nid-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	nid-1
Entrez Gene ID	179770
Full Name	NIDogen (basement membrane protein)
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001269536.1	NP_001256465.1	NIDogen (basement membrane protein)
NM_001269538.1	NP_001256467.1	NIDogen (basement membrane protein)
NM_001269537.1	NP_001256466.1	NIDogen (basement membrane protein)
NM_001269536.2	NP_001256465.1	NIDogen (basement membrane protein)
NM_001269537.2	NP_001256466.1	NIDogen (basement membrane protein)
NM_001269538.2	NP_001256467.1	NIDogen (basement membrane protein)

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008193	XP_317275.4
Caenorhabditis elegans (roundworm)	nid-1	NP_001256466.1
Drosophila melanogaster (fruit fly)	Ndg	NP_995796.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

FBL-1C and LET-2 act downstream of MIG-17-dependent proteolysis to recruit NID-1 and that LET-2 also activates a NID-1-independent pathway, thereby inducing the remodeling of the BM required for directional control of leader cell migration.
Title: MIG-17/ADAMTS controls cell migration by recruiting nidogen to the basement membrane in C. elegans.

The following nid-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nid-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa346024	NM_001269538.2 Latest version!	Caenorhabditis elegans NIDogen (basement membrane protein) (nid-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCa345672	NM_001269536.2 Latest version!	Caenorhabditis elegans NIDogen (basement membrane protein) (nid-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OCa346023	NM_001269537.2 Latest version!	Caenorhabditis elegans NIDogen (basement membrane protein) (nid-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OCa345672	NM_001269536.1	Caenorhabditis elegans NIDogen (basement membrane protein) (nid-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCa346023	NM_001269537.1	Caenorhabditis elegans NIDogen (basement membrane protein) (nid-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCa346024	NM_001269538.1	Caenorhabditis elegans NIDogen (basement membrane protein) (nid-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nid-1 ( NM_001269538.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256467.1 Caenorhabditis elegans NIDogen (basement membrane protein) (nid-1), partial mRNA.

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CloneID	OCa346024
Clone ID Related Accession (Same CDS sequence)	NM_001269538.1 , NM_001269538.2
Accession Version	NM_001269538.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	NIDogen (basement membrane protein)
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGAAACACA AAAAAATGTT GATATTACTA TTTCTATATC TGTTGATAGA AACAACGACA 
GCAAACCCTG CATTCTACCC GTATGGACAA CCGGCTGGAG ATAATAGACT TGACTATTCT 
ATGGAACAAA GTGCAATTGC TCTTAAAATC CCACTGACAT ATATGGAACA GTCCGTTGAT 
GAGCTCTTTC TCTCACACAA TGGAATTGTC GGTCTTGGAA AACCGACGCC GGAGAAGATT 
GGAGGCCTAA TGACAATCTC TACACCTGCC ATAGCAGTTT TCTACGTGCC GGTTACAAGT 
GGAGTAATTG ATTATAGAGT TAGCTCTGAT GATCAGGGAC TGTTGACAAA ACTGACGCAA 
GATGTGAAAC AAGTTTTTGC GGATGCTGTT GAGTTCCATG GATTGCAAGC AGTAATCATC 
ACGTGGACAC AAATTGAGAA TGCTGAGAAA GATGGGCCTG CTAGTTTCCA GCTGGCAATT 
GTGTCAGACG GAATTTCAAC GTATGCAATT TTCCGTTATG AATCACTCCC CTGGTCATCT 
TCAATGGGAT ACTACGCACA GGCGGGATTC GTCCGCTCCA TTGGAAAAAT TCAGACAAAT 
GTGAATTCCG GAGGACCGGA TGTTAAGGAA CTTGTAAATT TGTCCAATAA TCAGTTCGGT 
AACTTTTTCA TCTTCCGAGT TTCGGGATCA GCAATTGAAG ATCCAAAGGA TGAAGGAGCT 
GATGATTATG ATTACAACAA TTACGATCAG GAATACGAGG GCGAACATCC AGCCGATTGC 
CCAAAAGACA AATTTGTTGA CAACTGCCCA GCCGAGTGTA GAACTGTAAG AGATGAACGA 
GGATGTGAAA GATGTATTTG TGCAGAGAGA CCACATGCCC CAAGAGCTCC TCAAGAGCCA 
AGACCAGAAC TGGAGAACAC TGTTGAAGAC GATGAAGATG ATGAACAAGT TGATGGAACA 
AGATATGAAG AGCCAGCTAG ACAGGCTCAG GAGCAACATC ACCAGCAACA CCATCAGCCG 
GATCAACCTC AGCAGCAGCC GCAACGCAGT CAGGAAGGAT CATTACCGAA AATGTCGTGC 
TCTCAAAGAG ATGACAAGAG CTGTCATGCA AACTCGGTTT GTCAAGATTT CGAAGGCGGA 
TTCTGCTGTA ACTGTGACAC GGGATTCTAT GGAAACGGAA AAGAGTGTCT TCCAAAAGGT 
GAACCACAAA GAATTTCTGG AAGCTTCGAG GGAGTCATCA ACAGAATTCC AATTGACAAG 
ACTGAACTTC ACACTTTTGC AACTTCGACT GATGGAAATG TTCACACAGC TGTTTCAAAG 
ATTCCATCGG ATCTTGGACA TCCACTTCGT TTCCTTTACT CAATTGGAGG AGTCATGGGA 
TGGCTATTTG CCGACGTTCA ATCCCCGAAT GTCTACAATG GATTCCAACT GACCGGTGGA 
CTTTTCAATA GAACCGTTGC ACTTCATATT GAACAAAACT ATTACGTAAC GATTAAGCAA 
GAGTTCTCCG GAAGAAACAT TCATGATTAC TTCAAATCTC ATTTATTTGT ATCGGGAACT 
CTTCCTGATA TTGCTCCAGG ATCTGAAGTT ATCTTCCCAG ATTATGAAGA AGAATACGTT 
AGAGAAAGAC GAGGATACTT AACATCAAAA GCAGCTTTCG ATGTAATCGT TAGAGATGGA 
GGAAATGTTC AAACATATCG AATGAGTGTT GATCAACAAA TTACTTTTGA AGAATGCCCA 
AATAAAGAGT TTGATCGTGA TCATTCAATG AAGCTTCATG TGAAGAGAAT TAATGTTGTC 
TATAACGATG ACGAAGGAGT CGTCCGATAT GGAGCAAAAA ACTTTGCAAC TCGATCAGTT 
GGGCCTGCTG TTTCAGCTCC TTCCGGTGGG CATTTCGATC GTCGCCAACA CGGACAATCC 
AACATGGCTT CAGAAAGACC AATTGAAATT CCAAGCCAAA GTGAATCAAT CAGTACTGAC 
TCAGTTTGTG CTCCAGGAAG ACATCAATGT ACTCTTCCAA ATATGAAGTG CCGTGTCGTT 
GATCCATCAT ACCGTTGTGA ATGTGAACCT GGATATCAAG CAGCTCATGA TGCATCCTCT 
CATATTGGAT GGATCTGTCA AGATTTGGAT GAATGTCAAC GAGGAGATCA TAATTGTGAC 
CAGCATGCCA AGTGTACTAA TCGACCAGGA TCTTTCTCAT GCCAATGTCT TCAAGGATAT 
CAAGGAGATG GAAGATCTTG TATTCGGGAG CATCAAGCAT CTCATCACGA ACATAATCAA 
CAGACTCCAC AAGAAATGGC CGGTGTCGGA GCGACAACTG AAGGATTCTG CACTGCACAT 
AACCAATGCC ATCAATGGGG AGAATGTGTA TTCACTTCTG AGCATCCAAC CGGAAGATGC 
AAGTGTAGGG GATGGTATGT TGGAGATGGA GTTAATCATT GTGGACCACC AGAGGAGAAC 
ATGCCAAAAC ACAATGCAAA TATTCCGCAG AGAGGAGGCC AAGCTTGTGG AAGTTATGTA 
TGCGATGTGA ATGCTGAGTG TATGCCAGAA CCAAGTGGAG GATCTGAATG TGTATGCAAG 
GCCGGATTCT CAGGAAATGG AGTAACTTGT GAAAGTCTGT TGGATGATCG TCATGCTCAT 
TCATCTCATA ATCGACATGA ACAACAACAA CAAACTGGAT CTCTTGGAAA AGTGTGCCGC 
TCTCACGATG AATGCTCTGA GCACGGAAGC TGCGCTTATC ACCACTCACT CGGTTATTAT 
CAATGCGCAT GTACCGAGCC ATATGTTGGC AATGGAGTCG AATGTACACT TCCAGGATCT 
TCTGCTTCAG CAGAATCCTC TACTAGGAGC GACAAGTTGC TAGTCGCCCG TGGAATGGCC 
ATATTTGAAC GCTCAACAAA TCCAGACGAG TACGGAAAAC AGCTGATTGT GATCCCTCAT 
CATATCCCAG TTGGCATTGA CTTTGATTGT AAAGAAGAAA AAATTGTGTG GAGTGACATG 
TCTGGTCACT CAATTCGCAC ATCTTCATTA AATGGAACTG AACATAAATC ATACTTCAAT 
AAAGAACTCA GCAGTCCTGA AGGAATTGCC GTTGACTGGT CTTCAAGAAA CGTGTACTAT 
GCTGACTCAA TGAATGATGA AATTGGAGTT GCTTCACTAA ATGGAAAATT CAAGAAATCT 
CTTGTAACTG AAGGTCTTGT CAATCCACGT TCAGTGGTTT TGGATCTATA CGGACGACAC 
TTGTACTACT CGGATTGGCA TCGTGAGAAT CCATACATCG GAAGAGTTGA CATGGATGGA 
AAGAACAATC GAGTATTCTT AAATGAAGAT GTCCATCTTC CAAATGGTCT CACTATTCTT 
CCAAACAGAC GCGAACTCTG TTGGGTGGAC GCTGGAAATC ACAGACTTTC ATGTATTCAG 
TACAATGGAG CTGGTAGAAG AACTGTGTTC AGTAGTCTTC AGTATCCGTT TGGTTTGACT 
CATGATGAAG AACAAAAATT CTATTGGACT GATTGGAAAG ACAACCGCAT TCATTCAGTT 
GGTGTCTATG GAGAAGGATA CCGTAGCTTC CAAATTTCCC TTGGTGGATC AGGAAAAGTA 
TTTGGAATCC TTGCCGTTCC AAAATCATGC GTCGGTCCAT CAACTCCATG TTCTGAAGAC 
AACGGTGGCT GCCAACATCT TTGCCTTCCA GGACAGAATG GTGCAGTTTG CGAATGTCCG 
GACAATGTCA AAGTGAAGGG ATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001256467.1
CDS	1..3747
Translation	MKHKKMLILLFLYLLIETTTANPAFYPYGQPAGDNRLDYSMEQSAIALKIPLTYMEQSVDELFLSHNGIVGLGKPTPEKIGGLMTISTPAIAVFYVPVTSGVIDYRVSSDDQGLLTKLTQDVKQVFADAVEFHGLQAVIITWTQIENAEKDGPASFQLAIVSDGISTYAIFRYESLPWSSSMGYYAQAGFVRSIGKIQTNVNSGGPDVKELVNLSNNQFGNFFIFRVSGSAIEDPKDEGADDYDYNNYDQEYEGEHPADCPKDKFVDNCPAECRTVRDERGCERCICAERPHAPRAPQEPRPELENTVEDDEDDEQVDGTRYEEPARQAQEQHHQQHHQPDQPQQQPQRSQEGSLPKMSCSQRDDKSCHANSVCQDFEGGFCCNCDTGFYGNGKECLPKGEPQRISGSFEGVINRIPIDKTELHTFATSTDGNVHTAVSKIPSDLGHPLRFLYSIGGVMGWLFADVQSPNVYNGFQLTGGLFNRTVALHIEQNYYVTIKQEFSGRNIHDYFKSHLFVSGTLPDIAPGSEVIFPDYEEEYVRERRGYLTSKAAFDVIVRDGGNVQTYRMSVDQQITFEECPNKEFDRDHSMKLHVKRINVVYNDDEGVVRYGAKNFATRSVGPAVSAPSGGHFDRRQHGQSNMASERPIEIPSQSESISTDSVCAPGRHQCTLPNMKCRVVDPSYRCECEPGYQAAHDASSHIGWICQDLDECQRGDHNCDQHAKCTNRPGSFSCQCLQGYQGDGRSCIREHQASHHEHNQQTPQEMAGVGATTEGFCTAHNQCHQWGECVFTSEHPTGRCKCRGWYVGDGVNHCGPPEENMPKHNANIPQRGGQACGSYVCDVNAECMPEPSGGSECVCKAGFSGNGVTCESLLDDRHAHSSHNRHEQQQQTGSLGKVCRSHDECSEHGSCAYHHSLGYYQCACTEPYVGNGVECTLPGSSASAESSTRSDKLLVARGMAIFERSTNPDEYGKQLIVIPHHIPVGIDFDCKEEKIVWSDMSGHSIRTSSLNGTEHKSYFNKELSSPEGIAVDWSSRNVYYADSMNDEIGVASLNGKFKKSLVTEGLVNPRSVVLDLYGRHLYYSDWHRENPYIGRVDMDGKNNRVFLNEDVHLPNGLTILPNRRELCWVDAGNHRLSCIQYNGAGRRTVFSSLQYPFGLTHDEEQKFYWTDWKDNRIHSVGVYGEGYRSFQISLGGSGKVFGILAVPKSCVGPSTPCSEDNGGCQHLCLPGQNGAVCECPDNVKVKGC

Target ORF information:

RefSeq Version	NM_001269538.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans NIDogen (basement membrane protein) (nid-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001269538.1

ORF Insert Sequence:

ATGAAACACA AAAAAATGTT GATATTACTA TTTCTATATC TGTTGATAGA AACAACGACA 
GCAAACCCTG CATTCTACCC GTATGGACAA CCGGCTGGAG ATAATAGACT TGACTATTCT 
ATGGAACAAA GTGCAATTGC TCTTAAAATC CCACTGACAT ATATGGAACA GTCCGTTGAT 
GAGCTCTTTC TCTCACACAA TGGAATTGTC GGTCTTGGAA AACCGACGCC GGAGAAGATT 
GGAGGCCTAA TGACAATCTC TACACCTGCC ATAGCAGTTT TCTACGTGCC GGTTACAAGT 
GGAGTAATTG ATTATAGAGT TAGCTCTGAT GATCAGGGAC TGTTGACAAA ACTGACGCAA 
GATGTGAAAC AAGTTTTTGC GGATGCTGTT GAGTTCCATG GATTGCAAGC AGTAATCATC 
ACGTGGACAC AAATTGAGAA TGCTGAGAAA GATGGGCCTG CTAGTTTCCA GCTGGCAATT 
GTGTCAGACG GAATTTCAAC GTATGCAATT TTCCGTTATG AATCACTCCC CTGGTCATCT 
TCAATGGGAT ACTACGCACA GGCGGGATTC GTCCGCTCCA TTGGAAAAAT TCAGACAAAT 
GTGAATTCCG GAGGACCGGA TGTTAAGGAA CTTGTAAATT TGTCCAATAA TCAGTTCGGT 
AACTTTTTCA TCTTCCGAGT TTCGGGATCA GCAATTGAAG ATCCAAAGGA TGAAGGAGCT 
GATGATTATG ATTACAACAA TTACGATCAG GAATACGAGG GCGAACATCC AGCCGATTGC 
CCAAAAGACA AATTTGTTGA CAACTGCCCA GCCGAGTGTA GAACTGTAAG AGATGAACGA 
GGATGTGAAA GATGTATTTG TGCAGAGAGA CCACATGCCC CAAGAGCTCC TCAAGAGCCA 
AGACCAGAAC TGGAGAACAC TGTTGAAGAC GATGAAGATG ATGAACAAGT TGATGGAACA 
AGATATGAAG AGCCAGCTAG ACAGGCTCAG GAGCAACATC ACCAGCAACA CCATCAGCCG 
GATCAACCTC AGCAGCAGCC GCAACGCAGT CAGGAAGGAT CATTACCGAA AATGTCGTGC 
TCTCAAAGAG ATGACAAGAG CTGTCATGCA AACTCGGTTT GTCAAGATTT CGAAGGCGGA 
TTCTGCTGTA ACTGTGACAC GGGATTCTAT GGAAACGGAA AAGAGTGTCT TCCAAAAGGT 
GAACCACAAA GAATTTCTGG AAGCTTCGAG GGAGTCATCA ACAGAATTCC AATTGACAAG 
ACTGAACTTC ACACTTTTGC AACTTCGACT GATGGAAATG TTCACACAGC TGTTTCAAAG 
ATTCCATCGG ATCTTGGACA TCCACTTCGT TTCCTTTACT CAATTGGAGG AGTCATGGGA 
TGGCTATTTG CCGACGTTCA ATCCCCGAAT GTCTACAATG GATTCCAACT GACCGGTGGA 
CTTTTCAATA GAACCGTTGC ACTTCATATT GAACAAAACT ATTACGTAAC GATTAAGCAA 
GAGTTCTCCG GAAGAAACAT TCATGATTAC TTCAAATCTC ATTTATTTGT ATCGGGAACT 
CTTCCTGATA TTGCTCCAGG ATCTGAAGTT ATCTTCCCAG ATTATGAAGA AGAATACGTT 
AGAGAAAGAC GAGGATACTT AACATCAAAA GCAGCTTTCG ATGTAATCGT TAGAGATGGA 
GGAAATGTTC AAACATATCG AATGAGTGTT GATCAACAAA TTACTTTTGA AGAATGCCCA 
AATAAAGAGT TTGATCGTGA TCATTCAATG AAGCTTCATG TGAAGAGAAT TAATGTTGTC 
TATAACGATG ACGAAGGAGT CGTCCGATAT GGAGCAAAAA ACTTTGCAAC TCGATCAGTT 
GGGCCTGCTG TTTCAGCTCC TTCCGGTGGG CATTTCGATC GTCGCCAACA CGGACAATCC 
AACATGGCTT CAGAAAGACC AATTGAAATT CCAAGCCAAA GTGAATCAAT CAGTACTGAC 
TCAGTTTGTG CTCCAGGAAG ACATCAATGT ACTCTTCCAA ATATGAAGTG CCGTGTCGTT 
GATCCATCAT ACCGTTGTGA ATGTGAACCT GGATATCAAG CAGCTCATGA TGCATCCTCT 
CATATTGGAT GGATCTGTCA AGATTTGGAT GAATGTCAAC GAGGAGATCA TAATTGTGAC 
CAGCATGCCA AGTGTACTAA TCGACCAGGA TCTTTCTCAT GCCAATGTCT TCAAGGATAT 
CAAGGAGATG GAAGATCTTG TATTCGGGAG CATCAAGCAT CTCATCACGA ACATAATCAA 
CAGACTCCAC AAGAAATGGC CGGTGTCGGA GCGACAACTG AAGGATTCTG CACTGCACAT 
AACCAATGCC ATCAATGGGG AGAATGTGTA TTCACTTCTG AGCATCCAAC CGGAAGATGC 
AAGTGTAGGG GATGGTATGT TGGAGATGGA GTTAATCATT GTGGACCACC AGAGGAGAAC 
ATGCCAAAAC ACAATGCAAA TATTCCGCAG AGAGGAGGCC AAGCTTGTGG AAGTTATGTA 
TGCGATGTGA ATGCTGAGTG TATGCCAGAA CCAAGTGGAG GATCTGAATG TGTATGCAAG 
GCCGGATTCT CAGGAAATGG AGTAACTTGT GAAAGTCTGT TGGATGATCG TCATGCTCAT 
TCATCTCATA ATCGACATGA ACAACAACAA CAAACTGGAT CTCTTGGAAA AGTGTGCCGC 
TCTCACGATG AATGCTCTGA GCACGGAAGC TGCGCTTATC ACCACTCACT CGGTTATTAT 
CAATGCGCAT GTACCGAGCC ATATGTTGGC AATGGAGTCG AATGTACACT TCCAGGATCT 
TCTGCTTCAG CAGAATCCTC TACTAGGAGC GACAAGTTGC TAGTCGCCCG TGGAATGGCC 
ATATTTGAAC GCTCAACAAA TCCAGACGAG TACGGAAAAC AGCTGATTGT GATCCCTCAT 
CATATCCCAG TTGGCATTGA CTTTGATTGT AAAGAAGAAA AAATTGTGTG GAGTGACATG 
TCTGGTCACT CAATTCGCAC ATCTTCATTA AATGGAACTG AACATAAATC ATACTTCAAT 
AAAGAACTCA GCAGTCCTGA AGGAATTGCC GTTGACTGGT CTTCAAGAAA CGTGTACTAT 
GCTGACTCAA TGAATGATGA AATTGGAGTT GCTTCACTAA ATGGAAAATT CAAGAAATCT 
CTTGTAACTG AAGGTCTTGT CAATCCACGT TCAGTGGTTT TGGATCTATA CGGACGACAC 
TTGTACTACT CGGATTGGCA TCGTGAGAAT CCATACATCG GAAGAGTTGA CATGGATGGA 
AAGAACAATC GAGTATTCTT AAATGAAGAT GTCCATCTTC CAAATGGTCT CACTATTCTT 
CCAAACAGAC GCGAACTCTG TTGGGTGGAC GCTGGAAATC ACAGACTTTC ATGTATTCAG 
TACAATGGAG CTGGTAGAAG AACTGTGTTC AGTAGTCTTC AGTATCCGTT TGGTTTGACT 
CATGATGAAG AACAAAAATT CTATTGGACT GATTGGAAAG ACAACCGCAT TCATTCAGTT 
GGTGTCTATG GAGAAGGATA CCGTAGCTTC CAAATTTCCC TTGGTGGATC AGGAAAAGTA 
TTTGGAATCC TTGCCGTTCC AAAATCATGC GTCGGTCCAT CAACTCCATG TTCTGAAGAC 
AACGGTGGCT GCCAACATCT TTGCCTTCCA GGACAGAATG GTGCAGTTTG CGAATGTCCG 
GACAATGTCA AAGTGAAGGG ATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nid-1 ( NM_001269538.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256467.1 Caenorhabditis elegans NIDogen (basement membrane protein) (nid-1), mRNA.

Price & Availability↑

CloneID	OCa346024
Clone ID Related Accession (Same CDS sequence)	NM_001269538.1 , NM_001269538.2
Accession Version	NM_001269538.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	NIDogen (basement membrane protein)
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001269538.1.

ATGAAACACA AAAAAATGTT GATATTACTA TTTCTATATC TGTTGATAGA AACAACGACA 
GCAAACCCTG CATTCTACCC GTATGGACAA CCGGCTGGAG ATAATAGACT TGACTATTCT 
ATGGAACAAA GTGCAATTGC TCTTAAAATC CCACTGACAT ATATGGAACA GTCCGTTGAT 
GAGCTCTTTC TCTCACACAA TGGAATTGTC GGTCTTGGAA AACCGACGCC GGAGAAGATT 
GGAGGCCTAA TGACAATCTC TACACCTGCC ATAGCAGTTT TCTACGTGCC GGTTACAAGT 
GGAGTAATTG ATTATAGAGT TAGCTCTGAT GATCAGGGAC TGTTGACAAA ACTGACGCAA 
GATGTGAAAC AAGTTTTTGC GGATGCTGTT GAGTTCCATG GATTGCAAGC AGTAATCATC 
ACGTGGACAC AAATTGAGAA TGCTGAGAAA GATGGGCCTG CTAGTTTCCA GCTGGCAATT 
GTGTCAGACG GAATTTCAAC GTATGCAATT TTCCGTTATG AATCACTCCC CTGGTCATCT 
TCAATGGGAT ACTACGCACA GGCGGGATTC GTCCGCTCCA TTGGAAAAAT TCAGACAAAT 
GTGAATTCCG GAGGACCGGA TGTTAAGGAA CTTGTAAATT TGTCCAATAA TCAGTTCGGT 
AACTTTTTCA TCTTCCGAGT TTCGGGATCA GCAATTGAAG ATCCAAAGGA TGAAGGAGCT 
GATGATTATG ATTACAACAA TTACGATCAG GAATACGAGG GCGAACATCC AGCCGATTGC 
CCAAAAGACA AATTTGTTGA CAACTGCCCA GCCGAGTGTA GAACTGTAAG AGATGAACGA 
GGATGTGAAA GATGTATTTG TGCAGAGAGA CCACATGCCC CAAGAGCTCC TCAAGAGCCA 
AGACCAGAAC TGGAGAACAC TGTTGAAGAC GATGAAGATG ATGAACAAGT TGATGGAACA 
AGATATGAAG AGCCAGCTAG ACAGGCTCAG GAGCAACATC ACCAGCAACA CCATCAGCCG 
GATCAACCTC AGCAGCAGCC GCAACGCAGT CAGGAAGGAT CATTACCGAA AATGTCGTGC 
TCTCAAAGAG ATGACAAGAG CTGTCATGCA AACTCGGTTT GTCAAGATTT CGAAGGCGGA 
TTCTGCTGTA ACTGTGACAC GGGATTCTAT GGAAACGGAA AAGAGTGTCT TCCAAAAGGT 
GAACCACAAA GAATTTCTGG AAGCTTCGAG GGAGTCATCA ACAGAATTCC AATTGACAAG 
ACTGAACTTC ACACTTTTGC AACTTCGACT GATGGAAATG TTCACACAGC TGTTTCAAAG 
ATTCCATCGG ATCTTGGACA TCCACTTCGT TTCCTTTACT CAATTGGAGG AGTCATGGGA 
TGGCTATTTG CCGACGTTCA ATCCCCGAAT GTCTACAATG GATTCCAACT GACCGGTGGA 
CTTTTCAATA GAACCGTTGC ACTTCATATT GAACAAAACT ATTACGTAAC GATTAAGCAA 
GAGTTCTCCG GAAGAAACAT TCATGATTAC TTCAAATCTC ATTTATTTGT ATCGGGAACT 
CTTCCTGATA TTGCTCCAGG ATCTGAAGTT ATCTTCCCAG ATTATGAAGA AGAATACGTT 
AGAGAAAGAC GAGGATACTT AACATCAAAA GCAGCTTTCG ATGTAATCGT TAGAGATGGA 
GGAAATGTTC AAACATATCG AATGAGTGTT GATCAACAAA TTACTTTTGA AGAATGCCCA 
AATAAAGAGT TTGATCGTGA TCATTCAATG AAGCTTCATG TGAAGAGAAT TAATGTTGTC 
TATAACGATG ACGAAGGAGT CGTCCGATAT GGAGCAAAAA ACTTTGCAAC TCGATCAGTT 
GGGCCTGCTG TTTCAGCTCC TTCCGGTGGG CATTTCGATC GTCGCCAACA CGGACAATCC 
AACATGGCTT CAGAAAGACC AATTGAAATT CCAAGCCAAA GTGAATCAAT CAGTACTGAC 
TCAGTTTGTG CTCCAGGAAG ACATCAATGT ACTCTTCCAA ATATGAAGTG CCGTGTCGTT 
GATCCATCAT ACCGTTGTGA ATGTGAACCT GGATATCAAG CAGCTCATGA TGCATCCTCT 
CATATTGGAT GGATCTGTCA AGATTTGGAT GAATGTCAAC GAGGAGATCA TAATTGTGAC 
CAGCATGCCA AGTGTACTAA TCGACCAGGA TCTTTCTCAT GCCAATGTCT TCAAGGATAT 
CAAGGAGATG GAAGATCTTG TATTCGGGAG CATCAAGCAT CTCATCACGA ACATAATCAA 
CAGACTCCAC AAGAAATGGC CGGTGTCGGA GCGACAACTG AAGGATTCTG CACTGCACAT 
AACCAATGCC ATCAATGGGG AGAATGTGTA TTCACTTCTG AGCATCCAAC CGGAAGATGC 
AAGTGTAGGG GATGGTATGT TGGAGATGGA GTTAATCATT GTGGACCACC AGAGGAGAAC 
ATGCCAAAAC ACAATGCAAA TATTCCGCAG AGAGGAGGCC AAGCTTGTGG AAGTTATGTA 
TGCGATGTGA ATGCTGAGTG TATGCCAGAA CCAAGTGGAG GATCTGAATG TGTATGCAAG 
GCCGGATTCT CAGGAAATGG AGTAACTTGT GAAAGTCTGT TGGATGATCG TCATGCTCAT 
TCATCTCATA ATCGACATGA ACAACAACAA CAAACTGGAT CTCTTGGAAA AGTGTGCCGC 
TCTCACGATG AATGCTCTGA GCACGGAAGC TGCGCTTATC ACCACTCACT CGGTTATTAT 
CAATGCGCAT GTACCGAGCC ATATGTTGGC AATGGAGTCG AATGTACACT TCCAGGATCT 
TCTGCTTCAG CAGAATCCTC TACTAGGAGC GACAAGTTGC TAGTCGCCCG TGGAATGGCC 
ATATTTGAAC GCTCAACAAA TCCAGACGAG TACGGAAAAC AGCTGATTGT GATCCCTCAT 
CATATCCCAG TTGGCATTGA CTTTGATTGT AAAGAAGAAA AAATTGTGTG GAGTGACATG 
TCTGGTCACT CAATTCGCAC ATCTTCATTA AATGGAACTG AACATAAATC ATACTTCAAT 
AAAGAACTCA GCAGTCCTGA AGGAATTGCC GTTGACTGGT CTTCAAGAAA CGTGTACTAT 
GCTGACTCAA TGAATGATGA AATTGGAGTT GCTTCACTAA ATGGAAAATT CAAGAAATCT 
CTTGTAACTG AAGGTCTTGT CAATCCACGT TCAGTGGTTT TGGATCTATA CGGACGACAC 
TTGTACTACT CGGATTGGCA TCGTGAGAAT CCATACATCG GAAGAGTTGA CATGGATGGA 
AAGAACAATC GAGTATTCTT AAATGAAGAT GTCCATCTTC CAAATGGTCT CACTATTCTT 
CCAAACAGAC GCGAACTCTG TTGGGTGGAC GCTGGAAATC ACAGACTTTC ATGTATTCAG 
TACAATGGAG CTGGTAGAAG AACTGTGTTC AGTAGTCTTC AGTATCCGTT TGGTTTGACT 
CATGATGAAG AACAAAAATT CTATTGGACT GATTGGAAAG ACAACCGCAT TCATTCAGTT 
GGTGTCTATG GAGAAGGATA CCGTAGCTTC CAAATTTCCC TTGGTGGATC AGGAAAAGTA 
TTTGGAATCC TTGCCGTTCC AAAATCATGC GTCGGTCCAT CAACTCCATG TTCTGAAGAC 
AACGGTGGCT GCCAACATCT TTGCCTTCCA GGACAGAATG GTGCAGTTTG CGAATGTCCG 
GACAATGTCA AAGTGAAGGG ATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001256467.1
CDS	6..3752
Translation	MKHKKMLILLFLYLLIETTTANPAFYPYGQPAGDNRLDYSMEQSAIALKIPLTYMEQSVDELFLSHNGIVGLGKPTPEKIGGLMTISTPAIAVFYVPVTSGVIDYRVSSDDQGLLTKLTQDVKQVFADAVEFHGLQAVIITWTQIENAEKDGPASFQLAIVSDGISTYAIFRYESLPWSSSMGYYAQAGFVRSIGKIQTNVNSGGPDVKELVNLSNNQFGNFFIFRVSGSAIEDPKDEGADDYDYNNYDQEYEGEHPADCPKDKFVDNCPAECRTVRDERGCERCICAERPHAPRAPQEPRPELENTVEDDEDDEQVDGTRYEEPARQAQEQHHQQHHQPDQPQQQPQRSQEGSLPKMSCSQRDDKSCHANSVCQDFEGGFCCNCDTGFYGNGKECLPKGEPQRISGSFEGVINRIPIDKTELHTFATSTDGNVHTAVSKIPSDLGHPLRFLYSIGGVMGWLFADVQSPNVYNGFQLTGGLFNRTVALHIEQNYYVTIKQEFSGRNIHDYFKSHLFVSGTLPDIAPGSEVIFPDYEEEYVRERRGYLTSKAAFDVIVRDGGNVQTYRMSVDQQITFEECPNKEFDRDHSMKLHVKRINVVYNDDEGVVRYGAKNFATRSVGPAVSAPSGGHFDRRQHGQSNMASERPIEIPSQSESISTDSVCAPGRHQCTLPNMKCRVVDPSYRCECEPGYQAAHDASSHIGWICQDLDECQRGDHNCDQHAKCTNRPGSFSCQCLQGYQGDGRSCIREHQASHHEHNQQTPQEMAGVGATTEGFCTAHNQCHQWGECVFTSEHPTGRCKCRGWYVGDGVNHCGPPEENMPKHNANIPQRGGQACGSYVCDVNAECMPEPSGGSECVCKAGFSGNGVTCESLLDDRHAHSSHNRHEQQQQTGSLGKVCRSHDECSEHGSCAYHHSLGYYQCACTEPYVGNGVECTLPGSSASAESSTRSDKLLVARGMAIFERSTNPDEYGKQLIVIPHHIPVGIDFDCKEEKIVWSDMSGHSIRTSSLNGTEHKSYFNKELSSPEGIAVDWSSRNVYYADSMNDEIGVASLNGKFKKSLVTEGLVNPRSVVLDLYGRHLYYSDWHRENPYIGRVDMDGKNNRVFLNEDVHLPNGLTILPNRRELCWVDAGNHRLSCIQYNGAGRRTVFSSLQYPFGLTHDEEQKFYWTDWKDNRIHSVGVYGEGYRSFQISLGGSGKVFGILAVPKSCVGPSTPCSEDNGGCQHLCLPGQNGAVCECPDNVKVKGC

Target ORF information:

RefSeq Version	NM_001269538.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans NIDogen (basement membrane protein) (nid-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001269538.2

ORF Insert Sequence:

ATGAAACACA AAAAAATGTT GATATTACTA TTTCTATATC TGTTGATAGA AACAACGACA 
GCAAACCCTG CATTCTACCC GTATGGACAA CCGGCTGGAG ATAATAGACT TGACTATTCT 
ATGGAACAAA GTGCAATTGC TCTTAAAATC CCACTGACAT ATATGGAACA GTCCGTTGAT 
GAGCTCTTTC TCTCACACAA TGGAATTGTC GGTCTTGGAA AACCGACGCC GGAGAAGATT 
GGAGGCCTAA TGACAATCTC TACACCTGCC ATAGCAGTTT TCTACGTGCC GGTTACAAGT 
GGAGTAATTG ATTATAGAGT TAGCTCTGAT GATCAGGGAC TGTTGACAAA ACTGACGCAA 
GATGTGAAAC AAGTTTTTGC GGATGCTGTT GAGTTCCATG GATTGCAAGC AGTAATCATC 
ACGTGGACAC AAATTGAGAA TGCTGAGAAA GATGGGCCTG CTAGTTTCCA GCTGGCAATT 
GTGTCAGACG GAATTTCAAC GTATGCAATT TTCCGTTATG AATCACTCCC CTGGTCATCT 
TCAATGGGAT ACTACGCACA GGCGGGATTC GTCCGCTCCA TTGGAAAAAT TCAGACAAAT 
GTGAATTCCG GAGGACCGGA TGTTAAGGAA CTTGTAAATT TGTCCAATAA TCAGTTCGGT 
AACTTTTTCA TCTTCCGAGT TTCGGGATCA GCAATTGAAG ATCCAAAGGA TGAAGGAGCT 
GATGATTATG ATTACAACAA TTACGATCAG GAATACGAGG GCGAACATCC AGCCGATTGC 
CCAAAAGACA AATTTGTTGA CAACTGCCCA GCCGAGTGTA GAACTGTAAG AGATGAACGA 
GGATGTGAAA GATGTATTTG TGCAGAGAGA CCACATGCCC CAAGAGCTCC TCAAGAGCCA 
AGACCAGAAC TGGAGAACAC TGTTGAAGAC GATGAAGATG ATGAACAAGT TGATGGAACA 
AGATATGAAG AGCCAGCTAG ACAGGCTCAG GAGCAACATC ACCAGCAACA CCATCAGCCG 
GATCAACCTC AGCAGCAGCC GCAACGCAGT CAGGAAGGAT CATTACCGAA AATGTCGTGC 
TCTCAAAGAG ATGACAAGAG CTGTCATGCA AACTCGGTTT GTCAAGATTT CGAAGGCGGA 
TTCTGCTGTA ACTGTGACAC GGGATTCTAT GGAAACGGAA AAGAGTGTCT TCCAAAAGGT 
GAACCACAAA GAATTTCTGG AAGCTTCGAG GGAGTCATCA ACAGAATTCC AATTGACAAG 
ACTGAACTTC ACACTTTTGC AACTTCGACT GATGGAAATG TTCACACAGC TGTTTCAAAG 
ATTCCATCGG ATCTTGGACA TCCACTTCGT TTCCTTTACT CAATTGGAGG AGTCATGGGA 
TGGCTATTTG CCGACGTTCA ATCCCCGAAT GTCTACAATG GATTCCAACT GACCGGTGGA 
CTTTTCAATA GAACCGTTGC ACTTCATATT GAACAAAACT ATTACGTAAC GATTAAGCAA 
GAGTTCTCCG GAAGAAACAT TCATGATTAC TTCAAATCTC ATTTATTTGT ATCGGGAACT 
CTTCCTGATA TTGCTCCAGG ATCTGAAGTT ATCTTCCCAG ATTATGAAGA AGAATACGTT 
AGAGAAAGAC GAGGATACTT AACATCAAAA GCAGCTTTCG ATGTAATCGT TAGAGATGGA 
GGAAATGTTC AAACATATCG AATGAGTGTT GATCAACAAA TTACTTTTGA AGAATGCCCA 
AATAAAGAGT TTGATCGTGA TCATTCAATG AAGCTTCATG TGAAGAGAAT TAATGTTGTC 
TATAACGATG ACGAAGGAGT CGTCCGATAT GGAGCAAAAA ACTTTGCAAC TCGATCAGTT 
GGGCCTGCTG TTTCAGCTCC TTCCGGTGGG CATTTCGATC GTCGCCAACA CGGACAATCC 
AACATGGCTT CAGAAAGACC AATTGAAATT CCAAGCCAAA GTGAATCAAT CAGTACTGAC 
TCAGTTTGTG CTCCAGGAAG ACATCAATGT ACTCTTCCAA ATATGAAGTG CCGTGTCGTT 
GATCCATCAT ACCGTTGTGA ATGTGAACCT GGATATCAAG CAGCTCATGA TGCATCCTCT 
CATATTGGAT GGATCTGTCA AGATTTGGAT GAATGTCAAC GAGGAGATCA TAATTGTGAC 
CAGCATGCCA AGTGTACTAA TCGACCAGGA TCTTTCTCAT GCCAATGTCT TCAAGGATAT 
CAAGGAGATG GAAGATCTTG TATTCGGGAG CATCAAGCAT CTCATCACGA ACATAATCAA 
CAGACTCCAC AAGAAATGGC CGGTGTCGGA GCGACAACTG AAGGATTCTG CACTGCACAT 
AACCAATGCC ATCAATGGGG AGAATGTGTA TTCACTTCTG AGCATCCAAC CGGAAGATGC 
AAGTGTAGGG GATGGTATGT TGGAGATGGA GTTAATCATT GTGGACCACC AGAGGAGAAC 
ATGCCAAAAC ACAATGCAAA TATTCCGCAG AGAGGAGGCC AAGCTTGTGG AAGTTATGTA 
TGCGATGTGA ATGCTGAGTG TATGCCAGAA CCAAGTGGAG GATCTGAATG TGTATGCAAG 
GCCGGATTCT CAGGAAATGG AGTAACTTGT GAAAGTCTGT TGGATGATCG TCATGCTCAT 
TCATCTCATA ATCGACATGA ACAACAACAA CAAACTGGAT CTCTTGGAAA AGTGTGCCGC 
TCTCACGATG AATGCTCTGA GCACGGAAGC TGCGCTTATC ACCACTCACT CGGTTATTAT 
CAATGCGCAT GTACCGAGCC ATATGTTGGC AATGGAGTCG AATGTACACT TCCAGGATCT 
TCTGCTTCAG CAGAATCCTC TACTAGGAGC GACAAGTTGC TAGTCGCCCG TGGAATGGCC 
ATATTTGAAC GCTCAACAAA TCCAGACGAG TACGGAAAAC AGCTGATTGT GATCCCTCAT 
CATATCCCAG TTGGCATTGA CTTTGATTGT AAAGAAGAAA AAATTGTGTG GAGTGACATG 
TCTGGTCACT CAATTCGCAC ATCTTCATTA AATGGAACTG AACATAAATC ATACTTCAAT 
AAAGAACTCA GCAGTCCTGA AGGAATTGCC GTTGACTGGT CTTCAAGAAA CGTGTACTAT 
GCTGACTCAA TGAATGATGA AATTGGAGTT GCTTCACTAA ATGGAAAATT CAAGAAATCT 
CTTGTAACTG AAGGTCTTGT CAATCCACGT TCAGTGGTTT TGGATCTATA CGGACGACAC 
TTGTACTACT CGGATTGGCA TCGTGAGAAT CCATACATCG GAAGAGTTGA CATGGATGGA 
AAGAACAATC GAGTATTCTT AAATGAAGAT GTCCATCTTC CAAATGGTCT CACTATTCTT 
CCAAACAGAC GCGAACTCTG TTGGGTGGAC GCTGGAAATC ACAGACTTTC ATGTATTCAG 
TACAATGGAG CTGGTAGAAG AACTGTGTTC AGTAGTCTTC AGTATCCGTT TGGTTTGACT 
CATGATGAAG AACAAAAATT CTATTGGACT GATTGGAAAG ACAACCGCAT TCATTCAGTT 
GGTGTCTATG GAGAAGGATA CCGTAGCTTC CAAATTTCCC TTGGTGGATC AGGAAAAGTA 
TTTGGAATCC TTGCCGTTCC AAAATCATGC GTCGGTCCAT CAACTCCATG TTCTGAAGAC 
AACGGTGGCT GCCAACATCT TTGCCTTCCA GGACAGAATG GTGCAGTTTG CGAATGTCCG 
GACAATGTCA AAGTGAAGGG ATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nid-1 ( NM_001269536.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256465.1 Caenorhabditis elegans NIDogen (basement membrane protein) (nid-1), partial mRNA.

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CloneID	OCa345672
Clone ID Related Accession (Same CDS sequence)	NM_001269536.1 , NM_001269536.2
Accession Version	NM_001269536.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	NIDogen (basement membrane protein)
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGAAACACA AAAAAATGTT GATATTACTA TTTCTATATC TGTTGATAGA AACAACGACA 
GCAAACCCTG CATTCTACCC GTATGGACAA CCGGCTGGAG ATAATAGACT TGACTATTCT 
ATGGAACAAA GTGCAATTGC TCTTAAAATC CCACTGACAT ATATGGAACA GTCCGTTGAT 
GAGCTCTTTC TCTCACACAA TGGAATTGTC GGTCTTGGAA AACCGACGCC GGAGAAGATT 
GGAGGCCTAA TGACAATCTC TACACCTGCC ATAGCAGTTT TCTACGTGCC GGTTACAAGT 
GGAGTAATTG ATTATAGAGT TAGCTCTGAT GATCAGGGAC TGTTGACAAA ACTGACGCAA 
GATGTGAAAC AAGTTTTTGC GGATGCTGTT GAGTTCCATG GATTGCAAGC AGTAATCATC 
ACGTGGACAC AAATTGAGAA TGCTGAGAAA GATGGGCCTG CTAGTTTCCA GCTGGCAATT 
GTGTCAGACG GAATTTCAAC GTATGCAATT TTCCGTTATG AATCACTCCC CTGGTCATCT 
TCAATGGGAT ACTACGCACA GGCGGGATTC GTCCGCTCCA TTGGAAAAAT TCAGACAAAT 
GTGAATTCCG GAGGACCGGA TGTTAAGGAA CTTGTAAATT TGTCCAATAA TCAGTTCGGT 
AACTTTTTCA TCTTCCGAGT TTCGGGATCA GCAATTGAAG ATCCAAAGGA TGAAGGAGCT 
GATGATTATG ATTACAACAA TTACGATCAG GAATACGAGG GCGAACATCC AGCCGATTGC 
CCAAAAGACA AATTTGTTGA CAACTGCCCA GCCGAGTGTA GAACTGTAAG AGATGAACGA 
GGATGTGAAA GATGTATTTG TGCAGAGAGA CCACATGCCC CAAGAGCTCC TCAAGAGCCA 
AGACCAGAAC TGGAGAACAC TGTTGAAGAC GATGAAGATG ATGAACAAGT TGATGGAACA 
AGATATGAAG AGCCAGCTAG ACAGGCTCAG GAGCAACATC ACCAGCAACA CCATCAGCCG 
GATCAACCTC AGCAGCAGCC GCAACGCAGT CAGGAAGGAT CATTACCGAA AATGTCGTGC 
TCTCAAAGAG ATGACAAGAG CTGTCATGCA AACTCGGTTT GTCAAGATTT CGAAGGCGGA 
TTCTGCTGTA ACTGTGACAC GGGATTCTAT GGAAACGGAA AAGAGTGTCT TCCAAAAGGT 
GAACCACAAA GAATTTCTGG AAGCTTCGAG GGAGTCATCA ACAGAATTCC AATTGACAAG 
ACTGAACTTC ACACTTTTGC AACTTCGACT GATGGAAATG TTCACACAGC TGTTTCAAAG 
ATTCCATCGG ATCTTGGACA TCCACTTCGT TTCCTTTACT CAATTGGAGG AGTCATGGGA 
TGGCTATTTG CCGACGTTCA ATCCCCGAAT GTCTACAATG GATTCCAACT GACCGGTGGA 
CTTTTCAATA GAACCGTTGC ACTTCATATT GAACAAAACT ATTACGTAAC GATTAAGCAA 
GAGTTCTCCG GAAGAAACAT TCATGATTAC TTCAAATCTC ATTTATTTGT ATCGGGAACT 
CTTCCTGATA TTGCTCCAGG ATCTGAAGTT ATCTTCCCAG ATTATGAAGA AGAATACGTT 
AGAGAAAGAC GAGGATACTT AACATCAAAA GCAGCTTTCG ATGTAATCGT TAGAGATGGA 
GGAAATGTTC AAACATATCG AATGAGTGTT GATCAACAAA TTACTTTTGA AGAATGCCCA 
AATAAAGAGT TTGATCGTGA TCATTCAATG AAGCTTCATG TGAAGAGAAT TAATGTTGTC 
TATAACGATG ACGAAGGAGT CGTCCGATAT GGAGCAAAAA ACTTTGCAAC TCGATCAGTT 
GGGCCTGCTG TTTCAGCTCC TTCCGGTGGG CATTTCGATC GTCGCCAACA CGGACAATCC 
AACATGGCTT CAGAAAGACC AATTGAAATT CCAAGCCAAA GTGAATCAAT CAGTACTGAC 
TCAGTTTGTG CTCCAGGAAG ACATCAATGT ACTCTTCCAA ATATGAAGTG CCGTGTCGTT 
GATCCATCAT ACCGTTGTGA ATGTGAACCT GGATATCAAG CAGCTCATGA TGCATCCTCT 
CATATTGGAT GGATCTGTCA AGATTTGGAT GAATGTCAAC GAGGAGATCA TAATTGTGAC 
CAGCATGCCA AGTGTACTAA TCGACCAGGA TCTTTCTCAT GCCAATGTCT TCAAGGATAT 
CAAGGAGATG GAAGATCTTG TATTCGGGAG CATCAAGCAT CTCATCACGA ACATAATCAA 
CAGACTCCAC AAGAAATGGC CGGTGTCGGA GCGACAACTG AAGGATTCTG CACTGCACAT 
AACCAATGCC ATCAATGGGG AGAATGTGTA TTCACTTCTG AGCATCCAAC CGGAAGATGC 
AAGTGTAGGG GATGGTATGT TGGAGATGGA GTTAATCATT GTGGACCACC AGAGGAGAAC 
ATGCCAAAAC ACAATGCAAA TATTCCGCAG AGAGGAGGCC AAGCTTGTGG AAGTTATGTA 
TGCGATGTGA ATGCTGAGTG TATGCCAGAA CCAAGTGGAG GATCTGAATG TGTATGCAAG 
GCCGGATTCT CAGGAAATGG AGTAACTTGT GAAAGTCTGT TGGATGATCG TCATGCTCAT 
TCATCTCATA ATCGACATGA ACAACAACAA CAAACTGGAT CTCTTGGAAA AGTGTGCCGC 
TCTCACGATG AATGCTCTGA GCACGGAAGC TGCGCTTATC ACCACTCACT CGGTTATTAT 
CAATGCGCAT GTACCGAGCC ATATGTTGGC AATGGAGTCG AATGTACACT TCCAGGATCT 
TCTGCTTCAG CTCCTGAACT CGGATGTGAT GTTACTCGAG ACTGCTCCGA GTTTGCTGAC 
TGTGTCTATG AAAGAAGTTC CACTGGTGCT ACATTCCGAT GCGTCTGTCA GTCAGGATAT 
ACTGGCGATG GAAAATATTG CATGCAATCT CAGCTTGCCA TATCCGCAAT TTCCCCCTCT 
GTTCCTCAGC TGCCAAGTGA GCCGGCCGTT TTATCAACAG CGTCATGTAA TCCAAACTGT 
GGACCTGATG CTCAATGTGT TTATGATGAC CATAATAGAC AATATCGATG TGAATGTTAT 
GCTGCATTCA TGGGAGATGG CTACAACTGT GTCCCCCTTG CAAAACCAAA TATGGTACCT 
GCACAACCAA AAACATGCGT CGAATCATCT GATTGTCATA TCAATGGACA CTGCGTTATC 
AATGAACATG GAGCTGGAGA ATACATTTGT CAATGTCTTC CTGGATTCAG TGGAGACGGA 
TTCATCAACT GTCGCGGAGC TGATCAATGT AACCCATCAA ACCCATCAGC TTGTTATCAG 
AATGCTCATT GTGTTTACGA TGCGATTCTG AATGCTCATG CCTGTAAATG TGTTGATGGA 
TTCAAGGGAG ATGGCACCAG TTGTGTTCCA TATGCACCTG CAACAAACTG CAATTTGGAG 
CCAAGAATTT GTCATGCAAA TGCACAATGC GTGATGCACC ATGATACAAA TGCTTATGAG 
TGCATCTGTA AACCAGGATC ATCTGGAGAC GGTTACACTA AATGTGATGT CATCGAAACT 
CCTCGTTGCA CCAACTGCTC AATCCATGCA TATTGTGCTC AAAATCCGAC TAGCGGTGCC 
TATCAATGTA AATGTAACGC AGGATATAAT GGAAATGGAC ATTTATGTGT CTCGATGAGC 
TCCTGCCTCG ATGACCGATC CCTCTGTGAT GAGAATGCCG ATTGTGTTCC GGGAGAAGCT 
GGGCATTACG TCTGCAACTG CCACTATGGA TACCATGGAG ATGGTAGATC ATGTTCCCCA 
GAATCCTCTA CTAGGAGCGA CAAGTTGCTA GTCGCCCGTG GAATGGCCAT ATTTGAACGC 
TCAACAAATC CAGACGAGTA CGGAAAACAG CTGATTGTGA TCCCTCATCA TATCCCAGTT 
GGCATTGACT TTGATTGTAA AGAAGAAAAA ATTGTGTGGA GTGACATGTC TGGTCACTCA 
ATTCGCACAT CTTCATTAAA TGGAACTGAA CATAAATCAT ACTTCAATAA AGAACTCAGC 
AGTCCTGAAG GAATTGCCGT TGACTGGTCT TCAAGAAACG TGTACTATGC TGACTCAATG 
AATGATGAAA TTGGAGTTGC TTCACTAAAT GGAAAATTCA AGAAATCTCT TGTAACTGAA 
GGTCTTGTCA ATCCACGTTC AGTGGTTTTG GATCTATACG GACGACACTT GTACTACTCG 
GATTGGCATC GTGAGAATCC ATACATCGGA AGAGTTGACA TGGATGGAAA GAACAATCGA 
GTATTCTTAA ATGAAGATGT CCATCTTCCA AATGGTCTCA CTATTCTTCC AAACAGACGC 
GAACTCTGTT GGGTGGACGC TGGAAATCAC AGACTTTCAT GTATTCAGTA CAATGGAGCT 
GGTAGAAGAA CTGTGTTCAG TAGTCTTCAG TATCCGTTTG GTTTGACTCA TGATGAAGAA 
CAAAAATTCT ATTGGACTGA TTGGAAAGAC AACCGCATTC ATTCAGTTGG TGTCTATGGA 
GAAGGATACC GTAGCTTCCA AATTTCCCTT GGTGGATCAG GAAAAGTATT TGGAATCCTT 
GCCGTTCCAA AATCATGCGT CGGTCCATCA ACTCCATGTT CTGAAGACAA CGGTGGCTGC 
CAACATCTTT GCCTTCCAGG ACAGAATGGT GCAGTTTGCG AATGTCCGGA CAATGTCAAA 
GTGAAGGGAT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001256465.1
CDS	1..4755
Translation	MKHKKMLILLFLYLLIETTTANPAFYPYGQPAGDNRLDYSMEQSAIALKIPLTYMEQSVDELFLSHNGIVGLGKPTPEKIGGLMTISTPAIAVFYVPVTSGVIDYRVSSDDQGLLTKLTQDVKQVFADAVEFHGLQAVIITWTQIENAEKDGPASFQLAIVSDGISTYAIFRYESLPWSSSMGYYAQAGFVRSIGKIQTNVNSGGPDVKELVNLSNNQFGNFFIFRVSGSAIEDPKDEGADDYDYNNYDQEYEGEHPADCPKDKFVDNCPAECRTVRDERGCERCICAERPHAPRAPQEPRPELENTVEDDEDDEQVDGTRYEEPARQAQEQHHQQHHQPDQPQQQPQRSQEGSLPKMSCSQRDDKSCHANSVCQDFEGGFCCNCDTGFYGNGKECLPKGEPQRISGSFEGVINRIPIDKTELHTFATSTDGNVHTAVSKIPSDLGHPLRFLYSIGGVMGWLFADVQSPNVYNGFQLTGGLFNRTVALHIEQNYYVTIKQEFSGRNIHDYFKSHLFVSGTLPDIAPGSEVIFPDYEEEYVRERRGYLTSKAAFDVIVRDGGNVQTYRMSVDQQITFEECPNKEFDRDHSMKLHVKRINVVYNDDEGVVRYGAKNFATRSVGPAVSAPSGGHFDRRQHGQSNMASERPIEIPSQSESISTDSVCAPGRHQCTLPNMKCRVVDPSYRCECEPGYQAAHDASSHIGWICQDLDECQRGDHNCDQHAKCTNRPGSFSCQCLQGYQGDGRSCIREHQASHHEHNQQTPQEMAGVGATTEGFCTAHNQCHQWGECVFTSEHPTGRCKCRGWYVGDGVNHCGPPEENMPKHNANIPQRGGQACGSYVCDVNAECMPEPSGGSECVCKAGFSGNGVTCESLLDDRHAHSSHNRHEQQQQTGSLGKVCRSHDECSEHGSCAYHHSLGYYQCACTEPYVGNGVECTLPGSSASAPELGCDVTRDCSEFADCVYERSSTGATFRCVCQSGYTGDGKYCMQSQLAISAISPSVPQLPSEPAVLSTASCNPNCGPDAQCVYDDHNRQYRCECYAAFMGDGYNCVPLAKPNMVPAQPKTCVESSDCHINGHCVINEHGAGEYICQCLPGFSGDGFINCRGADQCNPSNPSACYQNAHCVYDAILNAHACKCVDGFKGDGTSCVPYAPATNCNLEPRICHANAQCVMHHDTNAYECICKPGSSGDGYTKCDVIETPRCTNCSIHAYCAQNPTSGAYQCKCNAGYNGNGHLCVSMSSCLDDRSLCDENADCVPGEAGHYVCNCHYGYHGDGRSCSPESSTRSDKLLVARGMAIFERSTNPDEYGKQLIVIPHHIPVGIDFDCKEEKIVWSDMSGHSIRTSSLNGTEHKSYFNKELSSPEGIAVDWSSRNVYYADSMNDEIGVASLNGKFKKSLVTEGLVNPRSVVLDLYGRHLYYSDWHRENPYIGRVDMDGKNNRVFLNEDVHLPNGLTILPNRRELCWVDAGNHRLSCIQYNGAGRRTVFSSLQYPFGLTHDEEQKFYWTDWKDNRIHSVGVYGEGYRSFQISLGGSGKVFGILAVPKSCVGPSTPCSEDNGGCQHLCLPGQNGAVCECPDNVKVKGC

Target ORF information:

RefSeq Version	NM_001269536.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans NIDogen (basement membrane protein) (nid-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001269536.1

ORF Insert Sequence:

ATGAAACACA AAAAAATGTT GATATTACTA TTTCTATATC TGTTGATAGA AACAACGACA 
GCAAACCCTG CATTCTACCC GTATGGACAA CCGGCTGGAG ATAATAGACT TGACTATTCT 
ATGGAACAAA GTGCAATTGC TCTTAAAATC CCACTGACAT ATATGGAACA GTCCGTTGAT 
GAGCTCTTTC TCTCACACAA TGGAATTGTC GGTCTTGGAA AACCGACGCC GGAGAAGATT 
GGAGGCCTAA TGACAATCTC TACACCTGCC ATAGCAGTTT TCTACGTGCC GGTTACAAGT 
GGAGTAATTG ATTATAGAGT TAGCTCTGAT GATCAGGGAC TGTTGACAAA ACTGACGCAA 
GATGTGAAAC AAGTTTTTGC GGATGCTGTT GAGTTCCATG GATTGCAAGC AGTAATCATC 
ACGTGGACAC AAATTGAGAA TGCTGAGAAA GATGGGCCTG CTAGTTTCCA GCTGGCAATT 
GTGTCAGACG GAATTTCAAC GTATGCAATT TTCCGTTATG AATCACTCCC CTGGTCATCT 
TCAATGGGAT ACTACGCACA GGCGGGATTC GTCCGCTCCA TTGGAAAAAT TCAGACAAAT 
GTGAATTCCG GAGGACCGGA TGTTAAGGAA CTTGTAAATT TGTCCAATAA TCAGTTCGGT 
AACTTTTTCA TCTTCCGAGT TTCGGGATCA GCAATTGAAG ATCCAAAGGA TGAAGGAGCT 
GATGATTATG ATTACAACAA TTACGATCAG GAATACGAGG GCGAACATCC AGCCGATTGC 
CCAAAAGACA AATTTGTTGA CAACTGCCCA GCCGAGTGTA GAACTGTAAG AGATGAACGA 
GGATGTGAAA GATGTATTTG TGCAGAGAGA CCACATGCCC CAAGAGCTCC TCAAGAGCCA 
AGACCAGAAC TGGAGAACAC TGTTGAAGAC GATGAAGATG ATGAACAAGT TGATGGAACA 
AGATATGAAG AGCCAGCTAG ACAGGCTCAG GAGCAACATC ACCAGCAACA CCATCAGCCG 
GATCAACCTC AGCAGCAGCC GCAACGCAGT CAGGAAGGAT CATTACCGAA AATGTCGTGC 
TCTCAAAGAG ATGACAAGAG CTGTCATGCA AACTCGGTTT GTCAAGATTT CGAAGGCGGA 
TTCTGCTGTA ACTGTGACAC GGGATTCTAT GGAAACGGAA AAGAGTGTCT TCCAAAAGGT 
GAACCACAAA GAATTTCTGG AAGCTTCGAG GGAGTCATCA ACAGAATTCC AATTGACAAG 
ACTGAACTTC ACACTTTTGC AACTTCGACT GATGGAAATG TTCACACAGC TGTTTCAAAG 
ATTCCATCGG ATCTTGGACA TCCACTTCGT TTCCTTTACT CAATTGGAGG AGTCATGGGA 
TGGCTATTTG CCGACGTTCA ATCCCCGAAT GTCTACAATG GATTCCAACT GACCGGTGGA 
CTTTTCAATA GAACCGTTGC ACTTCATATT GAACAAAACT ATTACGTAAC GATTAAGCAA 
GAGTTCTCCG GAAGAAACAT TCATGATTAC TTCAAATCTC ATTTATTTGT ATCGGGAACT 
CTTCCTGATA TTGCTCCAGG ATCTGAAGTT ATCTTCCCAG ATTATGAAGA AGAATACGTT 
AGAGAAAGAC GAGGATACTT AACATCAAAA GCAGCTTTCG ATGTAATCGT TAGAGATGGA 
GGAAATGTTC AAACATATCG AATGAGTGTT GATCAACAAA TTACTTTTGA AGAATGCCCA 
AATAAAGAGT TTGATCGTGA TCATTCAATG AAGCTTCATG TGAAGAGAAT TAATGTTGTC 
TATAACGATG ACGAAGGAGT CGTCCGATAT GGAGCAAAAA ACTTTGCAAC TCGATCAGTT 
GGGCCTGCTG TTTCAGCTCC TTCCGGTGGG CATTTCGATC GTCGCCAACA CGGACAATCC 
AACATGGCTT CAGAAAGACC AATTGAAATT CCAAGCCAAA GTGAATCAAT CAGTACTGAC 
TCAGTTTGTG CTCCAGGAAG ACATCAATGT ACTCTTCCAA ATATGAAGTG CCGTGTCGTT 
GATCCATCAT ACCGTTGTGA ATGTGAACCT GGATATCAAG CAGCTCATGA TGCATCCTCT 
CATATTGGAT GGATCTGTCA AGATTTGGAT GAATGTCAAC GAGGAGATCA TAATTGTGAC 
CAGCATGCCA AGTGTACTAA TCGACCAGGA TCTTTCTCAT GCCAATGTCT TCAAGGATAT 
CAAGGAGATG GAAGATCTTG TATTCGGGAG CATCAAGCAT CTCATCACGA ACATAATCAA 
CAGACTCCAC AAGAAATGGC CGGTGTCGGA GCGACAACTG AAGGATTCTG CACTGCACAT 
AACCAATGCC ATCAATGGGG AGAATGTGTA TTCACTTCTG AGCATCCAAC CGGAAGATGC 
AAGTGTAGGG GATGGTATGT TGGAGATGGA GTTAATCATT GTGGACCACC AGAGGAGAAC 
ATGCCAAAAC ACAATGCAAA TATTCCGCAG AGAGGAGGCC AAGCTTGTGG AAGTTATGTA 
TGCGATGTGA ATGCTGAGTG TATGCCAGAA CCAAGTGGAG GATCTGAATG TGTATGCAAG 
GCCGGATTCT CAGGAAATGG AGTAACTTGT GAAAGTCTGT TGGATGATCG TCATGCTCAT 
TCATCTCATA ATCGACATGA ACAACAACAA CAAACTGGAT CTCTTGGAAA AGTGTGCCGC 
TCTCACGATG AATGCTCTGA GCACGGAAGC TGCGCTTATC ACCACTCACT CGGTTATTAT 
CAATGCGCAT GTACCGAGCC ATATGTTGGC AATGGAGTCG AATGTACACT TCCAGGATCT 
TCTGCTTCAG CTCCTGAACT CGGATGTGAT GTTACTCGAG ACTGCTCCGA GTTTGCTGAC 
TGTGTCTATG AAAGAAGTTC CACTGGTGCT ACATTCCGAT GCGTCTGTCA GTCAGGATAT 
ACTGGCGATG GAAAATATTG CATGCAATCT CAGCTTGCCA TATCCGCAAT TTCCCCCTCT 
GTTCCTCAGC TGCCAAGTGA GCCGGCCGTT TTATCAACAG CGTCATGTAA TCCAAACTGT 
GGACCTGATG CTCAATGTGT TTATGATGAC CATAATAGAC AATATCGATG TGAATGTTAT 
GCTGCATTCA TGGGAGATGG CTACAACTGT GTCCCCCTTG CAAAACCAAA TATGGTACCT 
GCACAACCAA AAACATGCGT CGAATCATCT GATTGTCATA TCAATGGACA CTGCGTTATC 
AATGAACATG GAGCTGGAGA ATACATTTGT CAATGTCTTC CTGGATTCAG TGGAGACGGA 
TTCATCAACT GTCGCGGAGC TGATCAATGT AACCCATCAA ACCCATCAGC TTGTTATCAG 
AATGCTCATT GTGTTTACGA TGCGATTCTG AATGCTCATG CCTGTAAATG TGTTGATGGA 
TTCAAGGGAG ATGGCACCAG TTGTGTTCCA TATGCACCTG CAACAAACTG CAATTTGGAG 
CCAAGAATTT GTCATGCAAA TGCACAATGC GTGATGCACC ATGATACAAA TGCTTATGAG 
TGCATCTGTA AACCAGGATC ATCTGGAGAC GGTTACACTA AATGTGATGT CATCGAAACT 
CCTCGTTGCA CCAACTGCTC AATCCATGCA TATTGTGCTC AAAATCCGAC TAGCGGTGCC 
TATCAATGTA AATGTAACGC AGGATATAAT GGAAATGGAC ATTTATGTGT CTCGATGAGC 
TCCTGCCTCG ATGACCGATC CCTCTGTGAT GAGAATGCCG ATTGTGTTCC GGGAGAAGCT 
GGGCATTACG TCTGCAACTG CCACTATGGA TACCATGGAG ATGGTAGATC ATGTTCCCCA 
GAATCCTCTA CTAGGAGCGA CAAGTTGCTA GTCGCCCGTG GAATGGCCAT ATTTGAACGC 
TCAACAAATC CAGACGAGTA CGGAAAACAG CTGATTGTGA TCCCTCATCA TATCCCAGTT 
GGCATTGACT TTGATTGTAA AGAAGAAAAA ATTGTGTGGA GTGACATGTC TGGTCACTCA 
ATTCGCACAT CTTCATTAAA TGGAACTGAA CATAAATCAT ACTTCAATAA AGAACTCAGC 
AGTCCTGAAG GAATTGCCGT TGACTGGTCT TCAAGAAACG TGTACTATGC TGACTCAATG 
AATGATGAAA TTGGAGTTGC TTCACTAAAT GGAAAATTCA AGAAATCTCT TGTAACTGAA 
GGTCTTGTCA ATCCACGTTC AGTGGTTTTG GATCTATACG GACGACACTT GTACTACTCG 
GATTGGCATC GTGAGAATCC ATACATCGGA AGAGTTGACA TGGATGGAAA GAACAATCGA 
GTATTCTTAA ATGAAGATGT CCATCTTCCA AATGGTCTCA CTATTCTTCC AAACAGACGC 
GAACTCTGTT GGGTGGACGC TGGAAATCAC AGACTTTCAT GTATTCAGTA CAATGGAGCT 
GGTAGAAGAA CTGTGTTCAG TAGTCTTCAG TATCCGTTTG GTTTGACTCA TGATGAAGAA 
CAAAAATTCT ATTGGACTGA TTGGAAAGAC AACCGCATTC ATTCAGTTGG TGTCTATGGA 
GAAGGATACC GTAGCTTCCA AATTTCCCTT GGTGGATCAG GAAAAGTATT TGGAATCCTT 
GCCGTTCCAA AATCATGCGT CGGTCCATCA ACTCCATGTT CTGAAGACAA CGGTGGCTGC 
CAACATCTTT GCCTTCCAGG ACAGAATGGT GCAGTTTGCG AATGTCCGGA CAATGTCAAA 
GTGAAGGGAT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nid-1 ( NM_001269536.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256465.1 Caenorhabditis elegans NIDogen (basement membrane protein) (nid-1), mRNA.

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CloneID	OCa345672
Clone ID Related Accession (Same CDS sequence)	NM_001269536.1 , NM_001269536.2
Accession Version	NM_001269536.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	NIDogen (basement membrane protein)
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001269536.1.

ATGAAACACA AAAAAATGTT GATATTACTA TTTCTATATC TGTTGATAGA AACAACGACA 
GCAAACCCTG CATTCTACCC GTATGGACAA CCGGCTGGAG ATAATAGACT TGACTATTCT 
ATGGAACAAA GTGCAATTGC TCTTAAAATC CCACTGACAT ATATGGAACA GTCCGTTGAT 
GAGCTCTTTC TCTCACACAA TGGAATTGTC GGTCTTGGAA AACCGACGCC GGAGAAGATT 
GGAGGCCTAA TGACAATCTC TACACCTGCC ATAGCAGTTT TCTACGTGCC GGTTACAAGT 
GGAGTAATTG ATTATAGAGT TAGCTCTGAT GATCAGGGAC TGTTGACAAA ACTGACGCAA 
GATGTGAAAC AAGTTTTTGC GGATGCTGTT GAGTTCCATG GATTGCAAGC AGTAATCATC 
ACGTGGACAC AAATTGAGAA TGCTGAGAAA GATGGGCCTG CTAGTTTCCA GCTGGCAATT 
GTGTCAGACG GAATTTCAAC GTATGCAATT TTCCGTTATG AATCACTCCC CTGGTCATCT 
TCAATGGGAT ACTACGCACA GGCGGGATTC GTCCGCTCCA TTGGAAAAAT TCAGACAAAT 
GTGAATTCCG GAGGACCGGA TGTTAAGGAA CTTGTAAATT TGTCCAATAA TCAGTTCGGT 
AACTTTTTCA TCTTCCGAGT TTCGGGATCA GCAATTGAAG ATCCAAAGGA TGAAGGAGCT 
GATGATTATG ATTACAACAA TTACGATCAG GAATACGAGG GCGAACATCC AGCCGATTGC 
CCAAAAGACA AATTTGTTGA CAACTGCCCA GCCGAGTGTA GAACTGTAAG AGATGAACGA 
GGATGTGAAA GATGTATTTG TGCAGAGAGA CCACATGCCC CAAGAGCTCC TCAAGAGCCA 
AGACCAGAAC TGGAGAACAC TGTTGAAGAC GATGAAGATG ATGAACAAGT TGATGGAACA 
AGATATGAAG AGCCAGCTAG ACAGGCTCAG GAGCAACATC ACCAGCAACA CCATCAGCCG 
GATCAACCTC AGCAGCAGCC GCAACGCAGT CAGGAAGGAT CATTACCGAA AATGTCGTGC 
TCTCAAAGAG ATGACAAGAG CTGTCATGCA AACTCGGTTT GTCAAGATTT CGAAGGCGGA 
TTCTGCTGTA ACTGTGACAC GGGATTCTAT GGAAACGGAA AAGAGTGTCT TCCAAAAGGT 
GAACCACAAA GAATTTCTGG AAGCTTCGAG GGAGTCATCA ACAGAATTCC AATTGACAAG 
ACTGAACTTC ACACTTTTGC AACTTCGACT GATGGAAATG TTCACACAGC TGTTTCAAAG 
ATTCCATCGG ATCTTGGACA TCCACTTCGT TTCCTTTACT CAATTGGAGG AGTCATGGGA 
TGGCTATTTG CCGACGTTCA ATCCCCGAAT GTCTACAATG GATTCCAACT GACCGGTGGA 
CTTTTCAATA GAACCGTTGC ACTTCATATT GAACAAAACT ATTACGTAAC GATTAAGCAA 
GAGTTCTCCG GAAGAAACAT TCATGATTAC TTCAAATCTC ATTTATTTGT ATCGGGAACT 
CTTCCTGATA TTGCTCCAGG ATCTGAAGTT ATCTTCCCAG ATTATGAAGA AGAATACGTT 
AGAGAAAGAC GAGGATACTT AACATCAAAA GCAGCTTTCG ATGTAATCGT TAGAGATGGA 
GGAAATGTTC AAACATATCG AATGAGTGTT GATCAACAAA TTACTTTTGA AGAATGCCCA 
AATAAAGAGT TTGATCGTGA TCATTCAATG AAGCTTCATG TGAAGAGAAT TAATGTTGTC 
TATAACGATG ACGAAGGAGT CGTCCGATAT GGAGCAAAAA ACTTTGCAAC TCGATCAGTT 
GGGCCTGCTG TTTCAGCTCC TTCCGGTGGG CATTTCGATC GTCGCCAACA CGGACAATCC 
AACATGGCTT CAGAAAGACC AATTGAAATT CCAAGCCAAA GTGAATCAAT CAGTACTGAC 
TCAGTTTGTG CTCCAGGAAG ACATCAATGT ACTCTTCCAA ATATGAAGTG CCGTGTCGTT 
GATCCATCAT ACCGTTGTGA ATGTGAACCT GGATATCAAG CAGCTCATGA TGCATCCTCT 
CATATTGGAT GGATCTGTCA AGATTTGGAT GAATGTCAAC GAGGAGATCA TAATTGTGAC 
CAGCATGCCA AGTGTACTAA TCGACCAGGA TCTTTCTCAT GCCAATGTCT TCAAGGATAT 
CAAGGAGATG GAAGATCTTG TATTCGGGAG CATCAAGCAT CTCATCACGA ACATAATCAA 
CAGACTCCAC AAGAAATGGC CGGTGTCGGA GCGACAACTG AAGGATTCTG CACTGCACAT 
AACCAATGCC ATCAATGGGG AGAATGTGTA TTCACTTCTG AGCATCCAAC CGGAAGATGC 
AAGTGTAGGG GATGGTATGT TGGAGATGGA GTTAATCATT GTGGACCACC AGAGGAGAAC 
ATGCCAAAAC ACAATGCAAA TATTCCGCAG AGAGGAGGCC AAGCTTGTGG AAGTTATGTA 
TGCGATGTGA ATGCTGAGTG TATGCCAGAA CCAAGTGGAG GATCTGAATG TGTATGCAAG 
GCCGGATTCT CAGGAAATGG AGTAACTTGT GAAAGTCTGT TGGATGATCG TCATGCTCAT 
TCATCTCATA ATCGACATGA ACAACAACAA CAAACTGGAT CTCTTGGAAA AGTGTGCCGC 
TCTCACGATG AATGCTCTGA GCACGGAAGC TGCGCTTATC ACCACTCACT CGGTTATTAT 
CAATGCGCAT GTACCGAGCC ATATGTTGGC AATGGAGTCG AATGTACACT TCCAGGATCT 
TCTGCTTCAG CTCCTGAACT CGGATGTGAT GTTACTCGAG ACTGCTCCGA GTTTGCTGAC 
TGTGTCTATG AAAGAAGTTC CACTGGTGCT ACATTCCGAT GCGTCTGTCA GTCAGGATAT 
ACTGGCGATG GAAAATATTG CATGCAATCT CAGCTTGCCA TATCCGCAAT TTCCCCCTCT 
GTTCCTCAGC TGCCAAGTGA GCCGGCCGTT TTATCAACAG CGTCATGTAA TCCAAACTGT 
GGACCTGATG CTCAATGTGT TTATGATGAC CATAATAGAC AATATCGATG TGAATGTTAT 
GCTGCATTCA TGGGAGATGG CTACAACTGT GTCCCCCTTG CAAAACCAAA TATGGTACCT 
GCACAACCAA AAACATGCGT CGAATCATCT GATTGTCATA TCAATGGACA CTGCGTTATC 
AATGAACATG GAGCTGGAGA ATACATTTGT CAATGTCTTC CTGGATTCAG TGGAGACGGA 
TTCATCAACT GTCGCGGAGC TGATCAATGT AACCCATCAA ACCCATCAGC TTGTTATCAG 
AATGCTCATT GTGTTTACGA TGCGATTCTG AATGCTCATG CCTGTAAATG TGTTGATGGA 
TTCAAGGGAG ATGGCACCAG TTGTGTTCCA TATGCACCTG CAACAAACTG CAATTTGGAG 
CCAAGAATTT GTCATGCAAA TGCACAATGC GTGATGCACC ATGATACAAA TGCTTATGAG 
TGCATCTGTA AACCAGGATC ATCTGGAGAC GGTTACACTA AATGTGATGT CATCGAAACT 
CCTCGTTGCA CCAACTGCTC AATCCATGCA TATTGTGCTC AAAATCCGAC TAGCGGTGCC 
TATCAATGTA AATGTAACGC AGGATATAAT GGAAATGGAC ATTTATGTGT CTCGATGAGC 
TCCTGCCTCG ATGACCGATC CCTCTGTGAT GAGAATGCCG ATTGTGTTCC GGGAGAAGCT 
GGGCATTACG TCTGCAACTG CCACTATGGA TACCATGGAG ATGGTAGATC ATGTTCCCCA 
GAATCCTCTA CTAGGAGCGA CAAGTTGCTA GTCGCCCGTG GAATGGCCAT ATTTGAACGC 
TCAACAAATC CAGACGAGTA CGGAAAACAG CTGATTGTGA TCCCTCATCA TATCCCAGTT 
GGCATTGACT TTGATTGTAA AGAAGAAAAA ATTGTGTGGA GTGACATGTC TGGTCACTCA 
ATTCGCACAT CTTCATTAAA TGGAACTGAA CATAAATCAT ACTTCAATAA AGAACTCAGC 
AGTCCTGAAG GAATTGCCGT TGACTGGTCT TCAAGAAACG TGTACTATGC TGACTCAATG 
AATGATGAAA TTGGAGTTGC TTCACTAAAT GGAAAATTCA AGAAATCTCT TGTAACTGAA 
GGTCTTGTCA ATCCACGTTC AGTGGTTTTG GATCTATACG GACGACACTT GTACTACTCG 
GATTGGCATC GTGAGAATCC ATACATCGGA AGAGTTGACA TGGATGGAAA GAACAATCGA 
GTATTCTTAA ATGAAGATGT CCATCTTCCA AATGGTCTCA CTATTCTTCC AAACAGACGC 
GAACTCTGTT GGGTGGACGC TGGAAATCAC AGACTTTCAT GTATTCAGTA CAATGGAGCT 
GGTAGAAGAA CTGTGTTCAG TAGTCTTCAG TATCCGTTTG GTTTGACTCA TGATGAAGAA 
CAAAAATTCT ATTGGACTGA TTGGAAAGAC AACCGCATTC ATTCAGTTGG TGTCTATGGA 
GAAGGATACC GTAGCTTCCA AATTTCCCTT GGTGGATCAG GAAAAGTATT TGGAATCCTT 
GCCGTTCCAA AATCATGCGT CGGTCCATCA ACTCCATGTT CTGAAGACAA CGGTGGCTGC 
CAACATCTTT GCCTTCCAGG ACAGAATGGT GCAGTTTGCG AATGTCCGGA CAATGTCAAA 
GTGAAGGGAT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001256465.1
CDS	6..4760
Translation	MKHKKMLILLFLYLLIETTTANPAFYPYGQPAGDNRLDYSMEQSAIALKIPLTYMEQSVDELFLSHNGIVGLGKPTPEKIGGLMTISTPAIAVFYVPVTSGVIDYRVSSDDQGLLTKLTQDVKQVFADAVEFHGLQAVIITWTQIENAEKDGPASFQLAIVSDGISTYAIFRYESLPWSSSMGYYAQAGFVRSIGKIQTNVNSGGPDVKELVNLSNNQFGNFFIFRVSGSAIEDPKDEGADDYDYNNYDQEYEGEHPADCPKDKFVDNCPAECRTVRDERGCERCICAERPHAPRAPQEPRPELENTVEDDEDDEQVDGTRYEEPARQAQEQHHQQHHQPDQPQQQPQRSQEGSLPKMSCSQRDDKSCHANSVCQDFEGGFCCNCDTGFYGNGKECLPKGEPQRISGSFEGVINRIPIDKTELHTFATSTDGNVHTAVSKIPSDLGHPLRFLYSIGGVMGWLFADVQSPNVYNGFQLTGGLFNRTVALHIEQNYYVTIKQEFSGRNIHDYFKSHLFVSGTLPDIAPGSEVIFPDYEEEYVRERRGYLTSKAAFDVIVRDGGNVQTYRMSVDQQITFEECPNKEFDRDHSMKLHVKRINVVYNDDEGVVRYGAKNFATRSVGPAVSAPSGGHFDRRQHGQSNMASERPIEIPSQSESISTDSVCAPGRHQCTLPNMKCRVVDPSYRCECEPGYQAAHDASSHIGWICQDLDECQRGDHNCDQHAKCTNRPGSFSCQCLQGYQGDGRSCIREHQASHHEHNQQTPQEMAGVGATTEGFCTAHNQCHQWGECVFTSEHPTGRCKCRGWYVGDGVNHCGPPEENMPKHNANIPQRGGQACGSYVCDVNAECMPEPSGGSECVCKAGFSGNGVTCESLLDDRHAHSSHNRHEQQQQTGSLGKVCRSHDECSEHGSCAYHHSLGYYQCACTEPYVGNGVECTLPGSSASAPELGCDVTRDCSEFADCVYERSSTGATFRCVCQSGYTGDGKYCMQSQLAISAISPSVPQLPSEPAVLSTASCNPNCGPDAQCVYDDHNRQYRCECYAAFMGDGYNCVPLAKPNMVPAQPKTCVESSDCHINGHCVINEHGAGEYICQCLPGFSGDGFINCRGADQCNPSNPSACYQNAHCVYDAILNAHACKCVDGFKGDGTSCVPYAPATNCNLEPRICHANAQCVMHHDTNAYECICKPGSSGDGYTKCDVIETPRCTNCSIHAYCAQNPTSGAYQCKCNAGYNGNGHLCVSMSSCLDDRSLCDENADCVPGEAGHYVCNCHYGYHGDGRSCSPESSTRSDKLLVARGMAIFERSTNPDEYGKQLIVIPHHIPVGIDFDCKEEKIVWSDMSGHSIRTSSLNGTEHKSYFNKELSSPEGIAVDWSSRNVYYADSMNDEIGVASLNGKFKKSLVTEGLVNPRSVVLDLYGRHLYYSDWHRENPYIGRVDMDGKNNRVFLNEDVHLPNGLTILPNRRELCWVDAGNHRLSCIQYNGAGRRTVFSSLQYPFGLTHDEEQKFYWTDWKDNRIHSVGVYGEGYRSFQISLGGSGKVFGILAVPKSCVGPSTPCSEDNGGCQHLCLPGQNGAVCECPDNVKVKGC

Target ORF information:

RefSeq Version	NM_001269536.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans NIDogen (basement membrane protein) (nid-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001269536.2

ORF Insert Sequence:

ATGAAACACA AAAAAATGTT GATATTACTA TTTCTATATC TGTTGATAGA AACAACGACA 
GCAAACCCTG CATTCTACCC GTATGGACAA CCGGCTGGAG ATAATAGACT TGACTATTCT 
ATGGAACAAA GTGCAATTGC TCTTAAAATC CCACTGACAT ATATGGAACA GTCCGTTGAT 
GAGCTCTTTC TCTCACACAA TGGAATTGTC GGTCTTGGAA AACCGACGCC GGAGAAGATT 
GGAGGCCTAA TGACAATCTC TACACCTGCC ATAGCAGTTT TCTACGTGCC GGTTACAAGT 
GGAGTAATTG ATTATAGAGT TAGCTCTGAT GATCAGGGAC TGTTGACAAA ACTGACGCAA 
GATGTGAAAC AAGTTTTTGC GGATGCTGTT GAGTTCCATG GATTGCAAGC AGTAATCATC 
ACGTGGACAC AAATTGAGAA TGCTGAGAAA GATGGGCCTG CTAGTTTCCA GCTGGCAATT 
GTGTCAGACG GAATTTCAAC GTATGCAATT TTCCGTTATG AATCACTCCC CTGGTCATCT 
TCAATGGGAT ACTACGCACA GGCGGGATTC GTCCGCTCCA TTGGAAAAAT TCAGACAAAT 
GTGAATTCCG GAGGACCGGA TGTTAAGGAA CTTGTAAATT TGTCCAATAA TCAGTTCGGT 
AACTTTTTCA TCTTCCGAGT TTCGGGATCA GCAATTGAAG ATCCAAAGGA TGAAGGAGCT 
GATGATTATG ATTACAACAA TTACGATCAG GAATACGAGG GCGAACATCC AGCCGATTGC 
CCAAAAGACA AATTTGTTGA CAACTGCCCA GCCGAGTGTA GAACTGTAAG AGATGAACGA 
GGATGTGAAA GATGTATTTG TGCAGAGAGA CCACATGCCC CAAGAGCTCC TCAAGAGCCA 
AGACCAGAAC TGGAGAACAC TGTTGAAGAC GATGAAGATG ATGAACAAGT TGATGGAACA 
AGATATGAAG AGCCAGCTAG ACAGGCTCAG GAGCAACATC ACCAGCAACA CCATCAGCCG 
GATCAACCTC AGCAGCAGCC GCAACGCAGT CAGGAAGGAT CATTACCGAA AATGTCGTGC 
TCTCAAAGAG ATGACAAGAG CTGTCATGCA AACTCGGTTT GTCAAGATTT CGAAGGCGGA 
TTCTGCTGTA ACTGTGACAC GGGATTCTAT GGAAACGGAA AAGAGTGTCT TCCAAAAGGT 
GAACCACAAA GAATTTCTGG AAGCTTCGAG GGAGTCATCA ACAGAATTCC AATTGACAAG 
ACTGAACTTC ACACTTTTGC AACTTCGACT GATGGAAATG TTCACACAGC TGTTTCAAAG 
ATTCCATCGG ATCTTGGACA TCCACTTCGT TTCCTTTACT CAATTGGAGG AGTCATGGGA 
TGGCTATTTG CCGACGTTCA ATCCCCGAAT GTCTACAATG GATTCCAACT GACCGGTGGA 
CTTTTCAATA GAACCGTTGC ACTTCATATT GAACAAAACT ATTACGTAAC GATTAAGCAA 
GAGTTCTCCG GAAGAAACAT TCATGATTAC TTCAAATCTC ATTTATTTGT ATCGGGAACT 
CTTCCTGATA TTGCTCCAGG ATCTGAAGTT ATCTTCCCAG ATTATGAAGA AGAATACGTT 
AGAGAAAGAC GAGGATACTT AACATCAAAA GCAGCTTTCG ATGTAATCGT TAGAGATGGA 
GGAAATGTTC AAACATATCG AATGAGTGTT GATCAACAAA TTACTTTTGA AGAATGCCCA 
AATAAAGAGT TTGATCGTGA TCATTCAATG AAGCTTCATG TGAAGAGAAT TAATGTTGTC 
TATAACGATG ACGAAGGAGT CGTCCGATAT GGAGCAAAAA ACTTTGCAAC TCGATCAGTT 
GGGCCTGCTG TTTCAGCTCC TTCCGGTGGG CATTTCGATC GTCGCCAACA CGGACAATCC 
AACATGGCTT CAGAAAGACC AATTGAAATT CCAAGCCAAA GTGAATCAAT CAGTACTGAC 
TCAGTTTGTG CTCCAGGAAG ACATCAATGT ACTCTTCCAA ATATGAAGTG CCGTGTCGTT 
GATCCATCAT ACCGTTGTGA ATGTGAACCT GGATATCAAG CAGCTCATGA TGCATCCTCT 
CATATTGGAT GGATCTGTCA AGATTTGGAT GAATGTCAAC GAGGAGATCA TAATTGTGAC 
CAGCATGCCA AGTGTACTAA TCGACCAGGA TCTTTCTCAT GCCAATGTCT TCAAGGATAT 
CAAGGAGATG GAAGATCTTG TATTCGGGAG CATCAAGCAT CTCATCACGA ACATAATCAA 
CAGACTCCAC AAGAAATGGC CGGTGTCGGA GCGACAACTG AAGGATTCTG CACTGCACAT 
AACCAATGCC ATCAATGGGG AGAATGTGTA TTCACTTCTG AGCATCCAAC CGGAAGATGC 
AAGTGTAGGG GATGGTATGT TGGAGATGGA GTTAATCATT GTGGACCACC AGAGGAGAAC 
ATGCCAAAAC ACAATGCAAA TATTCCGCAG AGAGGAGGCC AAGCTTGTGG AAGTTATGTA 
TGCGATGTGA ATGCTGAGTG TATGCCAGAA CCAAGTGGAG GATCTGAATG TGTATGCAAG 
GCCGGATTCT CAGGAAATGG AGTAACTTGT GAAAGTCTGT TGGATGATCG TCATGCTCAT 
TCATCTCATA ATCGACATGA ACAACAACAA CAAACTGGAT CTCTTGGAAA AGTGTGCCGC 
TCTCACGATG AATGCTCTGA GCACGGAAGC TGCGCTTATC ACCACTCACT CGGTTATTAT 
CAATGCGCAT GTACCGAGCC ATATGTTGGC AATGGAGTCG AATGTACACT TCCAGGATCT 
TCTGCTTCAG CTCCTGAACT CGGATGTGAT GTTACTCGAG ACTGCTCCGA GTTTGCTGAC 
TGTGTCTATG AAAGAAGTTC CACTGGTGCT ACATTCCGAT GCGTCTGTCA GTCAGGATAT 
ACTGGCGATG GAAAATATTG CATGCAATCT CAGCTTGCCA TATCCGCAAT TTCCCCCTCT 
GTTCCTCAGC TGCCAAGTGA GCCGGCCGTT TTATCAACAG CGTCATGTAA TCCAAACTGT 
GGACCTGATG CTCAATGTGT TTATGATGAC CATAATAGAC AATATCGATG TGAATGTTAT 
GCTGCATTCA TGGGAGATGG CTACAACTGT GTCCCCCTTG CAAAACCAAA TATGGTACCT 
GCACAACCAA AAACATGCGT CGAATCATCT GATTGTCATA TCAATGGACA CTGCGTTATC 
AATGAACATG GAGCTGGAGA ATACATTTGT CAATGTCTTC CTGGATTCAG TGGAGACGGA 
TTCATCAACT GTCGCGGAGC TGATCAATGT AACCCATCAA ACCCATCAGC TTGTTATCAG 
AATGCTCATT GTGTTTACGA TGCGATTCTG AATGCTCATG CCTGTAAATG TGTTGATGGA 
TTCAAGGGAG ATGGCACCAG TTGTGTTCCA TATGCACCTG CAACAAACTG CAATTTGGAG 
CCAAGAATTT GTCATGCAAA TGCACAATGC GTGATGCACC ATGATACAAA TGCTTATGAG 
TGCATCTGTA AACCAGGATC ATCTGGAGAC GGTTACACTA AATGTGATGT CATCGAAACT 
CCTCGTTGCA CCAACTGCTC AATCCATGCA TATTGTGCTC AAAATCCGAC TAGCGGTGCC 
TATCAATGTA AATGTAACGC AGGATATAAT GGAAATGGAC ATTTATGTGT CTCGATGAGC 
TCCTGCCTCG ATGACCGATC CCTCTGTGAT GAGAATGCCG ATTGTGTTCC GGGAGAAGCT 
GGGCATTACG TCTGCAACTG CCACTATGGA TACCATGGAG ATGGTAGATC ATGTTCCCCA 
GAATCCTCTA CTAGGAGCGA CAAGTTGCTA GTCGCCCGTG GAATGGCCAT ATTTGAACGC 
TCAACAAATC CAGACGAGTA CGGAAAACAG CTGATTGTGA TCCCTCATCA TATCCCAGTT 
GGCATTGACT TTGATTGTAA AGAAGAAAAA ATTGTGTGGA GTGACATGTC TGGTCACTCA 
ATTCGCACAT CTTCATTAAA TGGAACTGAA CATAAATCAT ACTTCAATAA AGAACTCAGC 
AGTCCTGAAG GAATTGCCGT TGACTGGTCT TCAAGAAACG TGTACTATGC TGACTCAATG 
AATGATGAAA TTGGAGTTGC TTCACTAAAT GGAAAATTCA AGAAATCTCT TGTAACTGAA 
GGTCTTGTCA ATCCACGTTC AGTGGTTTTG GATCTATACG GACGACACTT GTACTACTCG 
GATTGGCATC GTGAGAATCC ATACATCGGA AGAGTTGACA TGGATGGAAA GAACAATCGA 
GTATTCTTAA ATGAAGATGT CCATCTTCCA AATGGTCTCA CTATTCTTCC AAACAGACGC 
GAACTCTGTT GGGTGGACGC TGGAAATCAC AGACTTTCAT GTATTCAGTA CAATGGAGCT 
GGTAGAAGAA CTGTGTTCAG TAGTCTTCAG TATCCGTTTG GTTTGACTCA TGATGAAGAA 
CAAAAATTCT ATTGGACTGA TTGGAAAGAC AACCGCATTC ATTCAGTTGG TGTCTATGGA 
GAAGGATACC GTAGCTTCCA AATTTCCCTT GGTGGATCAG GAAAAGTATT TGGAATCCTT 
GCCGTTCCAA AATCATGCGT CGGTCCATCA ACTCCATGTT CTGAAGACAA CGGTGGCTGC 
CAACATCTTT GCCTTCCAGG ACAGAATGGT GCAGTTTGCG AATGTCCGGA CAATGTCAAA 
GTGAAGGGAT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nid-1 ( NM_001269537.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256466.1 Caenorhabditis elegans NIDogen (basement membrane protein) (nid-1), partial mRNA.

Price & Availability↑

CloneID	OCa346023
Clone ID Related Accession (Same CDS sequence)	NM_001269537.1 , NM_001269537.2
Accession Version	NM_001269537.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	NIDogen (basement membrane protein)
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGAAACACA AAAAAATGTT GATATTACTA TTTCTATATC TGTTGATAGA AACAACGACA 
GCAAACCCTG CATTCTACCC GTATGGACAA CCGGCTGGAG ATAATAGACT TGACTATTCT 
ATGGAACAAA GTGCAATTGC TCTTAAAATC CCACTGACAT ATATGGAACA GTCCGTTGAT 
GAGCTCTTTC TCTCACACAA TGGAATTGTC GGTCTTGGAA AACCGACGCC GGAGAAGATT 
GGAGGCCTAA TGACAATCTC TACACCTGCC ATAGCAGTTT TCTACGTGCC GGTTACAAGT 
GGAGTAATTG ATTATAGAGT TAGCTCTGAT GATCAGGGAC TGTTGACAAA ACTGACGCAA 
GATGTGAAAC AAGTTTTTGC GGATGCTGTT GAGTTCCATG GATTGCAAGC AGTAATCATC 
ACGTGGACAC AAATTGAGAA TGCTGAGAAA GATGGGCCTG CTAGTTTCCA GCTGGCAATT 
GTGTCAGACG GAATTTCAAC GTATGCAATT TTCCGTTATG AATCACTCCC CTGGTCATCT 
TCAATGGGAT ACTACGCACA GGCGGGATTC GTCCGCTCCA TTGGAAAAAT TCAGACAAAT 
GTGAATTCCG GAGGACCGGA TGTTAAGGAA CTTGTAAATT TGTCCAATAA TCAGTTCGGT 
AACTTTTTCA TCTTCCGAGT TTCGGGATCA GCAATTGAAG ATCCAAAGGA TGAAGGAGCT 
GATGATTATG ATTACAACAA TTACGATCAG GAATACGAGG GCGAACATCC AGCCGATTGC 
CCAAAAGACA AATTTGTTGA CAACTGCCCA GCCGAGTGTA GAACTGTAAG AGATGAACGA 
GGATGTGAAA GATGTATTTG TGCAGAGAGA CCACATGCCC CAAGAGCTCC TCAAGAGCCA 
AGACCAGAAC TGGAGAACAC TGTTGAAGAC GATGAAGATG ATGAACAAGT TGATGGAACA 
AGATATGAAG AGCCAGCTAG ACAGGCTCAG GAGCAACATC ACCAGCAACA CCATCAGCCG 
GATCAACCTC AGCAGCAGCC GCAACGCAGT CAGGAAGGAT CATTACCGAA AATGTCGTGC 
TCTCAAAGAG ATGACAAGAG CTGTCATGCA AACTCGGTTT GTCAAGATTT CGAAGGCGGA 
TTCTGCTGTA ACTGTGACAC GGGATTCTAT GGAAACGGAA AAGAGTGTCT TCCAAAAGGT 
GAACCACAAA GAATTTCTGG AAGCTTCGAG GGAGTCATCA ACAGAATTCC AATTGACAAG 
ACTGAACTTC ACACTTTTGC AACTTCGACT GATGGAAATG TTCACACAGC TGTTTCAAAG 
ATTCCATCGG ATCTTGGACA TCCACTTCGT TTCCTTTACT CAATTGGAGG AGTCATGGGA 
TGGCTATTTG CCGACGTTCA ATCCCCGAAT GTCTACAATG GATTCCAACT GACCGGTGGA 
CTTTTCAATA GAACCGTTGC ACTTCATATT GAACAAAACT ATTACGTAAC GATTAAGCAA 
GAGTTCTCCG GAAGAAACAT TCATGATTAC TTCAAATCTC ATTTATTTGT ATCGGGAACT 
CTTCCTGATA TTGCTCCAGG ATCTGAAGTT ATCTTCCCAG ATTATGAAGA AGAATACGTT 
AGAGAAAGAC GAGGATACTT AACATCAAAA GCAGCTTTCG ATGTAATCGT TAGAGATGGA 
GGAAATGTTC AAACATATCG AATGAGTGTT GATCAACAAA TTACTTTTGA AGAATGCCCA 
AATAAAGAGT TTGATCGTGA TCATTCAATG AAGCTTCATG TGAAGAGAAT TAATGTTGTC 
TATAACGATG ACGAAGGAGT CGTCCGATAT GGAGCAAAAA ACTTTGCAAC TCGATCAGTT 
GGGCCTGCTG TTTCAGCTCC TTCCGGTGGG CATTTCGATC GTCGCCAACA CGGACAATCC 
AACATGGCTT CAGAAAGACC AATTGAAATT CCAAGCCAAA GTGAATCAAT CAGTACTGAC 
TCAGTTTGTG CTCCAGGAAG ACATCAATGT ACTCTTCCAA ATATGAAGTG CCGTGTCGTT 
GATCCATCAT ACCGTTGTGA ATGTGAACCT GGATATCAAG CAGCTCATGA TGCATCCTCT 
CATATTGGAT GGATCTGTCA AGATTTGGAT GAATGTCAAC GAGGAGATCA TAATTGTGAC 
CAGCATGCCA AGTGTACTAA TCGACCAGGA TCTTTCTCAT GCCAATGTCT TCAAGGATAT 
CAAGGAGATG GAAGATCTTG TATTCGGGAG CATCAAGCAT CTCATCACGA ACATAATCAA 
CAGACTCCAC AAGAAATGGC CGGTGTCGGA GCGACAACTG AAGGATTCTG CACTGCACAT 
AACCAATGCC ATCAATGGGG AGAATGTGTA TTCACTTCTG AGCATCCAAC CGGAAGATGC 
AAGTGTAGGG GATGGTATGT TGGAGATGGA GTTAATCATT GTGGACCACC AGAGGAGAAC 
ATGCCAAAAC ACAATGCAAA TATTCCGCAG AGAGGAGGCC AAGCTTGTGG AAGTTATGTA 
TGCGATGTGA ATGCTGAGTG TATGCCAGAA CCAAGTGGAG GATCTGAATG TGTATGCAAG 
GCCGGATTCT CAGGAAATGG AGTAACTTGT GAAAGTCTGT TGGATGATCG TCATGCTCAT 
TCATCTCATA ATCGACATGA ACAACAACAA CAAACTGGAT CTCTTGGAAA AGTGTGCCGC 
TCTCACGATG AATGCTCTGA GCACGGAAGC TGCGCTTATC ACCACTCACT CGGTTATTAT 
CAATGCGCAT GTACCGAGCC ATATGTTGGC AATGGAGTCG AATGTACACT TCCAGGATCT 
TCTGCTTCAG TTCCTCAGCT GCCAAGTGAG CCGGCCGTTT TATCAACAGC GTCATGTAAT 
CCAAACTGTG GACCTGATGC TCAATGTGTT TATGATGACC ATAATAGACA ATATCGATGT 
GAATGTTATG CTGCATTCAT GGGAGATGGC TACAACTGTG TCCCCCTTGC AAAACCAAAT 
ATGGTACCTG CACAACCAAA AACATGCGTC GAATCATCTG ATTGTCATAT CAATGGACAC 
TGCGTTATCA ATGAACATGG AGCTGGAGAA TACATTTGTC AATGTCTTCC TGGATTCAGT 
GGAGACGGAT TCATCAACTG TCGCGGAGCT GATCAATGTA ACCCATCAAA CCCATCAGCT 
TGTTATCAGA ATGCTCATTG TGTTTACGAT GCGATTCTGA ATGCTCATGC CTGTAAATGT 
GTTGATGGAT TCAAGGGAGA TGGCACCAGT TGTGTTCCAT ATGCACCTGC AACAAACTGC 
AATTTGGAGC CAAGAATTTG TCATGCAAAT GCACAATGCG TGATGCACCA TGATACAAAT 
GCTTATGAGT GCATCTGTAA ACCAGGATCA TCTGGAGACG GTTACACTAA ATGTGATGTC 
ATCGAAACTC CTCGTTGCAC CAACTGCTCA ATCCATGCAT ATTGTGCTCA AAATCCGACT 
AGCGGTGCCT ATCAATGTAA ATGTAACGCA GGATATAATG GAAATGGACA TTTATGTGTC 
TCGATGAGCT CCTGCCTCGA TGACCGATCC CTCTGTGATG AGAATGCCGA TTGTGTTCCG 
GGAGAAGCTG GGCATTACGT CTGCAACTGC CACTATGGAT ACCATGGAGA TGGTAGATCA 
TGTTCCCCAG AATCCTCTAC TAGGAGCGAC AAGTTGCTAG TCGCCCGTGG AATGGCCATA 
TTTGAACGCT CAACAAATCC AGACGAGTAC GGAAAACAGC TGATTGTGAT CCCTCATCAT 
ATCCCAGTTG GCATTGACTT TGATTGTAAA GAAGAAAAAA TTGTGTGGAG TGACATGTCT 
GGTCACTCAA TTCGCACATC TTCATTAAAT GGAACTGAAC ATAAATCATA CTTCAATAAA 
GAACTCAGCA GTCCTGAAGG AATTGCCGTT GACTGGTCTT CAAGAAACGT GTACTATGCT 
GACTCAATGA ATGATGAAAT TGGAGTTGCT TCACTAAATG GAAAATTCAA GAAATCTCTT 
GTAACTGAAG GTCTTGTCAA TCCACGTTCA GTGGTTTTGG ATCTATACGG ACGACACTTG 
TACTACTCGG ATTGGCATCG TGAGAATCCA TACATCGGAA GAGTTGACAT GGATGGAAAG 
AACAATCGAG TATTCTTAAA TGAAGATGTC CATCTTCCAA ATGGTCTCAC TATTCTTCCA 
AACAGACGCG AACTCTGTTG GGTGGACGCT GGAAATCACA GACTTTCATG TATTCAGTAC 
AATGGAGCTG GTAGAAGAAC TGTGTTCAGT AGTCTTCAGT ATCCGTTTGG TTTGACTCAT 
GATGAAGAAC AAAAATTCTA TTGGACTGAT TGGAAAGACA ACCGCATTCA TTCAGTTGGT 
GTCTATGGAG AAGGATACCG TAGCTTCCAA ATTTCCCTTG GTGGATCAGG AAAAGTATTT 
GGAATCCTTG CCGTTCCAAA ATCATGCGTC GGTCCATCAA CTCCATGTTC TGAAGACAAC 
GGTGGCTGCC AACATCTTTG CCTTCCAGGA CAGAATGGTG CAGTTTGCGA ATGTCCGGAC 
AATGTCAAAG TGAAGGGATG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001256466.1
CDS	1..4584
Translation	MKHKKMLILLFLYLLIETTTANPAFYPYGQPAGDNRLDYSMEQSAIALKIPLTYMEQSVDELFLSHNGIVGLGKPTPEKIGGLMTISTPAIAVFYVPVTSGVIDYRVSSDDQGLLTKLTQDVKQVFADAVEFHGLQAVIITWTQIENAEKDGPASFQLAIVSDGISTYAIFRYESLPWSSSMGYYAQAGFVRSIGKIQTNVNSGGPDVKELVNLSNNQFGNFFIFRVSGSAIEDPKDEGADDYDYNNYDQEYEGEHPADCPKDKFVDNCPAECRTVRDERGCERCICAERPHAPRAPQEPRPELENTVEDDEDDEQVDGTRYEEPARQAQEQHHQQHHQPDQPQQQPQRSQEGSLPKMSCSQRDDKSCHANSVCQDFEGGFCCNCDTGFYGNGKECLPKGEPQRISGSFEGVINRIPIDKTELHTFATSTDGNVHTAVSKIPSDLGHPLRFLYSIGGVMGWLFADVQSPNVYNGFQLTGGLFNRTVALHIEQNYYVTIKQEFSGRNIHDYFKSHLFVSGTLPDIAPGSEVIFPDYEEEYVRERRGYLTSKAAFDVIVRDGGNVQTYRMSVDQQITFEECPNKEFDRDHSMKLHVKRINVVYNDDEGVVRYGAKNFATRSVGPAVSAPSGGHFDRRQHGQSNMASERPIEIPSQSESISTDSVCAPGRHQCTLPNMKCRVVDPSYRCECEPGYQAAHDASSHIGWICQDLDECQRGDHNCDQHAKCTNRPGSFSCQCLQGYQGDGRSCIREHQASHHEHNQQTPQEMAGVGATTEGFCTAHNQCHQWGECVFTSEHPTGRCKCRGWYVGDGVNHCGPPEENMPKHNANIPQRGGQACGSYVCDVNAECMPEPSGGSECVCKAGFSGNGVTCESLLDDRHAHSSHNRHEQQQQTGSLGKVCRSHDECSEHGSCAYHHSLGYYQCACTEPYVGNGVECTLPGSSASVPQLPSEPAVLSTASCNPNCGPDAQCVYDDHNRQYRCECYAAFMGDGYNCVPLAKPNMVPAQPKTCVESSDCHINGHCVINEHGAGEYICQCLPGFSGDGFINCRGADQCNPSNPSACYQNAHCVYDAILNAHACKCVDGFKGDGTSCVPYAPATNCNLEPRICHANAQCVMHHDTNAYECICKPGSSGDGYTKCDVIETPRCTNCSIHAYCAQNPTSGAYQCKCNAGYNGNGHLCVSMSSCLDDRSLCDENADCVPGEAGHYVCNCHYGYHGDGRSCSPESSTRSDKLLVARGMAIFERSTNPDEYGKQLIVIPHHIPVGIDFDCKEEKIVWSDMSGHSIRTSSLNGTEHKSYFNKELSSPEGIAVDWSSRNVYYADSMNDEIGVASLNGKFKKSLVTEGLVNPRSVVLDLYGRHLYYSDWHRENPYIGRVDMDGKNNRVFLNEDVHLPNGLTILPNRRELCWVDAGNHRLSCIQYNGAGRRTVFSSLQYPFGLTHDEEQKFYWTDWKDNRIHSVGVYGEGYRSFQISLGGSGKVFGILAVPKSCVGPSTPCSEDNGGCQHLCLPGQNGAVCECPDNVKVKGC

Target ORF information:

RefSeq Version	NM_001269537.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans NIDogen (basement membrane protein) (nid-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001269537.1

ORF Insert Sequence:

ATGAAACACA AAAAAATGTT GATATTACTA TTTCTATATC TGTTGATAGA AACAACGACA 
GCAAACCCTG CATTCTACCC GTATGGACAA CCGGCTGGAG ATAATAGACT TGACTATTCT 
ATGGAACAAA GTGCAATTGC TCTTAAAATC CCACTGACAT ATATGGAACA GTCCGTTGAT 
GAGCTCTTTC TCTCACACAA TGGAATTGTC GGTCTTGGAA AACCGACGCC GGAGAAGATT 
GGAGGCCTAA TGACAATCTC TACACCTGCC ATAGCAGTTT TCTACGTGCC GGTTACAAGT 
GGAGTAATTG ATTATAGAGT TAGCTCTGAT GATCAGGGAC TGTTGACAAA ACTGACGCAA 
GATGTGAAAC AAGTTTTTGC GGATGCTGTT GAGTTCCATG GATTGCAAGC AGTAATCATC 
ACGTGGACAC AAATTGAGAA TGCTGAGAAA GATGGGCCTG CTAGTTTCCA GCTGGCAATT 
GTGTCAGACG GAATTTCAAC GTATGCAATT TTCCGTTATG AATCACTCCC CTGGTCATCT 
TCAATGGGAT ACTACGCACA GGCGGGATTC GTCCGCTCCA TTGGAAAAAT TCAGACAAAT 
GTGAATTCCG GAGGACCGGA TGTTAAGGAA CTTGTAAATT TGTCCAATAA TCAGTTCGGT 
AACTTTTTCA TCTTCCGAGT TTCGGGATCA GCAATTGAAG ATCCAAAGGA TGAAGGAGCT 
GATGATTATG ATTACAACAA TTACGATCAG GAATACGAGG GCGAACATCC AGCCGATTGC 
CCAAAAGACA AATTTGTTGA CAACTGCCCA GCCGAGTGTA GAACTGTAAG AGATGAACGA 
GGATGTGAAA GATGTATTTG TGCAGAGAGA CCACATGCCC CAAGAGCTCC TCAAGAGCCA 
AGACCAGAAC TGGAGAACAC TGTTGAAGAC GATGAAGATG ATGAACAAGT TGATGGAACA 
AGATATGAAG AGCCAGCTAG ACAGGCTCAG GAGCAACATC ACCAGCAACA CCATCAGCCG 
GATCAACCTC AGCAGCAGCC GCAACGCAGT CAGGAAGGAT CATTACCGAA AATGTCGTGC 
TCTCAAAGAG ATGACAAGAG CTGTCATGCA AACTCGGTTT GTCAAGATTT CGAAGGCGGA 
TTCTGCTGTA ACTGTGACAC GGGATTCTAT GGAAACGGAA AAGAGTGTCT TCCAAAAGGT 
GAACCACAAA GAATTTCTGG AAGCTTCGAG GGAGTCATCA ACAGAATTCC AATTGACAAG 
ACTGAACTTC ACACTTTTGC AACTTCGACT GATGGAAATG TTCACACAGC TGTTTCAAAG 
ATTCCATCGG ATCTTGGACA TCCACTTCGT TTCCTTTACT CAATTGGAGG AGTCATGGGA 
TGGCTATTTG CCGACGTTCA ATCCCCGAAT GTCTACAATG GATTCCAACT GACCGGTGGA 
CTTTTCAATA GAACCGTTGC ACTTCATATT GAACAAAACT ATTACGTAAC GATTAAGCAA 
GAGTTCTCCG GAAGAAACAT TCATGATTAC TTCAAATCTC ATTTATTTGT ATCGGGAACT 
CTTCCTGATA TTGCTCCAGG ATCTGAAGTT ATCTTCCCAG ATTATGAAGA AGAATACGTT 
AGAGAAAGAC GAGGATACTT AACATCAAAA GCAGCTTTCG ATGTAATCGT TAGAGATGGA 
GGAAATGTTC AAACATATCG AATGAGTGTT GATCAACAAA TTACTTTTGA AGAATGCCCA 
AATAAAGAGT TTGATCGTGA TCATTCAATG AAGCTTCATG TGAAGAGAAT TAATGTTGTC 
TATAACGATG ACGAAGGAGT CGTCCGATAT GGAGCAAAAA ACTTTGCAAC TCGATCAGTT 
GGGCCTGCTG TTTCAGCTCC TTCCGGTGGG CATTTCGATC GTCGCCAACA CGGACAATCC 
AACATGGCTT CAGAAAGACC AATTGAAATT CCAAGCCAAA GTGAATCAAT CAGTACTGAC 
TCAGTTTGTG CTCCAGGAAG ACATCAATGT ACTCTTCCAA ATATGAAGTG CCGTGTCGTT 
GATCCATCAT ACCGTTGTGA ATGTGAACCT GGATATCAAG CAGCTCATGA TGCATCCTCT 
CATATTGGAT GGATCTGTCA AGATTTGGAT GAATGTCAAC GAGGAGATCA TAATTGTGAC 
CAGCATGCCA AGTGTACTAA TCGACCAGGA TCTTTCTCAT GCCAATGTCT TCAAGGATAT 
CAAGGAGATG GAAGATCTTG TATTCGGGAG CATCAAGCAT CTCATCACGA ACATAATCAA 
CAGACTCCAC AAGAAATGGC CGGTGTCGGA GCGACAACTG AAGGATTCTG CACTGCACAT 
AACCAATGCC ATCAATGGGG AGAATGTGTA TTCACTTCTG AGCATCCAAC CGGAAGATGC 
AAGTGTAGGG GATGGTATGT TGGAGATGGA GTTAATCATT GTGGACCACC AGAGGAGAAC 
ATGCCAAAAC ACAATGCAAA TATTCCGCAG AGAGGAGGCC AAGCTTGTGG AAGTTATGTA 
TGCGATGTGA ATGCTGAGTG TATGCCAGAA CCAAGTGGAG GATCTGAATG TGTATGCAAG 
GCCGGATTCT CAGGAAATGG AGTAACTTGT GAAAGTCTGT TGGATGATCG TCATGCTCAT 
TCATCTCATA ATCGACATGA ACAACAACAA CAAACTGGAT CTCTTGGAAA AGTGTGCCGC 
TCTCACGATG AATGCTCTGA GCACGGAAGC TGCGCTTATC ACCACTCACT CGGTTATTAT 
CAATGCGCAT GTACCGAGCC ATATGTTGGC AATGGAGTCG AATGTACACT TCCAGGATCT 
TCTGCTTCAG TTCCTCAGCT GCCAAGTGAG CCGGCCGTTT TATCAACAGC GTCATGTAAT 
CCAAACTGTG GACCTGATGC TCAATGTGTT TATGATGACC ATAATAGACA ATATCGATGT 
GAATGTTATG CTGCATTCAT GGGAGATGGC TACAACTGTG TCCCCCTTGC AAAACCAAAT 
ATGGTACCTG CACAACCAAA AACATGCGTC GAATCATCTG ATTGTCATAT CAATGGACAC 
TGCGTTATCA ATGAACATGG AGCTGGAGAA TACATTTGTC AATGTCTTCC TGGATTCAGT 
GGAGACGGAT TCATCAACTG TCGCGGAGCT GATCAATGTA ACCCATCAAA CCCATCAGCT 
TGTTATCAGA ATGCTCATTG TGTTTACGAT GCGATTCTGA ATGCTCATGC CTGTAAATGT 
GTTGATGGAT TCAAGGGAGA TGGCACCAGT TGTGTTCCAT ATGCACCTGC AACAAACTGC 
AATTTGGAGC CAAGAATTTG TCATGCAAAT GCACAATGCG TGATGCACCA TGATACAAAT 
GCTTATGAGT GCATCTGTAA ACCAGGATCA TCTGGAGACG GTTACACTAA ATGTGATGTC 
ATCGAAACTC CTCGTTGCAC CAACTGCTCA ATCCATGCAT ATTGTGCTCA AAATCCGACT 
AGCGGTGCCT ATCAATGTAA ATGTAACGCA GGATATAATG GAAATGGACA TTTATGTGTC 
TCGATGAGCT CCTGCCTCGA TGACCGATCC CTCTGTGATG AGAATGCCGA TTGTGTTCCG 
GGAGAAGCTG GGCATTACGT CTGCAACTGC CACTATGGAT ACCATGGAGA TGGTAGATCA 
TGTTCCCCAG AATCCTCTAC TAGGAGCGAC AAGTTGCTAG TCGCCCGTGG AATGGCCATA 
TTTGAACGCT CAACAAATCC AGACGAGTAC GGAAAACAGC TGATTGTGAT CCCTCATCAT 
ATCCCAGTTG GCATTGACTT TGATTGTAAA GAAGAAAAAA TTGTGTGGAG TGACATGTCT 
GGTCACTCAA TTCGCACATC TTCATTAAAT GGAACTGAAC ATAAATCATA CTTCAATAAA 
GAACTCAGCA GTCCTGAAGG AATTGCCGTT GACTGGTCTT CAAGAAACGT GTACTATGCT 
GACTCAATGA ATGATGAAAT TGGAGTTGCT TCACTAAATG GAAAATTCAA GAAATCTCTT 
GTAACTGAAG GTCTTGTCAA TCCACGTTCA GTGGTTTTGG ATCTATACGG ACGACACTTG 
TACTACTCGG ATTGGCATCG TGAGAATCCA TACATCGGAA GAGTTGACAT GGATGGAAAG 
AACAATCGAG TATTCTTAAA TGAAGATGTC CATCTTCCAA ATGGTCTCAC TATTCTTCCA 
AACAGACGCG AACTCTGTTG GGTGGACGCT GGAAATCACA GACTTTCATG TATTCAGTAC 
AATGGAGCTG GTAGAAGAAC TGTGTTCAGT AGTCTTCAGT ATCCGTTTGG TTTGACTCAT 
GATGAAGAAC AAAAATTCTA TTGGACTGAT TGGAAAGACA ACCGCATTCA TTCAGTTGGT 
GTCTATGGAG AAGGATACCG TAGCTTCCAA ATTTCCCTTG GTGGATCAGG AAAAGTATTT 
GGAATCCTTG CCGTTCCAAA ATCATGCGTC GGTCCATCAA CTCCATGTTC TGAAGACAAC 
GGTGGCTGCC AACATCTTTG CCTTCCAGGA CAGAATGGTG CAGTTTGCGA ATGTCCGGAC 
AATGTCAAAG TGAAGGGATG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nid-1 ( NM_001269537.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256466.1 Caenorhabditis elegans NIDogen (basement membrane protein) (nid-1), mRNA.

Price & Availability↑

CloneID	OCa346023
Clone ID Related Accession (Same CDS sequence)	NM_001269537.1 , NM_001269537.2
Accession Version	NM_001269537.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	NIDogen (basement membrane protein)
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001269537.1.

ATGAAACACA AAAAAATGTT GATATTACTA TTTCTATATC TGTTGATAGA AACAACGACA 
GCAAACCCTG CATTCTACCC GTATGGACAA CCGGCTGGAG ATAATAGACT TGACTATTCT 
ATGGAACAAA GTGCAATTGC TCTTAAAATC CCACTGACAT ATATGGAACA GTCCGTTGAT 
GAGCTCTTTC TCTCACACAA TGGAATTGTC GGTCTTGGAA AACCGACGCC GGAGAAGATT 
GGAGGCCTAA TGACAATCTC TACACCTGCC ATAGCAGTTT TCTACGTGCC GGTTACAAGT 
GGAGTAATTG ATTATAGAGT TAGCTCTGAT GATCAGGGAC TGTTGACAAA ACTGACGCAA 
GATGTGAAAC AAGTTTTTGC GGATGCTGTT GAGTTCCATG GATTGCAAGC AGTAATCATC 
ACGTGGACAC AAATTGAGAA TGCTGAGAAA GATGGGCCTG CTAGTTTCCA GCTGGCAATT 
GTGTCAGACG GAATTTCAAC GTATGCAATT TTCCGTTATG AATCACTCCC CTGGTCATCT 
TCAATGGGAT ACTACGCACA GGCGGGATTC GTCCGCTCCA TTGGAAAAAT TCAGACAAAT 
GTGAATTCCG GAGGACCGGA TGTTAAGGAA CTTGTAAATT TGTCCAATAA TCAGTTCGGT 
AACTTTTTCA TCTTCCGAGT TTCGGGATCA GCAATTGAAG ATCCAAAGGA TGAAGGAGCT 
GATGATTATG ATTACAACAA TTACGATCAG GAATACGAGG GCGAACATCC AGCCGATTGC 
CCAAAAGACA AATTTGTTGA CAACTGCCCA GCCGAGTGTA GAACTGTAAG AGATGAACGA 
GGATGTGAAA GATGTATTTG TGCAGAGAGA CCACATGCCC CAAGAGCTCC TCAAGAGCCA 
AGACCAGAAC TGGAGAACAC TGTTGAAGAC GATGAAGATG ATGAACAAGT TGATGGAACA 
AGATATGAAG AGCCAGCTAG ACAGGCTCAG GAGCAACATC ACCAGCAACA CCATCAGCCG 
GATCAACCTC AGCAGCAGCC GCAACGCAGT CAGGAAGGAT CATTACCGAA AATGTCGTGC 
TCTCAAAGAG ATGACAAGAG CTGTCATGCA AACTCGGTTT GTCAAGATTT CGAAGGCGGA 
TTCTGCTGTA ACTGTGACAC GGGATTCTAT GGAAACGGAA AAGAGTGTCT TCCAAAAGGT 
GAACCACAAA GAATTTCTGG AAGCTTCGAG GGAGTCATCA ACAGAATTCC AATTGACAAG 
ACTGAACTTC ACACTTTTGC AACTTCGACT GATGGAAATG TTCACACAGC TGTTTCAAAG 
ATTCCATCGG ATCTTGGACA TCCACTTCGT TTCCTTTACT CAATTGGAGG AGTCATGGGA 
TGGCTATTTG CCGACGTTCA ATCCCCGAAT GTCTACAATG GATTCCAACT GACCGGTGGA 
CTTTTCAATA GAACCGTTGC ACTTCATATT GAACAAAACT ATTACGTAAC GATTAAGCAA 
GAGTTCTCCG GAAGAAACAT TCATGATTAC TTCAAATCTC ATTTATTTGT ATCGGGAACT 
CTTCCTGATA TTGCTCCAGG ATCTGAAGTT ATCTTCCCAG ATTATGAAGA AGAATACGTT 
AGAGAAAGAC GAGGATACTT AACATCAAAA GCAGCTTTCG ATGTAATCGT TAGAGATGGA 
GGAAATGTTC AAACATATCG AATGAGTGTT GATCAACAAA TTACTTTTGA AGAATGCCCA 
AATAAAGAGT TTGATCGTGA TCATTCAATG AAGCTTCATG TGAAGAGAAT TAATGTTGTC 
TATAACGATG ACGAAGGAGT CGTCCGATAT GGAGCAAAAA ACTTTGCAAC TCGATCAGTT 
GGGCCTGCTG TTTCAGCTCC TTCCGGTGGG CATTTCGATC GTCGCCAACA CGGACAATCC 
AACATGGCTT CAGAAAGACC AATTGAAATT CCAAGCCAAA GTGAATCAAT CAGTACTGAC 
TCAGTTTGTG CTCCAGGAAG ACATCAATGT ACTCTTCCAA ATATGAAGTG CCGTGTCGTT 
GATCCATCAT ACCGTTGTGA ATGTGAACCT GGATATCAAG CAGCTCATGA TGCATCCTCT 
CATATTGGAT GGATCTGTCA AGATTTGGAT GAATGTCAAC GAGGAGATCA TAATTGTGAC 
CAGCATGCCA AGTGTACTAA TCGACCAGGA TCTTTCTCAT GCCAATGTCT TCAAGGATAT 
CAAGGAGATG GAAGATCTTG TATTCGGGAG CATCAAGCAT CTCATCACGA ACATAATCAA 
CAGACTCCAC AAGAAATGGC CGGTGTCGGA GCGACAACTG AAGGATTCTG CACTGCACAT 
AACCAATGCC ATCAATGGGG AGAATGTGTA TTCACTTCTG AGCATCCAAC CGGAAGATGC 
AAGTGTAGGG GATGGTATGT TGGAGATGGA GTTAATCATT GTGGACCACC AGAGGAGAAC 
ATGCCAAAAC ACAATGCAAA TATTCCGCAG AGAGGAGGCC AAGCTTGTGG AAGTTATGTA 
TGCGATGTGA ATGCTGAGTG TATGCCAGAA CCAAGTGGAG GATCTGAATG TGTATGCAAG 
GCCGGATTCT CAGGAAATGG AGTAACTTGT GAAAGTCTGT TGGATGATCG TCATGCTCAT 
TCATCTCATA ATCGACATGA ACAACAACAA CAAACTGGAT CTCTTGGAAA AGTGTGCCGC 
TCTCACGATG AATGCTCTGA GCACGGAAGC TGCGCTTATC ACCACTCACT CGGTTATTAT 
CAATGCGCAT GTACCGAGCC ATATGTTGGC AATGGAGTCG AATGTACACT TCCAGGATCT 
TCTGCTTCAG TTCCTCAGCT GCCAAGTGAG CCGGCCGTTT TATCAACAGC GTCATGTAAT 
CCAAACTGTG GACCTGATGC TCAATGTGTT TATGATGACC ATAATAGACA ATATCGATGT 
GAATGTTATG CTGCATTCAT GGGAGATGGC TACAACTGTG TCCCCCTTGC AAAACCAAAT 
ATGGTACCTG CACAACCAAA AACATGCGTC GAATCATCTG ATTGTCATAT CAATGGACAC 
TGCGTTATCA ATGAACATGG AGCTGGAGAA TACATTTGTC AATGTCTTCC TGGATTCAGT 
GGAGACGGAT TCATCAACTG TCGCGGAGCT GATCAATGTA ACCCATCAAA CCCATCAGCT 
TGTTATCAGA ATGCTCATTG TGTTTACGAT GCGATTCTGA ATGCTCATGC CTGTAAATGT 
GTTGATGGAT TCAAGGGAGA TGGCACCAGT TGTGTTCCAT ATGCACCTGC AACAAACTGC 
AATTTGGAGC CAAGAATTTG TCATGCAAAT GCACAATGCG TGATGCACCA TGATACAAAT 
GCTTATGAGT GCATCTGTAA ACCAGGATCA TCTGGAGACG GTTACACTAA ATGTGATGTC 
ATCGAAACTC CTCGTTGCAC CAACTGCTCA ATCCATGCAT ATTGTGCTCA AAATCCGACT 
AGCGGTGCCT ATCAATGTAA ATGTAACGCA GGATATAATG GAAATGGACA TTTATGTGTC 
TCGATGAGCT CCTGCCTCGA TGACCGATCC CTCTGTGATG AGAATGCCGA TTGTGTTCCG 
GGAGAAGCTG GGCATTACGT CTGCAACTGC CACTATGGAT ACCATGGAGA TGGTAGATCA 
TGTTCCCCAG AATCCTCTAC TAGGAGCGAC AAGTTGCTAG TCGCCCGTGG AATGGCCATA 
TTTGAACGCT CAACAAATCC AGACGAGTAC GGAAAACAGC TGATTGTGAT CCCTCATCAT 
ATCCCAGTTG GCATTGACTT TGATTGTAAA GAAGAAAAAA TTGTGTGGAG TGACATGTCT 
GGTCACTCAA TTCGCACATC TTCATTAAAT GGAACTGAAC ATAAATCATA CTTCAATAAA 
GAACTCAGCA GTCCTGAAGG AATTGCCGTT GACTGGTCTT CAAGAAACGT GTACTATGCT 
GACTCAATGA ATGATGAAAT TGGAGTTGCT TCACTAAATG GAAAATTCAA GAAATCTCTT 
GTAACTGAAG GTCTTGTCAA TCCACGTTCA GTGGTTTTGG ATCTATACGG ACGACACTTG 
TACTACTCGG ATTGGCATCG TGAGAATCCA TACATCGGAA GAGTTGACAT GGATGGAAAG 
AACAATCGAG TATTCTTAAA TGAAGATGTC CATCTTCCAA ATGGTCTCAC TATTCTTCCA 
AACAGACGCG AACTCTGTTG GGTGGACGCT GGAAATCACA GACTTTCATG TATTCAGTAC 
AATGGAGCTG GTAGAAGAAC TGTGTTCAGT AGTCTTCAGT ATCCGTTTGG TTTGACTCAT 
GATGAAGAAC AAAAATTCTA TTGGACTGAT TGGAAAGACA ACCGCATTCA TTCAGTTGGT 
GTCTATGGAG AAGGATACCG TAGCTTCCAA ATTTCCCTTG GTGGATCAGG AAAAGTATTT 
GGAATCCTTG CCGTTCCAAA ATCATGCGTC GGTCCATCAA CTCCATGTTC TGAAGACAAC 
GGTGGCTGCC AACATCTTTG CCTTCCAGGA CAGAATGGTG CAGTTTGCGA ATGTCCGGAC 
AATGTCAAAG TGAAGGGATG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001256466.1
CDS	6..4589
Translation	MKHKKMLILLFLYLLIETTTANPAFYPYGQPAGDNRLDYSMEQSAIALKIPLTYMEQSVDELFLSHNGIVGLGKPTPEKIGGLMTISTPAIAVFYVPVTSGVIDYRVSSDDQGLLTKLTQDVKQVFADAVEFHGLQAVIITWTQIENAEKDGPASFQLAIVSDGISTYAIFRYESLPWSSSMGYYAQAGFVRSIGKIQTNVNSGGPDVKELVNLSNNQFGNFFIFRVSGSAIEDPKDEGADDYDYNNYDQEYEGEHPADCPKDKFVDNCPAECRTVRDERGCERCICAERPHAPRAPQEPRPELENTVEDDEDDEQVDGTRYEEPARQAQEQHHQQHHQPDQPQQQPQRSQEGSLPKMSCSQRDDKSCHANSVCQDFEGGFCCNCDTGFYGNGKECLPKGEPQRISGSFEGVINRIPIDKTELHTFATSTDGNVHTAVSKIPSDLGHPLRFLYSIGGVMGWLFADVQSPNVYNGFQLTGGLFNRTVALHIEQNYYVTIKQEFSGRNIHDYFKSHLFVSGTLPDIAPGSEVIFPDYEEEYVRERRGYLTSKAAFDVIVRDGGNVQTYRMSVDQQITFEECPNKEFDRDHSMKLHVKRINVVYNDDEGVVRYGAKNFATRSVGPAVSAPSGGHFDRRQHGQSNMASERPIEIPSQSESISTDSVCAPGRHQCTLPNMKCRVVDPSYRCECEPGYQAAHDASSHIGWICQDLDECQRGDHNCDQHAKCTNRPGSFSCQCLQGYQGDGRSCIREHQASHHEHNQQTPQEMAGVGATTEGFCTAHNQCHQWGECVFTSEHPTGRCKCRGWYVGDGVNHCGPPEENMPKHNANIPQRGGQACGSYVCDVNAECMPEPSGGSECVCKAGFSGNGVTCESLLDDRHAHSSHNRHEQQQQTGSLGKVCRSHDECSEHGSCAYHHSLGYYQCACTEPYVGNGVECTLPGSSASVPQLPSEPAVLSTASCNPNCGPDAQCVYDDHNRQYRCECYAAFMGDGYNCVPLAKPNMVPAQPKTCVESSDCHINGHCVINEHGAGEYICQCLPGFSGDGFINCRGADQCNPSNPSACYQNAHCVYDAILNAHACKCVDGFKGDGTSCVPYAPATNCNLEPRICHANAQCVMHHDTNAYECICKPGSSGDGYTKCDVIETPRCTNCSIHAYCAQNPTSGAYQCKCNAGYNGNGHLCVSMSSCLDDRSLCDENADCVPGEAGHYVCNCHYGYHGDGRSCSPESSTRSDKLLVARGMAIFERSTNPDEYGKQLIVIPHHIPVGIDFDCKEEKIVWSDMSGHSIRTSSLNGTEHKSYFNKELSSPEGIAVDWSSRNVYYADSMNDEIGVASLNGKFKKSLVTEGLVNPRSVVLDLYGRHLYYSDWHRENPYIGRVDMDGKNNRVFLNEDVHLPNGLTILPNRRELCWVDAGNHRLSCIQYNGAGRRTVFSSLQYPFGLTHDEEQKFYWTDWKDNRIHSVGVYGEGYRSFQISLGGSGKVFGILAVPKSCVGPSTPCSEDNGGCQHLCLPGQNGAVCECPDNVKVKGC

Target ORF information:

RefSeq Version	NM_001269537.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans NIDogen (basement membrane protein) (nid-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001269537.2

ORF Insert Sequence:

ATGAAACACA AAAAAATGTT GATATTACTA TTTCTATATC TGTTGATAGA AACAACGACA 
GCAAACCCTG CATTCTACCC GTATGGACAA CCGGCTGGAG ATAATAGACT TGACTATTCT 
ATGGAACAAA GTGCAATTGC TCTTAAAATC CCACTGACAT ATATGGAACA GTCCGTTGAT 
GAGCTCTTTC TCTCACACAA TGGAATTGTC GGTCTTGGAA AACCGACGCC GGAGAAGATT 
GGAGGCCTAA TGACAATCTC TACACCTGCC ATAGCAGTTT TCTACGTGCC GGTTACAAGT 
GGAGTAATTG ATTATAGAGT TAGCTCTGAT GATCAGGGAC TGTTGACAAA ACTGACGCAA 
GATGTGAAAC AAGTTTTTGC GGATGCTGTT GAGTTCCATG GATTGCAAGC AGTAATCATC 
ACGTGGACAC AAATTGAGAA TGCTGAGAAA GATGGGCCTG CTAGTTTCCA GCTGGCAATT 
GTGTCAGACG GAATTTCAAC GTATGCAATT TTCCGTTATG AATCACTCCC CTGGTCATCT 
TCAATGGGAT ACTACGCACA GGCGGGATTC GTCCGCTCCA TTGGAAAAAT TCAGACAAAT 
GTGAATTCCG GAGGACCGGA TGTTAAGGAA CTTGTAAATT TGTCCAATAA TCAGTTCGGT 
AACTTTTTCA TCTTCCGAGT TTCGGGATCA GCAATTGAAG ATCCAAAGGA TGAAGGAGCT 
GATGATTATG ATTACAACAA TTACGATCAG GAATACGAGG GCGAACATCC AGCCGATTGC 
CCAAAAGACA AATTTGTTGA CAACTGCCCA GCCGAGTGTA GAACTGTAAG AGATGAACGA 
GGATGTGAAA GATGTATTTG TGCAGAGAGA CCACATGCCC CAAGAGCTCC TCAAGAGCCA 
AGACCAGAAC TGGAGAACAC TGTTGAAGAC GATGAAGATG ATGAACAAGT TGATGGAACA 
AGATATGAAG AGCCAGCTAG ACAGGCTCAG GAGCAACATC ACCAGCAACA CCATCAGCCG 
GATCAACCTC AGCAGCAGCC GCAACGCAGT CAGGAAGGAT CATTACCGAA AATGTCGTGC 
TCTCAAAGAG ATGACAAGAG CTGTCATGCA AACTCGGTTT GTCAAGATTT CGAAGGCGGA 
TTCTGCTGTA ACTGTGACAC GGGATTCTAT GGAAACGGAA AAGAGTGTCT TCCAAAAGGT 
GAACCACAAA GAATTTCTGG AAGCTTCGAG GGAGTCATCA ACAGAATTCC AATTGACAAG 
ACTGAACTTC ACACTTTTGC AACTTCGACT GATGGAAATG TTCACACAGC TGTTTCAAAG 
ATTCCATCGG ATCTTGGACA TCCACTTCGT TTCCTTTACT CAATTGGAGG AGTCATGGGA 
TGGCTATTTG CCGACGTTCA ATCCCCGAAT GTCTACAATG GATTCCAACT GACCGGTGGA 
CTTTTCAATA GAACCGTTGC ACTTCATATT GAACAAAACT ATTACGTAAC GATTAAGCAA 
GAGTTCTCCG GAAGAAACAT TCATGATTAC TTCAAATCTC ATTTATTTGT ATCGGGAACT 
CTTCCTGATA TTGCTCCAGG ATCTGAAGTT ATCTTCCCAG ATTATGAAGA AGAATACGTT 
AGAGAAAGAC GAGGATACTT AACATCAAAA GCAGCTTTCG ATGTAATCGT TAGAGATGGA 
GGAAATGTTC AAACATATCG AATGAGTGTT GATCAACAAA TTACTTTTGA AGAATGCCCA 
AATAAAGAGT TTGATCGTGA TCATTCAATG AAGCTTCATG TGAAGAGAAT TAATGTTGTC 
TATAACGATG ACGAAGGAGT CGTCCGATAT GGAGCAAAAA ACTTTGCAAC TCGATCAGTT 
GGGCCTGCTG TTTCAGCTCC TTCCGGTGGG CATTTCGATC GTCGCCAACA CGGACAATCC 
AACATGGCTT CAGAAAGACC AATTGAAATT CCAAGCCAAA GTGAATCAAT CAGTACTGAC 
TCAGTTTGTG CTCCAGGAAG ACATCAATGT ACTCTTCCAA ATATGAAGTG CCGTGTCGTT 
GATCCATCAT ACCGTTGTGA ATGTGAACCT GGATATCAAG CAGCTCATGA TGCATCCTCT 
CATATTGGAT GGATCTGTCA AGATTTGGAT GAATGTCAAC GAGGAGATCA TAATTGTGAC 
CAGCATGCCA AGTGTACTAA TCGACCAGGA TCTTTCTCAT GCCAATGTCT TCAAGGATAT 
CAAGGAGATG GAAGATCTTG TATTCGGGAG CATCAAGCAT CTCATCACGA ACATAATCAA 
CAGACTCCAC AAGAAATGGC CGGTGTCGGA GCGACAACTG AAGGATTCTG CACTGCACAT 
AACCAATGCC ATCAATGGGG AGAATGTGTA TTCACTTCTG AGCATCCAAC CGGAAGATGC 
AAGTGTAGGG GATGGTATGT TGGAGATGGA GTTAATCATT GTGGACCACC AGAGGAGAAC 
ATGCCAAAAC ACAATGCAAA TATTCCGCAG AGAGGAGGCC AAGCTTGTGG AAGTTATGTA 
TGCGATGTGA ATGCTGAGTG TATGCCAGAA CCAAGTGGAG GATCTGAATG TGTATGCAAG 
GCCGGATTCT CAGGAAATGG AGTAACTTGT GAAAGTCTGT TGGATGATCG TCATGCTCAT 
TCATCTCATA ATCGACATGA ACAACAACAA CAAACTGGAT CTCTTGGAAA AGTGTGCCGC 
TCTCACGATG AATGCTCTGA GCACGGAAGC TGCGCTTATC ACCACTCACT CGGTTATTAT 
CAATGCGCAT GTACCGAGCC ATATGTTGGC AATGGAGTCG AATGTACACT TCCAGGATCT 
TCTGCTTCAG TTCCTCAGCT GCCAAGTGAG CCGGCCGTTT TATCAACAGC GTCATGTAAT 
CCAAACTGTG GACCTGATGC TCAATGTGTT TATGATGACC ATAATAGACA ATATCGATGT 
GAATGTTATG CTGCATTCAT GGGAGATGGC TACAACTGTG TCCCCCTTGC AAAACCAAAT 
ATGGTACCTG CACAACCAAA AACATGCGTC GAATCATCTG ATTGTCATAT CAATGGACAC 
TGCGTTATCA ATGAACATGG AGCTGGAGAA TACATTTGTC AATGTCTTCC TGGATTCAGT 
GGAGACGGAT TCATCAACTG TCGCGGAGCT GATCAATGTA ACCCATCAAA CCCATCAGCT 
TGTTATCAGA ATGCTCATTG TGTTTACGAT GCGATTCTGA ATGCTCATGC CTGTAAATGT 
GTTGATGGAT TCAAGGGAGA TGGCACCAGT TGTGTTCCAT ATGCACCTGC AACAAACTGC 
AATTTGGAGC CAAGAATTTG TCATGCAAAT GCACAATGCG TGATGCACCA TGATACAAAT 
GCTTATGAGT GCATCTGTAA ACCAGGATCA TCTGGAGACG GTTACACTAA ATGTGATGTC 
ATCGAAACTC CTCGTTGCAC CAACTGCTCA ATCCATGCAT ATTGTGCTCA AAATCCGACT 
AGCGGTGCCT ATCAATGTAA ATGTAACGCA GGATATAATG GAAATGGACA TTTATGTGTC 
TCGATGAGCT CCTGCCTCGA TGACCGATCC CTCTGTGATG AGAATGCCGA TTGTGTTCCG 
GGAGAAGCTG GGCATTACGT CTGCAACTGC CACTATGGAT ACCATGGAGA TGGTAGATCA 
TGTTCCCCAG AATCCTCTAC TAGGAGCGAC AAGTTGCTAG TCGCCCGTGG AATGGCCATA 
TTTGAACGCT CAACAAATCC AGACGAGTAC GGAAAACAGC TGATTGTGAT CCCTCATCAT 
ATCCCAGTTG GCATTGACTT TGATTGTAAA GAAGAAAAAA TTGTGTGGAG TGACATGTCT 
GGTCACTCAA TTCGCACATC TTCATTAAAT GGAACTGAAC ATAAATCATA CTTCAATAAA 
GAACTCAGCA GTCCTGAAGG AATTGCCGTT GACTGGTCTT CAAGAAACGT GTACTATGCT 
GACTCAATGA ATGATGAAAT TGGAGTTGCT TCACTAAATG GAAAATTCAA GAAATCTCTT 
GTAACTGAAG GTCTTGTCAA TCCACGTTCA GTGGTTTTGG ATCTATACGG ACGACACTTG 
TACTACTCGG ATTGGCATCG TGAGAATCCA TACATCGGAA GAGTTGACAT GGATGGAAAG 
AACAATCGAG TATTCTTAAA TGAAGATGTC CATCTTCCAA ATGGTCTCAC TATTCTTCCA 
AACAGACGCG AACTCTGTTG GGTGGACGCT GGAAATCACA GACTTTCATG TATTCAGTAC 
AATGGAGCTG GTAGAAGAAC TGTGTTCAGT AGTCTTCAGT ATCCGTTTGG TTTGACTCAT 
GATGAAGAAC AAAAATTCTA TTGGACTGAT TGGAAAGACA ACCGCATTCA TTCAGTTGGT 
GTCTATGGAG AAGGATACCG TAGCTTCCAA ATTTCCCTTG GTGGATCAGG AAAAGTATTT 
GGAATCCTTG CCGTTCCAAA ATCATGCGTC GGTCCATCAA CTCCATGTTC TGAAGACAAC 
GGTGGCTGCC AACATCTTTG CCTTCCAGGA CAGAATGGTG CAGTTTGCGA ATGTCCGGAC 
AATGTCAAAG TGAAGGGATG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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nid-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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