TSPY cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	TSPY
Entrez Gene ID	281554
Full Name	testis specific protein, Y-linked 1
Synonyms	TSPY-M1,TSPY1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001244608.1	NP_001231537.1	testis-specific Y-encoded protein 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LOC785608	XP_002707949.2
Bos taurus (cattle)	LOC786811	XP_002694428.2
Bos taurus (cattle)	TSPY	NP_001231537.1
Bos taurus (cattle)	LOC100336402	XP_002694429.2
Bos taurus (cattle)	LOC786698	XP_002683906.2

Gene Ontology
Bibliography

Related articles in PubMed

Testis-specific protein Y-encoded copy number is correlated to its expression and the field fertility of Canadian Holstein bulls.
Hamilton CK, Verduzco-G?mez AR, Favetta LA, Blondin P, King WA
Sexual development : genetics, molecular biology, evolution, endocrinology, embryology, and pathology of sex determination and differentiation6(5)231-9(2012)

Copy number variation of testis-specific protein, Y-encoded (TSPY) in 14 different breeds of cattle (Bos taurus).
Hamilton CK, Favetta LA, Di Meo GP, Floriot S, Perucatti A, Peippo J, Kantanen J, Eggen A, Iannuzzi L, King WA
Sexual development : genetics, molecular biology, evolution, endocrinology, embryology, and pathology of sex determination and differentiation3(4)205-13(2009)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Organization and concerted evolution of the ampliconic Y-chromosomal TSPY genes from cattle.
Verkaar EL, Zijlstra C, van 't Veld EM, Boutaga K, van Boxtel DC, Lenstra JA
Genomics84(3)468-74(2004 Sep)

Organization and expression of bovine TSPY.
Vogel T, Dechend F, Manz E, Jung C, Jakubiczka S, Fehr S, Schmidtke J, Schnieders F
Mammalian genome : official journal of the International Mammalian Genome Society8(7)491-6(1997 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

observed a highly variable rTSPY CN in bulls over a short period of time. Our results suggest the importance of further long term studies of the dynamics of rTSPY CN variablility
Title: Highly dynamic temporal changes of TSPY gene copy number in aging bulls.

Based on the model of cattle-yak F and F hybrid bull sterility, the CNV of TSPY may serve as a potential risk factor for crossbred bull ( x ) infertility.
Title: A tremendous expansion of copy number in crossbred bulls ( ? ).

TSPY copy number might represent a potential marker of bull fertility.
Title: Testis-specific protein Y-encoded copy number is correlated to its expression and the field fertility of Canadian Holstein bulls.

TSPY copy number was analyzed using relative real-time polymerase chain reaction (PCR) of the DNA of 14 different cattle breeds.
Title: Copy number variation of testis-specific protein, Y-encoded (TSPY) in 14 different breeds of cattle (Bos taurus).

The following TSPY gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TSPY cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa44543	NM_001244608.1 Latest version!	Bos taurus testis specific protein, Y-linked 1 (TSPY), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TSPY ( NM_001244608.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001231537.1 Bos taurus testis specific protein, Y-linked 1 (TSPY), mRNA.

Price & Availability↑

CloneID	OBa44543
Clone ID Related Accession (Same CDS sequence)	NM_001244608.1
Accession Version	NM_001244608.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 954bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-02-08
Organism	Bos taurus(cattle)
Product	testis-specific Y-encoded protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAFC03062199.1 and EF432553.2. On or before Sep 22, 2011 this sequence version replaced XM_002705041.1, XM_002683678.1. Sequence Note: This RefSeq record was created from transcripts from different breeds and genomic sequence data because no single transcript from the same breed was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data.

ATGTCGCGTC CCTTCGCCTC TGCCCCAGCT CGGGGTCACC GCCAAGGCCA AGAGGAACGT 
GAGAGGCGCT CAGAGGAAGG CGGGAGCGTC CCGGGACCCC GCACCTTCCA AGTTGTGAGC 
CCAGTTGTCA CGCCAGGCCA AGAGGCCACC CGCTTCAGGG TGGAGGCGGT GGAGGAGGGC 
GAGGCCCTGG TGGACGGAGA CGTGGCGGGG ATCGGGCGGG AGTTCCAGCT GCTTGCGGAA 
GACATCATGG AGGAGGTGGA GGTTGTGGCG GATGAGGAGC AGGAACAGCG GCCCTCCCAG 
GAGCTAGAGG AGAAGACGGT GGAGGAGCAG GGCCAGGAAA GGCCCGGAGG CCCTTGTGAG 
CGCCAGGAGC TGGATGCCGT GCAGGCACTG GCCGCCCTGC AGGTAGAACT GAGCTCTGAG 
CGTGAGCAAA ACCGCAGGGC TTACGTTCAG TTCATGCGCA AGAACCATCA GAGGAGGAAG 
CGTCACTTGG CTCGGAGGAG CGCCATCATC CAGGGCATCC CTGGCTTCTG GGCCAAAGCT 
ATTATGAGCC ACCCTCAAGT CTCCGTCCTG ATCAGCGACC AAGATCAAGA CTTTCTCGGC 
TACATGATGG ACTTGAAGGT GCAGGTGCGG AGCCATCCGC CGTCCCGCTG CAAGCTGATC 
TTTTCCTTTC GGGACAACCC CTACTTCTTG AACTCGGTGA TCATTAAGGA GTATTACCTT 
GACATCACTG GGTACAGGGC ACGTCGATCC ACTCCAGTCC ACTGGTTCTG GGACTTTGAA 
CGGGGAGCCC CCAGCCGCAG GCTGGACACC AGGAGCCTTA ACTTTCTCAA CTGGCTGTCA 
GGCCACAACG GCCCAGAATC GAACAGGATT GCTGAGATCA TCAGCGAAGA CGTGTGGGAC 
GATCCCCTGA AGTACTACCT CAGGGAGGAA GGTTCGTCCA TGAGAGACAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001231537.1
CDS	1..954
Translation	MSRPFASAPARGHRQGQEERERRSEEGGSVPGPRTFQVVSPVVTPGQEATRFRVEAVEEGEALVDGDVAGIGREFQLLAEDIMEEVEVVADEEQEQRPSQELEEKTVEEQGQERPGGPCERQELDAVQALAALQVELSSEREQNRRAYVQFMRKNHQRRKRHLARRSAIIQGIPGFWAKAIMSHPQVSVLISDQDQDFLGYMMDLKVQVRSHPPSRCKLIFSFRDNPYFLNSVIIKEYYLDITGYRARRSTPVHWFWDFERGAPSRRLDTRSLNFLNWLSGHNGPESNRIAEIISEDVWDDPLKYYLREEGSSMRDN

Target ORF information:

RefSeq Version	NM_001244608.1
Organism	Bos taurus(cattle)
Definition	Bos taurus testis specific protein, Y-linked 1 (TSPY), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001244608.1

ORF Insert Sequence:

ATGTCGCGTC CCTTCGCCTC TGCCCCAGCT CGGGGTCACC GCCAAGGCCA AGAGGAACGT 
GAGAGGCGCT CAGAGGAAGG CGGGAGCGTC CCGGGACCCC GCACCTTCCA AGTTGTGAGC 
CCAGTTGTCA CGCCAGGCCA AGAGGCCACC CGCTTCAGGG TGGAGGCGGT GGAGGAGGGC 
GAGGCCCTGG TGGACGGAGA CGTGGCGGGG ATCGGGCGGG AGTTCCAGCT GCTTGCGGAA 
GACATCATGG AGGAGGTGGA GGTTGTGGCG GATGAGGAGC AGGAACAGCG GCCCTCCCAG 
GAGCTAGAGG AGAAGACGGT GGAGGAGCAG GGCCAGGAAA GGCCCGGAGG CCCTTGTGAG 
CGCCAGGAGC TGGATGCCGT GCAGGCACTG GCCGCCCTGC AGGTAGAACT GAGCTCTGAG 
CGTGAGCAAA ACCGCAGGGC TTACGTTCAG TTCATGCGCA AGAACCATCA GAGGAGGAAG 
CGTCACTTGG CTCGGAGGAG CGCCATCATC CAGGGCATCC CTGGCTTCTG GGCCAAAGCT 
ATTATGAGCC ACCCTCAAGT CTCCGTCCTG ATCAGCGACC AAGATCAAGA CTTTCTCGGC 
TACATGATGG ACTTGAAGGT GCAGGTGCGG AGCCATCCGC CGTCCCGCTG CAAGCTGATC 
TTTTCCTTTC GGGACAACCC CTACTTCTTG AACTCGGTGA TCATTAAGGA GTATTACCTT 
GACATCACTG GGTACAGGGC ACGTCGATCC ACTCCAGTCC ACTGGTTCTG GGACTTTGAA 
CGGGGAGCCC CCAGCCGCAG GCTGGACACC AGGAGCCTTA ACTTTCTCAA CTGGCTGTCA 
GGCCACAACG GCCCAGAATC GAACAGGATT GCTGAGATCA TCAGCGAAGA CGTGTGGGAC 
GATCCCCTGA AGTACTACCT CAGGGAGGAA GGTTCGTCCA TGAGAGACAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Testis-specific protein Y-encoded copy number is correlated to its expression and the field fertility of Canadian Holstein bulls.
Sexual development : genetics, molecular biology, evolution, endocrinology, embryology, and pathology of sex determination and differentiation6(5)231-9(2012)
Hamilton CK,Verduzco-G?mez AR,Favetta LA,Blondin P,King WA

Copy number variation of testis-specific protein, Y-encoded (TSPY) in 14 different breeds of cattle (Bos taurus).
Sexual development : genetics, molecular biology, evolution, endocrinology, embryology, and pathology of sex determination and differentiation3(4)205-13(2009)
Hamilton CK,Favetta LA,Di Meo GP,Floriot S,Perucatti A,Peippo J,Kantanen J,Eggen A,Iannuzzi L,King WA

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Organization and concerted evolution of the ampliconic Y-chromosomal TSPY genes from cattle.
Genomics84(3)468-74(2004 Sep)
Verkaar EL,Zijlstra C,van 't Veld EM,Boutaga K,van Boxtel DC,Lenstra JA

Organization and expression of bovine TSPY.
Mammalian genome : official journal of the International Mammalian Genome Society8(7)491-6(1997 Jul)
Vogel T,Dechend F,Manz E,Jung C,Jakubiczka S,Fehr S,Schmidtke J,Schnieders F

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TSPY cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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