACAACGTCA TCTTCACCCT GGACTCTCTG CTCAAAGGAG ATCTAAAGGG AGTGAAGGGG 
GACCTTAAAA AGCCTTTTGA CAAGGCCTGG AAAGACTATG AAACCAAGTT CACAAAGATC 
GAGAAGGAGA AGAGAGAACA TGCCAAGCAG CACGGCATGA TCCGCACAGA AATCACCGGC 
GCAGAGATTG CAGAAGAGAT GGAGAAGGAG CGGAGGATCT TTCAGCTGCA GATGTGTGAG 
TACCTGATCA AAGTCAATGA GATTAAGACC AAGAAGGGAG TGGATCTCCT CCAGAATCTC 
ATCAAGTATT ATCATGCACA GTGCAATTTC TTCCAGGATG GCTTGAAAAC TGCTGACAAG 
TTGAAGCAGT ATATTGAAAA ATTAGCAGCT GATCTTTATA ATATAAAACA GACTCAGGAT 
GAGGAGAAAA AACAGCTCAC AGCTCTCAGA GACCTCATCA AATCTTCCTT ACAGCTGGAC 
CAGAAGGAGG ATTCTCAGAG TAAGCAGAGC GGTTACAGCA TGCACCAGCT GCAGGGCAAT 
AAGGAGTTTG GCAGTGAGAA GAAGGGCTAT CTCTTTAAGA AGAGTGATGG GATCCGTAAG 
GTGTGGCAGA GGAGGAAGTG CTCAGTGAAA AATGGCATCC TCACCATCTC TCATGCCACA 
TCCAACAGGC AGCCGGTGAG ACTGAATCTG CTGACCTGCC AGGTTAAACC CAGTGGAGAG 
GATAAGAAGT GCTTTGACCT CATCTCTCAT AATCGAACAT ATCATTTCCA GGCAGAGGAC 
GAACAGGAGT TTGTGATATG GATCTCGGTG CTGACTAATA GTAAGGAGGA GGCTCTGAAC 
ATGGCATTTC GTGGGGAGCA GAGTGCTGGA GATGACAGTT TGGAGGACTT GACCAAAGCC 
ATCATCGAGG ACGTGCTGCG CATTCCTGGA AACGAAGTCT GCTGTGACTG TGGGGCTCCA 
GAGCCCAAAT GGTTATCCAC TAACCTCGGC ATCCTGACGT GCATCGAGTG TTCAGGAATC 
CACAGGGAAA TGGGAGTCCA TATTTCGCGC ATCCAATCCA TGGAGCTTGA CAAACTTGGA 
ACCTCTGAAC TCTTGCTGGC TAAGAACGTG GGCAACAGTA GTTTCAACGA AATATTAGAA 
GGGAATCTGC CGAGTCCTTC ACCAAAGCCA GCGCCATCAA GTGACATGAC CGAGAGGAAG 
GAGTACATCA ATGCGAAGTA CGTGGAGCAC AGGTTCGCTC GGCGAACGGC CACTACAGCC 
ACAGCCAGAC AGGGCGACTT GTACGAGGCG GTGAGAACGC GAGACTTGAT GGCTCTCATT 
CAGCTCTATG CAGATGGAGT GGAGCTAATG GATCCTTTCC CAGAAGCAGG ACAGGACCCG 
GGAGAGACAG CTCTGCACTT TGCTGTTCGG ACATCAGACC AGACTTCCCT GCACCTGGTG 
GACTTTCTTG TCCAAAACAG TGGGACTCTA GACAGACAGA CGGAGAGTGG AAACGCTGCT 
CTCCATTACT GCTGCACATA TGAGAAGCCA GAGTGTCTCA AACTGCTGCT CAGGGGAAAA 
CCGTCTATTG ACCTGGTTAA TCAAAACGGG GAGACAGCAT TGGATATCGC CAGACGACTG 
AGAAATGTAC AGTGTGAAGA GCTACTGGTG GAGGCAGCAG CCGGGAGGTT TAATCCTCAT 
GTGCATGTGG AGTATGAGTG GAATCTGCGG CTGGAGGAGA TTGATGAGAG TGACGATGAC 
CTGGATGACA AGCCCAGTCC AGTGAAGAAG GAGCGTTCTC CTCGTCCTCA GAGCTTCTGT 
CATTCGTCCA GCGTGTCTCC TCAGGAGAAG TTAACCCTGC CGGGGTATCT AGGACACAGG 
GACAAGCAGA GACTGTCCTA TGGAGCCTTT GCCAACCCCG TCTACAGCAC CTCCACCGAA 
ACCCCTGCAT CTCCAGTGTC AGAGGGACCC ACCATAGCCA GCAAGACCCC TGCAAAAGGA 
AGTGAGTCCA CACCTCCTTC TGCAAATCGG ACATCCCCGG CCAACAAGTT TGAGGGAATC 
CAGCAGCAGC AAAGCACTAC GTCTATGAAC ACAAAAGCAA CATTTGGCCC ACGAGTTCTT 
CCCAAACTAC CTCAAAAAGT GGCACTACGA AAGATTGACA CAATCCACCT CCCATCAGTG 
GACAAGTCTG GTCCTGATGT GCTTCAGAAA CCCCCACAGG CCCAGGATGC ACCTCCCACC 
AGAGCCTCAG ATACAATAAC CAGACCCACT GAACCTCCAC CTAAAATTCC ACAGGTCGCA 
GAACGATCCC AGCCTGTGGA TGTCCCGCAA AAACCGCACA TCTCAGACCT TCCTCCCAAA 
CCGCAACTAT CAGATCTTCC CCCCAAACCC CAATTGTCGG ATTTACCACC AAAACCTCAG 
CTTTCTGACC TGCCCCCGAA GCCTCAGCTT AAGGATCTTC CCCCTAAGCC GCAGATCAGT 
GATCTGCCAT CCAAACCGGC CGTGTGTTCT GCGTCTGAGG CCACACAGAG GCAGTCAACG 
CAGGAGGAAA CCAGTCCGAA GCCCCAGCTG ACGGAGACAC AGTCATTCAG CCAGCAGGAG 
GAGCTCTCAC CCCGACAGGC CAGCGAGGAC ACCAATGGAG CGCCCGCAGG AGCCTTGGAA 
ATGCCAGTCC CAATGCCACG CAAAATTAAC ACAGTAGCAA AGAATAAAGC GAAACGTGTG 
AAAACTATCT ATGATTGCCA GGCAGACAAT GACGATGAGC TGACTTTTGT GGAGGGCGAG 
GTTATAATTG TCACAGGAGA GGAAGACCAG GAGTGGTGGA TCGGGCACAT AGAGGGTCAG 
CCTGAAAGGA AAGGGGTCTT CCCAATGTCC TTCGTGCACA TTCTGTCAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124135.1
CDS	100..3333
Translation	MPDQISVSEFLSETTEDYNSPTTSSFTTRLQSCRNTVNVLEEALDQDRTALQKVKKSVKAIYNSGQEHVQNEENYGQALDKFGSNFISRDNSDLGTAFIKFSGLIKELAALLKNLLQSLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRIFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKEDSQSKQSGYSMHQLQGNKEFGSEKKGYLFKKSDGIRKVWQRRKCSVKNGILTISHATSNRQPVRLNLLTCQVKPSGEDKKCFDLISHNRTYHFQAEDEQEFVIWISVLTNSKEEALNMAFRGEQSAGDDSLEDLTKAIIEDVLRIPGNEVCCDCGAPEPKWLSTNLGILTCIECSGIHREMGVHISRIQSMELDKLGTSELLLAKNVGNSSFNEILEGNLPSPSPKPAPSSDMTERKEYINAKYVEHRFARRTATTATARQGDLYEAVRTRDLMALIQLYADGVELMDPFPEAGQDPGETALHFAVRTSDQTSLHLVDFLVQNSGTLDRQTESGNAALHYCCTYEKPECLKLLLRGKPSIDLVNQNGETALDIARRLRNVQCEELLVEAAAGRFNPHVHVEYEWNLRLEEIDESDDDLDDKPSPVKKERSPRPQSFCHSSSVSPQEKLTLPGYLGHRDKQRLSYGAFANPVYSTSTETPASPVSEGPTIASKTPAKGSESTPPSANRTSPANKFEGIQQQQSTTSMNTKATFGPRVLPKLPQKVALRKIDTIHLPSVDKSGPDVLQKPPQAQDAPPTRASDTITRPTEPPPKIPQVAERSQPVDVPQKPHISDLPPKPQLSDLPPKPQLSDLPPKPQLSDLPPKPQLKDLPPKPQISDLPSKPAVCSASEATQRQSTQEETSPKPQLTETQSFSQQEELSPRQASEDTNGAPAGALEMPVPMPRKINTVAKNKAKRVKTIYDCQADNDDELTFVEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPMSFVHILSD

Target ORF information:

RefSeq Version	NM_001130663.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1a (asap1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130663.1

ORF Insert Sequence:

ATGCCGGATC AGATCTCCGT GTCCGAGTTT CTCTCGGAGA CGACGGAGGA TTACAATTCC 
CCCACGACCT CGAGCTTCAC CACCCGCCTG CAGAGCTGCC GGAACACGGT CAATGTTCTG 
GAAGAGGCTT TGGATCAGGA CCGAACTGCT TTACAGAAGG TCAAGAAATC TGTCAAAGCA 
ATCTACAACT CGGGTCAAGA ACATGTGCAG AATGAAGAGA ATTATGGACA GGCACTGGAC 
AAGTTTGGCA GCAACTTCAT CAGCCGAGAT AACTCTGATC TGGGAACAGC CTTCATCAAG 
TTTTCTGGAC TTATCAAAGA GCTGGCTGCT CTCCTCAAGA ACCTGCTCCA GAGCCTCAGC 
CACAACGTCA TCTTCACCCT GGACTCTCTG CTCAAAGGAG ATCTAAAGGG AGTGAAGGGG 
GACCTTAAAA AGCCTTTTGA CAAGGCCTGG AAAGACTATG AAACCAAGTT CACAAAGATC 
GAGAAGGAGA AGAGAGAACA TGCCAAGCAG CACGGCATGA TCCGCACAGA AATCACCGGC 
GCAGAGATTG CAGAAGAGAT GGAGAAGGAG CGGAGGATCT TTCAGCTGCA GATGTGTGAG 
TACCTGATCA AAGTCAATGA GATTAAGACC AAGAAGGGAG TGGATCTCCT CCAGAATCTC 
ATCAAGTATT ATCATGCACA GTGCAATTTC TTCCAGGATG GCTTGAAAAC TGCTGACAAG 
TTGAAGCAGT ATATTGAAAA ATTAGCAGCT GATCTTTATA ATATAAAACA GACTCAGGAT 
GAGGAGAAAA AACAGCTCAC AGCTCTCAGA GACCTCATCA AATCTTCCTT ACAGCTGGAC 
CAGAAGGAGG ATTCTCAGAG TAAGCAGAGC GGTTACAGCA TGCACCAGCT GCAGGGCAAT 
AAGGAGTTTG GCAGTGAGAA GAAGGGCTAT CTCTTTAAGA AGAGTGATGG GATCCGTAAG 
GTGTGGCAGA GGAGGAAGTG CTCAGTGAAA AATGGCATCC TCACCATCTC TCATGCCACA 
TCCAACAGGC AGCCGGTGAG ACTGAATCTG CTGACCTGCC AGGTTAAACC CAGTGGAGAG 
GATAAGAAGT GCTTTGACCT CATCTCTCAT AATCGAACAT ATCATTTCCA GGCAGAGGAC 
GAACAGGAGT TTGTGATATG GATCTCGGTG CTGACTAATA GTAAGGAGGA GGCTCTGAAC 
ATGGCATTTC GTGGGGAGCA GAGTGCTGGA GATGACAGTT TGGAGGACTT GACCAAAGCC 
ATCATCGAGG ACGTGCTGCG CATTCCTGGA AACGAAGTCT GCTGTGACTG TGGGGCTCCA 
GAGCCCAAAT GGTTATCCAC TAACCTCGGC ATCCTGACGT GCATCGAGTG TTCAGGAATC 
CACAGGGAAA TGGGAGTCCA TATTTCGCGC ATCCAATCCA TGGAGCTTGA CAAACTTGGA 
ACCTCTGAAC TCTTGCTGGC TAAGAACGTG GGCAACAGTA GTTTCAACGA AATATTAGAA 
GGGAATCTGC CGAGTCCTTC ACCAAAGCCA GCGCCATCAA GTGACATGAC CGAGAGGAAG 
GAGTACATCA ATGCGAAGTA CGTGGAGCAC AGGTTCGCTC GGCGAACGGC CACTACAGCC 
ACAGCCAGAC AGGGCGACTT GTACGAGGCG GTGAGAACGC GAGACTTGAT GGCTCTCATT 
CAGCTCTATG CAGATGGAGT GGAGCTAATG GATCCTTTCC CAGAAGCAGG ACAGGACCCG 
GGAGAGACAG CTCTGCACTT TGCTGTTCGG ACATCAGACC AGACTTCCCT GCACCTGGTG 
GACTTTCTTG TCCAAAACAG TGGGACTCTA GACAGACAGA CGGAGAGTGG AAACGCTGCT 
CTCCATTACT GCTGCACATA TGAGAAGCCA GAGTGTCTCA AACTGCTGCT CAGGGGAAAA 
CCGTCTATTG ACCTGGTTAA TCAAAACGGG GAGACAGCAT TGGATATCGC CAGACGACTG 
AGAAATGTAC AGTGTGAAGA GCTACTGGTG GAGGCAGCAG CCGGGAGGTT TAATCCTCAT 
GTGCATGTGG AGTATGAGTG GAATCTGCGG CTGGAGGAGA TTGATGAGAG TGACGATGAC 
CTGGATGACA AGCCCAGTCC AGTGAAGAAG GAGCGTTCTC CTCGTCCTCA GAGCTTCTGT 
CATTCGTCCA GCGTGTCTCC TCAGGAGAAG TTAACCCTGC CGGGGTATCT AGGACACAGG 
GACAAGCAGA GACTGTCCTA TGGAGCCTTT GCCAACCCCG TCTACAGCAC CTCCACCGAA 
ACCCCTGCAT CTCCAGTGTC AGAGGGACCC ACCATAGCCA GCAAGACCCC TGCAAAAGGA 
AGTGAGTCCA CACCTCCTTC TGCAAATCGG ACATCCCCGG CCAACAAGTT TGAGGGAATC 
CAGCAGCAGC AAAGCACTAC GTCTATGAAC ACAAAAGCAA CATTTGGCCC ACGAGTTCTT 
CCCAAACTAC CTCAAAAAGT GGCACTACGA AAGATTGACA CAATCCACCT CCCATCAGTG 
GACAAGTCTG GTCCTGATGT GCTTCAGAAA CCCCCACAGG CCCAGGATGC ACCTCCCACC 
AGAGCCTCAG ATACAATAAC CAGACCCACT GAACCTCCAC CTAAAATTCC ACAGGTCGCA 
GAACGATCCC AGCCTGTGGA TGTCCCGCAA AAACCGCACA TCTCAGACCT TCCTCCCAAA 
CCGCAACTAT CAGATCTTCC CCCCAAACCC CAATTGTCGG ATTTACCACC AAAACCTCAG 
CTTTCTGACC TGCCCCCGAA GCCTCAGCTT AAGGATCTTC CCCCTAAGCC GCAGATCAGT 
GATCTGCCAT CCAAACCGGC CGTGTGTTCT GCGTCTGAGG CCACACAGAG GCAGTCAACG 
CAGGAGGAAA CCAGTCCGAA GCCCCAGCTG ACGGAGACAC AGTCATTCAG CCAGCAGGAG 
GAGCTCTCAC CCCGACAGGC CAGCGAGGAC ACCAATGGAG CGCCCGCAGG AGCCTTGGAA 
ATGCCAGTCC CAATGCCACG CAAAATTAAC ACAGTAGCAA AGAATAAAGC GAAACGTGTG 
AAAACTATCT ATGATTGCCA GGCAGACAAT GACGATGAGC TGACTTTTGT GGAGGGCGAG 
GTTATAATTG TCACAGGAGA GGAAGACCAG GAGTGGTGGA TCGGGCACAT AGAGGGTCAG 
CCTGAAAGGA AAGGGGTCTT CCCAATGTCC TTCGTGCACA TTCTGTCAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASAP1A ( XM_005163474.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005163531.1 Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1a (asap1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa07129
Clone ID Related Accession (Same CDS sequence)	XM_005163474.4 , XM_017357489.1 , XM_005163474.3
Accession Version	XM_005163474.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	arfGAP with SH3 domain, ankyrin repeat and PH domain 1a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005163474.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTCCT CGTCCTCGCG TTTGTCAAGT TTTTCCTCCA GGGATTCATT ATGGAGTCGG 
ATGCCGGATC AGATCTCCGT GTCCGAGTTT CTCTCGGAGA CGACGGAGGA TTACAATTCC 
CCTACGACCT CGAGCTTCAC CACCCGCCTG CAGAGCTGCC GGAACACGGT CAATGTTCTG 
GAAGAGGCTT TGGATCAGGA CCGAACTGCT TTACAGAAGG TCAAGAAATC TGTCAAAGCA 
ATCTACAACT CGGGTCAAGA ACATGTGCAG AATGAAGAGA ATTATGGACA GGCACTGGAC 
AAGTTTGGCA GCAACTTCAT CAGCCGAGAT AACTCTGATC TGGGAACAGC CTTCATCAAG 
TTTTCTGGAC TTATCAAAGA GCTGGCTGCT CTCCTCAAGA ACCTGCTCCA GAGCCTCAGC 
CACAACGTCA TCTTCACCCT GGACTCTCTG CTCAAAGGAG ATCTAAAGGG AGTGAAGGGG 
GACCTTAAAA AGCCTTTTGA CAAGGCCTGG AAAGACTATG AAACCAAGTT CACAAAGATC 
GAGAAGGAGA AGAGAGAACA TGCCAAGCAG CACGGCATGA TCCGCACAGA GATCACCGGC 
GCAGAGATTG CAGAAGAGAT GGAGAAGGAG CGGAGGATCT TTCAGCTGCA GATGTGTGAG 
TACCTGATCA AAGTCAATGA GATTAAGACC AAGAAGGGAG TGGATCTCCT CCAGAATCTC 
ATCAAGTATT ATCATGCACA GTGCAATTTC TTCCAGGATG GCTTGAAAAC TGCTGACAAG 
TTGAAGCAGT ATATTGAAAA ATTAGCAGCT GATCTTTATA ATATAAAACA GACTCAGGAT 
GAGGAGAAAA AACAGCTCAC AGCTCTCAGA GACCTCATCA AATCTTCCTT ACAGCTGGAC 
CAGAAGGAGT CTAGGAGAGA TTCTCAGAGT AAGCAGAGCG GTTACAGCAT GCACCAGCTG 
CAGGGCAATA AGGAGTTTGG CAGTGAGAAG AAGGGCTATC TCTTTAAGAA GAGTGATGGG 
ATCCGTAAGG TGTGGCAGAG GAGGAAGTGC TCAGTGAAAA ATGGCATCCT CACCATCTCT 
CATGCCACAT CCAACAGGCA GCCGGTGAGA CTGAATCTGC TGACCTGCCA GGTTAAACCC 
AGTGGAGAGG ATAAGAAGTG CTTTGACCTC ATCTCTCATA ATCGAACATA TCATTTCCAG 
GCAGAGGACG AACAGGAGTT TGTGATATGG ATCTCGGTGC TGACTAATAG TAAGGAGGAG 
GCTCTGAACA TGGCATTTCG TGGGGAGCAG AGTGCTGGAG ATGACAGTTT GGAGGACTTG 
ACCAAAGCCA TCATCGAGGA CGTGCTGCGC ATTCCTGGAA ACGAAGTCTG CTGTGACTGT 
GGGGCTCCAG AGCCCAAATG GTTATCCACT AACCTCGGCA TCCTGACGTG CATCGAGTGT 
TCAGGAATCC ACAGGGAAAT GGGAGTCCAT ATTTCGCGCA TCCAATCCAT GGAGCTTGAC 
AAACTTGGAA CCTCTGAACT CTTGCTGGCT AAGAACGTGG GCAACAGTAG TTTCAACGAA 
ATATTAGAAG GGAATCTGCC GAGTCCTTCA CCAAAGCCAG CGCCATCAAG TGACATGACC 
GAGAGGAAGG AGTACATCAA TGCGAAGTAC GTGGAGCACA GGTTCGCTCG GCGAACGGCC 
ACTACAGCCA CAGCCAGACA GGGCGACTTG TACGAGGCGG TGAGAACGCG AGACTTGATG 
GCTCTCATTC AGCTCTATGC AGATGGAGTG GAGCTAATGG ATCCTTTCCC AGAAGCAGGA 
CAGGACCCGG GAGAGACAGC TCTGCACTTT GCTGTTCGGA CATCAGACCA GACTTCCCTG 
CACCTGGTGG ACTTTCTTGT CCAAAACAGT GGGACTCTAG ACAGACAGAC GGAGAGTGGA 
AACGCTGCTC TCCATTACTG CTGCACATAT GAGAAGCCAG AGTGTCTCAA ACTGCTGCTC 
AGGGGAAAAC CGTCTATTGA CCTGGTTAAT CAAAACGGGG AGACAGCATT GGATATCGCC 
AGACGACTGA GAAATGTACA GTGTGAAGAG CTACTGGTGG AGGCAGCAGC CGGGAGGTTT 
AATCCTCATG TGCATGTGGA GTATGAGTGG AATCTGCGGC TGGAGGAGAT TGATGAGAGT 
GACGATGACC TGGATGACAA GCCCAGTCCA GTGAAGAAGG AGCGTTCTCC TCGTCCTCAG 
AGCTTCTGTC ATTCGTCCAG CGTGTCTCCT CAGGAGAAGT TAACCCTGCC GGGGTATCTA 
GGACACAGGG ACAAGCAGAG ACTGTCCTAT GGAGCCTTTG CCAACCCCGT CTACAGCACC 
TCCACCGAAA CCCCTGCATC TCCAGTGTCA GAGGGACCCA CCATAGCCAG CAAGACCCCT 
GCAAAAGCTC CGTCCTGTGG GCCGCCCACC TCTCTGCCGC TGGGATCTCA ATCGAGTGCA 
GGAGGCAGCT CCACTCTGTC TAAGAAGAGA GCTCCTCCTC CACCTCCCGG ACACAAGCGC 
ACCCACTCAG ATCCCCCCAG TCCCGTACTG CAGGGTCCGC AGAGCAAAGG AAGTGAGTCC 
ACACCTCCTT CTGCAAATCG GACATCCCCG GCCAACAAGT TTGAGGGAAT CCAGCAGCAG 
CAAAGCACTA CGTCTATGAA CACAAAAGCA ACATTTGGCC CACGAGTTCT TCCCAAACTA 
CCTCAAAAAG TGGCACTACG AAAGATTGAC ACAATCCACC TCCCATCAGT GGACAAGTCT 
GGTCCTGATG TGCTTCAGAA ACCCCCACAG GCCCAGGATG CACCTCCCAC CAGAGCCTCA 
GATACAATAA CCAGACCCAC TGAACCTCCA CCTAAAATTC CACAGGTCGC AGAACGATCC 
CAGCCTGTGG ATGTCCCGCA GAAACCGCAC ATCTCAGACC TTCCTCCCAA ACCGCAACTA 
TCAGATCTTC CCCCCAAACC CCAATTGTCG GATTTACCAC CAAAACCTCA GCTTTCTGAC 
CTGCCCCCGA AGCCTCAGCT TAAGGATCTT CCCCCTAAGC CGCAGATCAG TGATCTGCCA 
TCCAAACCGG CCGTGTGTTC TGCATCTGAG GCCACACAAA GGCAGTCAAC GCAGGAGGAA 
ACCAGTCCGA AGCCCCAGCT GACGGAGACA CAGTCATTCA GCCAGCAGGA GGAGCTCTCA 
CCCCGACAGG CCAGCGAGGA CACCAATGGA GCGCCCGCAG GAGCCTTGGA AATGCCAGTC 
CCAATGCCAC GCAAAATTAA CACAGTAGCA AAGAACAAAG CGAAGCGTGT GAAAACCATC 
TATGATTGCC AGGCAGACAA TGACGATGAG CTGACTTTTG TGGAGGGCGA GGTTATAATT 
GTCACAGGAG AGGAAGACCA GGAGTGGTGG ATCGGGCACA TAGAGGGTCA GCCTGAAAGG 
AAAGGGGTCT TCCCAATGTC CTTCGTGCAC ATTCTGTCAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163531.1
CDS	275..3739
Translation	MRSSSSRLSSFSSRDSLWSRMPDQISVSEFLSETTEDYNSPTTSSFTTRLQSCRNTVNVLEEALDQDRTALQKVKKSVKAIYNSGQEHVQNEENYGQALDKFGSNFISRDNSDLGTAFIKFSGLIKELAALLKNLLQSLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRIFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKESRRDSQSKQSGYSMHQLQGNKEFGSEKKGYLFKKSDGIRKVWQRRKCSVKNGILTISHATSNRQPVRLNLLTCQVKPSGEDKKCFDLISHNRTYHFQAEDEQEFVIWISVLTNSKEEALNMAFRGEQSAGDDSLEDLTKAIIEDVLRIPGNEVCCDCGAPEPKWLSTNLGILTCIECSGIHREMGVHISRIQSMELDKLGTSELLLAKNVGNSSFNEILEGNLPSPSPKPAPSSDMTERKEYINAKYVEHRFARRTATTATARQGDLYEAVRTRDLMALIQLYADGVELMDPFPEAGQDPGETALHFAVRTSDQTSLHLVDFLVQNSGTLDRQTESGNAALHYCCTYEKPECLKLLLRGKPSIDLVNQNGETALDIARRLRNVQCEELLVEAAAGRFNPHVHVEYEWNLRLEEIDESDDDLDDKPSPVKKERSPRPQSFCHSSSVSPQEKLTLPGYLGHRDKQRLSYGAFANPVYSTSTETPASPVSEGPTIASKTPAKAPSCGPPTSLPLGSQSSAGGSSTLSKKRAPPPPPGHKRTHSDPPSPVLQGPQSKGSESTPPSANRTSPANKFEGIQQQQSTTSMNTKATFGPRVLPKLPQKVALRKIDTIHLPSVDKSGPDVLQKPPQAQDAPPTRASDTITRPTEPPPKIPQVAERSQPVDVPQKPHISDLPPKPQLSDLPPKPQLSDLPPKPQLSDLPPKPQLKDLPPKPQISDLPSKPAVCSASEATQRQSTQEETSPKPQLTETQSFSQQEELSPRQASEDTNGAPAGALEMPVPMPRKINTVAKNKAKRVKTIYDCQADNDDELTFVEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPMSFVHILSD

Target ORF information:

RefSeq Version	XM_005163474.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1a (asap1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163474.4

ORF Insert Sequence:

ATGAGGTCCT CGTCCTCGCG TTTGTCAAGT TTTTCCTCCA GGGATTCATT ATGGAGTCGG 
ATGCCGGATC AGATCTCCGT GTCCGAGTTT CTCTCGGAGA CGACGGAGGA TTACAATTCC 
CCTACGACCT CGAGCTTCAC CACCCGCCTG CAGAGCTGCC GGAACACGGT CAATGTTCTG 
GAAGAGGCTT TGGATCAGGA CCGAACTGCT TTACAGAAGG TCAAGAAATC TGTCAAAGCA 
ATCTACAACT CGGGTCAAGA ACATGTGCAG AATGAAGAGA ATTATGGACA GGCACTGGAC 
AAGTTTGGCA GCAACTTCAT CAGCCGAGAT AACTCTGATC TGGGAACAGC CTTCATCAAG 
TTTTCTGGAC TTATCAAAGA GCTGGCTGCT CTCCTCAAGA ACCTGCTCCA GAGCCTCAGC 
CACAACGTCA TCTTCACCCT GGACTCTCTG CTCAAAGGAG ATCTAAAGGG AGTGAAGGGG 
GACCTTAAAA AGCCTTTTGA CAAGGCCTGG AAAGACTATG AAACCAAGTT CACAAAGATC 
GAGAAGGAGA AGAGAGAACA TGCCAAGCAG CACGGCATGA TCCGCACAGA GATCACCGGC 
GCAGAGATTG CAGAAGAGAT GGAGAAGGAG CGGAGGATCT TTCAGCTGCA GATGTGTGAG 
TACCTGATCA AAGTCAATGA GATTAAGACC AAGAAGGGAG TGGATCTCCT CCAGAATCTC 
ATCAAGTATT ATCATGCACA GTGCAATTTC TTCCAGGATG GCTTGAAAAC TGCTGACAAG 
TTGAAGCAGT ATATTGAAAA ATTAGCAGCT GATCTTTATA ATATAAAACA GACTCAGGAT 
GAGGAGAAAA AACAGCTCAC AGCTCTCAGA GACCTCATCA AATCTTCCTT ACAGCTGGAC 
CAGAAGGAGT CTAGGAGAGA TTCTCAGAGT AAGCAGAGCG GTTACAGCAT GCACCAGCTG 
CAGGGCAATA AGGAGTTTGG CAGTGAGAAG AAGGGCTATC TCTTTAAGAA GAGTGATGGG 
ATCCGTAAGG TGTGGCAGAG GAGGAAGTGC TCAGTGAAAA ATGGCATCCT CACCATCTCT 
CATGCCACAT CCAACAGGCA GCCGGTGAGA CTGAATCTGC TGACCTGCCA GGTTAAACCC 
AGTGGAGAGG ATAAGAAGTG CTTTGACCTC ATCTCTCATA ATCGAACATA TCATTTCCAG 
GCAGAGGACG AACAGGAGTT TGTGATATGG ATCTCGGTGC TGACTAATAG TAAGGAGGAG 
GCTCTGAACA TGGCATTTCG TGGGGAGCAG AGTGCTGGAG ATGACAGTTT GGAGGACTTG 
ACCAAAGCCA TCATCGAGGA CGTGCTGCGC ATTCCTGGAA ACGAAGTCTG CTGTGACTGT 
GGGGCTCCAG AGCCCAAATG GTTATCCACT AACCTCGGCA TCCTGACGTG CATCGAGTGT 
TCAGGAATCC ACAGGGAAAT GGGAGTCCAT ATTTCGCGCA TCCAATCCAT GGAGCTTGAC 
AAACTTGGAA CCTCTGAACT CTTGCTGGCT AAGAACGTGG GCAACAGTAG TTTCAACGAA 
ATATTAGAAG GGAATCTGCC GAGTCCTTCA CCAAAGCCAG CGCCATCAAG TGACATGACC 
GAGAGGAAGG AGTACATCAA TGCGAAGTAC GTGGAGCACA GGTTCGCTCG GCGAACGGCC 
ACTACAGCCA CAGCCAGACA GGGCGACTTG TACGAGGCGG TGAGAACGCG AGACTTGATG 
GCTCTCATTC AGCTCTATGC AGATGGAGTG GAGCTAATGG ATCCTTTCCC AGAAGCAGGA 
CAGGACCCGG GAGAGACAGC TCTGCACTTT GCTGTTCGGA CATCAGACCA GACTTCCCTG 
CACCTGGTGG ACTTTCTTGT CCAAAACAGT GGGACTCTAG ACAGACAGAC GGAGAGTGGA 
AACGCTGCTC TCCATTACTG CTGCACATAT GAGAAGCCAG AGTGTCTCAA ACTGCTGCTC 
AGGGGAAAAC CGTCTATTGA CCTGGTTAAT CAAAACGGGG AGACAGCATT GGATATCGCC 
AGACGACTGA GAAATGTACA GTGTGAAGAG CTACTGGTGG AGGCAGCAGC CGGGAGGTTT 
AATCCTCATG TGCATGTGGA GTATGAGTGG AATCTGCGGC TGGAGGAGAT TGATGAGAGT 
GACGATGACC TGGATGACAA GCCCAGTCCA GTGAAGAAGG AGCGTTCTCC TCGTCCTCAG 
AGCTTCTGTC ATTCGTCCAG CGTGTCTCCT CAGGAGAAGT TAACCCTGCC GGGGTATCTA 
GGACACAGGG ACAAGCAGAG ACTGTCCTAT GGAGCCTTTG CCAACCCCGT CTACAGCACC 
TCCACCGAAA CCCCTGCATC TCCAGTGTCA GAGGGACCCA CCATAGCCAG CAAGACCCCT 
GCAAAAGCTC CGTCCTGTGG GCCGCCCACC TCTCTGCCGC TGGGATCTCA ATCGAGTGCA 
GGAGGCAGCT CCACTCTGTC TAAGAAGAGA GCTCCTCCTC CACCTCCCGG ACACAAGCGC 
ACCCACTCAG ATCCCCCCAG TCCCGTACTG CAGGGTCCGC AGAGCAAAGG AAGTGAGTCC 
ACACCTCCTT CTGCAAATCG GACATCCCCG GCCAACAAGT TTGAGGGAAT CCAGCAGCAG 
CAAAGCACTA CGTCTATGAA CACAAAAGCA ACATTTGGCC CACGAGTTCT TCCCAAACTA 
CCTCAAAAAG TGGCACTACG AAAGATTGAC ACAATCCACC TCCCATCAGT GGACAAGTCT 
GGTCCTGATG TGCTTCAGAA ACCCCCACAG GCCCAGGATG CACCTCCCAC CAGAGCCTCA 
GATACAATAA CCAGACCCAC TGAACCTCCA CCTAAAATTC CACAGGTCGC AGAACGATCC 
CAGCCTGTGG ATGTCCCGCA GAAACCGCAC ATCTCAGACC TTCCTCCCAA ACCGCAACTA 
TCAGATCTTC CCCCCAAACC CCAATTGTCG GATTTACCAC CAAAACCTCA GCTTTCTGAC 
CTGCCCCCGA AGCCTCAGCT TAAGGATCTT CCCCCTAAGC CGCAGATCAG TGATCTGCCA 
TCCAAACCGG CCGTGTGTTC TGCATCTGAG GCCACACAAA GGCAGTCAAC GCAGGAGGAA 
ACCAGTCCGA AGCCCCAGCT GACGGAGACA CAGTCATTCA GCCAGCAGGA GGAGCTCTCA 
CCCCGACAGG CCAGCGAGGA CACCAATGGA GCGCCCGCAG GAGCCTTGGA AATGCCAGTC 
CCAATGCCAC GCAAAATTAA CACAGTAGCA AAGAACAAAG CGAAGCGTGT GAAAACCATC 
TATGATTGCC AGGCAGACAA TGACGATGAG CTGACTTTTG TGGAGGGCGA GGTTATAATT 
GTCACAGGAG AGGAAGACCA GGAGTGGTGG ATCGGGCACA TAGAGGGTCA GCCTGAAAGG 
AAAGGGGTCT TCCCAATGTC CTTCGTGCAC ATTCTGTCAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASAP1A ( XM_017357489.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017212978.1 Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1a (asap1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa07129
Clone ID Related Accession (Same CDS sequence)	XM_005163474.4 , XM_017357489.1 , XM_005163474.3
Accession Version	XM_017357489.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	arfGAP with SH3 domain, ankyrin repeat and PH domain 1a isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTCCT CGTCCTCGCG TTTGTCAAGT TTTTCCTCCA GGGATTCATT ATGGAGTCGG 
ATGCCGGATC AGATCTCCGT GTCCGAGTTT CTCTCGGAGA CGACGGAGGA TTACAATTCC 
CCTACGACCT CGAGCTTCAC CACCCGCCTG CAGAGCTGCC GGAACACGGT CAATGTTCTG 
GAAGAGGCTT TGGATCAGGA CCGAACTGCT TTACAGAAGG TCAAGAAATC TGTCAAAGCA 
ATCTACAACT CGGGTCAAGA ACATGTGCAG AATGAAGAGA ATTATGGACA GGCACTGGAC 
AAGTTTGGCA GCAACTTCAT CAGCCGAGAT AACTCTGATC TGGGAACAGC CTTCATCAAG 
TTTTCTGGAC TTATCAAAGA GCTGGCTGCT CTCCTCAAGA ACCTGCTCCA GAGCCTCAGC 
CACAACGTCA TCTTCACCCT GGACTCTCTG CTCAAAGGAG ATCTAAAGGG AGTGAAGGGG 
GACCTTAAAA AGCCTTTTGA CAAGGCCTGG AAAGACTATG AAACCAAGTT CACAAAGATC 
GAGAAGGAGA AGAGAGAACA TGCCAAGCAG CACGGCATGA TCCGCACAGA GATCACCGGC 
GCAGAGATTG CAGAAGAGAT GGAGAAGGAG CGGAGGATCT TTCAGCTGCA GATGTGTGAG 
TACCTGATCA AAGTCAATGA GATTAAGACC AAGAAGGGAG TGGATCTCCT CCAGAATCTC 
ATCAAGTATT ATCATGCACA GTGCAATTTC TTCCAGGATG GCTTGAAAAC TGCTGACAAG 
TTGAAGCAGT ATATTGAAAA ATTAGCAGCT GATCTTTATA ATATAAAACA GACTCAGGAT 
GAGGAGAAAA AACAGCTCAC AGCTCTCAGA GACCTCATCA AATCTTCCTT ACAGCTGGAC 
CAGAAGGAGT CTAGGAGAGA TTCTCAGAGT AAGCAGAGCG GTTACAGCAT GCACCAGCTG 
CAGGGCAATA AGGAGTTTGG CAGTGAGAAG AAGGGCTATC TCTTTAAGAA GAGTGATGGG 
ATCCGTAAGG TGTGGCAGAG GAGGAAGTGC TCAGTGAAAA ATGGCATCCT CACCATCTCT 
CATGCCACAT CCAACAGGCA GCCGGTGAGA CTGAATCTGC TGACCTGCCA GGTTAAACCC 
AGTGGAGAGG ATAAGAAGTG CTTTGACCTC ATCTCTCATA ATCGAACATA TCATTTCCAG 
GCAGAGGACG AACAGGAGTT TGTGATATGG ATCTCGGTGC TGACTAATAG TAAGGAGGAG 
GCTCTGAACA TGGCATTTCG TGGGGAGCAG AGTGCTGGAG ATGACAGTTT GGAGGACTTG 
ACCAAAGCCA TCATCGAGGA CGTGCTGCGC ATTCCTGGAA ACGAAGTCTG CTGTGACTGT 
GGGGCTCCAG AGCCCAAATG GTTATCCACT AACCTCGGCA TCCTGACGTG CATCGAGTGT 
TCAGGAATCC ACAGGGAAAT GGGAGTCCAT ATTTCGCGCA TCCAATCCAT GGAGCTTGAC 
AAACTTGGAA CCTCTGAACT CTTGCTGGCT AAGAACGTGG GCAACAGTAG TTTCAACGAA 
ATATTAGAAG GGAATCTGCC GAGTCCTTCA CCAAAGCCAG CGCCATCAAG TGACATGACC 
GAGAGGAAGG AGTACATCAA TGCGAAGTAC GTGGAGCACA GGTTCGCTCG GCGAACGGCC 
ACTACAGCCA CAGCCAGACA GGGCGACTTG TACGAGGCGG TGAGAACGCG AGACTTGATG 
GCTCTCATTC AGCTCTATGC AGATGGAGTG GAGCTAATGG ATCCTTTCCC AGAAGCAGGA 
CAGGACCCGG GAGAGACAGC TCTGCACTTT GCTGTTCGGA CATCAGACCA GACTTCCCTG 
CACCTGGTGG ACTTTCTTGT CCAAAACAGT GGGACTCTAG ACAGACAGAC GGAGAGTGGA 
AACGCTGCTC TCCATTACTG CTGCACATAT GAGAAGCCAG AGTGTCTCAA ACTGCTGCTC 
AGGGGAAAAC CGTCTATTGA CCTGGTTAAT CAAAACGGGG AGACAGCATT GGATATCGCC 
AGACGACTGA GAAATGTACA GTGTGAAGAG CTACTGGTGG AGGCAGCAGC CGGGAGGTTT 
AATCCTCATG TGCATGTGGA GTATGAGTGG AATCTGCGGC TGGAGGAGAT TGATGAGAGT 
GACGATGACC TGGATGACAA GCCCAGTCCA GTGAAGAAGG AGCGTTCTCC TCGTCCTCAG 
AGCTTCTGTC ATTCGTCCAG CGTGTCTCCT CAGGAGAAGT TAACCCTGCC GGGGTATCTA 
GGACACAGGG ACAAGCAGAG ACTGTCCTAT GGAGCCTTTG CCAACCCCGT CTACAGCACC 
TCCACCGAAA CCCCTGCATC TCCAGTGTCA GAGGGACCCA CCATAGCCAG CAAGACCCCT 
GCAAAAGCTC CGTCCTGTGG GCCGCCCACC TCTCTGCCGC TGGGATCTCA ATCGAGTGCA 
GGAGGCAGCT CCACTCTGTC TAAGAAGAGA GCTCCTCCTC CACCTCCCGG ACACAAGCGC 
ACCCACTCAG ATCCCCCCAG TCCCGTACTG CAGGGTCCGC AGAGCAAAGG AAGTGAGTCC 
ACACCTCCTT CTGCAAATCG GACATCCCCG GCCAACAAGT TTGAGGGAAT CCAGCAGCAG 
CAAAGCACTA CGTCTATGAA CACAAAAGCA ACATTTGGCC CACGAGTTCT TCCCAAACTA 
CCTCAAAAAG TGGCACTACG AAAGATTGAC ACAATCCACC TCCCATCAGT GGACAAGTCT 
GGTCCTGATG TGCTTCAGAA ACCCCCACAG GCCCAGGATG CACCTCCCAC CAGAGCCTCA 
GATACAATAA CCAGACCCAC TGAACCTCCA CCTAAAATTC CACAGGTCGC AGAACGATCC 
CAGCCTGTGG ATGTCCCGCA GAAACCGCAC ATCTCAGACC TTCCTCCCAA ACCGCAACTA 
TCAGATCTTC CCCCCAAACC CCAATTGTCG GATTTACCAC CAAAACCTCA GCTTTCTGAC 
CTGCCCCCGA AGCCTCAGCT TAAGGATCTT CCCCCTAAGC CGCAGATCAG TGATCTGCCA 
TCCAAACCGG CCGTGTGTTC TGCATCTGAG GCCACACAAA GGCAGTCAAC GCAGGAGGAA 
ACCAGTCCGA AGCCCCAGCT GACGGAGACA CAGTCATTCA GCCAGCAGGA GGAGCTCTCA 
CCCCGACAGG CCAGCGAGGA CACCAATGGA GCGCCCGCAG GAGCCTTGGA AATGCCAGTC 
CCAATGCCAC GCAAAATTAA CACAGTAGCA AAGAACAAAG CGAAGCGTGT GAAAACCATC 
TATGATTGCC AGGCAGACAA TGACGATGAG CTGACTTTTG TGGAGGGCGA GGTTATAATT 
GTCACAGGAG AGGAAGACCA GGAGTGGTGG ATCGGGCACA TAGAGGGTCA GCCTGAAAGG 
AAAGGGGTCT TCCCAATGTC CTTCGTGCAC ATTCTGTCAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212978.1
CDS	68..3532
Translation	MRSSSSRLSSFSSRDSLWSRMPDQISVSEFLSETTEDYNSPTTSSFTTRLQSCRNTVNVLEEALDQDRTALQKVKKSVKAIYNSGQEHVQNEENYGQALDKFGSNFISRDNSDLGTAFIKFSGLIKELAALLKNLLQSLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRIFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKESRRDSQSKQSGYSMHQLQGNKEFGSEKKGYLFKKSDGIRKVWQRRKCSVKNGILTISHATSNRQPVRLNLLTCQVKPSGEDKKCFDLISHNRTYHFQAEDEQEFVIWISVLTNSKEEALNMAFRGEQSAGDDSLEDLTKAIIEDVLRIPGNEVCCDCGAPEPKWLSTNLGILTCIECSGIHREMGVHISRIQSMELDKLGTSELLLAKNVGNSSFNEILEGNLPSPSPKPAPSSDMTERKEYINAKYVEHRFARRTATTATARQGDLYEAVRTRDLMALIQLYADGVELMDPFPEAGQDPGETALHFAVRTSDQTSLHLVDFLVQNSGTLDRQTESGNAALHYCCTYEKPECLKLLLRGKPSIDLVNQNGETALDIARRLRNVQCEELLVEAAAGRFNPHVHVEYEWNLRLEEIDESDDDLDDKPSPVKKERSPRPQSFCHSSSVSPQEKLTLPGYLGHRDKQRLSYGAFANPVYSTSTETPASPVSEGPTIASKTPAKAPSCGPPTSLPLGSQSSAGGSSTLSKKRAPPPPPGHKRTHSDPPSPVLQGPQSKGSESTPPSANRTSPANKFEGIQQQQSTTSMNTKATFGPRVLPKLPQKVALRKIDTIHLPSVDKSGPDVLQKPPQAQDAPPTRASDTITRPTEPPPKIPQVAERSQPVDVPQKPHISDLPPKPQLSDLPPKPQLSDLPPKPQLSDLPPKPQLKDLPPKPQISDLPSKPAVCSASEATQRQSTQEETSPKPQLTETQSFSQQEELSPRQASEDTNGAPAGALEMPVPMPRKINTVAKNKAKRVKTIYDCQADNDDELTFVEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPMSFVHILSD

Target ORF information:

RefSeq Version	XM_017357489.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1a (asap1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357489.1

ORF Insert Sequence:

ATGAGGTCCT CGTCCTCGCG TTTGTCAAGT TTTTCCTCCA GGGATTCATT ATGGAGTCGG 
ATGCCGGATC AGATCTCCGT GTCCGAGTTT CTCTCGGAGA CGACGGAGGA TTACAATTCC 
CCTACGACCT CGAGCTTCAC CACCCGCCTG CAGAGCTGCC GGAACACGGT CAATGTTCTG 
GAAGAGGCTT TGGATCAGGA CCGAACTGCT TTACAGAAGG TCAAGAAATC TGTCAAAGCA 
ATCTACAACT CGGGTCAAGA ACATGTGCAG AATGAAGAGA ATTATGGACA GGCACTGGAC 
AAGTTTGGCA GCAACTTCAT CAGCCGAGAT AACTCTGATC TGGGAACAGC CTTCATCAAG 
TTTTCTGGAC TTATCAAAGA GCTGGCTGCT CTCCTCAAGA ACCTGCTCCA GAGCCTCAGC 
CACAACGTCA TCTTCACCCT GGACTCTCTG CTCAAAGGAG ATCTAAAGGG AGTGAAGGGG 
GACCTTAAAA AGCCTTTTGA CAAGGCCTGG AAAGACTATG AAACCAAGTT CACAAAGATC 
GAGAAGGAGA AGAGAGAACA TGCCAAGCAG CACGGCATGA TCCGCACAGA GATCACCGGC 
GCAGAGATTG CAGAAGAGAT GGAGAAGGAG CGGAGGATCT TTCAGCTGCA GATGTGTGAG 
TACCTGATCA AAGTCAATGA GATTAAGACC AAGAAGGGAG TGGATCTCCT CCAGAATCTC 
ATCAAGTATT ATCATGCACA GTGCAATTTC TTCCAGGATG GCTTGAAAAC TGCTGACAAG 
TTGAAGCAGT ATATTGAAAA ATTAGCAGCT GATCTTTATA ATATAAAACA GACTCAGGAT 
GAGGAGAAAA AACAGCTCAC AGCTCTCAGA GACCTCATCA AATCTTCCTT ACAGCTGGAC 
CAGAAGGAGT CTAGGAGAGA TTCTCAGAGT AAGCAGAGCG GTTACAGCAT GCACCAGCTG 
CAGGGCAATA AGGAGTTTGG CAGTGAGAAG AAGGGCTATC TCTTTAAGAA GAGTGATGGG 
ATCCGTAAGG TGTGGCAGAG GAGGAAGTGC TCAGTGAAAA ATGGCATCCT CACCATCTCT 
CATGCCACAT CCAACAGGCA GCCGGTGAGA CTGAATCTGC TGACCTGCCA GGTTAAACCC 
AGTGGAGAGG ATAAGAAGTG CTTTGACCTC ATCTCTCATA ATCGAACATA TCATTTCCAG 
GCAGAGGACG AACAGGAGTT TGTGATATGG ATCTCGGTGC TGACTAATAG TAAGGAGGAG 
GCTCTGAACA TGGCATTTCG TGGGGAGCAG AGTGCTGGAG ATGACAGTTT GGAGGACTTG 
ACCAAAGCCA TCATCGAGGA CGTGCTGCGC ATTCCTGGAA ACGAAGTCTG CTGTGACTGT 
GGGGCTCCAG AGCCCAAATG GTTATCCACT AACCTCGGCA TCCTGACGTG CATCGAGTGT 
TCAGGAATCC ACAGGGAAAT GGGAGTCCAT ATTTCGCGCA TCCAATCCAT GGAGCTTGAC 
AAACTTGGAA CCTCTGAACT CTTGCTGGCT AAGAACGTGG GCAACAGTAG TTTCAACGAA 
ATATTAGAAG GGAATCTGCC GAGTCCTTCA CCAAAGCCAG CGCCATCAAG TGACATGACC 
GAGAGGAAGG AGTACATCAA TGCGAAGTAC GTGGAGCACA GGTTCGCTCG GCGAACGGCC 
ACTACAGCCA CAGCCAGACA GGGCGACTTG TACGAGGCGG TGAGAACGCG AGACTTGATG 
GCTCTCATTC AGCTCTATGC AGATGGAGTG GAGCTAATGG ATCCTTTCCC AGAAGCAGGA 
CAGGACCCGG GAGAGACAGC TCTGCACTTT GCTGTTCGGA CATCAGACCA GACTTCCCTG 
CACCTGGTGG ACTTTCTTGT CCAAAACAGT GGGACTCTAG ACAGACAGAC GGAGAGTGGA 
AACGCTGCTC TCCATTACTG CTGCACATAT GAGAAGCCAG AGTGTCTCAA ACTGCTGCTC 
AGGGGAAAAC CGTCTATTGA CCTGGTTAAT CAAAACGGGG AGACAGCATT GGATATCGCC 
AGACGACTGA GAAATGTACA GTGTGAAGAG CTACTGGTGG AGGCAGCAGC CGGGAGGTTT 
AATCCTCATG TGCATGTGGA GTATGAGTGG AATCTGCGGC TGGAGGAGAT TGATGAGAGT 
GACGATGACC TGGATGACAA GCCCAGTCCA GTGAAGAAGG AGCGTTCTCC TCGTCCTCAG 
AGCTTCTGTC ATTCGTCCAG CGTGTCTCCT CAGGAGAAGT TAACCCTGCC GGGGTATCTA 
GGACACAGGG ACAAGCAGAG ACTGTCCTAT GGAGCCTTTG CCAACCCCGT CTACAGCACC 
TCCACCGAAA CCCCTGCATC TCCAGTGTCA GAGGGACCCA CCATAGCCAG CAAGACCCCT 
GCAAAAGCTC CGTCCTGTGG GCCGCCCACC TCTCTGCCGC TGGGATCTCA ATCGAGTGCA 
GGAGGCAGCT CCACTCTGTC TAAGAAGAGA GCTCCTCCTC CACCTCCCGG ACACAAGCGC 
ACCCACTCAG ATCCCCCCAG TCCCGTACTG CAGGGTCCGC AGAGCAAAGG AAGTGAGTCC 
ACACCTCCTT CTGCAAATCG GACATCCCCG GCCAACAAGT TTGAGGGAAT CCAGCAGCAG 
CAAAGCACTA CGTCTATGAA CACAAAAGCA ACATTTGGCC CACGAGTTCT TCCCAAACTA 
CCTCAAAAAG TGGCACTACG AAAGATTGAC ACAATCCACC TCCCATCAGT GGACAAGTCT 
GGTCCTGATG TGCTTCAGAA ACCCCCACAG GCCCAGGATG CACCTCCCAC CAGAGCCTCA 
GATACAATAA CCAGACCCAC TGAACCTCCA CCTAAAATTC CACAGGTCGC AGAACGATCC 
CAGCCTGTGG ATGTCCCGCA GAAACCGCAC ATCTCAGACC TTCCTCCCAA ACCGCAACTA 
TCAGATCTTC CCCCCAAACC CCAATTGTCG GATTTACCAC CAAAACCTCA GCTTTCTGAC 
CTGCCCCCGA AGCCTCAGCT TAAGGATCTT CCCCCTAAGC CGCAGATCAG TGATCTGCCA 
TCCAAACCGG CCGTGTGTTC TGCATCTGAG GCCACACAAA GGCAGTCAAC GCAGGAGGAA 
ACCAGTCCGA AGCCCCAGCT GACGGAGACA CAGTCATTCA GCCAGCAGGA GGAGCTCTCA 
CCCCGACAGG CCAGCGAGGA CACCAATGGA GCGCCCGCAG GAGCCTTGGA AATGCCAGTC 
CCAATGCCAC GCAAAATTAA CACAGTAGCA AAGAACAAAG CGAAGCGTGT GAAAACCATC 
TATGATTGCC AGGCAGACAA TGACGATGAG CTGACTTTTG TGGAGGGCGA GGTTATAATT 
GTCACAGGAG AGGAAGACCA GGAGTGGTGG ATCGGGCACA TAGAGGGTCA GCCTGAAAGG 
AAAGGGGTCT TCCCAATGTC CTTCGTGCAC ATTCTGTCAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASAP1A ( XM_005163474.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005163531.1 Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1a (asap1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa07129
Clone ID Related Accession (Same CDS sequence)	XM_005163474.4 , XM_017357489.1 , XM_005163474.3
Accession Version	XM_005163474.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	arfGAP with SH3 domain, ankyrin repeat and PH domain 1a isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005163474.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTCCT CGTCCTCGCG TTTGTCAAGT TTTTCCTCCA GGGATTCATT ATGGAGTCGG 
ATGCCGGATC AGATCTCCGT GTCCGAGTTT CTCTCGGAGA CGACGGAGGA TTACAATTCC 
CCTACGACCT CGAGCTTCAC CACCCGCCTG CAGAGCTGCC GGAACACGGT CAATGTTCTG 
GAAGAGGCTT TGGATCAGGA CCGAACTGCT TTACAGAAGG TCAAGAAATC TGTCAAAGCA 
ATCTACAACT CGGGTCAAGA ACATGTGCAG AATGAAGAGA ATTATGGACA GGCACTGGAC 
AAGTTTGGCA GCAACTTCAT CAGCCGAGAT AACTCTGATC TGGGAACAGC CTTCATCAAG 
TTTTCTGGAC TTATCAAAGA GCTGGCTGCT CTCCTCAAGA ACCTGCTCCA GAGCCTCAGC 
CACAACGTCA TCTTCACCCT GGACTCTCTG CTCAAAGGAG ATCTAAAGGG AGTGAAGGGG 
GACCTTAAAA AGCCTTTTGA CAAGGCCTGG AAAGACTATG AAACCAAGTT CACAAAGATC 
GAGAAGGAGA AGAGAGAACA TGCCAAGCAG CACGGCATGA TCCGCACAGA GATCACCGGC 
GCAGAGATTG CAGAAGAGAT GGAGAAGGAG CGGAGGATCT TTCAGCTGCA GATGTGTGAG 
TACCTGATCA AAGTCAATGA GATTAAGACC AAGAAGGGAG TGGATCTCCT CCAGAATCTC 
ATCAAGTATT ATCATGCACA GTGCAATTTC TTCCAGGATG GCTTGAAAAC TGCTGACAAG 
TTGAAGCAGT ATATTGAAAA ATTAGCAGCT GATCTTTATA ATATAAAACA GACTCAGGAT 
GAGGAGAAAA AACAGCTCAC AGCTCTCAGA GACCTCATCA AATCTTCCTT ACAGCTGGAC 
CAGAAGGAGT CTAGGAGAGA TTCTCAGAGT AAGCAGAGCG GTTACAGCAT GCACCAGCTG 
CAGGGCAATA AGGAGTTTGG CAGTGAGAAG AAGGGCTATC TCTTTAAGAA GAGTGATGGG 
ATCCGTAAGG TGTGGCAGAG GAGGAAGTGC TCAGTGAAAA ATGGCATCCT CACCATCTCT 
CATGCCACAT CCAACAGGCA GCCGGTGAGA CTGAATCTGC TGACCTGCCA GGTTAAACCC 
AGTGGAGAGG ATAAGAAGTG CTTTGACCTC ATCTCTCATA ATCGAACATA TCATTTCCAG 
GCAGAGGACG AACAGGAGTT TGTGATATGG ATCTCGGTGC TGACTAATAG TAAGGAGGAG 
GCTCTGAACA TGGCATTTCG TGGGGAGCAG AGTGCTGGAG ATGACAGTTT GGAGGACTTG 
ACCAAAGCCA TCATCGAGGA CGTGCTGCGC ATTCCTGGAA ACGAAGTCTG CTGTGACTGT 
GGGGCTCCAG AGCCCAAATG GTTATCCACT AACCTCGGCA TCCTGACGTG CATCGAGTGT 
TCAGGAATCC ACAGGGAAAT GGGAGTCCAT ATTTCGCGCA TCCAATCCAT GGAGCTTGAC 
AAACTTGGAA CCTCTGAACT CTTGCTGGCT AAGAACGTGG GCAACAGTAG TTTCAACGAA 
ATATTAGAAG GGAATCTGCC GAGTCCTTCA CCAAAGCCAG CGCCATCAAG TGACATGACC 
GAGAGGAAGG AGTACATCAA TGCGAAGTAC GTGGAGCACA GGTTCGCTCG GCGAACGGCC 
ACTACAGCCA CAGCCAGACA GGGCGACTTG TACGAGGCGG TGAGAACGCG AGACTTGATG 
GCTCTCATTC AGCTCTATGC AGATGGAGTG GAGCTAATGG ATCCTTTCCC AGAAGCAGGA 
CAGGACCCGG GAGAGACAGC TCTGCACTTT GCTGTTCGGA CATCAGACCA GACTTCCCTG 
CACCTGGTGG ACTTTCTTGT CCAAAACAGT GGGACTCTAG ACAGACAGAC GGAGAGTGGA 
AACGCTGCTC TCCATTACTG CTGCACATAT GAGAAGCCAG AGTGTCTCAA ACTGCTGCTC 
AGGGGAAAAC CGTCTATTGA CCTGGTTAAT CAAAACGGGG AGACAGCATT GGATATCGCC 
AGACGACTGA GAAATGTACA GTGTGAAGAG CTACTGGTGG AGGCAGCAGC CGGGAGGTTT 
AATCCTCATG TGCATGTGGA GTATGAGTGG AATCTGCGGC TGGAGGAGAT TGATGAGAGT 
GACGATGACC TGGATGACAA GCCCAGTCCA GTGAAGAAGG AGCGTTCTCC TCGTCCTCAG 
AGCTTCTGTC ATTCGTCCAG CGTGTCTCCT CAGGAGAAGT TAACCCTGCC GGGGTATCTA 
GGACACAGGG ACAAGCAGAG ACTGTCCTAT GGAGCCTTTG CCAACCCCGT CTACAGCACC 
TCCACCGAAA CCCCTGCATC TCCAGTGTCA GAGGGACCCA CCATAGCCAG CAAGACCCCT 
GCAAAAGCTC CGTCCTGTGG GCCGCCCACC TCTCTGCCGC TGGGATCTCA ATCGAGTGCA 
GGAGGCAGCT CCACTCTGTC TAAGAAGAGA GCTCCTCCTC CACCTCCCGG ACACAAGCGC 
ACCCACTCAG ATCCCCCCAG TCCCGTACTG CAGGGTCCGC AGAGCAAAGG AAGTGAGTCC 
ACACCTCCTT CTGCAAATCG GACATCCCCG GCCAACAAGT TTGAGGGAAT CCAGCAGCAG 
CAAAGCACTA CGTCTATGAA CACAAAAGCA ACATTTGGCC CACGAGTTCT TCCCAAACTA 
CCTCAAAAAG TGGCACTACG AAAGATTGAC ACAATCCACC TCCCATCAGT GGACAAGTCT 
GGTCCTGATG TGCTTCAGAA ACCCCCACAG GCCCAGGATG CACCTCCCAC CAGAGCCTCA 
GATACAATAA CCAGACCCAC TGAACCTCCA CCTAAAATTC CACAGGTCGC AGAACGATCC 
CAGCCTGTGG ATGTCCCGCA GAAACCGCAC ATCTCAGACC TTCCTCCCAA ACCGCAACTA 
TCAGATCTTC CCCCCAAACC CCAATTGTCG GATTTACCAC CAAAACCTCA GCTTTCTGAC 
CTGCCCCCGA AGCCTCAGCT TAAGGATCTT CCCCCTAAGC CGCAGATCAG TGATCTGCCA 
TCCAAACCGG CCGTGTGTTC TGCATCTGAG GCCACACAAA GGCAGTCAAC GCAGGAGGAA 
ACCAGTCCGA AGCCCCAGCT GACGGAGACA CAGTCATTCA GCCAGCAGGA GGAGCTCTCA 
CCCCGACAGG CCAGCGAGGA CACCAATGGA GCGCCCGCAG GAGCCTTGGA AATGCCAGTC 
CCAATGCCAC GCAAAATTAA CACAGTAGCA AAGAACAAAG CGAAGCGTGT GAAAACCATC 
TATGATTGCC AGGCAGACAA TGACGATGAG CTGACTTTTG TGGAGGGCGA GGTTATAATT 
GTCACAGGAG AGGAAGACCA GGAGTGGTGG ATCGGGCACA TAGAGGGTCA GCCTGAAAGG 
AAAGGGGTCT TCCCAATGTC CTTCGTGCAC ATTCTGTCAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163531.1
CDS	289..3753
Translation	MRSSSSRLSSFSSRDSLWSRMPDQISVSEFLSETTEDYNSPTTSSFTTRLQSCRNTVNVLEEALDQDRTALQKVKKSVKAIYNSGQEHVQNEENYGQALDKFGSNFISRDNSDLGTAFIKFSGLIKELAALLKNLLQSLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRIFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKESRRDSQSKQSGYSMHQLQGNKEFGSEKKGYLFKKSDGIRKVWQRRKCSVKNGILTISHATSNRQPVRLNLLTCQVKPSGEDKKCFDLISHNRTYHFQAEDEQEFVIWISVLTNSKEEALNMAFRGEQSAGDDSLEDLTKAIIEDVLRIPGNEVCCDCGAPEPKWLSTNLGILTCIECSGIHREMGVHISRIQSMELDKLGTSELLLAKNVGNSSFNEILEGNLPSPSPKPAPSSDMTERKEYINAKYVEHRFARRTATTATARQGDLYEAVRTRDLMALIQLYADGVELMDPFPEAGQDPGETALHFAVRTSDQTSLHLVDFLVQNSGTLDRQTESGNAALHYCCTYEKPECLKLLLRGKPSIDLVNQNGETALDIARRLRNVQCEELLVEAAAGRFNPHVHVEYEWNLRLEEIDESDDDLDDKPSPVKKERSPRPQSFCHSSSVSPQEKLTLPGYLGHRDKQRLSYGAFANPVYSTSTETPASPVSEGPTIASKTPAKAPSCGPPTSLPLGSQSSAGGSSTLSKKRAPPPPPGHKRTHSDPPSPVLQGPQSKGSESTPPSANRTSPANKFEGIQQQQSTTSMNTKATFGPRVLPKLPQKVALRKIDTIHLPSVDKSGPDVLQKPPQAQDAPPTRASDTITRPTEPPPKIPQVAERSQPVDVPQKPHISDLPPKPQLSDLPPKPQLSDLPPKPQLSDLPPKPQLKDLPPKPQISDLPSKPAVCSASEATQRQSTQEETSPKPQLTETQSFSQQEELSPRQASEDTNGAPAGALEMPVPMPRKINTVAKNKAKRVKTIYDCQADNDDELTFVEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPMSFVHILSD

Target ORF information:

RefSeq Version	XM_005163474.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1a (asap1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163474.3

ORF Insert Sequence:

ATGAGGTCCT CGTCCTCGCG TTTGTCAAGT TTTTCCTCCA GGGATTCATT ATGGAGTCGG 
ATGCCGGATC AGATCTCCGT GTCCGAGTTT CTCTCGGAGA CGACGGAGGA TTACAATTCC 
CCTACGACCT CGAGCTTCAC CACCCGCCTG CAGAGCTGCC GGAACACGGT CAATGTTCTG 
GAAGAGGCTT TGGATCAGGA CCGAACTGCT TTACAGAAGG TCAAGAAATC TGTCAAAGCA 
ATCTACAACT CGGGTCAAGA ACATGTGCAG AATGAAGAGA ATTATGGACA GGCACTGGAC 
AAGTTTGGCA GCAACTTCAT CAGCCGAGAT AACTCTGATC TGGGAACAGC CTTCATCAAG 
TTTTCTGGAC TTATCAAAGA GCTGGCTGCT CTCCTCAAGA ACCTGCTCCA GAGCCTCAGC 
CACAACGTCA TCTTCACCCT GGACTCTCTG CTCAAAGGAG ATCTAAAGGG AGTGAAGGGG 
GACCTTAAAA AGCCTTTTGA CAAGGCCTGG AAAGACTATG AAACCAAGTT CACAAAGATC 
GAGAAGGAGA AGAGAGAACA TGCCAAGCAG CACGGCATGA TCCGCACAGA GATCACCGGC 
GCAGAGATTG CAGAAGAGAT GGAGAAGGAG CGGAGGATCT TTCAGCTGCA GATGTGTGAG 
TACCTGATCA AAGTCAATGA GATTAAGACC AAGAAGGGAG TGGATCTCCT CCAGAATCTC 
ATCAAGTATT ATCATGCACA GTGCAATTTC TTCCAGGATG GCTTGAAAAC TGCTGACAAG 
TTGAAGCAGT ATATTGAAAA ATTAGCAGCT GATCTTTATA ATATAAAACA GACTCAGGAT 
GAGGAGAAAA AACAGCTCAC AGCTCTCAGA GACCTCATCA AATCTTCCTT ACAGCTGGAC 
CAGAAGGAGT CTAGGAGAGA TTCTCAGAGT AAGCAGAGCG GTTACAGCAT GCACCAGCTG 
CAGGGCAATA AGGAGTTTGG CAGTGAGAAG AAGGGCTATC TCTTTAAGAA GAGTGATGGG 
ATCCGTAAGG TGTGGCAGAG GAGGAAGTGC TCAGTGAAAA ATGGCATCCT CACCATCTCT 
CATGCCACAT CCAACAGGCA GCCGGTGAGA CTGAATCTGC TGACCTGCCA GGTTAAACCC 
AGTGGAGAGG ATAAGAAGTG CTTTGACCTC ATCTCTCATA ATCGAACATA TCATTTCCAG 
GCAGAGGACG AACAGGAGTT TGTGATATGG ATCTCGGTGC TGACTAATAG TAAGGAGGAG 
GCTCTGAACA TGGCATTTCG TGGGGAGCAG AGTGCTGGAG ATGACAGTTT GGAGGACTTG 
ACCAAAGCCA TCATCGAGGA CGTGCTGCGC ATTCCTGGAA ACGAAGTCTG CTGTGACTGT 
GGGGCTCCAG AGCCCAAATG GTTATCCACT AACCTCGGCA TCCTGACGTG CATCGAGTGT 
TCAGGAATCC ACAGGGAAAT GGGAGTCCAT ATTTCGCGCA TCCAATCCAT GGAGCTTGAC 
AAACTTGGAA CCTCTGAACT CTTGCTGGCT AAGAACGTGG GCAACAGTAG TTTCAACGAA 
ATATTAGAAG GGAATCTGCC GAGTCCTTCA CCAAAGCCAG CGCCATCAAG TGACATGACC 
GAGAGGAAGG AGTACATCAA TGCGAAGTAC GTGGAGCACA GGTTCGCTCG GCGAACGGCC 
ACTACAGCCA CAGCCAGACA GGGCGACTTG TACGAGGCGG TGAGAACGCG AGACTTGATG 
GCTCTCATTC AGCTCTATGC AGATGGAGTG GAGCTAATGG ATCCTTTCCC AGAAGCAGGA 
CAGGACCCGG GAGAGACAGC TCTGCACTTT GCTGTTCGGA CATCAGACCA GACTTCCCTG 
CACCTGGTGG ACTTTCTTGT CCAAAACAGT GGGACTCTAG ACAGACAGAC GGAGAGTGGA 
AACGCTGCTC TCCATTACTG CTGCACATAT GAGAAGCCAG AGTGTCTCAA ACTGCTGCTC 
AGGGGAAAAC CGTCTATTGA CCTGGTTAAT CAAAACGGGG AGACAGCATT GGATATCGCC 
AGACGACTGA GAAATGTACA GTGTGAAGAG CTACTGGTGG AGGCAGCAGC CGGGAGGTTT 
AATCCTCATG TGCATGTGGA GTATGAGTGG AATCTGCGGC TGGAGGAGAT TGATGAGAGT 
GACGATGACC TGGATGACAA GCCCAGTCCA GTGAAGAAGG AGCGTTCTCC TCGTCCTCAG 
AGCTTCTGTC ATTCGTCCAG CGTGTCTCCT CAGGAGAAGT TAACCCTGCC GGGGTATCTA 
GGACACAGGG ACAAGCAGAG ACTGTCCTAT GGAGCCTTTG CCAACCCCGT CTACAGCACC 
TCCACCGAAA CCCCTGCATC TCCAGTGTCA GAGGGACCCA CCATAGCCAG CAAGACCCCT 
GCAAAAGCTC CGTCCTGTGG GCCGCCCACC TCTCTGCCGC TGGGATCTCA ATCGAGTGCA 
GGAGGCAGCT CCACTCTGTC TAAGAAGAGA GCTCCTCCTC CACCTCCCGG ACACAAGCGC 
ACCCACTCAG ATCCCCCCAG TCCCGTACTG CAGGGTCCGC AGAGCAAAGG AAGTGAGTCC 
ACACCTCCTT CTGCAAATCG GACATCCCCG GCCAACAAGT TTGAGGGAAT CCAGCAGCAG 
CAAAGCACTA CGTCTATGAA CACAAAAGCA ACATTTGGCC CACGAGTTCT TCCCAAACTA 
CCTCAAAAAG TGGCACTACG AAAGATTGAC ACAATCCACC TCCCATCAGT GGACAAGTCT 
GGTCCTGATG TGCTTCAGAA ACCCCCACAG GCCCAGGATG CACCTCCCAC CAGAGCCTCA 
GATACAATAA CCAGACCCAC TGAACCTCCA CCTAAAATTC CACAGGTCGC AGAACGATCC 
CAGCCTGTGG ATGTCCCGCA GAAACCGCAC ATCTCAGACC TTCCTCCCAA ACCGCAACTA 
TCAGATCTTC CCCCCAAACC CCAATTGTCG GATTTACCAC CAAAACCTCA GCTTTCTGAC 
CTGCCCCCGA AGCCTCAGCT TAAGGATCTT CCCCCTAAGC CGCAGATCAG TGATCTGCCA 
TCCAAACCGG CCGTGTGTTC TGCATCTGAG GCCACACAAA GGCAGTCAAC GCAGGAGGAA 
ACCAGTCCGA AGCCCCAGCT GACGGAGACA CAGTCATTCA GCCAGCAGGA GGAGCTCTCA 
CCCCGACAGG CCAGCGAGGA CACCAATGGA GCGCCCGCAG GAGCCTTGGA AATGCCAGTC 
CCAATGCCAC GCAAAATTAA CACAGTAGCA AAGAACAAAG CGAAGCGTGT GAAAACCATC 
TATGATTGCC AGGCAGACAA TGACGATGAG CTGACTTTTG TGGAGGGCGA GGTTATAATT 
GTCACAGGAG AGGAAGACCA GGAGTGGTGG ATCGGGCACA TAGAGGGTCA GCCTGAAAGG 
AAAGGGGTCT TCCCAATGTC CTTCGTGCAC ATTCTGTCAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASAP1A ( XM_005163475.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005163532.1 Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1a (asap1a), transcript variant X2, mRNA.

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CloneID	ODa07130
Clone ID Related Accession (Same CDS sequence)	XM_005163475.4 , XM_005163475.3
Accession Version	XM_005163475.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	arfGAP with SH3 domain, ankyrin repeat and PH domain 1a isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005163475.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTCCT CGTCCTCGCG TTTGTCAAGT TTTTCCTCCA GGGATTCATT ATGGAGTCGG 
ATGCCGGATC AGATCTCCGT GTCCGAGTTT CTCTCGGAGA CGACGGAGGA TTACAATTCC 
CCTACGACCT CGAGCTTCAC CACCCGCCTG CAGAGCTGCC GGAACACGGT CAATGTTCTG 
GAAGAGGCTT TGGATCAGGA CCGAACTGCT TTACAGAAGG TCAAGAAATC TGTCAAAGCA 
ATCTACAACT CGGGTCAAGA ACATGTGCAG AATGAAGAGA ATTATGGACA GGCACTGGAC 
AAGTTTGGCA GCAACTTCAT CAGCCGAGAT AACTCTGATC TGGGAACAGC CTTCATCAAG 
TTTTCTGGAC TTATCAAAGA GCTGGCTGCT CTCCTCAAGA ACCTGCTCCA GAGCCTCAGC 
CACAACGTCA TCTTCACCCT GGACTCTCTG CTCAAAGGAG ATCTAAAGGG AGTGAAGGGG 
GACCTTAAAA AGCCTTTTGA CAAGGCCTGG AAAGACTATG AAACCAAGTT CACAAAGATC 
GAGAAGGAGA AGAGAGAACA TGCCAAGCAG CACGGCATGA TCCGCACAGA GATCACCGGC 
GCAGAGATTG CAGAAGAGAT GGAGAAGGAG CGGAGGATCT TTCAGCTGCA GATGTGTGAG 
TACCTGATCA AAGTCAATGA GATTAAGACC AAGAAGGGAG TGGATCTCCT CCAGAATCTC 
ATCAAGTATT ATCATGCACA GTGCAATTTC TTCCAGGATG GCTTGAAAAC TGCTGACAAG 
TTGAAGCAGT ATATTGAAAA ATTAGCAGCT GATCTTTATA ATATAAAACA GACTCAGGAT 
GAGGAGAAAA AACAGCTCAC AGCTCTCAGA GACCTCATCA AATCTTCCTT ACAGCTGGAC 
CAGAAGGAGG ATTCTCAGAG TAAGCAGAGC GGTTACAGCA TGCACCAGCT GCAGGGCAAT 
AAGGAGTTTG GCAGTGAGAA GAAGGGCTAT CTCTTTAAGA AGAGTGATGG GATCCGTAAG 
GTGTGGCAGA GGAGGAAGTG CTCAGTGAAA AATGGCATCC TCACCATCTC TCATGCCACA 
TCCAACAGGC AGCCGGTGAG ACTGAATCTG CTGACCTGCC AGGTTAAACC CAGTGGAGAG 
GATAAGAAGT GCTTTGACCT CATCTCTCAT AATCGAACAT ATCATTTCCA GGCAGAGGAC 
GAACAGGAGT TTGTGATATG GATCTCGGTG CTGACTAATA GTAAGGAGGA GGCTCTGAAC 
ATGGCATTTC GTGGGGAGCA GAGTGCTGGA GATGACAGTT TGGAGGACTT GACCAAAGCC 
ATCATCGAGG ACGTGCTGCG CATTCCTGGA AACGAAGTCT GCTGTGACTG TGGGGCTCCA 
GAGCCCAAAT GGTTATCCAC TAACCTCGGC ATCCTGACGT GCATCGAGTG TTCAGGAATC 
CACAGGGAAA TGGGAGTCCA TATTTCGCGC ATCCAATCCA TGGAGCTTGA CAAACTTGGA 
ACCTCTGAAC TCTTGCTGGC TAAGAACGTG GGCAACAGTA GTTTCAACGA AATATTAGAA 
GGGAATCTGC CGAGTCCTTC ACCAAAGCCA GCGCCATCAA GTGACATGAC CGAGAGGAAG 
GAGTACATCA ATGCGAAGTA CGTGGAGCAC AGGTTCGCTC GGCGAACGGC CACTACAGCC 
ACAGCCAGAC AGGGCGACTT GTACGAGGCG GTGAGAACGC GAGACTTGAT GGCTCTCATT 
CAGCTCTATG CAGATGGAGT GGAGCTAATG GATCCTTTCC CAGAAGCAGG ACAGGACCCG 
GGAGAGACAG CTCTGCACTT TGCTGTTCGG ACATCAGACC AGACTTCCCT GCACCTGGTG 
GACTTTCTTG TCCAAAACAG TGGGACTCTA GACAGACAGA CGGAGAGTGG AAACGCTGCT 
CTCCATTACT GCTGCACATA TGAGAAGCCA GAGTGTCTCA AACTGCTGCT CAGGGGAAAA 
CCGTCTATTG ACCTGGTTAA TCAAAACGGG GAGACAGCAT TGGATATCGC CAGACGACTG 
AGAAATGTAC AGTGTGAAGA GCTACTGGTG GAGGCAGCAG CCGGGAGGTT TAATCCTCAT 
GTGCATGTGG AGTATGAGTG GAATCTGCGG CTGGAGGAGA TTGATGAGAG TGACGATGAC 
CTGGATGACA AGCCCAGTCC AGTGAAGAAG GAGCGTTCTC CTCGTCCTCA GAGCTTCTGT 
CATTCGTCCA GCGTGTCTCC TCAGGAGAAG TTAACCCTGC CGGGGTATCT AGGACACAGG 
GACAAGCAGA GACTGTCCTA TGGAGCCTTT GCCAACCCCG TCTACAGCAC CTCCACCGAA 
ACCCCTGCAT CTCCAGTGTC AGAGGGACCC ACCATAGCCA GCAAGACCCC TGCAAAAGCT 
CCGTCCTGTG GGCCGCCCAC CTCTCTGCCG CTGGGATCTC AATCGAGTGC AGGAGGCAGC 
TCCACTCTGT CTAAGAAGAG AGCTCCTCCT CCACCTCCCG GACACAAGCG CACCCACTCA 
GATCCCCCCA GTCCCGTACT GCAGGGTCCG CAGAGCAAAG GAAGTGAGTC CACACCTCCT 
TCTGCAAATC GGACATCCCC GGCCAACAAG TTTGAGGGAA TCCAGCAGCA GCAAAGCACT 
ACGTCTATGA ACACAAAAGC AACATTTGGC CCACGAGTTC TTCCCAAACT ACCTCAAAAA 
GTGGCACTAC GAAAGATTGA CACAATCCAC CTCCCATCAG TGGACAAGTC TGGTCCTGAT 
GTGCTTCAGA AACCCCCACA GGCCCAGGAT GCACCTCCCA CCAGAGCCTC AGATACAATA 
ACCAGACCCA CTGAACCTCC ACCTAAAATT CCACAGGTCG CAGAACGATC CCAGCCTGTG 
GATGTCCCGC AGAAACCGCA CATCTCAGAC CTTCCTCCCA AACCGCAACT ATCAGATCTT 
CCCCCCAAAC CCCAATTGTC GGATTTACCA CCAAAACCTC AGCTTTCTGA CCTGCCCCCG 
AAGCCTCAGC TTAAGGATCT TCCCCCTAAG CCGCAGATCA GTGATCTGCC ATCCAAACCG 
GCCGTGTGTT CTGCATCTGA GGCCACACAA AGGCAGTCAA CGCAGGAGGA AACCAGTCCG 
AAGCCCCAGC TGACGGAGAC ACAGTCATTC AGCCAGCAGG AGGAGCTCTC ACCCCGACAG 
GCCAGCGAGG ACACCAATGG AGCGCCCGCA GGAGCCTTGG AAATGCCAGT CCCAATGCCA 
CGCAAAATTA ACACAGTAGC AAAGAACAAA GCGAAGCGTG TGAAAACCAT CTATGATTGC 
CAGGCAGACA ATGACGATGA GCTGACTTTT GTGGAGGGCG AGGTTATAAT TGTCACAGGA 
GAGGAAGACC AGGAGTGGTG GATCGGGCAC ATAGAGGGTC AGCCTGAAAG GAAAGGGGTC 
TTCCCAATGT CCTTCGTGCA CATTCTGTCA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163532.1
CDS	275..3730
Translation	MRSSSSRLSSFSSRDSLWSRMPDQISVSEFLSETTEDYNSPTTSSFTTRLQSCRNTVNVLEEALDQDRTALQKVKKSVKAIYNSGQEHVQNEENYGQALDKFGSNFISRDNSDLGTAFIKFSGLIKELAALLKNLLQSLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRIFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKEDSQSKQSGYSMHQLQGNKEFGSEKKGYLFKKSDGIRKVWQRRKCSVKNGILTISHATSNRQPVRLNLLTCQVKPSGEDKKCFDLISHNRTYHFQAEDEQEFVIWISVLTNSKEEALNMAFRGEQSAGDDSLEDLTKAIIEDVLRIPGNEVCCDCGAPEPKWLSTNLGILTCIECSGIHREMGVHISRIQSMELDKLGTSELLLAKNVGNSSFNEILEGNLPSPSPKPAPSSDMTERKEYINAKYVEHRFARRTATTATARQGDLYEAVRTRDLMALIQLYADGVELMDPFPEAGQDPGETALHFAVRTSDQTSLHLVDFLVQNSGTLDRQTESGNAALHYCCTYEKPECLKLLLRGKPSIDLVNQNGETALDIARRLRNVQCEELLVEAAAGRFNPHVHVEYEWNLRLEEIDESDDDLDDKPSPVKKERSPRPQSFCHSSSVSPQEKLTLPGYLGHRDKQRLSYGAFANPVYSTSTETPASPVSEGPTIASKTPAKAPSCGPPTSLPLGSQSSAGGSSTLSKKRAPPPPPGHKRTHSDPPSPVLQGPQSKGSESTPPSANRTSPANKFEGIQQQQSTTSMNTKATFGPRVLPKLPQKVALRKIDTIHLPSVDKSGPDVLQKPPQAQDAPPTRASDTITRPTEPPPKIPQVAERSQPVDVPQKPHISDLPPKPQLSDLPPKPQLSDLPPKPQLSDLPPKPQLKDLPPKPQISDLPSKPAVCSASEATQRQSTQEETSPKPQLTETQSFSQQEELSPRQASEDTNGAPAGALEMPVPMPRKINTVAKNKAKRVKTIYDCQADNDDELTFVEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPMSFVHILSD

Target ORF information:

RefSeq Version	XM_005163475.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1a (asap1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163475.4

ORF Insert Sequence:

ATGAGGTCCT CGTCCTCGCG TTTGTCAAGT TTTTCCTCCA GGGATTCATT ATGGAGTCGG 
ATGCCGGATC AGATCTCCGT GTCCGAGTTT CTCTCGGAGA CGACGGAGGA TTACAATTCC 
CCTACGACCT CGAGCTTCAC CACCCGCCTG CAGAGCTGCC GGAACACGGT CAATGTTCTG 
GAAGAGGCTT TGGATCAGGA CCGAACTGCT TTACAGAAGG TCAAGAAATC TGTCAAAGCA 
ATCTACAACT CGGGTCAAGA ACATGTGCAG AATGAAGAGA ATTATGGACA GGCACTGGAC 
AAGTTTGGCA GCAACTTCAT CAGCCGAGAT AACTCTGATC TGGGAACAGC CTTCATCAAG 
TTTTCTGGAC TTATCAAAGA GCTGGCTGCT CTCCTCAAGA ACCTGCTCCA GAGCCTCAGC 
CACAACGTCA TCTTCACCCT GGACTCTCTG CTCAAAGGAG ATCTAAAGGG AGTGAAGGGG 
GACCTTAAAA AGCCTTTTGA CAAGGCCTGG AAAGACTATG AAACCAAGTT CACAAAGATC 
GAGAAGGAGA AGAGAGAACA TGCCAAGCAG CACGGCATGA TCCGCACAGA GATCACCGGC 
GCAGAGATTG CAGAAGAGAT GGAGAAGGAG CGGAGGATCT TTCAGCTGCA GATGTGTGAG 
TACCTGATCA AAGTCAATGA GATTAAGACC AAGAAGGGAG TGGATCTCCT CCAGAATCTC 
ATCAAGTATT ATCATGCACA GTGCAATTTC TTCCAGGATG GCTTGAAAAC TGCTGACAAG 
TTGAAGCAGT ATATTGAAAA ATTAGCAGCT GATCTTTATA ATATAAAACA GACTCAGGAT 
GAGGAGAAAA AACAGCTCAC AGCTCTCAGA GACCTCATCA AATCTTCCTT ACAGCTGGAC 
CAGAAGGAGG ATTCTCAGAG TAAGCAGAGC GGTTACAGCA TGCACCAGCT GCAGGGCAAT 
AAGGAGTTTG GCAGTGAGAA GAAGGGCTAT CTCTTTAAGA AGAGTGATGG GATCCGTAAG 
GTGTGGCAGA GGAGGAAGTG CTCAGTGAAA AATGGCATCC TCACCATCTC TCATGCCACA 
TCCAACAGGC AGCCGGTGAG ACTGAATCTG CTGACCTGCC AGGTTAAACC CAGTGGAGAG 
GATAAGAAGT GCTTTGACCT CATCTCTCAT AATCGAACAT ATCATTTCCA GGCAGAGGAC 
GAACAGGAGT TTGTGATATG GATCTCGGTG CTGACTAATA GTAAGGAGGA GGCTCTGAAC 
ATGGCATTTC GTGGGGAGCA GAGTGCTGGA GATGACAGTT TGGAGGACTT GACCAAAGCC 
ATCATCGAGG ACGTGCTGCG CATTCCTGGA AACGAAGTCT GCTGTGACTG TGGGGCTCCA 
GAGCCCAAAT GGTTATCCAC TAACCTCGGC ATCCTGACGT GCATCGAGTG TTCAGGAATC 
CACAGGGAAA TGGGAGTCCA TATTTCGCGC ATCCAATCCA TGGAGCTTGA CAAACTTGGA 
ACCTCTGAAC TCTTGCTGGC TAAGAACGTG GGCAACAGTA GTTTCAACGA AATATTAGAA 
GGGAATCTGC CGAGTCCTTC ACCAAAGCCA GCGCCATCAA GTGACATGAC CGAGAGGAAG 
GAGTACATCA ATGCGAAGTA CGTGGAGCAC AGGTTCGCTC GGCGAACGGC CACTACAGCC 
ACAGCCAGAC AGGGCGACTT GTACGAGGCG GTGAGAACGC GAGACTTGAT GGCTCTCATT 
CAGCTCTATG CAGATGGAGT GGAGCTAATG GATCCTTTCC CAGAAGCAGG ACAGGACCCG 
GGAGAGACAG CTCTGCACTT TGCTGTTCGG ACATCAGACC AGACTTCCCT GCACCTGGTG 
GACTTTCTTG TCCAAAACAG TGGGACTCTA GACAGACAGA CGGAGAGTGG AAACGCTGCT 
CTCCATTACT GCTGCACATA TGAGAAGCCA GAGTGTCTCA AACTGCTGCT CAGGGGAAAA 
CCGTCTATTG ACCTGGTTAA TCAAAACGGG GAGACAGCAT TGGATATCGC CAGACGACTG 
AGAAATGTAC AGTGTGAAGA GCTACTGGTG GAGGCAGCAG CCGGGAGGTT TAATCCTCAT 
GTGCATGTGG AGTATGAGTG GAATCTGCGG CTGGAGGAGA TTGATGAGAG TGACGATGAC 
CTGGATGACA AGCCCAGTCC AGTGAAGAAG GAGCGTTCTC CTCGTCCTCA GAGCTTCTGT 
CATTCGTCCA GCGTGTCTCC TCAGGAGAAG TTAACCCTGC CGGGGTATCT AGGACACAGG 
GACAAGCAGA GACTGTCCTA TGGAGCCTTT GCCAACCCCG TCTACAGCAC CTCCACCGAA 
ACCCCTGCAT CTCCAGTGTC AGAGGGACCC ACCATAGCCA GCAAGACCCC TGCAAAAGCT 
CCGTCCTGTG GGCCGCCCAC CTCTCTGCCG CTGGGATCTC AATCGAGTGC AGGAGGCAGC 
TCCACTCTGT CTAAGAAGAG AGCTCCTCCT CCACCTCCCG GACACAAGCG CACCCACTCA 
GATCCCCCCA GTCCCGTACT GCAGGGTCCG CAGAGCAAAG GAAGTGAGTC CACACCTCCT 
TCTGCAAATC GGACATCCCC GGCCAACAAG TTTGAGGGAA TCCAGCAGCA GCAAAGCACT 
ACGTCTATGA ACACAAAAGC AACATTTGGC CCACGAGTTC TTCCCAAACT ACCTCAAAAA 
GTGGCACTAC GAAAGATTGA CACAATCCAC CTCCCATCAG TGGACAAGTC TGGTCCTGAT 
GTGCTTCAGA AACCCCCACA GGCCCAGGAT GCACCTCCCA CCAGAGCCTC AGATACAATA 
ACCAGACCCA CTGAACCTCC ACCTAAAATT CCACAGGTCG CAGAACGATC CCAGCCTGTG 
GATGTCCCGC AGAAACCGCA CATCTCAGAC CTTCCTCCCA AACCGCAACT ATCAGATCTT 
CCCCCCAAAC CCCAATTGTC GGATTTACCA CCAAAACCTC AGCTTTCTGA CCTGCCCCCG 
AAGCCTCAGC TTAAGGATCT TCCCCCTAAG CCGCAGATCA GTGATCTGCC ATCCAAACCG 
GCCGTGTGTT CTGCATCTGA GGCCACACAA AGGCAGTCAA CGCAGGAGGA AACCAGTCCG 
AAGCCCCAGC TGACGGAGAC ACAGTCATTC AGCCAGCAGG AGGAGCTCTC ACCCCGACAG 
GCCAGCGAGG ACACCAATGG AGCGCCCGCA GGAGCCTTGG AAATGCCAGT CCCAATGCCA 
CGCAAAATTA ACACAGTAGC AAAGAACAAA GCGAAGCGTG TGAAAACCAT CTATGATTGC 
CAGGCAGACA ATGACGATGA GCTGACTTTT GTGGAGGGCG AGGTTATAAT TGTCACAGGA 
GAGGAAGACC AGGAGTGGTG GATCGGGCAC ATAGAGGGTC AGCCTGAAAG GAAAGGGGTC 
TTCCCAATGT CCTTCGTGCA CATTCTGTCA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASAP1A ( XM_005163475.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005163532.1 Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1a (asap1a), transcript variant X3, mRNA.

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CloneID	ODa07130
Clone ID Related Accession (Same CDS sequence)	XM_005163475.4 , XM_005163475.3
Accession Version	XM_005163475.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	arfGAP with SH3 domain, ankyrin repeat and PH domain 1a isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005163475.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTCCT CGTCCTCGCG TTTGTCAAGT TTTTCCTCCA GGGATTCATT ATGGAGTCGG 
ATGCCGGATC AGATCTCCGT GTCCGAGTTT CTCTCGGAGA CGACGGAGGA TTACAATTCC 
CCTACGACCT CGAGCTTCAC CACCCGCCTG CAGAGCTGCC GGAACACGGT CAATGTTCTG 
GAAGAGGCTT TGGATCAGGA CCGAACTGCT TTACAGAAGG TCAAGAAATC TGTCAAAGCA 
ATCTACAACT CGGGTCAAGA ACATGTGCAG AATGAAGAGA ATTATGGACA GGCACTGGAC 
AAGTTTGGCA GCAACTTCAT CAGCCGAGAT AACTCTGATC TGGGAACAGC CTTCATCAAG 
TTTTCTGGAC TTATCAAAGA GCTGGCTGCT CTCCTCAAGA ACCTGCTCCA GAGCCTCAGC 
CACAACGTCA TCTTCACCCT GGACTCTCTG CTCAAAGGAG ATCTAAAGGG AGTGAAGGGG 
GACCTTAAAA AGCCTTTTGA CAAGGCCTGG AAAGACTATG AAACCAAGTT CACAAAGATC 
GAGAAGGAGA AGAGAGAACA TGCCAAGCAG CACGGCATGA TCCGCACAGA GATCACCGGC 
GCAGAGATTG CAGAAGAGAT GGAGAAGGAG CGGAGGATCT TTCAGCTGCA GATGTGTGAG 
TACCTGATCA AAGTCAATGA GATTAAGACC AAGAAGGGAG TGGATCTCCT CCAGAATCTC 
ATCAAGTATT ATCATGCACA GTGCAATTTC TTCCAGGATG GCTTGAAAAC TGCTGACAAG 
TTGAAGCAGT ATATTGAAAA ATTAGCAGCT GATCTTTATA ATATAAAACA GACTCAGGAT 
GAGGAGAAAA AACAGCTCAC AGCTCTCAGA GACCTCATCA AATCTTCCTT ACAGCTGGAC 
CAGAAGGAGG ATTCTCAGAG TAAGCAGAGC GGTTACAGCA TGCACCAGCT GCAGGGCAAT 
AAGGAGTTTG GCAGTGAGAA GAAGGGCTAT CTCTTTAAGA AGAGTGATGG GATCCGTAAG 
GTGTGGCAGA GGAGGAAGTG CTCAGTGAAA AATGGCATCC TCACCATCTC TCATGCCACA 
TCCAACAGGC AGCCGGTGAG ACTGAATCTG CTGACCTGCC AGGTTAAACC CAGTGGAGAG 
GATAAGAAGT GCTTTGACCT CATCTCTCAT AATCGAACAT ATCATTTCCA GGCAGAGGAC 
GAACAGGAGT TTGTGATATG GATCTCGGTG CTGACTAATA GTAAGGAGGA GGCTCTGAAC 
ATGGCATTTC GTGGGGAGCA GAGTGCTGGA GATGACAGTT TGGAGGACTT GACCAAAGCC 
ATCATCGAGG ACGTGCTGCG CATTCCTGGA AACGAAGTCT GCTGTGACTG TGGGGCTCCA 
GAGCCCAAAT GGTTATCCAC TAACCTCGGC ATCCTGACGT GCATCGAGTG TTCAGGAATC 
CACAGGGAAA TGGGAGTCCA TATTTCGCGC ATCCAATCCA TGGAGCTTGA CAAACTTGGA 
ACCTCTGAAC TCTTGCTGGC TAAGAACGTG GGCAACAGTA GTTTCAACGA AATATTAGAA 
GGGAATCTGC CGAGTCCTTC ACCAAAGCCA GCGCCATCAA GTGACATGAC CGAGAGGAAG 
GAGTACATCA ATGCGAAGTA CGTGGAGCAC AGGTTCGCTC GGCGAACGGC CACTACAGCC 
ACAGCCAGAC AGGGCGACTT GTACGAGGCG GTGAGAACGC GAGACTTGAT GGCTCTCATT 
CAGCTCTATG CAGATGGAGT GGAGCTAATG GATCCTTTCC CAGAAGCAGG ACAGGACCCG 
GGAGAGACAG CTCTGCACTT TGCTGTTCGG ACATCAGACC AGACTTCCCT GCACCTGGTG 
GACTTTCTTG TCCAAAACAG TGGGACTCTA GACAGACAGA CGGAGAGTGG AAACGCTGCT 
CTCCATTACT GCTGCACATA TGAGAAGCCA GAGTGTCTCA AACTGCTGCT CAGGGGAAAA 
CCGTCTATTG ACCTGGTTAA TCAAAACGGG GAGACAGCAT TGGATATCGC CAGACGACTG 
AGAAATGTAC AGTGTGAAGA GCTACTGGTG GAGGCAGCAG CCGGGAGGTT TAATCCTCAT 
GTGCATGTGG AGTATGAGTG GAATCTGCGG CTGGAGGAGA TTGATGAGAG TGACGATGAC 
CTGGATGACA AGCCCAGTCC AGTGAAGAAG GAGCGTTCTC CTCGTCCTCA GAGCTTCTGT 
CATTCGTCCA GCGTGTCTCC TCAGGAGAAG TTAACCCTGC CGGGGTATCT AGGACACAGG 
GACAAGCAGA GACTGTCCTA TGGAGCCTTT GCCAACCCCG TCTACAGCAC CTCCACCGAA 
ACCCCTGCAT CTCCAGTGTC AGAGGGACCC ACCATAGCCA GCAAGACCCC TGCAAAAGCT 
CCGTCCTGTG GGCCGCCCAC CTCTCTGCCG CTGGGATCTC AATCGAGTGC AGGAGGCAGC 
TCCACTCTGT CTAAGAAGAG AGCTCCTCCT CCACCTCCCG GACACAAGCG CACCCACTCA 
GATCCCCCCA GTCCCGTACT GCAGGGTCCG CAGAGCAAAG GAAGTGAGTC CACACCTCCT 
TCTGCAAATC GGACATCCCC GGCCAACAAG TTTGAGGGAA TCCAGCAGCA GCAAAGCACT 
ACGTCTATGA ACACAAAAGC AACATTTGGC CCACGAGTTC TTCCCAAACT ACCTCAAAAA 
GTGGCACTAC GAAAGATTGA CACAATCCAC CTCCCATCAG TGGACAAGTC TGGTCCTGAT 
GTGCTTCAGA AACCCCCACA GGCCCAGGAT GCACCTCCCA CCAGAGCCTC AGATACAATA 
ACCAGACCCA CTGAACCTCC ACCTAAAATT CCACAGGTCG CAGAACGATC CCAGCCTGTG 
GATGTCCCGC AGAAACCGCA CATCTCAGAC CTTCCTCCCA AACCGCAACT ATCAGATCTT 
CCCCCCAAAC CCCAATTGTC GGATTTACCA CCAAAACCTC AGCTTTCTGA CCTGCCCCCG 
AAGCCTCAGC TTAAGGATCT TCCCCCTAAG CCGCAGATCA GTGATCTGCC ATCCAAACCG 
GCCGTGTGTT CTGCATCTGA GGCCACACAA AGGCAGTCAA CGCAGGAGGA AACCAGTCCG 
AAGCCCCAGC TGACGGAGAC ACAGTCATTC AGCCAGCAGG AGGAGCTCTC ACCCCGACAG 
GCCAGCGAGG ACACCAATGG AGCGCCCGCA GGAGCCTTGG AAATGCCAGT CCCAATGCCA 
CGCAAAATTA ACACAGTAGC AAAGAACAAA GCGAAGCGTG TGAAAACCAT CTATGATTGC 
CAGGCAGACA ATGACGATGA GCTGACTTTT GTGGAGGGCG AGGTTATAAT TGTCACAGGA 
GAGGAAGACC AGGAGTGGTG GATCGGGCAC ATAGAGGGTC AGCCTGAAAG GAAAGGGGTC 
TTCCCAATGT CCTTCGTGCA CATTCTGTCA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163532.1
CDS	289..3744
Translation	MRSSSSRLSSFSSRDSLWSRMPDQISVSEFLSETTEDYNSPTTSSFTTRLQSCRNTVNVLEEALDQDRTALQKVKKSVKAIYNSGQEHVQNEENYGQALDKFGSNFISRDNSDLGTAFIKFSGLIKELAALLKNLLQSLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRIFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKEDSQSKQSGYSMHQLQGNKEFGSEKKGYLFKKSDGIRKVWQRRKCSVKNGILTISHATSNRQPVRLNLLTCQVKPSGEDKKCFDLISHNRTYHFQAEDEQEFVIWISVLTNSKEEALNMAFRGEQSAGDDSLEDLTKAIIEDVLRIPGNEVCCDCGAPEPKWLSTNLGILTCIECSGIHREMGVHISRIQSMELDKLGTSELLLAKNVGNSSFNEILEGNLPSPSPKPAPSSDMTERKEYINAKYVEHRFARRTATTATARQGDLYEAVRTRDLMALIQLYADGVELMDPFPEAGQDPGETALHFAVRTSDQTSLHLVDFLVQNSGTLDRQTESGNAALHYCCTYEKPECLKLLLRGKPSIDLVNQNGETALDIARRLRNVQCEELLVEAAAGRFNPHVHVEYEWNLRLEEIDESDDDLDDKPSPVKKERSPRPQSFCHSSSVSPQEKLTLPGYLGHRDKQRLSYGAFANPVYSTSTETPASPVSEGPTIASKTPAKAPSCGPPTSLPLGSQSSAGGSSTLSKKRAPPPPPGHKRTHSDPPSPVLQGPQSKGSESTPPSANRTSPANKFEGIQQQQSTTSMNTKATFGPRVLPKLPQKVALRKIDTIHLPSVDKSGPDVLQKPPQAQDAPPTRASDTITRPTEPPPKIPQVAERSQPVDVPQKPHISDLPPKPQLSDLPPKPQLSDLPPKPQLSDLPPKPQLKDLPPKPQISDLPSKPAVCSASEATQRQSTQEETSPKPQLTETQSFSQQEELSPRQASEDTNGAPAGALEMPVPMPRKINTVAKNKAKRVKTIYDCQADNDDELTFVEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPMSFVHILSD

Target ORF information:

RefSeq Version	XM_005163475.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1a (asap1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163475.3

ORF Insert Sequence:

ATGAGGTCCT CGTCCTCGCG TTTGTCAAGT TTTTCCTCCA GGGATTCATT ATGGAGTCGG 
ATGCCGGATC AGATCTCCGT GTCCGAGTTT CTCTCGGAGA CGACGGAGGA TTACAATTCC 
CCTACGACCT CGAGCTTCAC CACCCGCCTG CAGAGCTGCC GGAACACGGT CAATGTTCTG 
GAAGAGGCTT TGGATCAGGA CCGAACTGCT TTACAGAAGG TCAAGAAATC TGTCAAAGCA 
ATCTACAACT CGGGTCAAGA ACATGTGCAG AATGAAGAGA ATTATGGACA GGCACTGGAC 
AAGTTTGGCA GCAACTTCAT CAGCCGAGAT AACTCTGATC TGGGAACAGC CTTCATCAAG 
TTTTCTGGAC TTATCAAAGA GCTGGCTGCT CTCCTCAAGA ACCTGCTCCA GAGCCTCAGC 
CACAACGTCA TCTTCACCCT GGACTCTCTG CTCAAAGGAG ATCTAAAGGG AGTGAAGGGG 
GACCTTAAAA AGCCTTTTGA CAAGGCCTGG AAAGACTATG AAACCAAGTT CACAAAGATC 
GAGAAGGAGA AGAGAGAACA TGCCAAGCAG CACGGCATGA TCCGCACAGA GATCACCGGC 
GCAGAGATTG CAGAAGAGAT GGAGAAGGAG CGGAGGATCT TTCAGCTGCA GATGTGTGAG 
TACCTGATCA AAGTCAATGA GATTAAGACC AAGAAGGGAG TGGATCTCCT CCAGAATCTC 
ATCAAGTATT ATCATGCACA GTGCAATTTC TTCCAGGATG GCTTGAAAAC TGCTGACAAG 
TTGAAGCAGT ATATTGAAAA ATTAGCAGCT GATCTTTATA ATATAAAACA GACTCAGGAT 
GAGGAGAAAA AACAGCTCAC AGCTCTCAGA GACCTCATCA AATCTTCCTT ACAGCTGGAC 
CAGAAGGAGG ATTCTCAGAG TAAGCAGAGC GGTTACAGCA TGCACCAGCT GCAGGGCAAT 
AAGGAGTTTG GCAGTGAGAA GAAGGGCTAT CTCTTTAAGA AGAGTGATGG GATCCGTAAG 
GTGTGGCAGA GGAGGAAGTG CTCAGTGAAA AATGGCATCC TCACCATCTC TCATGCCACA 
TCCAACAGGC AGCCGGTGAG ACTGAATCTG CTGACCTGCC AGGTTAAACC CAGTGGAGAG 
GATAAGAAGT GCTTTGACCT CATCTCTCAT AATCGAACAT ATCATTTCCA GGCAGAGGAC 
GAACAGGAGT TTGTGATATG GATCTCGGTG CTGACTAATA GTAAGGAGGA GGCTCTGAAC 
ATGGCATTTC GTGGGGAGCA GAGTGCTGGA GATGACAGTT TGGAGGACTT GACCAAAGCC 
ATCATCGAGG ACGTGCTGCG CATTCCTGGA AACGAAGTCT GCTGTGACTG TGGGGCTCCA 
GAGCCCAAAT GGTTATCCAC TAACCTCGGC ATCCTGACGT GCATCGAGTG TTCAGGAATC 
CACAGGGAAA TGGGAGTCCA TATTTCGCGC ATCCAATCCA TGGAGCTTGA CAAACTTGGA 
ACCTCTGAAC TCTTGCTGGC TAAGAACGTG GGCAACAGTA GTTTCAACGA AATATTAGAA 
GGGAATCTGC CGAGTCCTTC ACCAAAGCCA GCGCCATCAA GTGACATGAC CGAGAGGAAG 
GAGTACATCA ATGCGAAGTA CGTGGAGCAC AGGTTCGCTC GGCGAACGGC CACTACAGCC 
ACAGCCAGAC AGGGCGACTT GTACGAGGCG GTGAGAACGC GAGACTTGAT GGCTCTCATT 
CAGCTCTATG CAGATGGAGT GGAGCTAATG GATCCTTTCC CAGAAGCAGG ACAGGACCCG 
GGAGAGACAG CTCTGCACTT TGCTGTTCGG ACATCAGACC AGACTTCCCT GCACCTGGTG 
GACTTTCTTG TCCAAAACAG TGGGACTCTA GACAGACAGA CGGAGAGTGG AAACGCTGCT 
CTCCATTACT GCTGCACATA TGAGAAGCCA GAGTGTCTCA AACTGCTGCT CAGGGGAAAA 
CCGTCTATTG ACCTGGTTAA TCAAAACGGG GAGACAGCAT TGGATATCGC CAGACGACTG 
AGAAATGTAC AGTGTGAAGA GCTACTGGTG GAGGCAGCAG CCGGGAGGTT TAATCCTCAT 
GTGCATGTGG AGTATGAGTG GAATCTGCGG CTGGAGGAGA TTGATGAGAG TGACGATGAC 
CTGGATGACA AGCCCAGTCC AGTGAAGAAG GAGCGTTCTC CTCGTCCTCA GAGCTTCTGT 
CATTCGTCCA GCGTGTCTCC TCAGGAGAAG TTAACCCTGC CGGGGTATCT AGGACACAGG 
GACAAGCAGA GACTGTCCTA TGGAGCCTTT GCCAACCCCG TCTACAGCAC CTCCACCGAA 
ACCCCTGCAT CTCCAGTGTC AGAGGGACCC ACCATAGCCA GCAAGACCCC TGCAAAAGCT 
CCGTCCTGTG GGCCGCCCAC CTCTCTGCCG CTGGGATCTC AATCGAGTGC AGGAGGCAGC 
TCCACTCTGT CTAAGAAGAG AGCTCCTCCT CCACCTCCCG GACACAAGCG CACCCACTCA 
GATCCCCCCA GTCCCGTACT GCAGGGTCCG CAGAGCAAAG GAAGTGAGTC CACACCTCCT 
TCTGCAAATC GGACATCCCC GGCCAACAAG TTTGAGGGAA TCCAGCAGCA GCAAAGCACT 
ACGTCTATGA ACACAAAAGC AACATTTGGC CCACGAGTTC TTCCCAAACT ACCTCAAAAA 
GTGGCACTAC GAAAGATTGA CACAATCCAC CTCCCATCAG TGGACAAGTC TGGTCCTGAT 
GTGCTTCAGA AACCCCCACA GGCCCAGGAT GCACCTCCCA CCAGAGCCTC AGATACAATA 
ACCAGACCCA CTGAACCTCC ACCTAAAATT CCACAGGTCG CAGAACGATC CCAGCCTGTG 
GATGTCCCGC AGAAACCGCA CATCTCAGAC CTTCCTCCCA AACCGCAACT ATCAGATCTT 
CCCCCCAAAC CCCAATTGTC GGATTTACCA CCAAAACCTC AGCTTTCTGA CCTGCCCCCG 
AAGCCTCAGC TTAAGGATCT TCCCCCTAAG CCGCAGATCA GTGATCTGCC ATCCAAACCG 
GCCGTGTGTT CTGCATCTGA GGCCACACAA AGGCAGTCAA CGCAGGAGGA AACCAGTCCG 
AAGCCCCAGC TGACGGAGAC ACAGTCATTC AGCCAGCAGG AGGAGCTCTC ACCCCGACAG 
GCCAGCGAGG ACACCAATGG AGCGCCCGCA GGAGCCTTGG AAATGCCAGT CCCAATGCCA 
CGCAAAATTA ACACAGTAGC AAAGAACAAA GCGAAGCGTG TGAAAACCAT CTATGATTGC 
CAGGCAGACA ATGACGATGA GCTGACTTTT GTGGAGGGCG AGGTTATAAT TGTCACAGGA 
GAGGAAGACC AGGAGTGGTG GATCGGGCAC ATAGAGGGTC AGCCTGAAAG GAAAGGGGTC 
TTCCCAATGT CCTTCGTGCA CATTCTGTCA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASAP1A ( XM_005163477.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005163534.1 Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1a (asap1a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa07132
Clone ID Related Accession (Same CDS sequence)	XM_005163477.4 , XM_005163477.3
Accession Version	XM_005163477.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	arfGAP with SH3 domain, ankyrin repeat and PH domain 1a isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005163477.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTCCT CGTCCTCGCG TTTGTCAAGT TTTTCCTCCA GGGATTCATT ATGGAGTCGG 
ATGCCGGATC AGATCTCCGT GTCCGAGTTT CTCTCGGAGA CGACGGAGGA TTACAATTCC 
CCTACGACCT CGAGCTTCAC CACCCGCCTG CAGAGCTGCC GGAACACGGT CAATGTTCTG 
GAAGAGGCTT TGGATCAGGA CCGAACTGCT TTACAGAAGG TCAAGAAATC TGTCAAAGCA 
ATCTACAACT CGGGTCAAGA ACATGTGCAG AATGAAGAGA ATTATGGACA GGCACTGGAC 
AAGTTTGGCA GCAACTTCAT CAGCCGAGAT AACTCTGATC TGGGAACAGC CTTCATCAAG 
TTTTCTGGAC TTATCAAAGA GCTGGCTGCT CTCCTCAAGA ACCTGCTCCA GAGCCTCAGC 
CACAACGTCA TCTTCACCCT GGACTCTCTG CTCAAAGGAG ATCTAAAGGG AGTGAAGGGG 
GACCTTAAAA AGCCTTTTGA CAAGGCCTGG AAAGACTATG AAACCAAGTT CACAAAGATC 
GAGAAGGAGA AGAGAGAACA TGCCAAGCAG CACGGCATGA TCCGCACAGA GATCACCGGC 
GCAGAGATTG CAGAAGAGAT GGAGAAGGAG CGGAGGATCT TTCAGCTGCA GATGTGTGAG 
TACCTGATCA AAGTCAATGA GATTAAGACC AAGAAGGGAG TGGATCTCCT CCAGAATCTC 
ATCAAGTATT ATCATGCACA GTGCAATTTC TTCCAGGATG GCTTGAAAAC TGCTGACAAG 
TTGAAGCAGT ATATTGAAAA ATTAGCAGCT GATCTTTATA ATATAAAACA GACTCAGGAT 
GAGGAGAAAA AACAGCTCAC AGCTCTCAGA GACCTCATCA AATCTTCCTT ACAGCTGGAC 
CAGAAGGAGT CTAGGAGAGA TTCTCAGAGT AAGCAGAGCG GTTACAGCAT GCACCAGCTG 
CAGGGCAATA AGGAGTTTGG CAGTGAGAAG AAGGGCTATC TCTTTAAGAA GAGTGATGGG 
ATCCGTAAGG TGTGGCAGAG GAGGAAGTGC TCAGTGAAAA ATGGCATCCT CACCATCTCT 
CATGCCACAT CCAACAGGCA GCCGGTGAGA CTGAATCTGC TGACCTGCCA GGTTAAACCC 
AGTGGAGAGG ATAAGAAGTG CTTTGACCTC ATCTCTCATA ATCGAACATA TCATTTCCAG 
GCAGAGGACG AACAGGAGTT TGTGATATGG ATCTCGGTGC TGACTAATAG TAAGGAGGAG 
GCTCTGAACA TGGCATTTCG TGGGGAGCAG AGTGCTGGAG ATGACAGTTT GGAGGACTTG 
ACCAAAGCCA TCATCGAGGA CGTGCTGCGC ATTCCTGGAA ACGAAGTCTG CTGTGACTGT 
GGGGCTCCAG AGCCCAAATG GTTATCCACT AACCTCGGCA TCCTGACGTG CATCGAGTGT 
TCAGGAATCC ACAGGGAAAT GGGAGTCCAT ATTTCGCGCA TCCAATCCAT GGAGCTTGAC 
AAACTTGGAA CCTCTGAACT CTTGCTGGCT AAGAACGTGG GCAACAGTAG TTTCAACGAA 
ATATTAGAAG GGAATCTGCC GAGTCCTTCA CCAAAGCCAG CGCCATCAAG TGACATGACC 
GAGAGGAAGG AGTACATCAA TGCGAAGTAC GTGGAGCACA GGTTCGCTCG GCGAACGGCC 
ACTACAGCCA CAGCCAGACA GGGCGACTTG TACGAGGCGG TGAGAACGCG AGACTTGATG 
GCTCTCATTC AGCTCTATGC AGATGGAGTG GAGCTAATGG ATCCTTTCCC AGAAGCAGGA 
CAGGACCCGG GAGAGACAGC TCTGCACTTT GCTGTTCGGA CATCAGACCA GACTTCCCTG 
CACCTGGTGG ACTTTCTTGT CCAAAACAGT GGGACTCTAG ACAGACAGAC GGAGAGTGGA 
AACGCTGCTC TCCATTACTG CTGCACATAT GAGAAGCCAG AGTGTCTCAA ACTGCTGCTC 
AGGGGAAAAC CGTCTATTGA CCTGGTTAAT CAAAACGGGG AGACAGCATT GGATATCGCC 
AGACGACTGA GAAATGTACA GTGTGAAGAG CTACTGGTGG AGGCAGCAGC CGGGAGGTTT 
AATCCTCATG TGCATGTGGA GTATGAGTGG AATCTGCGGC TGGAGGAGAT TGATGAGAGT 
GACGATGACC TGGATGACAA GCCCAGTCCA GTGAAGAAGG AGCGTTCTCC TCGTCCTCAG 
AGCTTCTGTC ATTCGTCCAG CGTGTCTCCT CAGGAGAAGT TAACCCTGCC GGGGTATCTA 
GGACACAGGG ACAAGCAGAG ACTGTCCTAT GGAGCCTTTG CCAACCCCGT CTACAGCACC 
TCCACCGAAA CCCCTGCATC TCCAGTGTCA GAGGGACCCA CCATAGCCAG CAAGACCCCT 
GCAAAAGGAA GTGAGTCCAC ACCTCCTTCT GCAAATCGGA CATCCCCGGC CAACAAGTTT 
GAGGGAATCC AGCAGCAGCA AAGCACTACG TCTATGAACA CAAAAGCAAC ATTTGGCCCA 
CGAGTTCTTC CCAAACTACC TCAAAAAGTG GCACTACGAA AGATTGACAC AATCCACCTC 
CCATCAGTGG ACAAGTCTGG TCCTGATGTG CTTCAGAAAC CCCCACAGGC CCAGGATGCA 
CCTCCCACCA GAGCCTCAGA TACAATAACC AGACCCACTG AACCTCCACC TAAAATTCCA 
CAGGTCGCAG AACGATCCCA GCCTGTGGAT GTCCCGCAGA AACCGCACAT CTCAGACCTT 
CCTCCCAAAC CGCAACTATC AGATCTTCCC CCCAAACCCC AATTGTCGGA TTTACCACCA 
AAACCTCAGC TTTCTGACCT GCCCCCGAAG CCTCAGCTTA AGGATCTTCC CCCTAAGCCG 
CAGATCAGTG ATCTGCCATC CAAACCGGCC GTGTGTTCTG CATCTGAGGC CACACAAAGG 
CAGTCAACGC AGGAGGAAAC CAGTCCGAAG CCCCAGCTGA CGGAGACACA GTCATTCAGC 
CAGCAGGAGG AGCTCTCACC CCGACAGGCC AGCGAGGACA CCAATGGAGC GCCCGCAGGA 
GCCTTGGAAA TGCCAGTCCC AATGCCACGC AAAATTAACA CAGTAGCAAA GAACAAAGCG 
AAGCGTGTGA AAACCATCTA TGATTGCCAG GCAGACAATG ACGATGAGCT GACTTTTGTG 
GAGGGCGAGG TTATAATTGT CACAGGAGAG GAAGACCAGG AGTGGTGGAT CGGGCACATA 
GAGGGTCAGC CTGAAAGGAA AGGGGTCTTC CCAATGTCCT TCGTGCACAT TCTGTCAGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163534.1
CDS	276..3578
Translation	MRSSSSRLSSFSSRDSLWSRMPDQISVSEFLSETTEDYNSPTTSSFTTRLQSCRNTVNVLEEALDQDRTALQKVKKSVKAIYNSGQEHVQNEENYGQALDKFGSNFISRDNSDLGTAFIKFSGLIKELAALLKNLLQSLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRIFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKESRRDSQSKQSGYSMHQLQGNKEFGSEKKGYLFKKSDGIRKVWQRRKCSVKNGILTISHATSNRQPVRLNLLTCQVKPSGEDKKCFDLISHNRTYHFQAEDEQEFVIWISVLTNSKEEALNMAFRGEQSAGDDSLEDLTKAIIEDVLRIPGNEVCCDCGAPEPKWLSTNLGILTCIECSGIHREMGVHISRIQSMELDKLGTSELLLAKNVGNSSFNEILEGNLPSPSPKPAPSSDMTERKEYINAKYVEHRFARRTATTATARQGDLYEAVRTRDLMALIQLYADGVELMDPFPEAGQDPGETALHFAVRTSDQTSLHLVDFLVQNSGTLDRQTESGNAALHYCCTYEKPECLKLLLRGKPSIDLVNQNGETALDIARRLRNVQCEELLVEAAAGRFNPHVHVEYEWNLRLEEIDESDDDLDDKPSPVKKERSPRPQSFCHSSSVSPQEKLTLPGYLGHRDKQRLSYGAFANPVYSTSTETPASPVSEGPTIASKTPAKGSESTPPSANRTSPANKFEGIQQQQSTTSMNTKATFGPRVLPKLPQKVALRKIDTIHLPSVDKSGPDVLQKPPQAQDAPPTRASDTITRPTEPPPKIPQVAERSQPVDVPQKPHISDLPPKPQLSDLPPKPQLSDLPPKPQLSDLPPKPQLKDLPPKPQISDLPSKPAVCSASEATQRQSTQEETSPKPQLTETQSFSQQEELSPRQASEDTNGAPAGALEMPVPMPRKINTVAKNKAKRVKTIYDCQADNDDELTFVEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPMSFVHILSD

Target ORF information:

RefSeq Version	XM_005163477.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1a (asap1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163477.4

ORF Insert Sequence:

ATGAGGTCCT CGTCCTCGCG TTTGTCAAGT TTTTCCTCCA GGGATTCATT ATGGAGTCGG 
ATGCCGGATC AGATCTCCGT GTCCGAGTTT CTCTCGGAGA CGACGGAGGA TTACAATTCC 
CCTACGACCT CGAGCTTCAC CACCCGCCTG CAGAGCTGCC GGAACACGGT CAATGTTCTG 
GAAGAGGCTT TGGATCAGGA CCGAACTGCT TTACAGAAGG TCAAGAAATC TGTCAAAGCA 
ATCTACAACT CGGGTCAAGA ACATGTGCAG AATGAAGAGA ATTATGGACA GGCACTGGAC 
AAGTTTGGCA GCAACTTCAT CAGCCGAGAT AACTCTGATC TGGGAACAGC CTTCATCAAG 
TTTTCTGGAC TTATCAAAGA GCTGGCTGCT CTCCTCAAGA ACCTGCTCCA GAGCCTCAGC 
CACAACGTCA TCTTCACCCT GGACTCTCTG CTCAAAGGAG ATCTAAAGGG AGTGAAGGGG 
GACCTTAAAA AGCCTTTTGA CAAGGCCTGG AAAGACTATG AAACCAAGTT CACAAAGATC 
GAGAAGGAGA AGAGAGAACA TGCCAAGCAG CACGGCATGA TCCGCACAGA GATCACCGGC 
GCAGAGATTG CAGAAGAGAT GGAGAAGGAG CGGAGGATCT TTCAGCTGCA GATGTGTGAG 
TACCTGATCA AAGTCAATGA GATTAAGACC AAGAAGGGAG TGGATCTCCT CCAGAATCTC 
ATCAAGTATT ATCATGCACA GTGCAATTTC TTCCAGGATG GCTTGAAAAC TGCTGACAAG 
TTGAAGCAGT ATATTGAAAA ATTAGCAGCT GATCTTTATA ATATAAAACA GACTCAGGAT 
GAGGAGAAAA AACAGCTCAC AGCTCTCAGA GACCTCATCA AATCTTCCTT ACAGCTGGAC 
CAGAAGGAGT CTAGGAGAGA TTCTCAGAGT AAGCAGAGCG GTTACAGCAT GCACCAGCTG 
CAGGGCAATA AGGAGTTTGG CAGTGAGAAG AAGGGCTATC TCTTTAAGAA GAGTGATGGG 
ATCCGTAAGG TGTGGCAGAG GAGGAAGTGC TCAGTGAAAA ATGGCATCCT CACCATCTCT 
CATGCCACAT CCAACAGGCA GCCGGTGAGA CTGAATCTGC TGACCTGCCA GGTTAAACCC 
AGTGGAGAGG ATAAGAAGTG CTTTGACCTC ATCTCTCATA ATCGAACATA TCATTTCCAG 
GCAGAGGACG AACAGGAGTT TGTGATATGG ATCTCGGTGC TGACTAATAG TAAGGAGGAG 
GCTCTGAACA TGGCATTTCG TGGGGAGCAG AGTGCTGGAG ATGACAGTTT GGAGGACTTG 
ACCAAAGCCA TCATCGAGGA CGTGCTGCGC ATTCCTGGAA ACGAAGTCTG CTGTGACTGT 
GGGGCTCCAG AGCCCAAATG GTTATCCACT AACCTCGGCA TCCTGACGTG CATCGAGTGT 
TCAGGAATCC ACAGGGAAAT GGGAGTCCAT ATTTCGCGCA TCCAATCCAT GGAGCTTGAC 
AAACTTGGAA CCTCTGAACT CTTGCTGGCT AAGAACGTGG GCAACAGTAG TTTCAACGAA 
ATATTAGAAG GGAATCTGCC GAGTCCTTCA CCAAAGCCAG CGCCATCAAG TGACATGACC 
GAGAGGAAGG AGTACATCAA TGCGAAGTAC GTGGAGCACA GGTTCGCTCG GCGAACGGCC 
ACTACAGCCA CAGCCAGACA GGGCGACTTG TACGAGGCGG TGAGAACGCG AGACTTGATG 
GCTCTCATTC AGCTCTATGC AGATGGAGTG GAGCTAATGG ATCCTTTCCC AGAAGCAGGA 
CAGGACCCGG GAGAGACAGC TCTGCACTTT GCTGTTCGGA CATCAGACCA GACTTCCCTG 
CACCTGGTGG ACTTTCTTGT CCAAAACAGT GGGACTCTAG ACAGACAGAC GGAGAGTGGA 
AACGCTGCTC TCCATTACTG CTGCACATAT GAGAAGCCAG AGTGTCTCAA ACTGCTGCTC 
AGGGGAAAAC CGTCTATTGA CCTGGTTAAT CAAAACGGGG AGACAGCATT GGATATCGCC 
AGACGACTGA GAAATGTACA GTGTGAAGAG CTACTGGTGG AGGCAGCAGC CGGGAGGTTT 
AATCCTCATG TGCATGTGGA GTATGAGTGG AATCTGCGGC TGGAGGAGAT TGATGAGAGT 
GACGATGACC TGGATGACAA GCCCAGTCCA GTGAAGAAGG AGCGTTCTCC TCGTCCTCAG 
AGCTTCTGTC ATTCGTCCAG CGTGTCTCCT CAGGAGAAGT TAACCCTGCC GGGGTATCTA 
GGACACAGGG ACAAGCAGAG ACTGTCCTAT GGAGCCTTTG CCAACCCCGT CTACAGCACC 
TCCACCGAAA CCCCTGCATC TCCAGTGTCA GAGGGACCCA CCATAGCCAG CAAGACCCCT 
GCAAAAGGAA GTGAGTCCAC ACCTCCTTCT GCAAATCGGA CATCCCCGGC CAACAAGTTT 
GAGGGAATCC AGCAGCAGCA AAGCACTACG TCTATGAACA CAAAAGCAAC ATTTGGCCCA 
CGAGTTCTTC CCAAACTACC TCAAAAAGTG GCACTACGAA AGATTGACAC AATCCACCTC 
CCATCAGTGG ACAAGTCTGG TCCTGATGTG CTTCAGAAAC CCCCACAGGC CCAGGATGCA 
CCTCCCACCA GAGCCTCAGA TACAATAACC AGACCCACTG AACCTCCACC TAAAATTCCA 
CAGGTCGCAG AACGATCCCA GCCTGTGGAT GTCCCGCAGA AACCGCACAT CTCAGACCTT 
CCTCCCAAAC CGCAACTATC AGATCTTCCC CCCAAACCCC AATTGTCGGA TTTACCACCA 
AAACCTCAGC TTTCTGACCT GCCCCCGAAG CCTCAGCTTA AGGATCTTCC CCCTAAGCCG 
CAGATCAGTG ATCTGCCATC CAAACCGGCC GTGTGTTCTG CATCTGAGGC CACACAAAGG 
CAGTCAACGC AGGAGGAAAC CAGTCCGAAG CCCCAGCTGA CGGAGACACA GTCATTCAGC 
CAGCAGGAGG AGCTCTCACC CCGACAGGCC AGCGAGGACA CCAATGGAGC GCCCGCAGGA 
GCCTTGGAAA TGCCAGTCCC AATGCCACGC AAAATTAACA CAGTAGCAAA GAACAAAGCG 
AAGCGTGTGA AAACCATCTA TGATTGCCAG GCAGACAATG ACGATGAGCT GACTTTTGTG 
GAGGGCGAGG TTATAATTGT CACAGGAGAG GAAGACCAGG AGTGGTGGAT CGGGCACATA 
GAGGGTCAGC CTGAAAGGAA AGGGGTCTTC CCAATGTCCT TCGTGCACAT TCTGTCAGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASAP1A ( XM_005163477.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005163534.1 Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1a (asap1a), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa07132
Clone ID Related Accession (Same CDS sequence)	XM_005163477.4 , XM_005163477.3
Accession Version	XM_005163477.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	arfGAP with SH3 domain, ankyrin repeat and PH domain 1a isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005163477.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTCCT CGTCCTCGCG TTTGTCAAGT TTTTCCTCCA GGGATTCATT ATGGAGTCGG 
ATGCCGGATC AGATCTCCGT GTCCGAGTTT CTCTCGGAGA CGACGGAGGA TTACAATTCC 
CCTACGACCT CGAGCTTCAC CACCCGCCTG CAGAGCTGCC GGAACACGGT CAATGTTCTG 
GAAGAGGCTT TGGATCAGGA CCGAACTGCT TTACAGAAGG TCAAGAAATC TGTCAAAGCA 
ATCTACAACT CGGGTCAAGA ACATGTGCAG AATGAAGAGA ATTATGGACA GGCACTGGAC 
AAGTTTGGCA GCAACTTCAT CAGCCGAGAT AACTCTGATC TGGGAACAGC CTTCATCAAG 
TTTTCTGGAC TTATCAAAGA GCTGGCTGCT CTCCTCAAGA ACCTGCTCCA GAGCCTCAGC 
CACAACGTCA TCTTCACCCT GGACTCTCTG CTCAAAGGAG ATCTAAAGGG AGTGAAGGGG 
GACCTTAAAA AGCCTTTTGA CAAGGCCTGG AAAGACTATG AAACCAAGTT CACAAAGATC 
GAGAAGGAGA AGAGAGAACA TGCCAAGCAG CACGGCATGA TCCGCACAGA GATCACCGGC 
GCAGAGATTG CAGAAGAGAT GGAGAAGGAG CGGAGGATCT TTCAGCTGCA GATGTGTGAG 
TACCTGATCA AAGTCAATGA GATTAAGACC AAGAAGGGAG TGGATCTCCT CCAGAATCTC 
ATCAAGTATT ATCATGCACA GTGCAATTTC TTCCAGGATG GCTTGAAAAC TGCTGACAAG 
TTGAAGCAGT ATATTGAAAA ATTAGCAGCT GATCTTTATA ATATAAAACA GACTCAGGAT 
GAGGAGAAAA AACAGCTCAC AGCTCTCAGA GACCTCATCA AATCTTCCTT ACAGCTGGAC 
CAGAAGGAGT CTAGGAGAGA TTCTCAGAGT AAGCAGAGCG GTTACAGCAT GCACCAGCTG 
CAGGGCAATA AGGAGTTTGG CAGTGAGAAG AAGGGCTATC TCTTTAAGAA GAGTGATGGG 
ATCCGTAAGG TGTGGCAGAG GAGGAAGTGC TCAGTGAAAA ATGGCATCCT CACCATCTCT 
CATGCCACAT CCAACAGGCA GCCGGTGAGA CTGAATCTGC TGACCTGCCA GGTTAAACCC 
AGTGGAGAGG ATAAGAAGTG CTTTGACCTC ATCTCTCATA ATCGAACATA TCATTTCCAG 
GCAGAGGACG AACAGGAGTT TGTGATATGG ATCTCGGTGC TGACTAATAG TAAGGAGGAG 
GCTCTGAACA TGGCATTTCG TGGGGAGCAG AGTGCTGGAG ATGACAGTTT GGAGGACTTG 
ACCAAAGCCA TCATCGAGGA CGTGCTGCGC ATTCCTGGAA ACGAAGTCTG CTGTGACTGT 
GGGGCTCCAG AGCCCAAATG GTTATCCACT AACCTCGGCA TCCTGACGTG CATCGAGTGT 
TCAGGAATCC ACAGGGAAAT GGGAGTCCAT ATTTCGCGCA TCCAATCCAT GGAGCTTGAC 
AAACTTGGAA CCTCTGAACT CTTGCTGGCT AAGAACGTGG GCAACAGTAG TTTCAACGAA 
ATATTAGAAG GGAATCTGCC GAGTCCTTCA CCAAAGCCAG CGCCATCAAG TGACATGACC 
GAGAGGAAGG AGTACATCAA TGCGAAGTAC GTGGAGCACA GGTTCGCTCG GCGAACGGCC 
ACTACAGCCA CAGCCAGACA GGGCGACTTG TACGAGGCGG TGAGAACGCG AGACTTGATG 
GCTCTCATTC AGCTCTATGC AGATGGAGTG GAGCTAATGG ATCCTTTCCC AGAAGCAGGA 
CAGGACCCGG GAGAGACAGC TCTGCACTTT GCTGTTCGGA CATCAGACCA GACTTCCCTG 
CACCTGGTGG ACTTTCTTGT CCAAAACAGT GGGACTCTAG ACAGACAGAC GGAGAGTGGA 
AACGCTGCTC TCCATTACTG CTGCACATAT GAGAAGCCAG AGTGTCTCAA ACTGCTGCTC 
AGGGGAAAAC CGTCTATTGA CCTGGTTAAT CAAAACGGGG AGACAGCATT GGATATCGCC 
AGACGACTGA GAAATGTACA GTGTGAAGAG CTACTGGTGG AGGCAGCAGC CGGGAGGTTT 
AATCCTCATG TGCATGTGGA GTATGAGTGG AATCTGCGGC TGGAGGAGAT TGATGAGAGT 
GACGATGACC TGGATGACAA GCCCAGTCCA GTGAAGAAGG AGCGTTCTCC TCGTCCTCAG 
AGCTTCTGTC ATTCGTCCAG CGTGTCTCCT CAGGAGAAGT TAACCCTGCC GGGGTATCTA 
GGACACAGGG ACAAGCAGAG ACTGTCCTAT GGAGCCTTTG CCAACCCCGT CTACAGCACC 
TCCACCGAAA CCCCTGCATC TCCAGTGTCA GAGGGACCCA CCATAGCCAG CAAGACCCCT 
GCAAAAGGAA GTGAGTCCAC ACCTCCTTCT GCAAATCGGA CATCCCCGGC CAACAAGTTT 
GAGGGAATCC AGCAGCAGCA AAGCACTACG TCTATGAACA CAAAAGCAAC ATTTGGCCCA 
CGAGTTCTTC CCAAACTACC TCAAAAAGTG GCACTACGAA AGATTGACAC AATCCACCTC 
CCATCAGTGG ACAAGTCTGG TCCTGATGTG CTTCAGAAAC CCCCACAGGC CCAGGATGCA 
CCTCCCACCA GAGCCTCAGA TACAATAACC AGACCCACTG AACCTCCACC TAAAATTCCA 
CAGGTCGCAG AACGATCCCA GCCTGTGGAT GTCCCGCAGA AACCGCACAT CTCAGACCTT 
CCTCCCAAAC CGCAACTATC AGATCTTCCC CCCAAACCCC AATTGTCGGA TTTACCACCA 
AAACCTCAGC TTTCTGACCT GCCCCCGAAG CCTCAGCTTA AGGATCTTCC CCCTAAGCCG 
CAGATCAGTG ATCTGCCATC CAAACCGGCC GTGTGTTCTG CATCTGAGGC CACACAAAGG 
CAGTCAACGC AGGAGGAAAC CAGTCCGAAG CCCCAGCTGA CGGAGACACA GTCATTCAGC 
CAGCAGGAGG AGCTCTCACC CCGACAGGCC AGCGAGGACA CCAATGGAGC GCCCGCAGGA 
GCCTTGGAAA TGCCAGTCCC AATGCCACGC AAAATTAACA CAGTAGCAAA GAACAAAGCG 
AAGCGTGTGA AAACCATCTA TGATTGCCAG GCAGACAATG ACGATGAGCT GACTTTTGTG 
GAGGGCGAGG TTATAATTGT CACAGGAGAG GAAGACCAGG AGTGGTGGAT CGGGCACATA 
GAGGGTCAGC CTGAAAGGAA AGGGGTCTTC CCAATGTCCT TCGTGCACAT TCTGTCAGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163534.1
CDS	292..3594
Translation	MRSSSSRLSSFSSRDSLWSRMPDQISVSEFLSETTEDYNSPTTSSFTTRLQSCRNTVNVLEEALDQDRTALQKVKKSVKAIYNSGQEHVQNEENYGQALDKFGSNFISRDNSDLGTAFIKFSGLIKELAALLKNLLQSLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRIFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKESRRDSQSKQSGYSMHQLQGNKEFGSEKKGYLFKKSDGIRKVWQRRKCSVKNGILTISHATSNRQPVRLNLLTCQVKPSGEDKKCFDLISHNRTYHFQAEDEQEFVIWISVLTNSKEEALNMAFRGEQSAGDDSLEDLTKAIIEDVLRIPGNEVCCDCGAPEPKWLSTNLGILTCIECSGIHREMGVHISRIQSMELDKLGTSELLLAKNVGNSSFNEILEGNLPSPSPKPAPSSDMTERKEYINAKYVEHRFARRTATTATARQGDLYEAVRTRDLMALIQLYADGVELMDPFPEAGQDPGETALHFAVRTSDQTSLHLVDFLVQNSGTLDRQTESGNAALHYCCTYEKPECLKLLLRGKPSIDLVNQNGETALDIARRLRNVQCEELLVEAAAGRFNPHVHVEYEWNLRLEEIDESDDDLDDKPSPVKKERSPRPQSFCHSSSVSPQEKLTLPGYLGHRDKQRLSYGAFANPVYSTSTETPASPVSEGPTIASKTPAKGSESTPPSANRTSPANKFEGIQQQQSTTSMNTKATFGPRVLPKLPQKVALRKIDTIHLPSVDKSGPDVLQKPPQAQDAPPTRASDTITRPTEPPPKIPQVAERSQPVDVPQKPHISDLPPKPQLSDLPPKPQLSDLPPKPQLSDLPPKPQLKDLPPKPQISDLPSKPAVCSASEATQRQSTQEETSPKPQLTETQSFSQQEELSPRQASEDTNGAPAGALEMPVPMPRKINTVAKNKAKRVKTIYDCQADNDDELTFVEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPMSFVHILSD

Target ORF information:

RefSeq Version	XM_005163477.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1a (asap1a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163477.3

ORF Insert Sequence:

ATGAGGTCCT CGTCCTCGCG TTTGTCAAGT TTTTCCTCCA GGGATTCATT ATGGAGTCGG 
ATGCCGGATC AGATCTCCGT GTCCGAGTTT CTCTCGGAGA CGACGGAGGA TTACAATTCC 
CCTACGACCT CGAGCTTCAC CACCCGCCTG CAGAGCTGCC GGAACACGGT CAATGTTCTG 
GAAGAGGCTT TGGATCAGGA CCGAACTGCT TTACAGAAGG TCAAGAAATC TGTCAAAGCA 
ATCTACAACT CGGGTCAAGA ACATGTGCAG AATGAAGAGA ATTATGGACA GGCACTGGAC 
AAGTTTGGCA GCAACTTCAT CAGCCGAGAT AACTCTGATC TGGGAACAGC CTTCATCAAG 
TTTTCTGGAC TTATCAAAGA GCTGGCTGCT CTCCTCAAGA ACCTGCTCCA GAGCCTCAGC 
CACAACGTCA TCTTCACCCT GGACTCTCTG CTCAAAGGAG ATCTAAAGGG AGTGAAGGGG 
GACCTTAAAA AGCCTTTTGA CAAGGCCTGG AAAGACTATG AAACCAAGTT CACAAAGATC 
GAGAAGGAGA AGAGAGAACA TGCCAAGCAG CACGGCATGA TCCGCACAGA GATCACCGGC 
GCAGAGATTG CAGAAGAGAT GGAGAAGGAG CGGAGGATCT TTCAGCTGCA GATGTGTGAG 
TACCTGATCA AAGTCAATGA GATTAAGACC AAGAAGGGAG TGGATCTCCT CCAGAATCTC 
ATCAAGTATT ATCATGCACA GTGCAATTTC TTCCAGGATG GCTTGAAAAC TGCTGACAAG 
TTGAAGCAGT ATATTGAAAA ATTAGCAGCT GATCTTTATA ATATAAAACA GACTCAGGAT 
GAGGAGAAAA AACAGCTCAC AGCTCTCAGA GACCTCATCA AATCTTCCTT ACAGCTGGAC 
CAGAAGGAGT CTAGGAGAGA TTCTCAGAGT AAGCAGAGCG GTTACAGCAT GCACCAGCTG 
CAGGGCAATA AGGAGTTTGG CAGTGAGAAG AAGGGCTATC TCTTTAAGAA GAGTGATGGG 
ATCCGTAAGG TGTGGCAGAG GAGGAAGTGC TCAGTGAAAA ATGGCATCCT CACCATCTCT 
CATGCCACAT CCAACAGGCA GCCGGTGAGA CTGAATCTGC TGACCTGCCA GGTTAAACCC 
AGTGGAGAGG ATAAGAAGTG CTTTGACCTC ATCTCTCATA ATCGAACATA TCATTTCCAG 
GCAGAGGACG AACAGGAGTT TGTGATATGG ATCTCGGTGC TGACTAATAG TAAGGAGGAG 
GCTCTGAACA TGGCATTTCG TGGGGAGCAG AGTGCTGGAG ATGACAGTTT GGAGGACTTG 
ACCAAAGCCA TCATCGAGGA CGTGCTGCGC ATTCCTGGAA ACGAAGTCTG CTGTGACTGT 
GGGGCTCCAG AGCCCAAATG GTTATCCACT AACCTCGGCA TCCTGACGTG CATCGAGTGT 
TCAGGAATCC ACAGGGAAAT GGGAGTCCAT ATTTCGCGCA TCCAATCCAT GGAGCTTGAC 
AAACTTGGAA CCTCTGAACT CTTGCTGGCT AAGAACGTGG GCAACAGTAG TTTCAACGAA 
ATATTAGAAG GGAATCTGCC GAGTCCTTCA CCAAAGCCAG CGCCATCAAG TGACATGACC 
GAGAGGAAGG AGTACATCAA TGCGAAGTAC GTGGAGCACA GGTTCGCTCG GCGAACGGCC 
ACTACAGCCA CAGCCAGACA GGGCGACTTG TACGAGGCGG TGAGAACGCG AGACTTGATG 
GCTCTCATTC AGCTCTATGC AGATGGAGTG GAGCTAATGG ATCCTTTCCC AGAAGCAGGA 
CAGGACCCGG GAGAGACAGC TCTGCACTTT GCTGTTCGGA CATCAGACCA GACTTCCCTG 
CACCTGGTGG ACTTTCTTGT CCAAAACAGT GGGACTCTAG ACAGACAGAC GGAGAGTGGA 
AACGCTGCTC TCCATTACTG CTGCACATAT GAGAAGCCAG AGTGTCTCAA ACTGCTGCTC 
AGGGGAAAAC CGTCTATTGA CCTGGTTAAT CAAAACGGGG AGACAGCATT GGATATCGCC 
AGACGACTGA GAAATGTACA GTGTGAAGAG CTACTGGTGG AGGCAGCAGC CGGGAGGTTT 
AATCCTCATG TGCATGTGGA GTATGAGTGG AATCTGCGGC TGGAGGAGAT TGATGAGAGT 
GACGATGACC TGGATGACAA GCCCAGTCCA GTGAAGAAGG AGCGTTCTCC TCGTCCTCAG 
AGCTTCTGTC ATTCGTCCAG CGTGTCTCCT CAGGAGAAGT TAACCCTGCC GGGGTATCTA 
GGACACAGGG ACAAGCAGAG ACTGTCCTAT GGAGCCTTTG CCAACCCCGT CTACAGCACC 
TCCACCGAAA CCCCTGCATC TCCAGTGTCA GAGGGACCCA CCATAGCCAG CAAGACCCCT 
GCAAAAGGAA GTGAGTCCAC ACCTCCTTCT GCAAATCGGA CATCCCCGGC CAACAAGTTT 
GAGGGAATCC AGCAGCAGCA AAGCACTACG TCTATGAACA CAAAAGCAAC ATTTGGCCCA 
CGAGTTCTTC CCAAACTACC TCAAAAAGTG GCACTACGAA AGATTGACAC AATCCACCTC 
CCATCAGTGG ACAAGTCTGG TCCTGATGTG CTTCAGAAAC CCCCACAGGC CCAGGATGCA 
CCTCCCACCA GAGCCTCAGA TACAATAACC AGACCCACTG AACCTCCACC TAAAATTCCA 
CAGGTCGCAG AACGATCCCA GCCTGTGGAT GTCCCGCAGA AACCGCACAT CTCAGACCTT 
CCTCCCAAAC CGCAACTATC AGATCTTCCC CCCAAACCCC AATTGTCGGA TTTACCACCA 
AAACCTCAGC TTTCTGACCT GCCCCCGAAG CCTCAGCTTA AGGATCTTCC CCCTAAGCCG 
CAGATCAGTG ATCTGCCATC CAAACCGGCC GTGTGTTCTG CATCTGAGGC CACACAAAGG 
CAGTCAACGC AGGAGGAAAC CAGTCCGAAG CCCCAGCTGA CGGAGACACA GTCATTCAGC 
CAGCAGGAGG AGCTCTCACC CCGACAGGCC AGCGAGGACA CCAATGGAGC GCCCGCAGGA 
GCCTTGGAAA TGCCAGTCCC AATGCCACGC AAAATTAACA CAGTAGCAAA GAACAAAGCG 
AAGCGTGTGA AAACCATCTA TGATTGCCAG GCAGACAATG ACGATGAGCT GACTTTTGTG 
GAGGGCGAGG TTATAATTGT CACAGGAGAG GAAGACCAGG AGTGGTGGAT CGGGCACATA 
GAGGGTCAGC CTGAAAGGAA AGGGGTCTTC CCAATGTCCT TCGTGCACAT TCTGTCAGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASAP1A ( XM_005163478.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005163535.1 Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1a (asap1a), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa07133
Clone ID Related Accession (Same CDS sequence)	XM_005163478.4 , XM_005163478.3
Accession Version	XM_005163478.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	arfGAP with SH3 domain, ankyrin repeat and PH domain 1a isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005163478.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTCCT CGTCCTCGCG TTTGTCAAGT TTTTCCTCCA GGGATTCATT ATGGAGTCGG 
ATGCCGGATC AGATCTCCGT GTCCGAGTTT CTCTCGGAGA CGACGGAGGA TTACAATTCC 
CCTACGACCT CGAGCTTCAC CACCCGCCTG CAGAGCTGCC GGAACACGGT CAATGTTCTG 
GAAGAGGCTT TGGATCAGGA CCGAACTGCT TTACAGAAGG TCAAGAAATC TGTCAAAGCA 
ATCTACAACT CGGGTCAAGA ACATGTGCAG AATGAAGAGA ATTATGGACA GGCACTGGAC 
AAGTTTGGCA GCAACTTCAT CAGCCGAGAT AACTCTGATC TGGGAACAGC CTTCATCAAG 
TTTTCTGGAC TTATCAAAGA GCTGGCTGCT CTCCTCAAGA ACCTGCTCCA GAGCCTCAGC 
CACAACGTCA TCTTCACCCT GGACTCTCTG CTCAAAGGAG ATCTAAAGGG AGTGAAGGGG 
GACCTTAAAA AGCCTTTTGA CAAGGCCTGG AAAGACTATG AAACCAAGTT CACAAAGATC 
GAGAAGGAGA AGAGAGAACA TGCCAAGCAG CACGGCATGA TCCGCACAGA GATCACCGGC 
GCAGAGATTG CAGAAGAGAT GGAGAAGGAG CGGAGGATCT TTCAGCTGCA GATGTGTGAG 
TACCTGATCA AAGTCAATGA GATTAAGACC AAGAAGGGAG TGGATCTCCT CCAGAATCTC 
ATCAAGTATT ATCATGCACA GTGCAATTTC TTCCAGGATG GCTTGAAAAC TGCTGACAAG 
TTGAAGCAGT ATATTGAAAA ATTAGCAGCT GATCTTTATA ATATAAAACA GACTCAGGAT 
GAGGAGAAAA AACAGCTCAC AGCTCTCAGA GACCTCATCA AATCTTCCTT ACAGCTGGAC 
CAGAAGGAGG ATTCTCAGAG TAAGCAGAGC GGTTACAGCA TGCACCAGCT GCAGGGCAAT 
AAGGAGTTTG GCAGTGAGAA GAAGGGCTAT CTCTTTAAGA AGAGTGATGG GATCCGTAAG 
GTGTGGCAGA GGAGGAAGTG CTCAGTGAAA AATGGCATCC TCACCATCTC TCATGCCACA 
TCCAACAGGC AGCCGGTGAG ACTGAATCTG CTGACCTGCC AGGTTAAACC CAGTGGAGAG 
GATAAGAAGT GCTTTGACCT CATCTCTCAT AATCGAACAT ATCATTTCCA GGCAGAGGAC 
GAACAGGAGT TTGTGATATG GATCTCGGTG CTGACTAATA GTAAGGAGGA GGCTCTGAAC 
ATGGCATTTC GTGGGGAGCA GAGTGCTGGA GATGACAGTT TGGAGGACTT GACCAAAGCC 
ATCATCGAGG ACGTGCTGCG CATTCCTGGA AACGAAGTCT GCTGTGACTG TGGGGCTCCA 
GAGCCCAAAT GGTTATCCAC TAACCTCGGC ATCCTGACGT GCATCGAGTG TTCAGGAATC 
CACAGGGAAA TGGGAGTCCA TATTTCGCGC ATCCAATCCA TGGAGCTTGA CAAACTTGGA 
ACCTCTGAAC TCTTGCTGGC TAAGAACGTG GGCAACAGTA GTTTCAACGA AATATTAGAA 
GGGAATCTGC CGAGTCCTTC ACCAAAGCCA GCGCCATCAA GTGACATGAC CGAGAGGAAG 
GAGTACATCA ATGCGAAGTA CGTGGAGCAC AGGTTCGCTC GGCGAACGGC CACTACAGCC 
ACAGCCAGAC AGGGCGACTT GTACGAGGCG GTGAGAACGC GAGACTTGAT GGCTCTCATT 
CAGCTCTATG CAGATGGAGT GGAGCTAATG GATCCTTTCC CAGAAGCAGG ACAGGACCCG 
GGAGAGACAG CTCTGCACTT TGCTGTTCGG ACATCAGACC AGACTTCCCT GCACCTGGTG 
GACTTTCTTG TCCAAAACAG TGGGACTCTA GACAGACAGA CGGAGAGTGG AAACGCTGCT 
CTCCATTACT GCTGCACATA TGAGAAGCCA GAGTGTCTCA AACTGCTGCT CAGGGGAAAA 
CCGTCTATTG ACCTGGTTAA TCAAAACGGG GAGACAGCAT TGGATATCGC CAGACGACTG 
AGAAATGTAC AGTGTGAAGA GCTACTGGTG GAGGCAGCAG CCGGGAGGTT TAATCCTCAT 
GTGCATGTGG AGTATGAGTG GAATCTGCGG CTGGAGGAGA TTGATGAGAG TGACGATGAC 
CTGGATGACA AGCCCAGTCC AGTGAAGAAG GAGCGTTCTC CTCGTCCTCA GAGCTTCTGT 
CATTCGTCCA GCGTGTCTCC TCAGGAGAAG TTAACCCTGC CGGGGTATCT AGGACACAGG 
GACAAGCAGA GACTGTCCTA TGGAGCCTTT GCCAACCCCG TCTACAGCAC CTCCACCGAA 
ACCCCTGCAT CTCCAGTGTC AGAGGGACCC ACCATAGCCA GCAAGACCCC TGCAAAAGGA 
AGTGAGTCCA CACCTCCTTC TGCAAATCGG ACATCCCCGG CCAACAAGTT TGAGGGAATC 
CAGCAGCAGC AAAGCACTAC GTCTATGAAC ACAAAAGCAA CATTTGGCCC ACGAGTTCTT 
CCCAAACTAC CTCAAAAAGT GGCACTACGA AAGATTGACA CAATCCACCT CCCATCAGTG 
GACAAGTCTG GTCCTGATGT GCTTCAGAAA CCCCCACAGG CCCAGGATGC ACCTCCCACC 
AGAGCCTCAG ATACAATAAC CAGACCCACT GAACCTCCAC CTAAAATTCC ACAGGTCGCA 
GAACGATCCC AGCCTGTGGA TGTCCCGCAG AAACCGCACA TCTCAGACCT TCCTCCCAAA 
CCGCAACTAT CAGATCTTCC CCCCAAACCC CAATTGTCGG ATTTACCACC AAAACCTCAG 
CTTTCTGACC TGCCCCCGAA GCCTCAGCTT AAGGATCTTC CCCCTAAGCC GCAGATCAGT 
GATCTGCCAT CCAAACCGGC CGTGTGTTCT GCATCTGAGG CCACACAAAG GCAGTCAACG 
CAGGAGGAAA CCAGTCCGAA GCCCCAGCTG ACGGAGACAC AGTCATTCAG CCAGCAGGAG 
GAGCTCTCAC CCCGACAGGC CAGCGAGGAC ACCAATGGAG CGCCCGCAGG AGCCTTGGAA 
ATGCCAGTCC CAATGCCACG CAAAATTAAC ACAGTAGCAA AGAACAAAGC GAAGCGTGTG 
AAAACCATCT ATGATTGCCA GGCAGACAAT GACGATGAGC TGACTTTTGT GGAGGGCGAG 
GTTATAATTG TCACAGGAGA GGAAGACCAG GAGTGGTGGA TCGGGCACAT AGAGGGTCAG 
CCTGAAAGGA AAGGGGTCTT CCCAATGTCC TTCGTGCACA TTCTGTCAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163535.1
CDS	276..3569
Translation	MRSSSSRLSSFSSRDSLWSRMPDQISVSEFLSETTEDYNSPTTSSFTTRLQSCRNTVNVLEEALDQDRTALQKVKKSVKAIYNSGQEHVQNEENYGQALDKFGSNFISRDNSDLGTAFIKFSGLIKELAALLKNLLQSLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRIFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKEDSQSKQSGYSMHQLQGNKEFGSEKKGYLFKKSDGIRKVWQRRKCSVKNGILTISHATSNRQPVRLNLLTCQVKPSGEDKKCFDLISHNRTYHFQAEDEQEFVIWISVLTNSKEEALNMAFRGEQSAGDDSLEDLTKAIIEDVLRIPGNEVCCDCGAPEPKWLSTNLGILTCIECSGIHREMGVHISRIQSMELDKLGTSELLLAKNVGNSSFNEILEGNLPSPSPKPAPSSDMTERKEYINAKYVEHRFARRTATTATARQGDLYEAVRTRDLMALIQLYADGVELMDPFPEAGQDPGETALHFAVRTSDQTSLHLVDFLVQNSGTLDRQTESGNAALHYCCTYEKPECLKLLLRGKPSIDLVNQNGETALDIARRLRNVQCEELLVEAAAGRFNPHVHVEYEWNLRLEEIDESDDDLDDKPSPVKKERSPRPQSFCHSSSVSPQEKLTLPGYLGHRDKQRLSYGAFANPVYSTSTETPASPVSEGPTIASKTPAKGSESTPPSANRTSPANKFEGIQQQQSTTSMNTKATFGPRVLPKLPQKVALRKIDTIHLPSVDKSGPDVLQKPPQAQDAPPTRASDTITRPTEPPPKIPQVAERSQPVDVPQKPHISDLPPKPQLSDLPPKPQLSDLPPKPQLSDLPPKPQLKDLPPKPQISDLPSKPAVCSASEATQRQSTQEETSPKPQLTETQSFSQQEELSPRQASEDTNGAPAGALEMPVPMPRKINTVAKNKAKRVKTIYDCQADNDDELTFVEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPMSFVHILSD

Target ORF information:

RefSeq Version	XM_005163478.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1a (asap1a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163478.4

ORF Insert Sequence:

ATGAGGTCCT CGTCCTCGCG TTTGTCAAGT TTTTCCTCCA GGGATTCATT ATGGAGTCGG 
ATGCCGGATC AGATCTCCGT GTCCGAGTTT CTCTCGGAGA CGACGGAGGA TTACAATTCC 
CCTACGACCT CGAGCTTCAC CACCCGCCTG CAGAGCTGCC GGAACACGGT CAATGTTCTG 
GAAGAGGCTT TGGATCAGGA CCGAACTGCT TTACAGAAGG TCAAGAAATC TGTCAAAGCA 
ATCTACAACT CGGGTCAAGA ACATGTGCAG AATGAAGAGA ATTATGGACA GGCACTGGAC 
AAGTTTGGCA GCAACTTCAT CAGCCGAGAT AACTCTGATC TGGGAACAGC CTTCATCAAG 
TTTTCTGGAC TTATCAAAGA GCTGGCTGCT CTCCTCAAGA ACCTGCTCCA GAGCCTCAGC 
CACAACGTCA TCTTCACCCT GGACTCTCTG CTCAAAGGAG ATCTAAAGGG AGTGAAGGGG 
GACCTTAAAA AGCCTTTTGA CAAGGCCTGG AAAGACTATG AAACCAAGTT CACAAAGATC 
GAGAAGGAGA AGAGAGAACA TGCCAAGCAG CACGGCATGA TCCGCACAGA GATCACCGGC 
GCAGAGATTG CAGAAGAGAT GGAGAAGGAG CGGAGGATCT TTCAGCTGCA GATGTGTGAG 
TACCTGATCA AAGTCAATGA GATTAAGACC AAGAAGGGAG TGGATCTCCT CCAGAATCTC 
ATCAAGTATT ATCATGCACA GTGCAATTTC TTCCAGGATG GCTTGAAAAC TGCTGACAAG 
TTGAAGCAGT ATATTGAAAA ATTAGCAGCT GATCTTTATA ATATAAAACA GACTCAGGAT 
GAGGAGAAAA AACAGCTCAC AGCTCTCAGA GACCTCATCA AATCTTCCTT ACAGCTGGAC 
CAGAAGGAGG ATTCTCAGAG TAAGCAGAGC GGTTACAGCA TGCACCAGCT GCAGGGCAAT 
AAGGAGTTTG GCAGTGAGAA GAAGGGCTAT CTCTTTAAGA AGAGTGATGG GATCCGTAAG 
GTGTGGCAGA GGAGGAAGTG CTCAGTGAAA AATGGCATCC TCACCATCTC TCATGCCACA 
TCCAACAGGC AGCCGGTGAG ACTGAATCTG CTGACCTGCC AGGTTAAACC CAGTGGAGAG 
GATAAGAAGT GCTTTGACCT CATCTCTCAT AATCGAACAT ATCATTTCCA GGCAGAGGAC 
GAACAGGAGT TTGTGATATG GATCTCGGTG CTGACTAATA GTAAGGAGGA GGCTCTGAAC 
ATGGCATTTC GTGGGGAGCA GAGTGCTGGA GATGACAGTT TGGAGGACTT GACCAAAGCC 
ATCATCGAGG ACGTGCTGCG CATTCCTGGA AACGAAGTCT GCTGTGACTG TGGGGCTCCA 
GAGCCCAAAT GGTTATCCAC TAACCTCGGC ATCCTGACGT GCATCGAGTG TTCAGGAATC 
CACAGGGAAA TGGGAGTCCA TATTTCGCGC ATCCAATCCA TGGAGCTTGA CAAACTTGGA 
ACCTCTGAAC TCTTGCTGGC TAAGAACGTG GGCAACAGTA GTTTCAACGA AATATTAGAA 
GGGAATCTGC CGAGTCCTTC ACCAAAGCCA GCGCCATCAA GTGACATGAC CGAGAGGAAG 
GAGTACATCA ATGCGAAGTA CGTGGAGCAC AGGTTCGCTC GGCGAACGGC CACTACAGCC 
ACAGCCAGAC AGGGCGACTT GTACGAGGCG GTGAGAACGC GAGACTTGAT GGCTCTCATT 
CAGCTCTATG CAGATGGAGT GGAGCTAATG GATCCTTTCC CAGAAGCAGG ACAGGACCCG 
GGAGAGACAG CTCTGCACTT TGCTGTTCGG ACATCAGACC AGACTTCCCT GCACCTGGTG 
GACTTTCTTG TCCAAAACAG TGGGACTCTA GACAGACAGA CGGAGAGTGG AAACGCTGCT 
CTCCATTACT GCTGCACATA TGAGAAGCCA GAGTGTCTCA AACTGCTGCT CAGGGGAAAA 
CCGTCTATTG ACCTGGTTAA TCAAAACGGG GAGACAGCAT TGGATATCGC CAGACGACTG 
AGAAATGTAC AGTGTGAAGA GCTACTGGTG GAGGCAGCAG CCGGGAGGTT TAATCCTCAT 
GTGCATGTGG AGTATGAGTG GAATCTGCGG CTGGAGGAGA TTGATGAGAG TGACGATGAC 
CTGGATGACA AGCCCAGTCC AGTGAAGAAG GAGCGTTCTC CTCGTCCTCA GAGCTTCTGT 
CATTCGTCCA GCGTGTCTCC TCAGGAGAAG TTAACCCTGC CGGGGTATCT AGGACACAGG 
GACAAGCAGA GACTGTCCTA TGGAGCCTTT GCCAACCCCG TCTACAGCAC CTCCACCGAA 
ACCCCTGCAT CTCCAGTGTC AGAGGGACCC ACCATAGCCA GCAAGACCCC TGCAAAAGGA 
AGTGAGTCCA CACCTCCTTC TGCAAATCGG ACATCCCCGG CCAACAAGTT TGAGGGAATC 
CAGCAGCAGC AAAGCACTAC GTCTATGAAC ACAAAAGCAA CATTTGGCCC ACGAGTTCTT 
CCCAAACTAC CTCAAAAAGT GGCACTACGA AAGATTGACA CAATCCACCT CCCATCAGTG 
GACAAGTCTG GTCCTGATGT GCTTCAGAAA CCCCCACAGG CCCAGGATGC ACCTCCCACC 
AGAGCCTCAG ATACAATAAC CAGACCCACT GAACCTCCAC CTAAAATTCC ACAGGTCGCA 
GAACGATCCC AGCCTGTGGA TGTCCCGCAG AAACCGCACA TCTCAGACCT TCCTCCCAAA 
CCGCAACTAT CAGATCTTCC CCCCAAACCC CAATTGTCGG ATTTACCACC AAAACCTCAG 
CTTTCTGACC TGCCCCCGAA GCCTCAGCTT AAGGATCTTC CCCCTAAGCC GCAGATCAGT 
GATCTGCCAT CCAAACCGGC CGTGTGTTCT GCATCTGAGG CCACACAAAG GCAGTCAACG 
CAGGAGGAAA CCAGTCCGAA GCCCCAGCTG ACGGAGACAC AGTCATTCAG CCAGCAGGAG 
GAGCTCTCAC CCCGACAGGC CAGCGAGGAC ACCAATGGAG CGCCCGCAGG AGCCTTGGAA 
ATGCCAGTCC CAATGCCACG CAAAATTAAC ACAGTAGCAA AGAACAAAGC GAAGCGTGTG 
AAAACCATCT ATGATTGCCA GGCAGACAAT GACGATGAGC TGACTTTTGT GGAGGGCGAG 
GTTATAATTG TCACAGGAGA GGAAGACCAG GAGTGGTGGA TCGGGCACAT AGAGGGTCAG 
CCTGAAAGGA AAGGGGTCTT CCCAATGTCC TTCGTGCACA TTCTGTCAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASAP1A ( XM_005163478.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005163535.1 Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1a (asap1a), transcript variant X5, mRNA.

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CloneID	ODa07133
Clone ID Related Accession (Same CDS sequence)	XM_005163478.4 , XM_005163478.3
Accession Version	XM_005163478.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	arfGAP with SH3 domain, ankyrin repeat and PH domain 1a isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005163478.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTCCT CGTCCTCGCG TTTGTCAAGT TTTTCCTCCA GGGATTCATT ATGGAGTCGG 
ATGCCGGATC AGATCTCCGT GTCCGAGTTT CTCTCGGAGA CGACGGAGGA TTACAATTCC 
CCTACGACCT CGAGCTTCAC CACCCGCCTG CAGAGCTGCC GGAACACGGT CAATGTTCTG 
GAAGAGGCTT TGGATCAGGA CCGAACTGCT TTACAGAAGG TCAAGAAATC TGTCAAAGCA 
ATCTACAACT CGGGTCAAGA ACATGTGCAG AATGAAGAGA ATTATGGACA GGCACTGGAC 
AAGTTTGGCA GCAACTTCAT CAGCCGAGAT AACTCTGATC TGGGAACAGC CTTCATCAAG 
TTTTCTGGAC TTATCAAAGA GCTGGCTGCT CTCCTCAAGA ACCTGCTCCA GAGCCTCAGC 
CACAACGTCA TCTTCACCCT GGACTCTCTG CTCAAAGGAG ATCTAAAGGG AGTGAAGGGG 
GACCTTAAAA AGCCTTTTGA CAAGGCCTGG AAAGACTATG AAACCAAGTT CACAAAGATC 
GAGAAGGAGA AGAGAGAACA TGCCAAGCAG CACGGCATGA TCCGCACAGA GATCACCGGC 
GCAGAGATTG CAGAAGAGAT GGAGAAGGAG CGGAGGATCT TTCAGCTGCA GATGTGTGAG 
TACCTGATCA AAGTCAATGA GATTAAGACC AAGAAGGGAG TGGATCTCCT CCAGAATCTC 
ATCAAGTATT ATCATGCACA GTGCAATTTC TTCCAGGATG GCTTGAAAAC TGCTGACAAG 
TTGAAGCAGT ATATTGAAAA ATTAGCAGCT GATCTTTATA ATATAAAACA GACTCAGGAT 
GAGGAGAAAA AACAGCTCAC AGCTCTCAGA GACCTCATCA AATCTTCCTT ACAGCTGGAC 
CAGAAGGAGG ATTCTCAGAG TAAGCAGAGC GGTTACAGCA TGCACCAGCT GCAGGGCAAT 
AAGGAGTTTG GCAGTGAGAA GAAGGGCTAT CTCTTTAAGA AGAGTGATGG GATCCGTAAG 
GTGTGGCAGA GGAGGAAGTG CTCAGTGAAA AATGGCATCC TCACCATCTC TCATGCCACA 
TCCAACAGGC AGCCGGTGAG ACTGAATCTG CTGACCTGCC AGGTTAAACC CAGTGGAGAG 
GATAAGAAGT GCTTTGACCT CATCTCTCAT AATCGAACAT ATCATTTCCA GGCAGAGGAC 
GAACAGGAGT TTGTGATATG GATCTCGGTG CTGACTAATA GTAAGGAGGA GGCTCTGAAC 
ATGGCATTTC GTGGGGAGCA GAGTGCTGGA GATGACAGTT TGGAGGACTT GACCAAAGCC 
ATCATCGAGG ACGTGCTGCG CATTCCTGGA AACGAAGTCT GCTGTGACTG TGGGGCTCCA 
GAGCCCAAAT GGTTATCCAC TAACCTCGGC ATCCTGACGT GCATCGAGTG TTCAGGAATC 
CACAGGGAAA TGGGAGTCCA TATTTCGCGC ATCCAATCCA TGGAGCTTGA CAAACTTGGA 
ACCTCTGAAC TCTTGCTGGC TAAGAACGTG GGCAACAGTA GTTTCAACGA AATATTAGAA 
GGGAATCTGC CGAGTCCTTC ACCAAAGCCA GCGCCATCAA GTGACATGAC CGAGAGGAAG 
GAGTACATCA ATGCGAAGTA CGTGGAGCAC AGGTTCGCTC GGCGAACGGC CACTACAGCC 
ACAGCCAGAC AGGGCGACTT GTACGAGGCG GTGAGAACGC GAGACTTGAT GGCTCTCATT 
CAGCTCTATG CAGATGGAGT GGAGCTAATG GATCCTTTCC CAGAAGCAGG ACAGGACCCG 
GGAGAGACAG CTCTGCACTT TGCTGTTCGG ACATCAGACC AGACTTCCCT GCACCTGGTG 
GACTTTCTTG TCCAAAACAG TGGGACTCTA GACAGACAGA CGGAGAGTGG AAACGCTGCT 
CTCCATTACT GCTGCACATA TGAGAAGCCA GAGTGTCTCA AACTGCTGCT CAGGGGAAAA 
CCGTCTATTG ACCTGGTTAA TCAAAACGGG GAGACAGCAT TGGATATCGC CAGACGACTG 
AGAAATGTAC AGTGTGAAGA GCTACTGGTG GAGGCAGCAG CCGGGAGGTT TAATCCTCAT 
GTGCATGTGG AGTATGAGTG GAATCTGCGG CTGGAGGAGA TTGATGAGAG TGACGATGAC 
CTGGATGACA AGCCCAGTCC AGTGAAGAAG GAGCGTTCTC CTCGTCCTCA GAGCTTCTGT 
CATTCGTCCA GCGTGTCTCC TCAGGAGAAG TTAACCCTGC CGGGGTATCT AGGACACAGG 
GACAAGCAGA GACTGTCCTA TGGAGCCTTT GCCAACCCCG TCTACAGCAC CTCCACCGAA 
ACCCCTGCAT CTCCAGTGTC AGAGGGACCC ACCATAGCCA GCAAGACCCC TGCAAAAGGA 
AGTGAGTCCA CACCTCCTTC TGCAAATCGG ACATCCCCGG CCAACAAGTT TGAGGGAATC 
CAGCAGCAGC AAAGCACTAC GTCTATGAAC ACAAAAGCAA CATTTGGCCC ACGAGTTCTT 
CCCAAACTAC CTCAAAAAGT GGCACTACGA AAGATTGACA CAATCCACCT CCCATCAGTG 
GACAAGTCTG GTCCTGATGT GCTTCAGAAA CCCCCACAGG CCCAGGATGC ACCTCCCACC 
AGAGCCTCAG ATACAATAAC CAGACCCACT GAACCTCCAC CTAAAATTCC ACAGGTCGCA 
GAACGATCCC AGCCTGTGGA TGTCCCGCAG AAACCGCACA TCTCAGACCT TCCTCCCAAA 
CCGCAACTAT CAGATCTTCC CCCCAAACCC CAATTGTCGG ATTTACCACC AAAACCTCAG 
CTTTCTGACC TGCCCCCGAA GCCTCAGCTT AAGGATCTTC CCCCTAAGCC GCAGATCAGT 
GATCTGCCAT CCAAACCGGC CGTGTGTTCT GCATCTGAGG CCACACAAAG GCAGTCAACG 
CAGGAGGAAA CCAGTCCGAA GCCCCAGCTG ACGGAGACAC AGTCATTCAG CCAGCAGGAG 
GAGCTCTCAC CCCGACAGGC CAGCGAGGAC ACCAATGGAG CGCCCGCAGG AGCCTTGGAA 
ATGCCAGTCC CAATGCCACG CAAAATTAAC ACAGTAGCAA AGAACAAAGC GAAGCGTGTG 
AAAACCATCT ATGATTGCCA GGCAGACAAT GACGATGAGC TGACTTTTGT GGAGGGCGAG 
GTTATAATTG TCACAGGAGA GGAAGACCAG GAGTGGTGGA TCGGGCACAT AGAGGGTCAG 
CCTGAAAGGA AAGGGGTCTT CCCAATGTCC TTCGTGCACA TTCTGTCAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163535.1
CDS	291..3584
Translation	MRSSSSRLSSFSSRDSLWSRMPDQISVSEFLSETTEDYNSPTTSSFTTRLQSCRNTVNVLEEALDQDRTALQKVKKSVKAIYNSGQEHVQNEENYGQALDKFGSNFISRDNSDLGTAFIKFSGLIKELAALLKNLLQSLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRIFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKEDSQSKQSGYSMHQLQGNKEFGSEKKGYLFKKSDGIRKVWQRRKCSVKNGILTISHATSNRQPVRLNLLTCQVKPSGEDKKCFDLISHNRTYHFQAEDEQEFVIWISVLTNSKEEALNMAFRGEQSAGDDSLEDLTKAIIEDVLRIPGNEVCCDCGAPEPKWLSTNLGILTCIECSGIHREMGVHISRIQSMELDKLGTSELLLAKNVGNSSFNEILEGNLPSPSPKPAPSSDMTERKEYINAKYVEHRFARRTATTATARQGDLYEAVRTRDLMALIQLYADGVELMDPFPEAGQDPGETALHFAVRTSDQTSLHLVDFLVQNSGTLDRQTESGNAALHYCCTYEKPECLKLLLRGKPSIDLVNQNGETALDIARRLRNVQCEELLVEAAAGRFNPHVHVEYEWNLRLEEIDESDDDLDDKPSPVKKERSPRPQSFCHSSSVSPQEKLTLPGYLGHRDKQRLSYGAFANPVYSTSTETPASPVSEGPTIASKTPAKGSESTPPSANRTSPANKFEGIQQQQSTTSMNTKATFGPRVLPKLPQKVALRKIDTIHLPSVDKSGPDVLQKPPQAQDAPPTRASDTITRPTEPPPKIPQVAERSQPVDVPQKPHISDLPPKPQLSDLPPKPQLSDLPPKPQLSDLPPKPQLKDLPPKPQISDLPSKPAVCSASEATQRQSTQEETSPKPQLTETQSFSQQEELSPRQASEDTNGAPAGALEMPVPMPRKINTVAKNKAKRVKTIYDCQADNDDELTFVEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPMSFVHILSD

Target ORF information:

RefSeq Version	XM_005163478.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 1a (asap1a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163478.3

ORF Insert Sequence:

ATGAGGTCCT CGTCCTCGCG TTTGTCAAGT TTTTCCTCCA GGGATTCATT ATGGAGTCGG 
ATGCCGGATC AGATCTCCGT GTCCGAGTTT CTCTCGGAGA CGACGGAGGA TTACAATTCC 
CCTACGACCT CGAGCTTCAC CACCCGCCTG CAGAGCTGCC GGAACACGGT CAATGTTCTG 
GAAGAGGCTT TGGATCAGGA CCGAACTGCT TTACAGAAGG TCAAGAAATC TGTCAAAGCA 
ATCTACAACT CGGGTCAAGA ACATGTGCAG AATGAAGAGA ATTATGGACA GGCACTGGAC 
AAGTTTGGCA GCAACTTCAT CAGCCGAGAT AACTCTGATC TGGGAACAGC CTTCATCAAG 
TTTTCTGGAC TTATCAAAGA GCTGGCTGCT CTCCTCAAGA ACCTGCTCCA GAGCCTCAGC 
CACAACGTCA TCTTCACCCT GGACTCTCTG CTCAAAGGAG ATCTAAAGGG AGTGAAGGGG 
GACCTTAAAA AGCCTTTTGA CAAGGCCTGG AAAGACTATG AAACCAAGTT CACAAAGATC 
GAGAAGGAGA AGAGAGAACA TGCCAAGCAG CACGGCATGA TCCGCACAGA GATCACCGGC 
GCAGAGATTG CAGAAGAGAT GGAGAAGGAG CGGAGGATCT TTCAGCTGCA GATGTGTGAG 
TACCTGATCA AAGTCAATGA GATTAAGACC AAGAAGGGAG TGGATCTCCT CCAGAATCTC 
ATCAAGTATT ATCATGCACA GTGCAATTTC TTCCAGGATG GCTTGAAAAC TGCTGACAAG 
TTGAAGCAGT ATATTGAAAA ATTAGCAGCT GATCTTTATA ATATAAAACA GACTCAGGAT 
GAGGAGAAAA AACAGCTCAC AGCTCTCAGA GACCTCATCA AATCTTCCTT ACAGCTGGAC 
CAGAAGGAGG ATTCTCAGAG TAAGCAGAGC GGTTACAGCA TGCACCAGCT GCAGGGCAAT 
AAGGAGTTTG GCAGTGAGAA GAAGGGCTAT CTCTTTAAGA AGAGTGATGG GATCCGTAAG 
GTGTGGCAGA GGAGGAAGTG CTCAGTGAAA AATGGCATCC TCACCATCTC TCATGCCACA 
TCCAACAGGC AGCCGGTGAG ACTGAATCTG CTGACCTGCC AGGTTAAACC CAGTGGAGAG 
GATAAGAAGT GCTTTGACCT CATCTCTCAT AATCGAACAT ATCATTTCCA GGCAGAGGAC 
GAACAGGAGT TTGTGATATG GATCTCGGTG CTGACTAATA GTAAGGAGGA GGCTCTGAAC 
ATGGCATTTC GTGGGGAGCA GAGTGCTGGA GATGACAGTT TGGAGGACTT GACCAAAGCC 
ATCATCGAGG ACGTGCTGCG CATTCCTGGA AACGAAGTCT GCTGTGACTG TGGGGCTCCA 
GAGCCCAAAT GGTTATCCAC TAACCTCGGC ATCCTGACGT GCATCGAGTG TTCAGGAATC 
CACAGGGAAA TGGGAGTCCA TATTTCGCGC ATCCAATCCA TGGAGCTTGA CAAACTTGGA 
ACCTCTGAAC TCTTGCTGGC TAAGAACGTG GGCAACAGTA GTTTCAACGA AATATTAGAA 
GGGAATCTGC CGAGTCCTTC ACCAAAGCCA GCGCCATCAA GTGACATGAC CGAGAGGAAG 
GAGTACATCA ATGCGAAGTA CGTGGAGCAC AGGTTCGCTC GGCGAACGGC CACTACAGCC 
ACAGCCAGAC AGGGCGACTT GTACGAGGCG GTGAGAACGC GAGACTTGAT GGCTCTCATT 
CAGCTCTATG CAGATGGAGT GGAGCTAATG GATCCTTTCC CAGAAGCAGG ACAGGACCCG 
GGAGAGACAG CTCTGCACTT TGCTGTTCGG ACATCAGACC AGACTTCCCT GCACCTGGTG 
GACTTTCTTG TCCAAAACAG TGGGACTCTA GACAGACAGA CGGAGAGTGG AAACGCTGCT 
CTCCATTACT GCTGCACATA TGAGAAGCCA GAGTGTCTCA AACTGCTGCT CAGGGGAAAA 
CCGTCTATTG ACCTGGTTAA TCAAAACGGG GAGACAGCAT TGGATATCGC CAGACGACTG 
AGAAATGTAC AGTGTGAAGA GCTACTGGTG GAGGCAGCAG CCGGGAGGTT TAATCCTCAT 
GTGCATGTGG AGTATGAGTG GAATCTGCGG CTGGAGGAGA TTGATGAGAG TGACGATGAC 
CTGGATGACA AGCCCAGTCC AGTGAAGAAG GAGCGTTCTC CTCGTCCTCA GAGCTTCTGT 
CATTCGTCCA GCGTGTCTCC TCAGGAGAAG TTAACCCTGC CGGGGTATCT AGGACACAGG 
GACAAGCAGA GACTGTCCTA TGGAGCCTTT GCCAACCCCG TCTACAGCAC CTCCACCGAA 
ACCCCTGCAT CTCCAGTGTC AGAGGGACCC ACCATAGCCA GCAAGACCCC TGCAAAAGGA 
AGTGAGTCCA CACCTCCTTC TGCAAATCGG ACATCCCCGG CCAACAAGTT TGAGGGAATC 
CAGCAGCAGC AAAGCACTAC GTCTATGAAC ACAAAAGCAA CATTTGGCCC ACGAGTTCTT 
CCCAAACTAC CTCAAAAAGT GGCACTACGA AAGATTGACA CAATCCACCT CCCATCAGTG 
GACAAGTCTG GTCCTGATGT GCTTCAGAAA CCCCCACAGG CCCAGGATGC ACCTCCCACC 
AGAGCCTCAG ATACAATAAC CAGACCCACT GAACCTCCAC CTAAAATTCC ACAGGTCGCA 
GAACGATCCC AGCCTGTGGA TGTCCCGCAG AAACCGCACA TCTCAGACCT TCCTCCCAAA 
CCGCAACTAT CAGATCTTCC CCCCAAACCC CAATTGTCGG ATTTACCACC AAAACCTCAG 
CTTTCTGACC TGCCCCCGAA GCCTCAGCTT AAGGATCTTC CCCCTAAGCC GCAGATCAGT 
GATCTGCCAT CCAAACCGGC CGTGTGTTCT GCATCTGAGG CCACACAAAG GCAGTCAACG 
CAGGAGGAAA CCAGTCCGAA GCCCCAGCTG ACGGAGACAC AGTCATTCAG CCAGCAGGAG 
GAGCTCTCAC CCCGACAGGC CAGCGAGGAC ACCAATGGAG CGCCCGCAGG AGCCTTGGAA 
ATGCCAGTCC CAATGCCACG CAAAATTAAC ACAGTAGCAA AGAACAAAGC GAAGCGTGTG 
AAAACCATCT ATGATTGCCA GGCAGACAAT GACGATGAGC TGACTTTTGT GGAGGGCGAG 
GTTATAATTG TCACAGGAGA GGAAGACCAG GAGTGGTGGA TCGGGCACAT AGAGGGTCAG 
CCTGAAAGGA AAGGGGTCTT CCCAATGTCC TTCGTGCACA TTCTGTCAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ASAP1A
Entrez Gene ID	100170829
Full Name	ArfGAP with SH3 domain, ankyrin repeat and PH domain 1a
Synonyms	si:ch211-160i2.3
General protein information	Preferred Names ArfGAP with SH3 domain, ankyrin repeat and PH domain 1a Names ArfGAP with SH3 domain, ankyrin repeat and PH domain 1a arfGAP with SH3 domain, ankyrin repeat and PH domain 1a
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ASAP1A cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

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