MICAL1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	MICAL1
Entrez Gene ID	568573
Full Name	microtubule associated monooxygenase, calponin and LIM domain containing 1
Synonyms	MICAL-1,zgc:171928
General protein information	Preferred Names microtubule associated monooxygenase, calponin and LIM domain containing 1 Names [F-actin]-monooxygenase mical1 F-actin-methionine sulfoxide oxidase mical1 [F-actin]-methionine sulfoxide oxidase mical1 microtubule associated monoxygenase, calponin and LIM domain containing 1 molecule interacting with CasL 1 molecule interacting with CasL protein 1 protein-methionine sulfoxide oxidase mical1
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003201226.5	XP_003201274.1	F-actin-methionine sulfoxide oxidase mical1 isoform X1
NM_001316730.1	NP_001303659.1	[F-actin]-monooxygenase mical1
XM_009297077.2	XP_009295352.1	protein-methionine sulfoxide oxidase mical1 isoform X1
XM_017353592.1	XP_017209081.1	protein-methionine sulfoxide oxidase mical1 isoform X2
XM_003201226.4	XP_003201274.1	protein-methionine sulfoxide oxidase mical1 isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

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Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Identification and expression analysis of mical family genes in zebrafish.
Xue Y, Kuok C, Xiao A, Zhu Z, Lin S, Zhang B
Journal of genetics and genomics = Yi chuan xue bao37(10)685-93(2010 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MICAL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MICAL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa57390	XM_017353592.1 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 1 (mical1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
ODa28049	NM_001316730.1 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 1 (mical1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa28049	XM_003201226.5 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 1 (mical1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa28049	XM_009297077.2 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 1 (mical1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
ODa28049	XM_003201226.4	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 1 (mical1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MICAL1 ( XM_017353592.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017209081.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 1 (mical1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa57390
Clone ID Related Accession (Same CDS sequence)	XM_017353592.1
Accession Version	XM_017353592.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 918bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein-methionine sulfoxide oxidase mical1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAACC CGCTGGACTC AGTCAACCCC AGCCATGCTC TGTTTGAGGG CTTTGTCCAG 
GCGCAGACAT GTAAAGAGAC GCAGCAAAAC TTCACCGAGC TCTGCCGCCA CCTGCAGGTC 
GACCCCAAAG ACTACAAGCA CTTCTACAGC AAACTGAAGG ACAGACTGAA CTACTGGAAG 
GCCAAAGACC TCTGGCAGAA GATCGACAAG AGAGCCGCTC ATCCCGACTA TGACCAGGGA 
AAAGCATGTC ACCAAAACAA GTGTTTGGTG TTGGGCGCGG GTCCCTGTGG TCTCAGGACA 
GCCATTGAAT TGGCTCTGCT TGGCGCTCAG GTTGTTGTTC TGGAGAAAAG ATCATCTTTC 
ACGAGGAATA ATGTCCTGCA TTTGTGGCCC TTCACCATTA AAGACCTTCT CAACCTCGGG 
GCAAAGAAAT TTTATGGCCG ATTCTGCAGT GGCTCCATCC ATCACATAAG TATCCGTCAG 
CTGCAGTTGA TTCTGCTGAA AGTGGCTCTG TTTTTGGGGG TGGAGGTTCA CACTGGTGTG 
GCTTTTGAAG GTCTAAATGA ACCATCAGGA TCTGCAGGAT GGAGAGCAAA TGTGTCTCCA 
AAGTCCCACC CAGTTGCAGA TTTTCAGTTT GATGTGTTTA TTTCAGCCGG AGGGGGGAAA 
TATGTGCCTG ATGGTTTTAA GATAAAGGAG CTCCGAGGAA AGCTGGCTAT TGGTATCACT 
GCAAACTTTG TAAACCGTCA CACTAAACAA GAGGCTCAGG TGCAGGAGAT CAGCGGTGTG 
GCCCGAATTT ACAACCAGCA GTTTTTCCAG GCTCTTCAGT CTGAGATTGG TAGAGACTCA 
TACAAACATT TATCTTCTCT AAACGAGTGC CAGTTCTTAA CTCCCATTAT GCCTAATAAG 
CTGCATTTGG TCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209081.1
CDS	125..1042
Translation	MVNPLDSVNPSHALFEGFVQAQTCKETQQNFTELCRHLQVDPKDYKHFYSKLKDRLNYWKAKDLWQKIDKRAAHPDYDQGKACHQNKCLVLGAGPCGLRTAIELALLGAQVVVLEKRSSFTRNNVLHLWPFTIKDLLNLGAKKFYGRFCSGSIHHISIRQLQLILLKVALFLGVEVHTGVAFEGLNEPSGSAGWRANVSPKSHPVADFQFDVFISAGGGKYVPDGFKIKELRGKLAIGITANFVNRHTKQEAQVQEISGVARIYNQQFFQALQSEIGRDSYKHLSSLNECQFLTPIMPNKLHLVM

Target ORF information:

RefSeq Version	XM_017353592.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 1 (mical1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353592.1

ORF Insert Sequence:

ATGGTCAACC CGCTGGACTC AGTCAACCCC AGCCATGCTC TGTTTGAGGG CTTTGTCCAG 
GCGCAGACAT GTAAAGAGAC GCAGCAAAAC TTCACCGAGC TCTGCCGCCA CCTGCAGGTC 
GACCCCAAAG ACTACAAGCA CTTCTACAGC AAACTGAAGG ACAGACTGAA CTACTGGAAG 
GCCAAAGACC TCTGGCAGAA GATCGACAAG AGAGCCGCTC ATCCCGACTA TGACCAGGGA 
AAAGCATGTC ACCAAAACAA GTGTTTGGTG TTGGGCGCGG GTCCCTGTGG TCTCAGGACA 
GCCATTGAAT TGGCTCTGCT TGGCGCTCAG GTTGTTGTTC TGGAGAAAAG ATCATCTTTC 
ACGAGGAATA ATGTCCTGCA TTTGTGGCCC TTCACCATTA AAGACCTTCT CAACCTCGGG 
GCAAAGAAAT TTTATGGCCG ATTCTGCAGT GGCTCCATCC ATCACATAAG TATCCGTCAG 
CTGCAGTTGA TTCTGCTGAA AGTGGCTCTG TTTTTGGGGG TGGAGGTTCA CACTGGTGTG 
GCTTTTGAAG GTCTAAATGA ACCATCAGGA TCTGCAGGAT GGAGAGCAAA TGTGTCTCCA 
AAGTCCCACC CAGTTGCAGA TTTTCAGTTT GATGTGTTTA TTTCAGCCGG AGGGGGGAAA 
TATGTGCCTG ATGGTTTTAA GATAAAGGAG CTCCGAGGAA AGCTGGCTAT TGGTATCACT 
GCAAACTTTG TAAACCGTCA CACTAAACAA GAGGCTCAGG TGCAGGAGAT CAGCGGTGTG 
GCCCGAATTT ACAACCAGCA GTTTTTCCAG GCTCTTCAGT CTGAGATTGG TAGAGACTCA 
TACAAACATT TATCTTCTCT AAACGAGTGC CAGTTCTTAA CTCCCATTAT GCCTAATAAG 
CTGCATTTGG TCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL1 ( NM_001316730.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001303659.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 1 (mical1), mRNA.

Price & Availability↑

CloneID	ODa28049
Clone ID Related Accession (Same CDS sequence)	XM_003201226.5 , NM_001316730.1 , XM_009297077.2 , XM_003201226.4
Accession Version	NM_001316730.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	[F-actin]-monooxygenase mical1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX548066.3, HM151012.1 and HM151004.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## CDS exon combination :: GFIL01026449.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA898400 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGGTCAACC CGCTGGACTC AGTCAACCCC AGCCATGCTC TGTTTGAGGG CTTTGTCCAG 
GCGCAGACAT GTAAAGAGAC GCAGCAAAAC TTCACCGAGC TCTGCCGCCA CCTGCAGGTC 
GACCCCAAAG ACTACAAGCA CTTCTACAGC AAACTGAAGG ACAGACTGAA CTACTGGAAG 
GCCAAAGACC TCTGGCAGAA GATCGACAAG AGAGCCGCTC ATCCCGACTA TGACCAGGGA 
AAAGCATGTC ACCAAAACAA GTGTTTGGTG TTGGGCGCGG GTCCCTGTGG TCTCAGGACA 
GCCATTGAAT TGGCTCTGCT TGGCGCTCAG GTTGTTGTTC TGGAGAAAAG ATCATCTTTC 
ACGAGGAATA ATGTCCTGCA TTTGTGGCCC TTCACCATTA AAGACCTTCT CAACCTCGGG 
GCAAAGAAAT TTTATGGCCG ATTCTGCAGT GGCTCCATCC ATCACATAAG TATCCGTCAG 
CTGCAGTTGA TTCTGCTGAA AGTGGCTCTG TTTTTGGGGG TGGAGGTTCA CACTGGTGTG 
GCTTTTGAAG GTCTAAATGA ACCATCAGGA TCTGCAGGAT GGAGAGCAAA TGTGTCTCCA 
AAGTCCCACC CAGTTGCAGA TTTTCAGTTT GATGTGTTTA TTTCAGCCGG AGGGGGGAAA 
TATGTGCCTG ATGGTTTTAA GATAAAGGAG CTCCGAGGAA AGCTGGCTAT TGGTATCACT 
GCAAACTTTG TAAACCGTCA CACTAAACAA GAGGCTCAGG TGCAGGAGAT CAGCGGTGTG 
GCCCGAATTT ACAACCAGCA GTTTTTCCAG GCTCTTCAGT CTGAGATTGG CGTTGATCTA 
GAAAACATTG TCTACTACAA AGACGACACC CACTATTTTG TGATGACTGC AAAAAAGGCC 
AGCTTGTTAA AGAAAGGAGT CATAAAGCAG GATTTCTCTG ATGCCGATAA GCTCCTGGCT 
CCCTCAAATG TGAATCAGGA GGCACTGCAG GATTACGCAT TCGAAGCATG TGATTTCTCC 
ACAGAGCACT TGCTGCCCAA CCTGGAATTC GCCAAGAACC ACAAAGGCCA AGCGGACGTG 
GCCATGTTTG ACTTTACCTG CATGCAGAGG GCAGAGAGCG CCTCTCTGGT GAAGGAGAGG 
AATGGCAAGA GGCTGCTCAT CGGGCTGGTG GGAGATTCTC TGGTAGAGCC TTTTTGGCCT 
TTGGGCACTG GCATTGCTCG TGGTTTTCTG GGTGCGTTTG ATGCAGCGTG GATGGTCAGG 
AGTTGGGGAA AAGGTGTGCA GCCCATGGAG GTTCTGGCAG AGAGGGAAAG TGTGTATCAG 
TTGCTTTCTC AAACAACTCC AGAAAACACC AGCAAGAACT ACATGGCCTA CAGCATTGAT 
CCAAGCACAC GATACCCGAA CATCAACCTG TCCTCCATCA AACCCAGACA GGTCAAGCGT 
CTGTATGAAG CAGAAGAGCA GGAAAGCAAA CCAAACAAAC TGAAGAAACC TGACATAAAA 
GCTAAACCTA GAAAAGACTC TATGAAGCGT CTGGAGGAGC TGTTGTCCTG GTGTCAGAAG 
AATACAGTGG GATACGAGCA TGTGAAGGTC AAAGATCTGA GCGAGTCTTG GAGGTCTGGT 
CTGGCTCTCT GTGCTCTCAT CCACAGCTTC AGACCTGAGC TTGTTGACAT GTCGGCTCTG 
GATGAGTATA ATATCATCAA GAACAATAAG TTGGCTTTTG ATCTGATGGA GAAAGAGTTC 
GGCATCACCC CCATCATGCG GCCCGGCGAT ATGATGACCT GTGGTAAAAT CGACCAGCTG 
TCCATGGTGG TTTACCTCAC ACAGATACGC AACGCTCTTA CTGAGAAAGA CACACCAGCA 
GCGCAGTCCA ACACACTCTC CCTGTCCAGA AAGCGTTCAG CCGTTGCCTT CCTCAACACA 
CTCAAACGCA ACTCTCTGCA AAGACATAAG GATCGACTGG CATCCGTGAA AGGACCAAGA 
CAGCAAAACA TGAAGGAGAA AGAAGAGAAG AAGGATGTGA AAGAGGAAAG TCTCTCCTCA 
GAGACGAGTG CATGTGAACC CTGTTATTTC TGCAAGAAGC ATCTGTATGT GGTGGAGAGA 
GAAAGTGCAG AGGGAAAGTT CTTCCATCGC AGCTGCTTCA ACTGTTTTCA GTGCGGCTCC 
ACTCTGCGGC AGGGCGGATA CTCTTTTCAC TCTGACAATG GGAGGTTTTA CTGCGAGCTG 
CACTCACTGG CTGAGGAAGA GGAGGGTGAT GAAGGTCATG GAGGCGCACA GAATCATACT 
GAGAACGGAA GTAAGGAGGA CAAAAATGGA GAAACAACAG CGGCTTCATC CCCACCTGCG 
CATCTCTCAA TAAAGCGTAA GGGCTCGTAC AAGATCTCTG TTGACCCTGA CTTTGATGAA 
TCAACTGAGT TTCCTGCTCC AGACCAAGAT GAACCACCTG ATCTTGAGGA GTCCCATCAA 
CCTCCCAAAC CTTCAGAGCT CAGTGCGGAA AACACAAACA TGGAAAACCA ACAACACAAC 
ATCAATCCAG TTCCTGCCCC GCGTGGCTCC AGAGCTCCGC TCCCGAAACC TCGCACTGTC 
CATAATGTGG TGCACGAACC CTGTAATATT CCAGAGGAAG CAGAGCAGAT TCCTGAGGAG 
CCCAAACCCA AACCGTCCCT GCGCAAACTC CAGCAGAGCG AAGAGGAGAA GGTGGATCTG 
TTGAGCCAGG ATTCAGACTC TGAGACACGG GGCTCGTCTT CTGCCGCCTC CACCTCCTCC 
TCATCCAAAC AGCATGAAGA GGAGGGCTAC TGGAGCGGAG GCACGACGTG GGGGAAGAGC 
CATCGTGAGC AACGAAACCG CCCCTGCATA AGGAGGAAGA GTGAACCTCC GCTGCCGCTG 
ACTGGCCATT CTCAGCACGG AAAGATGCGC TCCAAGTTTT CCCCGTGGAA TCTGTCTTCA 
CCGCGGCTCC AGCAGCGATT CAGCGTTCAC AGAGTTCCTG CAGGTCAAAG TCAGCCAGAT 
CAGTATGTAT CAGAAGATGA TAATGAAGAT GATGAAGATG AGGATGAAGA GGACCTTCAG 
GCTGAACATT ATTTGGATTG CGAGGGAGCT GATTTTGAAT TTTCGGATTC AGAGAAACGC 
AACTTAAAGA GGATGAAAAC TCTGGAGCGG AAAGCAAAAA TGACTGAAAT CCAGCGTTTC 
CACAAAGCTC AGTCGATTCA GAGACGTCTG GAAGAGATCG AAGTGACCTT TAAAGAGCTG 
GAGGAGAAAG GAGTGGAGCT GGAGCGAGCT CTGAGAGGAG AGACGGGTAC AGGTGACCCT 
GAAATCATTG ATCAGTGGAT TGAACTGGTC CAGGAGAAAA ACAACCTGCT GTCAGAGGAG 
TCTGATCTCA TGGTGGCGTC TCGACAGCTG GAGCTGGAGG ACAAACAGAG CATGCTGGAG 
ATGGAGCTGC GCCGCTACAT GGAAATGGAT GATTCAGAGA AAAGCCCTGA GCAGCAAAAG 
CACGAGGCAG AGATCCTGCA GGAGATGCTG GACGTGGTAG ACATGAGGGA TTCACTGGTG 
GCCTTTCTGG AGGAGAAGAG GCTGAAGGAG GTCAATGATC AATTTAATTC AAGCCTGGAT 
GCCAAAAGAC GTTCAACCAC AGCCTCCCAG GTGCACTGGG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303659.1
CDS	35..3679
Translation	MVNPLDSVNPSHALFEGFVQAQTCKETQQNFTELCRHLQVDPKDYKHFYSKLKDRLNYWKAKDLWQKIDKRAAHPDYDQGKACHQNKCLVLGAGPCGLRTAIELALLGAQVVVLEKRSSFTRNNVLHLWPFTIKDLLNLGAKKFYGRFCSGSIHHISIRQLQLILLKVALFLGVEVHTGVAFEGLNEPSGSAGWRANVSPKSHPVADFQFDVFISAGGGKYVPDGFKIKELRGKLAIGITANFVNRHTKQEAQVQEISGVARIYNQQFFQALQSEIGVDLENIVYYKDDTHYFVMTAKKASLLKKGVIKQDFSDADKLLAPSNVNQEALQDYAFEACDFSTEHLLPNLEFAKNHKGQADVAMFDFTCMQRAESASLVKERNGKRLLIGLVGDSLVEPFWPLGTGIARGFLGAFDAAWMVRSWGKGVQPMEVLAERESVYQLLSQTTPENTSKNYMAYSIDPSTRYPNINLSSIKPRQVKRLYEAEEQESKPNKLKKPDIKAKPRKDSMKRLEELLSWCQKNTVGYEHVKVKDLSESWRSGLALCALIHSFRPELVDMSALDEYNIIKNNKLAFDLMEKEFGITPIMRPGDMMTCGKIDQLSMVVYLTQIRNALTEKDTPAAQSNTLSLSRKRSAVAFLNTLKRNSLQRHKDRLASVKGPRQQNMKEKEEKKDVKEESLSSETSACEPCYFCKKHLYVVERESAEGKFFHRSCFNCFQCGSTLRQGGYSFHSDNGRFYCELHSLAEEEEGDEGHGGAQNHTENGSKEDKNGETTAASSPPAHLSIKRKGSYKISVDPDFDESTEFPAPDQDEPPDLEESHQPPKPSELSAENTNMENQQHNINPVPAPRGSRAPLPKPRTVHNVVHEPCNIPEEAEQIPEEPKPKPSLRKLQQSEEEKVDLLSQDSDSETRGSSSAASTSSSSKQHEEEGYWSGGTTWGKSHREQRNRPCIRRKSEPPLPLTGHSQHGKMRSKFSPWNLSSPRLQQRFSVHRVPAGQSQPDQYVSEDDNEDDEDEDEEDLQAEHYLDCEGADFEFSDSEKRNLKRMKTLERKAKMTEIQRFHKAQSIQRRLEEIEVTFKELEEKGVELERALRGETGTGDPEIIDQWIELVQEKNNLLSEESDLMVASRQLELEDKQSMLEMELRRYMEMDDSEKSPEQQKHEAEILQEMLDVVDMRDSLVAFLEEKRLKEVNDQFNSSLDAKRRSTTASQVHWE

Target ORF information:

RefSeq Version	NM_001316730.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 1 (mical1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316730.1

ORF Insert Sequence:

ATGGTCAACC CGCTGGACTC AGTCAACCCC AGCCATGCTC TGTTTGAGGG CTTTGTCCAG 
GCGCAGACAT GTAAAGAGAC GCAGCAAAAC TTCACCGAGC TCTGCCGCCA CCTGCAGGTC 
GACCCCAAAG ACTACAAGCA CTTCTACAGC AAACTGAAGG ACAGACTGAA CTACTGGAAG 
GCCAAAGACC TCTGGCAGAA GATCGACAAG AGAGCCGCTC ATCCCGACTA TGACCAGGGA 
AAAGCATGTC ACCAAAACAA GTGTTTGGTG TTGGGCGCGG GTCCCTGTGG TCTCAGGACA 
GCCATTGAAT TGGCTCTGCT TGGCGCTCAG GTTGTTGTTC TGGAGAAAAG ATCATCTTTC 
ACGAGGAATA ATGTCCTGCA TTTGTGGCCC TTCACCATTA AAGACCTTCT CAACCTCGGG 
GCAAAGAAAT TTTATGGCCG ATTCTGCAGT GGCTCCATCC ATCACATAAG TATCCGTCAG 
CTGCAGTTGA TTCTGCTGAA AGTGGCTCTG TTTTTGGGGG TGGAGGTTCA CACTGGTGTG 
GCTTTTGAAG GTCTAAATGA ACCATCAGGA TCTGCAGGAT GGAGAGCAAA TGTGTCTCCA 
AAGTCCCACC CAGTTGCAGA TTTTCAGTTT GATGTGTTTA TTTCAGCCGG AGGGGGGAAA 
TATGTGCCTG ATGGTTTTAA GATAAAGGAG CTCCGAGGAA AGCTGGCTAT TGGTATCACT 
GCAAACTTTG TAAACCGTCA CACTAAACAA GAGGCTCAGG TGCAGGAGAT CAGCGGTGTG 
GCCCGAATTT ACAACCAGCA GTTTTTCCAG GCTCTTCAGT CTGAGATTGG CGTTGATCTA 
GAAAACATTG TCTACTACAA AGACGACACC CACTATTTTG TGATGACTGC AAAAAAGGCC 
AGCTTGTTAA AGAAAGGAGT CATAAAGCAG GATTTCTCTG ATGCCGATAA GCTCCTGGCT 
CCCTCAAATG TGAATCAGGA GGCACTGCAG GATTACGCAT TCGAAGCATG TGATTTCTCC 
ACAGAGCACT TGCTGCCCAA CCTGGAATTC GCCAAGAACC ACAAAGGCCA AGCGGACGTG 
GCCATGTTTG ACTTTACCTG CATGCAGAGG GCAGAGAGCG CCTCTCTGGT GAAGGAGAGG 
AATGGCAAGA GGCTGCTCAT CGGGCTGGTG GGAGATTCTC TGGTAGAGCC TTTTTGGCCT 
TTGGGCACTG GCATTGCTCG TGGTTTTCTG GGTGCGTTTG ATGCAGCGTG GATGGTCAGG 
AGTTGGGGAA AAGGTGTGCA GCCCATGGAG GTTCTGGCAG AGAGGGAAAG TGTGTATCAG 
TTGCTTTCTC AAACAACTCC AGAAAACACC AGCAAGAACT ACATGGCCTA CAGCATTGAT 
CCAAGCACAC GATACCCGAA CATCAACCTG TCCTCCATCA AACCCAGACA GGTCAAGCGT 
CTGTATGAAG CAGAAGAGCA GGAAAGCAAA CCAAACAAAC TGAAGAAACC TGACATAAAA 
GCTAAACCTA GAAAAGACTC TATGAAGCGT CTGGAGGAGC TGTTGTCCTG GTGTCAGAAG 
AATACAGTGG GATACGAGCA TGTGAAGGTC AAAGATCTGA GCGAGTCTTG GAGGTCTGGT 
CTGGCTCTCT GTGCTCTCAT CCACAGCTTC AGACCTGAGC TTGTTGACAT GTCGGCTCTG 
GATGAGTATA ATATCATCAA GAACAATAAG TTGGCTTTTG ATCTGATGGA GAAAGAGTTC 
GGCATCACCC CCATCATGCG GCCCGGCGAT ATGATGACCT GTGGTAAAAT CGACCAGCTG 
TCCATGGTGG TTTACCTCAC ACAGATACGC AACGCTCTTA CTGAGAAAGA CACACCAGCA 
GCGCAGTCCA ACACACTCTC CCTGTCCAGA AAGCGTTCAG CCGTTGCCTT CCTCAACACA 
CTCAAACGCA ACTCTCTGCA AAGACATAAG GATCGACTGG CATCCGTGAA AGGACCAAGA 
CAGCAAAACA TGAAGGAGAA AGAAGAGAAG AAGGATGTGA AAGAGGAAAG TCTCTCCTCA 
GAGACGAGTG CATGTGAACC CTGTTATTTC TGCAAGAAGC ATCTGTATGT GGTGGAGAGA 
GAAAGTGCAG AGGGAAAGTT CTTCCATCGC AGCTGCTTCA ACTGTTTTCA GTGCGGCTCC 
ACTCTGCGGC AGGGCGGATA CTCTTTTCAC TCTGACAATG GGAGGTTTTA CTGCGAGCTG 
CACTCACTGG CTGAGGAAGA GGAGGGTGAT GAAGGTCATG GAGGCGCACA GAATCATACT 
GAGAACGGAA GTAAGGAGGA CAAAAATGGA GAAACAACAG CGGCTTCATC CCCACCTGCG 
CATCTCTCAA TAAAGCGTAA GGGCTCGTAC AAGATCTCTG TTGACCCTGA CTTTGATGAA 
TCAACTGAGT TTCCTGCTCC AGACCAAGAT GAACCACCTG ATCTTGAGGA GTCCCATCAA 
CCTCCCAAAC CTTCAGAGCT CAGTGCGGAA AACACAAACA TGGAAAACCA ACAACACAAC 
ATCAATCCAG TTCCTGCCCC GCGTGGCTCC AGAGCTCCGC TCCCGAAACC TCGCACTGTC 
CATAATGTGG TGCACGAACC CTGTAATATT CCAGAGGAAG CAGAGCAGAT TCCTGAGGAG 
CCCAAACCCA AACCGTCCCT GCGCAAACTC CAGCAGAGCG AAGAGGAGAA GGTGGATCTG 
TTGAGCCAGG ATTCAGACTC TGAGACACGG GGCTCGTCTT CTGCCGCCTC CACCTCCTCC 
TCATCCAAAC AGCATGAAGA GGAGGGCTAC TGGAGCGGAG GCACGACGTG GGGGAAGAGC 
CATCGTGAGC AACGAAACCG CCCCTGCATA AGGAGGAAGA GTGAACCTCC GCTGCCGCTG 
ACTGGCCATT CTCAGCACGG AAAGATGCGC TCCAAGTTTT CCCCGTGGAA TCTGTCTTCA 
CCGCGGCTCC AGCAGCGATT CAGCGTTCAC AGAGTTCCTG CAGGTCAAAG TCAGCCAGAT 
CAGTATGTAT CAGAAGATGA TAATGAAGAT GATGAAGATG AGGATGAAGA GGACCTTCAG 
GCTGAACATT ATTTGGATTG CGAGGGAGCT GATTTTGAAT TTTCGGATTC AGAGAAACGC 
AACTTAAAGA GGATGAAAAC TCTGGAGCGG AAAGCAAAAA TGACTGAAAT CCAGCGTTTC 
CACAAAGCTC AGTCGATTCA GAGACGTCTG GAAGAGATCG AAGTGACCTT TAAAGAGCTG 
GAGGAGAAAG GAGTGGAGCT GGAGCGAGCT CTGAGAGGAG AGACGGGTAC AGGTGACCCT 
GAAATCATTG ATCAGTGGAT TGAACTGGTC CAGGAGAAAA ACAACCTGCT GTCAGAGGAG 
TCTGATCTCA TGGTGGCGTC TCGACAGCTG GAGCTGGAGG ACAAACAGAG CATGCTGGAG 
ATGGAGCTGC GCCGCTACAT GGAAATGGAT GATTCAGAGA AAAGCCCTGA GCAGCAAAAG 
CACGAGGCAG AGATCCTGCA GGAGATGCTG GACGTGGTAG ACATGAGGGA TTCACTGGTG 
GCCTTTCTGG AGGAGAAGAG GCTGAAGGAG GTCAATGATC AATTTAATTC AAGCCTGGAT 
GCCAAAAGAC GTTCAACCAC AGCCTCCCAG GTGCACTGGG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL1 ( XM_003201226.5 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_003201274.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 1 (mical1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa28049
Clone ID Related Accession (Same CDS sequence)	XM_003201226.5 , NM_001316730.1 , XM_009297077.2 , XM_003201226.4
Accession Version	XM_003201226.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	F-actin-methionine sulfoxide oxidase mical1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_003201226.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAACC CGCTGGACTC AGTCAACCCC AGCCATGCTC TGTTTGAGGG CTTTGTCCAG 
GCGCAGACAT GTAAAGAGAC GCAGCAAAAC TTCACCGAGC TCTGCCGCCA CCTGCAGGTC 
GACCCCAAAG ACTACAAGCA CTTCTACAGC AAACTGAAGG ACAGACTGAA CTACTGGAAG 
GCCAAAGACC TCTGGCAGAA GATCGACAAG AGAGCCGCTC ATCCCGACTA TGACCAGGGA 
AAAGCATGTC ACCAAAACAA GTGTTTGGTG TTGGGCGCGG GTCCCTGTGG TCTCAGGACA 
GCCATTGAAT TGGCTCTGCT TGGCGCTCAG GTTGTTGTTC TGGAGAAAAG ATCATCTTTC 
ACGAGGAATA ATGTCCTGCA TTTGTGGCCC TTCACCATTA AAGACCTTCT CAACCTCGGG 
GCAAAGAAAT TTTATGGCCG ATTCTGCAGT GGCTCCATCC ATCACATAAG TATCCGTCAG 
CTGCAGTTGA TTCTGCTGAA AGTGGCTCTG TTTTTGGGGG TGGAGGTTCA CACTGGTGTG 
GCTTTTGAAG GTCTAAATGA ACCATCAGGA TCTGCAGGAT GGAGAGCAAA TGTGTCTCCA 
AAGTCCCACC CAGTTGCAGA TTTTCAGTTT GATGTGTTTA TTTCAGCCGG AGGGGGGAAA 
TATGTGCCTG ATGGTTTTAA GATAAAGGAG CTCCGAGGAA AGCTGGCTAT TGGTATCACT 
GCAAACTTTG TAAACCGTCA CACTAAACAA GAGGCTCAGG TGCAGGAGAT CAGCGGTGTG 
GCCCGAATTT ACAACCAGCA GTTTTTCCAG GCTCTTCAGT CTGAGATTGG CGTTGATCTA 
GAAAACATTG TCTACTACAA AGACGACACC CACTATTTTG TGATGACTGC AAAAAAGGCC 
AGCTTGTTAA AGAAAGGAGT CATAAAGCAG GATTTCTCTG ATGCCGATAA GCTCCTGGCT 
CCCTCAAATG TGAATCAGGA GGCACTGCAG GATTACGCAT TCGAAGCATG TGATTTCTCC 
ACAGAGCACT TGCTGCCCAA CCTGGAATTC GCCAAGAACC ACAAAGGCCA AGCGGACGTG 
GCCATGTTTG ACTTTACCTG CATGCAGAGG GCAGAGAGCG CCTCTCTGGT GAAGGAGAGG 
AATGGCAAGA GGCTGCTCAT CGGGCTGGTG GGAGATTCTC TGGTAGAGCC TTTTTGGCCT 
TTGGGCACTG GCATTGCTCG TGGTTTTCTG GGTGCGTTTG ATGCAGCGTG GATGGTCAGG 
AGTTGGGGAA AAGGTGTGCA GCCCATGGAG GTTCTGGCAG AGAGGGAAAG TGTGTATCAG 
TTGCTTTCTC AAACAACTCC AGAAAACACC AGCAAGAACT ACATGGCCTA CAGCATTGAT 
CCAAGCACAC GATACCCGAA CATCAACCTG TCCTCCATCA AACCCAGACA GGTCAAGCGT 
CTGTATGAAG CAGAAGAGCA GGAAAGCAAA CCAAACAAAC TGAAGAAACC TGACATAAAA 
GCTAAACCTA GAAAAGACTC TATGAAGCGT CTGGAGGAGC TGTTGTCCTG GTGTCAGAAG 
AATACAGTGG GATACGAGCA TGTGAAGGTC AAAGATCTGA GCGAGTCTTG GAGGTCTGGT 
CTGGCTCTCT GTGCTCTCAT CCACAGCTTC AGACCTGAGC TTGTTGACAT GTCGGCTCTG 
GATGAGTATA ATATCATCAA GAACAATAAG TTGGCTTTTG ATCTGATGGA GAAAGAGTTC 
GGCATCACCC CCATCATGCG GCCCGGCGAT ATGATGACCT GTGGTAAAAT CGACCAGCTG 
TCCATGGTGG TTTACCTCAC ACAGATACGC AACGCTCTTA CTGAGAAAGA CACACCAGCA 
GCGCAGTCCA ACACACTCTC CCTGTCCAGA AAGCGTTCAG CCGTTGCCTT CCTCAACACA 
CTCAAACGCA ACTCTCTGCA AAGACATAAG GATCGACTGG CATCCGTGAA AGGACCAAGA 
CAGCAAAACA TGAAGGAGAA AGAAGAGAAG AAGGATGTGA AAGAGGAAAG TCTCTCCTCA 
GAGACGAGTG CATGTGAACC CTGTTATTTC TGCAAGAAGC ATCTGTATGT GGTGGAGAGA 
GAAAGTGCAG AGGGAAAGTT CTTCCATCGC AGCTGCTTCA ACTGTTTTCA GTGCGGCTCC 
ACTCTGCGGC AGGGCGGATA CTCTTTTCAC TCTGACAATG GGAGGTTTTA CTGCGAGCTG 
CACTCACTGG CTGAGGAAGA GGAGGGTGAT GAAGGTCATG GAGGCGCACA GAATCATACT 
GAGAACGGAA GTAAGGAGGA CAAAAATGGA GAAACAACAG CGGCTTCATC CCCACCTGCG 
CATCTCTCAA TAAAGCGTAA GGGCTCGTAC AAGATCTCTG TTGACCCTGA CTTTGATGAA 
TCAACTGAGT TTCCTGCTCC AGACCAAGAT GAACCACCTG ATCTTGAGGA GTCCCATCAA 
CCTCCCAAAC CTTCAGAGCT CAGTGCGGAA AACACAAACA TGGAAAACCA ACAACACAAC 
ATCAATCCAG TTCCTGCCCC GCGTGGCTCC AGAGCTCCGC TCCCGAAACC TCGCACTGTC 
CATAATGTGG TGCACGAACC CTGTAATATT CCAGAGGAAG CAGAGCAGAT TCCTGAGGAG 
CCCAAACCCA AACCGTCCCT GCGCAAACTC CAGCAGAGCG AAGAGGAGAA GGTGGATCTG 
TTGAGCCAGG ATTCAGACTC TGAGACACGG GGCTCGTCTT CTGCCGCCTC CACCTCCTCC 
TCATCCAAAC AGCATGAAGA GGAGGGCTAC TGGAGCGGAG GCACGACGTG GGGGAAGAGC 
CATCGTGAGC AACGAAACCG CCCCTGCATA AGGAGGAAGA GTGAACCTCC GCTGCCGCTG 
ACTGGCCATT CTCAGCACGG AAAGATGCGC TCCAAGTTTT CCCCGTGGAA TCTGTCTTCA 
CCGCGGCTCC AGCAGCGATT CAGCGTTCAC AGAGTTCCTG CAGGTCAAAG TCAGCCAGAT 
CAGTATGTAT CAGAAGATGA TAATGAAGAT GATGAAGATG AGGATGAAGA GGACCTTCAG 
GCTGAACATT ATTTGGATTG CGAGGGAGCT GATTTTGAAT TTTCGGATTC AGAGAAACGC 
AACTTAAAGA GGATGAAAAC TCTGGAGCGG AAAGCAAAAA TGACTGAAAT CCAGCGTTTC 
CACAAAGCTC AGTCGATTCA GAGACGTCTG GAAGAGATCG AAGTGACCTT TAAAGAGCTG 
GAGGAGAAAG GAGTGGAGCT GGAGCGAGCT CTGAGAGGAG AGACGGGTAC AGGTGACCCT 
GAAATCATTG ATCAGTGGAT TGAACTGGTC CAGGAGAAAA ACAACCTGCT GTCAGAGGAG 
TCTGATCTCA TGGTGGCGTC TCGACAGCTG GAGCTGGAGG ACAAACAGAG CATGCTGGAG 
ATGGAGCTGC GCCGCTACAT GGAAATGGAT GATTCAGAGA AAAGCCCTGA GCAGCAAAAG 
CACGAGGCAG AGATCCTGCA GGAGATGCTG GACGTGGTAG ACATGAGGGA TTCACTGGTG 
GCCTTTCTGG AGGAGAAGAG GCTGAAGGAG GTCAATGATC AATTTAATTC AAGCCTGGAT 
GCCAAAAGAC GTTCAACCAC AGCCTCCCAG GTGCACTGGG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003201274.1
CDS	161..3805
Translation	MVNPLDSVNPSHALFEGFVQAQTCKETQQNFTELCRHLQVDPKDYKHFYSKLKDRLNYWKAKDLWQKIDKRAAHPDYDQGKACHQNKCLVLGAGPCGLRTAIELALLGAQVVVLEKRSSFTRNNVLHLWPFTIKDLLNLGAKKFYGRFCSGSIHHISIRQLQLILLKVALFLGVEVHTGVAFEGLNEPSGSAGWRANVSPKSHPVADFQFDVFISAGGGKYVPDGFKIKELRGKLAIGITANFVNRHTKQEAQVQEISGVARIYNQQFFQALQSEIGVDLENIVYYKDDTHYFVMTAKKASLLKKGVIKQDFSDADKLLAPSNVNQEALQDYAFEACDFSTEHLLPNLEFAKNHKGQADVAMFDFTCMQRAESASLVKERNGKRLLIGLVGDSLVEPFWPLGTGIARGFLGAFDAAWMVRSWGKGVQPMEVLAERESVYQLLSQTTPENTSKNYMAYSIDPSTRYPNINLSSIKPRQVKRLYEAEEQESKPNKLKKPDIKAKPRKDSMKRLEELLSWCQKNTVGYEHVKVKDLSESWRSGLALCALIHSFRPELVDMSALDEYNIIKNNKLAFDLMEKEFGITPIMRPGDMMTCGKIDQLSMVVYLTQIRNALTEKDTPAAQSNTLSLSRKRSAVAFLNTLKRNSLQRHKDRLASVKGPRQQNMKEKEEKKDVKEESLSSETSACEPCYFCKKHLYVVERESAEGKFFHRSCFNCFQCGSTLRQGGYSFHSDNGRFYCELHSLAEEEEGDEGHGGAQNHTENGSKEDKNGETTAASSPPAHLSIKRKGSYKISVDPDFDESTEFPAPDQDEPPDLEESHQPPKPSELSAENTNMENQQHNINPVPAPRGSRAPLPKPRTVHNVVHEPCNIPEEAEQIPEEPKPKPSLRKLQQSEEEKVDLLSQDSDSETRGSSSAASTSSSSKQHEEEGYWSGGTTWGKSHREQRNRPCIRRKSEPPLPLTGHSQHGKMRSKFSPWNLSSPRLQQRFSVHRVPAGQSQPDQYVSEDDNEDDEDEDEEDLQAEHYLDCEGADFEFSDSEKRNLKRMKTLERKAKMTEIQRFHKAQSIQRRLEEIEVTFKELEEKGVELERALRGETGTGDPEIIDQWIELVQEKNNLLSEESDLMVASRQLELEDKQSMLEMELRRYMEMDDSEKSPEQQKHEAEILQEMLDVVDMRDSLVAFLEEKRLKEVNDQFNSSLDAKRRSTTASQVHWE

Target ORF information:

RefSeq Version	XM_003201226.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 1 (mical1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003201226.5

ORF Insert Sequence:

ATGGTCAACC CGCTGGACTC AGTCAACCCC AGCCATGCTC TGTTTGAGGG CTTTGTCCAG 
GCGCAGACAT GTAAAGAGAC GCAGCAAAAC TTCACCGAGC TCTGCCGCCA CCTGCAGGTC 
GACCCCAAAG ACTACAAGCA CTTCTACAGC AAACTGAAGG ACAGACTGAA CTACTGGAAG 
GCCAAAGACC TCTGGCAGAA GATCGACAAG AGAGCCGCTC ATCCCGACTA TGACCAGGGA 
AAAGCATGTC ACCAAAACAA GTGTTTGGTG TTGGGCGCGG GTCCCTGTGG TCTCAGGACA 
GCCATTGAAT TGGCTCTGCT TGGCGCTCAG GTTGTTGTTC TGGAGAAAAG ATCATCTTTC 
ACGAGGAATA ATGTCCTGCA TTTGTGGCCC TTCACCATTA AAGACCTTCT CAACCTCGGG 
GCAAAGAAAT TTTATGGCCG ATTCTGCAGT GGCTCCATCC ATCACATAAG TATCCGTCAG 
CTGCAGTTGA TTCTGCTGAA AGTGGCTCTG TTTTTGGGGG TGGAGGTTCA CACTGGTGTG 
GCTTTTGAAG GTCTAAATGA ACCATCAGGA TCTGCAGGAT GGAGAGCAAA TGTGTCTCCA 
AAGTCCCACC CAGTTGCAGA TTTTCAGTTT GATGTGTTTA TTTCAGCCGG AGGGGGGAAA 
TATGTGCCTG ATGGTTTTAA GATAAAGGAG CTCCGAGGAA AGCTGGCTAT TGGTATCACT 
GCAAACTTTG TAAACCGTCA CACTAAACAA GAGGCTCAGG TGCAGGAGAT CAGCGGTGTG 
GCCCGAATTT ACAACCAGCA GTTTTTCCAG GCTCTTCAGT CTGAGATTGG CGTTGATCTA 
GAAAACATTG TCTACTACAA AGACGACACC CACTATTTTG TGATGACTGC AAAAAAGGCC 
AGCTTGTTAA AGAAAGGAGT CATAAAGCAG GATTTCTCTG ATGCCGATAA GCTCCTGGCT 
CCCTCAAATG TGAATCAGGA GGCACTGCAG GATTACGCAT TCGAAGCATG TGATTTCTCC 
ACAGAGCACT TGCTGCCCAA CCTGGAATTC GCCAAGAACC ACAAAGGCCA AGCGGACGTG 
GCCATGTTTG ACTTTACCTG CATGCAGAGG GCAGAGAGCG CCTCTCTGGT GAAGGAGAGG 
AATGGCAAGA GGCTGCTCAT CGGGCTGGTG GGAGATTCTC TGGTAGAGCC TTTTTGGCCT 
TTGGGCACTG GCATTGCTCG TGGTTTTCTG GGTGCGTTTG ATGCAGCGTG GATGGTCAGG 
AGTTGGGGAA AAGGTGTGCA GCCCATGGAG GTTCTGGCAG AGAGGGAAAG TGTGTATCAG 
TTGCTTTCTC AAACAACTCC AGAAAACACC AGCAAGAACT ACATGGCCTA CAGCATTGAT 
CCAAGCACAC GATACCCGAA CATCAACCTG TCCTCCATCA AACCCAGACA GGTCAAGCGT 
CTGTATGAAG CAGAAGAGCA GGAAAGCAAA CCAAACAAAC TGAAGAAACC TGACATAAAA 
GCTAAACCTA GAAAAGACTC TATGAAGCGT CTGGAGGAGC TGTTGTCCTG GTGTCAGAAG 
AATACAGTGG GATACGAGCA TGTGAAGGTC AAAGATCTGA GCGAGTCTTG GAGGTCTGGT 
CTGGCTCTCT GTGCTCTCAT CCACAGCTTC AGACCTGAGC TTGTTGACAT GTCGGCTCTG 
GATGAGTATA ATATCATCAA GAACAATAAG TTGGCTTTTG ATCTGATGGA GAAAGAGTTC 
GGCATCACCC CCATCATGCG GCCCGGCGAT ATGATGACCT GTGGTAAAAT CGACCAGCTG 
TCCATGGTGG TTTACCTCAC ACAGATACGC AACGCTCTTA CTGAGAAAGA CACACCAGCA 
GCGCAGTCCA ACACACTCTC CCTGTCCAGA AAGCGTTCAG CCGTTGCCTT CCTCAACACA 
CTCAAACGCA ACTCTCTGCA AAGACATAAG GATCGACTGG CATCCGTGAA AGGACCAAGA 
CAGCAAAACA TGAAGGAGAA AGAAGAGAAG AAGGATGTGA AAGAGGAAAG TCTCTCCTCA 
GAGACGAGTG CATGTGAACC CTGTTATTTC TGCAAGAAGC ATCTGTATGT GGTGGAGAGA 
GAAAGTGCAG AGGGAAAGTT CTTCCATCGC AGCTGCTTCA ACTGTTTTCA GTGCGGCTCC 
ACTCTGCGGC AGGGCGGATA CTCTTTTCAC TCTGACAATG GGAGGTTTTA CTGCGAGCTG 
CACTCACTGG CTGAGGAAGA GGAGGGTGAT GAAGGTCATG GAGGCGCACA GAATCATACT 
GAGAACGGAA GTAAGGAGGA CAAAAATGGA GAAACAACAG CGGCTTCATC CCCACCTGCG 
CATCTCTCAA TAAAGCGTAA GGGCTCGTAC AAGATCTCTG TTGACCCTGA CTTTGATGAA 
TCAACTGAGT TTCCTGCTCC AGACCAAGAT GAACCACCTG ATCTTGAGGA GTCCCATCAA 
CCTCCCAAAC CTTCAGAGCT CAGTGCGGAA AACACAAACA TGGAAAACCA ACAACACAAC 
ATCAATCCAG TTCCTGCCCC GCGTGGCTCC AGAGCTCCGC TCCCGAAACC TCGCACTGTC 
CATAATGTGG TGCACGAACC CTGTAATATT CCAGAGGAAG CAGAGCAGAT TCCTGAGGAG 
CCCAAACCCA AACCGTCCCT GCGCAAACTC CAGCAGAGCG AAGAGGAGAA GGTGGATCTG 
TTGAGCCAGG ATTCAGACTC TGAGACACGG GGCTCGTCTT CTGCCGCCTC CACCTCCTCC 
TCATCCAAAC AGCATGAAGA GGAGGGCTAC TGGAGCGGAG GCACGACGTG GGGGAAGAGC 
CATCGTGAGC AACGAAACCG CCCCTGCATA AGGAGGAAGA GTGAACCTCC GCTGCCGCTG 
ACTGGCCATT CTCAGCACGG AAAGATGCGC TCCAAGTTTT CCCCGTGGAA TCTGTCTTCA 
CCGCGGCTCC AGCAGCGATT CAGCGTTCAC AGAGTTCCTG CAGGTCAAAG TCAGCCAGAT 
CAGTATGTAT CAGAAGATGA TAATGAAGAT GATGAAGATG AGGATGAAGA GGACCTTCAG 
GCTGAACATT ATTTGGATTG CGAGGGAGCT GATTTTGAAT TTTCGGATTC AGAGAAACGC 
AACTTAAAGA GGATGAAAAC TCTGGAGCGG AAAGCAAAAA TGACTGAAAT CCAGCGTTTC 
CACAAAGCTC AGTCGATTCA GAGACGTCTG GAAGAGATCG AAGTGACCTT TAAAGAGCTG 
GAGGAGAAAG GAGTGGAGCT GGAGCGAGCT CTGAGAGGAG AGACGGGTAC AGGTGACCCT 
GAAATCATTG ATCAGTGGAT TGAACTGGTC CAGGAGAAAA ACAACCTGCT GTCAGAGGAG 
TCTGATCTCA TGGTGGCGTC TCGACAGCTG GAGCTGGAGG ACAAACAGAG CATGCTGGAG 
ATGGAGCTGC GCCGCTACAT GGAAATGGAT GATTCAGAGA AAAGCCCTGA GCAGCAAAAG 
CACGAGGCAG AGATCCTGCA GGAGATGCTG GACGTGGTAG ACATGAGGGA TTCACTGGTG 
GCCTTTCTGG AGGAGAAGAG GCTGAAGGAG GTCAATGATC AATTTAATTC AAGCCTGGAT 
GCCAAAAGAC GTTCAACCAC AGCCTCCCAG GTGCACTGGG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL1 ( XM_009297077.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009295352.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 1 (mical1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa28049
Clone ID Related Accession (Same CDS sequence)	XM_003201226.5 , NM_001316730.1 , XM_009297077.2 , XM_003201226.4
Accession Version	XM_009297077.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein-methionine sulfoxide oxidase mical1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297077.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAACC CGCTGGACTC AGTCAACCCC AGCCATGCTC TGTTTGAGGG CTTTGTCCAG 
GCGCAGACAT GTAAAGAGAC GCAGCAAAAC TTCACCGAGC TCTGCCGCCA CCTGCAGGTC 
GACCCCAAAG ACTACAAGCA CTTCTACAGC AAACTGAAGG ACAGACTGAA CTACTGGAAG 
GCCAAAGACC TCTGGCAGAA GATCGACAAG AGAGCCGCTC ATCCCGACTA TGACCAGGGA 
AAAGCATGTC ACCAAAACAA GTGTTTGGTG TTGGGCGCGG GTCCCTGTGG TCTCAGGACA 
GCCATTGAAT TGGCTCTGCT TGGCGCTCAG GTTGTTGTTC TGGAGAAAAG ATCATCTTTC 
ACGAGGAATA ATGTCCTGCA TTTGTGGCCC TTCACCATTA AAGACCTTCT CAACCTCGGG 
GCAAAGAAAT TTTATGGCCG ATTCTGCAGT GGCTCCATCC ATCACATAAG TATCCGTCAG 
CTGCAGTTGA TTCTGCTGAA AGTGGCTCTG TTTTTGGGGG TGGAGGTTCA CACTGGTGTG 
GCTTTTGAAG GTCTAAATGA ACCATCAGGA TCTGCAGGAT GGAGAGCAAA TGTGTCTCCA 
AAGTCCCACC CAGTTGCAGA TTTTCAGTTT GATGTGTTTA TTTCAGCCGG AGGGGGGAAA 
TATGTGCCTG ATGGTTTTAA GATAAAGGAG CTCCGAGGAA AGCTGGCTAT TGGTATCACT 
GCAAACTTTG TAAACCGTCA CACTAAACAA GAGGCTCAGG TGCAGGAGAT CAGCGGTGTG 
GCCCGAATTT ACAACCAGCA GTTTTTCCAG GCTCTTCAGT CTGAGATTGG CGTTGATCTA 
GAAAACATTG TCTACTACAA AGACGACACC CACTATTTTG TGATGACTGC AAAAAAGGCC 
AGCTTGTTAA AGAAAGGAGT CATAAAGCAG GATTTCTCTG ATGCCGATAA GCTCCTGGCT 
CCCTCAAATG TGAATCAGGA GGCACTGCAG GATTACGCAT TCGAAGCATG TGATTTCTCC 
ACAGAGCACT TGCTGCCCAA CCTGGAATTC GCCAAGAACC ACAAAGGCCA AGCGGACGTG 
GCCATGTTTG ACTTTACCTG CATGCAGAGG GCAGAGAGCG CCTCTCTGGT GAAGGAGAGG 
AATGGCAAGA GGCTGCTCAT CGGGCTGGTG GGAGATTCTC TGGTAGAGCC TTTTTGGCCT 
TTGGGCACTG GCATTGCTCG TGGTTTTCTG GGTGCGTTTG ATGCAGCGTG GATGGTCAGG 
AGTTGGGGAA AAGGTGTGCA GCCCATGGAG GTTCTGGCAG AGAGGGAAAG TGTGTATCAG 
TTGCTTTCTC AAACAACTCC AGAAAACACC AGCAAGAACT ACATGGCCTA CAGCATTGAT 
CCAAGCACAC GATACCCGAA CATCAACCTG TCCTCCATCA AACCCAGACA GGTCAAGCGT 
CTGTATGAAG CAGAAGAGCA GGAAAGCAAA CCAAACAAAC TGAAGAAACC TGACATAAAA 
GCTAAACCTA GAAAAGACTC TATGAAGCGT CTGGAGGAGC TGTTGTCCTG GTGTCAGAAG 
AATACAGTGG GATACGAGCA TGTGAAGGTC AAAGATCTGA GCGAGTCTTG GAGGTCTGGT 
CTGGCTCTCT GTGCTCTCAT CCACAGCTTC AGACCTGAGC TTGTTGACAT GTCGGCTCTG 
GATGAGTATA ATATCATCAA GAACAATAAG TTGGCTTTTG ATCTGATGGA GAAAGAGTTC 
GGCATCACCC CCATCATGCG GCCCGGCGAT ATGATGACCT GTGGTAAAAT CGACCAGCTG 
TCCATGGTGG TTTACCTCAC ACAGATACGC AACGCTCTTA CTGAGAAAGA CACACCAGCA 
GCGCAGTCCA ACACACTCTC CCTGTCCAGA AAGCGTTCAG CCGTTGCCTT CCTCAACACA 
CTCAAACGCA ACTCTCTGCA AAGACATAAG GATCGACTGG CATCCGTGAA AGGACCAAGA 
CAGCAAAACA TGAAGGAGAA AGAAGAGAAG AAGGATGTGA AAGAGGAAAG TCTCTCCTCA 
GAGACGAGTG CATGTGAACC CTGTTATTTC TGCAAGAAGC ATCTGTATGT GGTGGAGAGA 
GAAAGTGCAG AGGGAAAGTT CTTCCATCGC AGCTGCTTCA ACTGTTTTCA GTGCGGCTCC 
ACTCTGCGGC AGGGCGGATA CTCTTTTCAC TCTGACAATG GGAGGTTTTA CTGCGAGCTG 
CACTCACTGG CTGAGGAAGA GGAGGGTGAT GAAGGTCATG GAGGCGCACA GAATCATACT 
GAGAACGGAA GTAAGGAGGA CAAAAATGGA GAAACAACAG CGGCTTCATC CCCACCTGCG 
CATCTCTCAA TAAAGCGTAA GGGCTCGTAC AAGATCTCTG TTGACCCTGA CTTTGATGAA 
TCAACTGAGT TTCCTGCTCC AGACCAAGAT GAACCACCTG ATCTTGAGGA GTCCCATCAA 
CCTCCCAAAC CTTCAGAGCT CAGTGCGGAA AACACAAACA TGGAAAACCA ACAACACAAC 
ATCAATCCAG TTCCTGCCCC GCGTGGCTCC AGAGCTCCGC TCCCGAAACC TCGCACTGTC 
CATAATGTGG TGCACGAACC CTGTAATATT CCAGAGGAAG CAGAGCAGAT TCCTGAGGAG 
CCCAAACCCA AACCGTCCCT GCGCAAACTC CAGCAGAGCG AAGAGGAGAA GGTGGATCTG 
TTGAGCCAGG ATTCAGACTC TGAGACACGG GGCTCGTCTT CTGCCGCCTC CACCTCCTCC 
TCATCCAAAC AGCATGAAGA GGAGGGCTAC TGGAGCGGAG GCACGACGTG GGGGAAGAGC 
CATCGTGAGC AACGAAACCG CCCCTGCATA AGGAGGAAGA GTGAACCTCC GCTGCCGCTG 
ACTGGCCATT CTCAGCACGG AAAGATGCGC TCCAAGTTTT CCCCGTGGAA TCTGTCTTCA 
CCGCGGCTCC AGCAGCGATT CAGCGTTCAC AGAGTTCCTG CAGGTCAAAG TCAGCCAGAT 
CAGTATGTAT CAGAAGATGA TAATGAAGAT GATGAAGATG AGGATGAAGA GGACCTTCAG 
GCTGAACATT ATTTGGATTG CGAGGGAGCT GATTTTGAAT TTTCGGATTC AGAGAAACGC 
AACTTAAAGA GGATGAAAAC TCTGGAGCGG AAAGCAAAAA TGACTGAAAT CCAGCGTTTC 
CACAAAGCTC AGTCGATTCA GAGACGTCTG GAAGAGATCG AAGTGACCTT TAAAGAGCTG 
GAGGAGAAAG GAGTGGAGCT GGAGCGAGCT CTGAGAGGAG AGACGGGTAC AGGTGACCCT 
GAAATCATTG ATCAGTGGAT TGAACTGGTC CAGGAGAAAA ACAACCTGCT GTCAGAGGAG 
TCTGATCTCA TGGTGGCGTC TCGACAGCTG GAGCTGGAGG ACAAACAGAG CATGCTGGAG 
ATGGAGCTGC GCCGCTACAT GGAAATGGAT GATTCAGAGA AAAGCCCTGA GCAGCAAAAG 
CACGAGGCAG AGATCCTGCA GGAGATGCTG GACGTGGTAG ACATGAGGGA TTCACTGGTG 
GCCTTTCTGG AGGAGAAGAG GCTGAAGGAG GTCAATGATC AATTTAATTC AAGCCTGGAT 
GCCAAAAGAC GTTCAACCAC AGCCTCCCAG GTGCACTGGG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295352.1
CDS	63..3707
Translation	MVNPLDSVNPSHALFEGFVQAQTCKETQQNFTELCRHLQVDPKDYKHFYSKLKDRLNYWKAKDLWQKIDKRAAHPDYDQGKACHQNKCLVLGAGPCGLRTAIELALLGAQVVVLEKRSSFTRNNVLHLWPFTIKDLLNLGAKKFYGRFCSGSIHHISIRQLQLILLKVALFLGVEVHTGVAFEGLNEPSGSAGWRANVSPKSHPVADFQFDVFISAGGGKYVPDGFKIKELRGKLAIGITANFVNRHTKQEAQVQEISGVARIYNQQFFQALQSEIGVDLENIVYYKDDTHYFVMTAKKASLLKKGVIKQDFSDADKLLAPSNVNQEALQDYAFEACDFSTEHLLPNLEFAKNHKGQADVAMFDFTCMQRAESASLVKERNGKRLLIGLVGDSLVEPFWPLGTGIARGFLGAFDAAWMVRSWGKGVQPMEVLAERESVYQLLSQTTPENTSKNYMAYSIDPSTRYPNINLSSIKPRQVKRLYEAEEQESKPNKLKKPDIKAKPRKDSMKRLEELLSWCQKNTVGYEHVKVKDLSESWRSGLALCALIHSFRPELVDMSALDEYNIIKNNKLAFDLMEKEFGITPIMRPGDMMTCGKIDQLSMVVYLTQIRNALTEKDTPAAQSNTLSLSRKRSAVAFLNTLKRNSLQRHKDRLASVKGPRQQNMKEKEEKKDVKEESLSSETSACEPCYFCKKHLYVVERESAEGKFFHRSCFNCFQCGSTLRQGGYSFHSDNGRFYCELHSLAEEEEGDEGHGGAQNHTENGSKEDKNGETTAASSPPAHLSIKRKGSYKISVDPDFDESTEFPAPDQDEPPDLEESHQPPKPSELSAENTNMENQQHNINPVPAPRGSRAPLPKPRTVHNVVHEPCNIPEEAEQIPEEPKPKPSLRKLQQSEEEKVDLLSQDSDSETRGSSSAASTSSSSKQHEEEGYWSGGTTWGKSHREQRNRPCIRRKSEPPLPLTGHSQHGKMRSKFSPWNLSSPRLQQRFSVHRVPAGQSQPDQYVSEDDNEDDEDEDEEDLQAEHYLDCEGADFEFSDSEKRNLKRMKTLERKAKMTEIQRFHKAQSIQRRLEEIEVTFKELEEKGVELERALRGETGTGDPEIIDQWIELVQEKNNLLSEESDLMVASRQLELEDKQSMLEMELRRYMEMDDSEKSPEQQKHEAEILQEMLDVVDMRDSLVAFLEEKRLKEVNDQFNSSLDAKRRSTTASQVHWE

Target ORF information:

RefSeq Version	XM_009297077.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 1 (mical1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297077.2

ORF Insert Sequence:

ATGGTCAACC CGCTGGACTC AGTCAACCCC AGCCATGCTC TGTTTGAGGG CTTTGTCCAG 
GCGCAGACAT GTAAAGAGAC GCAGCAAAAC TTCACCGAGC TCTGCCGCCA CCTGCAGGTC 
GACCCCAAAG ACTACAAGCA CTTCTACAGC AAACTGAAGG ACAGACTGAA CTACTGGAAG 
GCCAAAGACC TCTGGCAGAA GATCGACAAG AGAGCCGCTC ATCCCGACTA TGACCAGGGA 
AAAGCATGTC ACCAAAACAA GTGTTTGGTG TTGGGCGCGG GTCCCTGTGG TCTCAGGACA 
GCCATTGAAT TGGCTCTGCT TGGCGCTCAG GTTGTTGTTC TGGAGAAAAG ATCATCTTTC 
ACGAGGAATA ATGTCCTGCA TTTGTGGCCC TTCACCATTA AAGACCTTCT CAACCTCGGG 
GCAAAGAAAT TTTATGGCCG ATTCTGCAGT GGCTCCATCC ATCACATAAG TATCCGTCAG 
CTGCAGTTGA TTCTGCTGAA AGTGGCTCTG TTTTTGGGGG TGGAGGTTCA CACTGGTGTG 
GCTTTTGAAG GTCTAAATGA ACCATCAGGA TCTGCAGGAT GGAGAGCAAA TGTGTCTCCA 
AAGTCCCACC CAGTTGCAGA TTTTCAGTTT GATGTGTTTA TTTCAGCCGG AGGGGGGAAA 
TATGTGCCTG ATGGTTTTAA GATAAAGGAG CTCCGAGGAA AGCTGGCTAT TGGTATCACT 
GCAAACTTTG TAAACCGTCA CACTAAACAA GAGGCTCAGG TGCAGGAGAT CAGCGGTGTG 
GCCCGAATTT ACAACCAGCA GTTTTTCCAG GCTCTTCAGT CTGAGATTGG CGTTGATCTA 
GAAAACATTG TCTACTACAA AGACGACACC CACTATTTTG TGATGACTGC AAAAAAGGCC 
AGCTTGTTAA AGAAAGGAGT CATAAAGCAG GATTTCTCTG ATGCCGATAA GCTCCTGGCT 
CCCTCAAATG TGAATCAGGA GGCACTGCAG GATTACGCAT TCGAAGCATG TGATTTCTCC 
ACAGAGCACT TGCTGCCCAA CCTGGAATTC GCCAAGAACC ACAAAGGCCA AGCGGACGTG 
GCCATGTTTG ACTTTACCTG CATGCAGAGG GCAGAGAGCG CCTCTCTGGT GAAGGAGAGG 
AATGGCAAGA GGCTGCTCAT CGGGCTGGTG GGAGATTCTC TGGTAGAGCC TTTTTGGCCT 
TTGGGCACTG GCATTGCTCG TGGTTTTCTG GGTGCGTTTG ATGCAGCGTG GATGGTCAGG 
AGTTGGGGAA AAGGTGTGCA GCCCATGGAG GTTCTGGCAG AGAGGGAAAG TGTGTATCAG 
TTGCTTTCTC AAACAACTCC AGAAAACACC AGCAAGAACT ACATGGCCTA CAGCATTGAT 
CCAAGCACAC GATACCCGAA CATCAACCTG TCCTCCATCA AACCCAGACA GGTCAAGCGT 
CTGTATGAAG CAGAAGAGCA GGAAAGCAAA CCAAACAAAC TGAAGAAACC TGACATAAAA 
GCTAAACCTA GAAAAGACTC TATGAAGCGT CTGGAGGAGC TGTTGTCCTG GTGTCAGAAG 
AATACAGTGG GATACGAGCA TGTGAAGGTC AAAGATCTGA GCGAGTCTTG GAGGTCTGGT 
CTGGCTCTCT GTGCTCTCAT CCACAGCTTC AGACCTGAGC TTGTTGACAT GTCGGCTCTG 
GATGAGTATA ATATCATCAA GAACAATAAG TTGGCTTTTG ATCTGATGGA GAAAGAGTTC 
GGCATCACCC CCATCATGCG GCCCGGCGAT ATGATGACCT GTGGTAAAAT CGACCAGCTG 
TCCATGGTGG TTTACCTCAC ACAGATACGC AACGCTCTTA CTGAGAAAGA CACACCAGCA 
GCGCAGTCCA ACACACTCTC CCTGTCCAGA AAGCGTTCAG CCGTTGCCTT CCTCAACACA 
CTCAAACGCA ACTCTCTGCA AAGACATAAG GATCGACTGG CATCCGTGAA AGGACCAAGA 
CAGCAAAACA TGAAGGAGAA AGAAGAGAAG AAGGATGTGA AAGAGGAAAG TCTCTCCTCA 
GAGACGAGTG CATGTGAACC CTGTTATTTC TGCAAGAAGC ATCTGTATGT GGTGGAGAGA 
GAAAGTGCAG AGGGAAAGTT CTTCCATCGC AGCTGCTTCA ACTGTTTTCA GTGCGGCTCC 
ACTCTGCGGC AGGGCGGATA CTCTTTTCAC TCTGACAATG GGAGGTTTTA CTGCGAGCTG 
CACTCACTGG CTGAGGAAGA GGAGGGTGAT GAAGGTCATG GAGGCGCACA GAATCATACT 
GAGAACGGAA GTAAGGAGGA CAAAAATGGA GAAACAACAG CGGCTTCATC CCCACCTGCG 
CATCTCTCAA TAAAGCGTAA GGGCTCGTAC AAGATCTCTG TTGACCCTGA CTTTGATGAA 
TCAACTGAGT TTCCTGCTCC AGACCAAGAT GAACCACCTG ATCTTGAGGA GTCCCATCAA 
CCTCCCAAAC CTTCAGAGCT CAGTGCGGAA AACACAAACA TGGAAAACCA ACAACACAAC 
ATCAATCCAG TTCCTGCCCC GCGTGGCTCC AGAGCTCCGC TCCCGAAACC TCGCACTGTC 
CATAATGTGG TGCACGAACC CTGTAATATT CCAGAGGAAG CAGAGCAGAT TCCTGAGGAG 
CCCAAACCCA AACCGTCCCT GCGCAAACTC CAGCAGAGCG AAGAGGAGAA GGTGGATCTG 
TTGAGCCAGG ATTCAGACTC TGAGACACGG GGCTCGTCTT CTGCCGCCTC CACCTCCTCC 
TCATCCAAAC AGCATGAAGA GGAGGGCTAC TGGAGCGGAG GCACGACGTG GGGGAAGAGC 
CATCGTGAGC AACGAAACCG CCCCTGCATA AGGAGGAAGA GTGAACCTCC GCTGCCGCTG 
ACTGGCCATT CTCAGCACGG AAAGATGCGC TCCAAGTTTT CCCCGTGGAA TCTGTCTTCA 
CCGCGGCTCC AGCAGCGATT CAGCGTTCAC AGAGTTCCTG CAGGTCAAAG TCAGCCAGAT 
CAGTATGTAT CAGAAGATGA TAATGAAGAT GATGAAGATG AGGATGAAGA GGACCTTCAG 
GCTGAACATT ATTTGGATTG CGAGGGAGCT GATTTTGAAT TTTCGGATTC AGAGAAACGC 
AACTTAAAGA GGATGAAAAC TCTGGAGCGG AAAGCAAAAA TGACTGAAAT CCAGCGTTTC 
CACAAAGCTC AGTCGATTCA GAGACGTCTG GAAGAGATCG AAGTGACCTT TAAAGAGCTG 
GAGGAGAAAG GAGTGGAGCT GGAGCGAGCT CTGAGAGGAG AGACGGGTAC AGGTGACCCT 
GAAATCATTG ATCAGTGGAT TGAACTGGTC CAGGAGAAAA ACAACCTGCT GTCAGAGGAG 
TCTGATCTCA TGGTGGCGTC TCGACAGCTG GAGCTGGAGG ACAAACAGAG CATGCTGGAG 
ATGGAGCTGC GCCGCTACAT GGAAATGGAT GATTCAGAGA AAAGCCCTGA GCAGCAAAAG 
CACGAGGCAG AGATCCTGCA GGAGATGCTG GACGTGGTAG ACATGAGGGA TTCACTGGTG 
GCCTTTCTGG AGGAGAAGAG GCTGAAGGAG GTCAATGATC AATTTAATTC AAGCCTGGAT 
GCCAAAAGAC GTTCAACCAC AGCCTCCCAG GTGCACTGGG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL1 ( XM_003201226.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_003201274.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 1 (mical1), transcript variant X1, mRNA.

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CloneID	ODa28049
Clone ID Related Accession (Same CDS sequence)	XM_003201226.5 , NM_001316730.1 , XM_009297077.2 , XM_003201226.4
Accession Version	XM_003201226.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein-methionine sulfoxide oxidase mical1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_003201226.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAACC CGCTGGACTC AGTCAACCCC AGCCATGCTC TGTTTGAGGG CTTTGTCCAG 
GCGCAGACAT GTAAAGAGAC GCAGCAAAAC TTCACCGAGC TCTGCCGCCA CCTGCAGGTC 
GACCCCAAAG ACTACAAGCA CTTCTACAGC AAACTGAAGG ACAGACTGAA CTACTGGAAG 
GCCAAAGACC TCTGGCAGAA GATCGACAAG AGAGCCGCTC ATCCCGACTA TGACCAGGGA 
AAAGCATGTC ACCAAAACAA GTGTTTGGTG TTGGGCGCGG GTCCCTGTGG TCTCAGGACA 
GCCATTGAAT TGGCTCTGCT TGGCGCTCAG GTTGTTGTTC TGGAGAAAAG ATCATCTTTC 
ACGAGGAATA ATGTCCTGCA TTTGTGGCCC TTCACCATTA AAGACCTTCT CAACCTCGGG 
GCAAAGAAAT TTTATGGCCG ATTCTGCAGT GGCTCCATCC ATCACATAAG TATCCGTCAG 
CTGCAGTTGA TTCTGCTGAA AGTGGCTCTG TTTTTGGGGG TGGAGGTTCA CACTGGTGTG 
GCTTTTGAAG GTCTAAATGA ACCATCAGGA TCTGCAGGAT GGAGAGCAAA TGTGTCTCCA 
AAGTCCCACC CAGTTGCAGA TTTTCAGTTT GATGTGTTTA TTTCAGCCGG AGGGGGGAAA 
TATGTGCCTG ATGGTTTTAA GATAAAGGAG CTCCGAGGAA AGCTGGCTAT TGGTATCACT 
GCAAACTTTG TAAACCGTCA CACTAAACAA GAGGCTCAGG TGCAGGAGAT CAGCGGTGTG 
GCCCGAATTT ACAACCAGCA GTTTTTCCAG GCTCTTCAGT CTGAGATTGG CGTTGATCTA 
GAAAACATTG TCTACTACAA AGACGACACC CACTATTTTG TGATGACTGC AAAAAAGGCC 
AGCTTGTTAA AGAAAGGAGT CATAAAGCAG GATTTCTCTG ATGCCGATAA GCTCCTGGCT 
CCCTCAAATG TGAATCAGGA GGCACTGCAG GATTACGCAT TCGAAGCATG TGATTTCTCC 
ACAGAGCACT TGCTGCCCAA CCTGGAATTC GCCAAGAACC ACAAAGGCCA AGCGGACGTG 
GCCATGTTTG ACTTTACCTG CATGCAGAGG GCAGAGAGCG CCTCTCTGGT GAAGGAGAGG 
AATGGCAAGA GGCTGCTCAT CGGGCTGGTG GGAGATTCTC TGGTAGAGCC TTTTTGGCCT 
TTGGGCACTG GCATTGCTCG TGGTTTTCTG GGTGCGTTTG ATGCAGCGTG GATGGTCAGG 
AGTTGGGGAA AAGGTGTGCA GCCCATGGAG GTTCTGGCAG AGAGGGAAAG TGTGTATCAG 
TTGCTTTCTC AAACAACTCC AGAAAACACC AGCAAGAACT ACATGGCCTA CAGCATTGAT 
CCAAGCACAC GATACCCGAA CATCAACCTG TCCTCCATCA AACCCAGACA GGTCAAGCGT 
CTGTATGAAG CAGAAGAGCA GGAAAGCAAA CCAAACAAAC TGAAGAAACC TGACATAAAA 
GCTAAACCTA GAAAAGACTC TATGAAGCGT CTGGAGGAGC TGTTGTCCTG GTGTCAGAAG 
AATACAGTGG GATACGAGCA TGTGAAGGTC AAAGATCTGA GCGAGTCTTG GAGGTCTGGT 
CTGGCTCTCT GTGCTCTCAT CCACAGCTTC AGACCTGAGC TTGTTGACAT GTCGGCTCTG 
GATGAGTATA ATATCATCAA GAACAATAAG TTGGCTTTTG ATCTGATGGA GAAAGAGTTC 
GGCATCACCC CCATCATGCG GCCCGGCGAT ATGATGACCT GTGGTAAAAT CGACCAGCTG 
TCCATGGTGG TTTACCTCAC ACAGATACGC AACGCTCTTA CTGAGAAAGA CACACCAGCA 
GCGCAGTCCA ACACACTCTC CCTGTCCAGA AAGCGTTCAG CCGTTGCCTT CCTCAACACA 
CTCAAACGCA ACTCTCTGCA AAGACATAAG GATCGACTGG CATCCGTGAA AGGACCAAGA 
CAGCAAAACA TGAAGGAGAA AGAAGAGAAG AAGGATGTGA AAGAGGAAAG TCTCTCCTCA 
GAGACGAGTG CATGTGAACC CTGTTATTTC TGCAAGAAGC ATCTGTATGT GGTGGAGAGA 
GAAAGTGCAG AGGGAAAGTT CTTCCATCGC AGCTGCTTCA ACTGTTTTCA GTGCGGCTCC 
ACTCTGCGGC AGGGCGGATA CTCTTTTCAC TCTGACAATG GGAGGTTTTA CTGCGAGCTG 
CACTCACTGG CTGAGGAAGA GGAGGGTGAT GAAGGTCATG GAGGCGCACA GAATCATACT 
GAGAACGGAA GTAAGGAGGA CAAAAATGGA GAAACAACAG CGGCTTCATC CCCACCTGCG 
CATCTCTCAA TAAAGCGTAA GGGCTCGTAC AAGATCTCTG TTGACCCTGA CTTTGATGAA 
TCAACTGAGT TTCCTGCTCC AGACCAAGAT GAACCACCTG ATCTTGAGGA GTCCCATCAA 
CCTCCCAAAC CTTCAGAGCT CAGTGCGGAA AACACAAACA TGGAAAACCA ACAACACAAC 
ATCAATCCAG TTCCTGCCCC GCGTGGCTCC AGAGCTCCGC TCCCGAAACC TCGCACTGTC 
CATAATGTGG TGCACGAACC CTGTAATATT CCAGAGGAAG CAGAGCAGAT TCCTGAGGAG 
CCCAAACCCA AACCGTCCCT GCGCAAACTC CAGCAGAGCG AAGAGGAGAA GGTGGATCTG 
TTGAGCCAGG ATTCAGACTC TGAGACACGG GGCTCGTCTT CTGCCGCCTC CACCTCCTCC 
TCATCCAAAC AGCATGAAGA GGAGGGCTAC TGGAGCGGAG GCACGACGTG GGGGAAGAGC 
CATCGTGAGC AACGAAACCG CCCCTGCATA AGGAGGAAGA GTGAACCTCC GCTGCCGCTG 
ACTGGCCATT CTCAGCACGG AAAGATGCGC TCCAAGTTTT CCCCGTGGAA TCTGTCTTCA 
CCGCGGCTCC AGCAGCGATT CAGCGTTCAC AGAGTTCCTG CAGGTCAAAG TCAGCCAGAT 
CAGTATGTAT CAGAAGATGA TAATGAAGAT GATGAAGATG AGGATGAAGA GGACCTTCAG 
GCTGAACATT ATTTGGATTG CGAGGGAGCT GATTTTGAAT TTTCGGATTC AGAGAAACGC 
AACTTAAAGA GGATGAAAAC TCTGGAGCGG AAAGCAAAAA TGACTGAAAT CCAGCGTTTC 
CACAAAGCTC AGTCGATTCA GAGACGTCTG GAAGAGATCG AAGTGACCTT TAAAGAGCTG 
GAGGAGAAAG GAGTGGAGCT GGAGCGAGCT CTGAGAGGAG AGACGGGTAC AGGTGACCCT 
GAAATCATTG ATCAGTGGAT TGAACTGGTC CAGGAGAAAA ACAACCTGCT GTCAGAGGAG 
TCTGATCTCA TGGTGGCGTC TCGACAGCTG GAGCTGGAGG ACAAACAGAG CATGCTGGAG 
ATGGAGCTGC GCCGCTACAT GGAAATGGAT GATTCAGAGA AAAGCCCTGA GCAGCAAAAG 
CACGAGGCAG AGATCCTGCA GGAGATGCTG GACGTGGTAG ACATGAGGGA TTCACTGGTG 
GCCTTTCTGG AGGAGAAGAG GCTGAAGGAG GTCAATGATC AATTTAATTC AAGCCTGGAT 
GCCAAAAGAC GTTCAACCAC AGCCTCCCAG GTGCACTGGG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003201274.1
CDS	125..3769
Translation	MVNPLDSVNPSHALFEGFVQAQTCKETQQNFTELCRHLQVDPKDYKHFYSKLKDRLNYWKAKDLWQKIDKRAAHPDYDQGKACHQNKCLVLGAGPCGLRTAIELALLGAQVVVLEKRSSFTRNNVLHLWPFTIKDLLNLGAKKFYGRFCSGSIHHISIRQLQLILLKVALFLGVEVHTGVAFEGLNEPSGSAGWRANVSPKSHPVADFQFDVFISAGGGKYVPDGFKIKELRGKLAIGITANFVNRHTKQEAQVQEISGVARIYNQQFFQALQSEIGVDLENIVYYKDDTHYFVMTAKKASLLKKGVIKQDFSDADKLLAPSNVNQEALQDYAFEACDFSTEHLLPNLEFAKNHKGQADVAMFDFTCMQRAESASLVKERNGKRLLIGLVGDSLVEPFWPLGTGIARGFLGAFDAAWMVRSWGKGVQPMEVLAERESVYQLLSQTTPENTSKNYMAYSIDPSTRYPNINLSSIKPRQVKRLYEAEEQESKPNKLKKPDIKAKPRKDSMKRLEELLSWCQKNTVGYEHVKVKDLSESWRSGLALCALIHSFRPELVDMSALDEYNIIKNNKLAFDLMEKEFGITPIMRPGDMMTCGKIDQLSMVVYLTQIRNALTEKDTPAAQSNTLSLSRKRSAVAFLNTLKRNSLQRHKDRLASVKGPRQQNMKEKEEKKDVKEESLSSETSACEPCYFCKKHLYVVERESAEGKFFHRSCFNCFQCGSTLRQGGYSFHSDNGRFYCELHSLAEEEEGDEGHGGAQNHTENGSKEDKNGETTAASSPPAHLSIKRKGSYKISVDPDFDESTEFPAPDQDEPPDLEESHQPPKPSELSAENTNMENQQHNINPVPAPRGSRAPLPKPRTVHNVVHEPCNIPEEAEQIPEEPKPKPSLRKLQQSEEEKVDLLSQDSDSETRGSSSAASTSSSSKQHEEEGYWSGGTTWGKSHREQRNRPCIRRKSEPPLPLTGHSQHGKMRSKFSPWNLSSPRLQQRFSVHRVPAGQSQPDQYVSEDDNEDDEDEDEEDLQAEHYLDCEGADFEFSDSEKRNLKRMKTLERKAKMTEIQRFHKAQSIQRRLEEIEVTFKELEEKGVELERALRGETGTGDPEIIDQWIELVQEKNNLLSEESDLMVASRQLELEDKQSMLEMELRRYMEMDDSEKSPEQQKHEAEILQEMLDVVDMRDSLVAFLEEKRLKEVNDQFNSSLDAKRRSTTASQVHWE

Target ORF information:

RefSeq Version	XM_003201226.4
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 1 (mical1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003201226.4

ORF Insert Sequence:

ATGGTCAACC CGCTGGACTC AGTCAACCCC AGCCATGCTC TGTTTGAGGG CTTTGTCCAG 
GCGCAGACAT GTAAAGAGAC GCAGCAAAAC TTCACCGAGC TCTGCCGCCA CCTGCAGGTC 
GACCCCAAAG ACTACAAGCA CTTCTACAGC AAACTGAAGG ACAGACTGAA CTACTGGAAG 
GCCAAAGACC TCTGGCAGAA GATCGACAAG AGAGCCGCTC ATCCCGACTA TGACCAGGGA 
AAAGCATGTC ACCAAAACAA GTGTTTGGTG TTGGGCGCGG GTCCCTGTGG TCTCAGGACA 
GCCATTGAAT TGGCTCTGCT TGGCGCTCAG GTTGTTGTTC TGGAGAAAAG ATCATCTTTC 
ACGAGGAATA ATGTCCTGCA TTTGTGGCCC TTCACCATTA AAGACCTTCT CAACCTCGGG 
GCAAAGAAAT TTTATGGCCG ATTCTGCAGT GGCTCCATCC ATCACATAAG TATCCGTCAG 
CTGCAGTTGA TTCTGCTGAA AGTGGCTCTG TTTTTGGGGG TGGAGGTTCA CACTGGTGTG 
GCTTTTGAAG GTCTAAATGA ACCATCAGGA TCTGCAGGAT GGAGAGCAAA TGTGTCTCCA 
AAGTCCCACC CAGTTGCAGA TTTTCAGTTT GATGTGTTTA TTTCAGCCGG AGGGGGGAAA 
TATGTGCCTG ATGGTTTTAA GATAAAGGAG CTCCGAGGAA AGCTGGCTAT TGGTATCACT 
GCAAACTTTG TAAACCGTCA CACTAAACAA GAGGCTCAGG TGCAGGAGAT CAGCGGTGTG 
GCCCGAATTT ACAACCAGCA GTTTTTCCAG GCTCTTCAGT CTGAGATTGG CGTTGATCTA 
GAAAACATTG TCTACTACAA AGACGACACC CACTATTTTG TGATGACTGC AAAAAAGGCC 
AGCTTGTTAA AGAAAGGAGT CATAAAGCAG GATTTCTCTG ATGCCGATAA GCTCCTGGCT 
CCCTCAAATG TGAATCAGGA GGCACTGCAG GATTACGCAT TCGAAGCATG TGATTTCTCC 
ACAGAGCACT TGCTGCCCAA CCTGGAATTC GCCAAGAACC ACAAAGGCCA AGCGGACGTG 
GCCATGTTTG ACTTTACCTG CATGCAGAGG GCAGAGAGCG CCTCTCTGGT GAAGGAGAGG 
AATGGCAAGA GGCTGCTCAT CGGGCTGGTG GGAGATTCTC TGGTAGAGCC TTTTTGGCCT 
TTGGGCACTG GCATTGCTCG TGGTTTTCTG GGTGCGTTTG ATGCAGCGTG GATGGTCAGG 
AGTTGGGGAA AAGGTGTGCA GCCCATGGAG GTTCTGGCAG AGAGGGAAAG TGTGTATCAG 
TTGCTTTCTC AAACAACTCC AGAAAACACC AGCAAGAACT ACATGGCCTA CAGCATTGAT 
CCAAGCACAC GATACCCGAA CATCAACCTG TCCTCCATCA AACCCAGACA GGTCAAGCGT 
CTGTATGAAG CAGAAGAGCA GGAAAGCAAA CCAAACAAAC TGAAGAAACC TGACATAAAA 
GCTAAACCTA GAAAAGACTC TATGAAGCGT CTGGAGGAGC TGTTGTCCTG GTGTCAGAAG 
AATACAGTGG GATACGAGCA TGTGAAGGTC AAAGATCTGA GCGAGTCTTG GAGGTCTGGT 
CTGGCTCTCT GTGCTCTCAT CCACAGCTTC AGACCTGAGC TTGTTGACAT GTCGGCTCTG 
GATGAGTATA ATATCATCAA GAACAATAAG TTGGCTTTTG ATCTGATGGA GAAAGAGTTC 
GGCATCACCC CCATCATGCG GCCCGGCGAT ATGATGACCT GTGGTAAAAT CGACCAGCTG 
TCCATGGTGG TTTACCTCAC ACAGATACGC AACGCTCTTA CTGAGAAAGA CACACCAGCA 
GCGCAGTCCA ACACACTCTC CCTGTCCAGA AAGCGTTCAG CCGTTGCCTT CCTCAACACA 
CTCAAACGCA ACTCTCTGCA AAGACATAAG GATCGACTGG CATCCGTGAA AGGACCAAGA 
CAGCAAAACA TGAAGGAGAA AGAAGAGAAG AAGGATGTGA AAGAGGAAAG TCTCTCCTCA 
GAGACGAGTG CATGTGAACC CTGTTATTTC TGCAAGAAGC ATCTGTATGT GGTGGAGAGA 
GAAAGTGCAG AGGGAAAGTT CTTCCATCGC AGCTGCTTCA ACTGTTTTCA GTGCGGCTCC 
ACTCTGCGGC AGGGCGGATA CTCTTTTCAC TCTGACAATG GGAGGTTTTA CTGCGAGCTG 
CACTCACTGG CTGAGGAAGA GGAGGGTGAT GAAGGTCATG GAGGCGCACA GAATCATACT 
GAGAACGGAA GTAAGGAGGA CAAAAATGGA GAAACAACAG CGGCTTCATC CCCACCTGCG 
CATCTCTCAA TAAAGCGTAA GGGCTCGTAC AAGATCTCTG TTGACCCTGA CTTTGATGAA 
TCAACTGAGT TTCCTGCTCC AGACCAAGAT GAACCACCTG ATCTTGAGGA GTCCCATCAA 
CCTCCCAAAC CTTCAGAGCT CAGTGCGGAA AACACAAACA TGGAAAACCA ACAACACAAC 
ATCAATCCAG TTCCTGCCCC GCGTGGCTCC AGAGCTCCGC TCCCGAAACC TCGCACTGTC 
CATAATGTGG TGCACGAACC CTGTAATATT CCAGAGGAAG CAGAGCAGAT TCCTGAGGAG 
CCCAAACCCA AACCGTCCCT GCGCAAACTC CAGCAGAGCG AAGAGGAGAA GGTGGATCTG 
TTGAGCCAGG ATTCAGACTC TGAGACACGG GGCTCGTCTT CTGCCGCCTC CACCTCCTCC 
TCATCCAAAC AGCATGAAGA GGAGGGCTAC TGGAGCGGAG GCACGACGTG GGGGAAGAGC 
CATCGTGAGC AACGAAACCG CCCCTGCATA AGGAGGAAGA GTGAACCTCC GCTGCCGCTG 
ACTGGCCATT CTCAGCACGG AAAGATGCGC TCCAAGTTTT CCCCGTGGAA TCTGTCTTCA 
CCGCGGCTCC AGCAGCGATT CAGCGTTCAC AGAGTTCCTG CAGGTCAAAG TCAGCCAGAT 
CAGTATGTAT CAGAAGATGA TAATGAAGAT GATGAAGATG AGGATGAAGA GGACCTTCAG 
GCTGAACATT ATTTGGATTG CGAGGGAGCT GATTTTGAAT TTTCGGATTC AGAGAAACGC 
AACTTAAAGA GGATGAAAAC TCTGGAGCGG AAAGCAAAAA TGACTGAAAT CCAGCGTTTC 
CACAAAGCTC AGTCGATTCA GAGACGTCTG GAAGAGATCG AAGTGACCTT TAAAGAGCTG 
GAGGAGAAAG GAGTGGAGCT GGAGCGAGCT CTGAGAGGAG AGACGGGTAC AGGTGACCCT 
GAAATCATTG ATCAGTGGAT TGAACTGGTC CAGGAGAAAA ACAACCTGCT GTCAGAGGAG 
TCTGATCTCA TGGTGGCGTC TCGACAGCTG GAGCTGGAGG ACAAACAGAG CATGCTGGAG 
ATGGAGCTGC GCCGCTACAT GGAAATGGAT GATTCAGAGA AAAGCCCTGA GCAGCAAAAG 
CACGAGGCAG AGATCCTGCA GGAGATGCTG GACGTGGTAG ACATGAGGGA TTCACTGGTG 
GCCTTTCTGG AGGAGAAGAG GCTGAAGGAG GTCAATGATC AATTTAATTC AAGCCTGGAT 
GCCAAAAGAC GTTCAACCAC AGCCTCCCAG GTGCACTGGG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Identification and expression analysis of mical family genes in zebrafish.
Journal of genetics and genomics = Yi chuan xue bao37(10)685-93(2010 Oct)
Xue Y,Kuok C,Xiao A,Zhu Z,Lin S,Zhang B

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MICAL1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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