nme2b.2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	nme2b.2
Entrez Gene ID	30084
Full Name	NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 2
Synonyms	NDPK-Z2,NM23-Z2,ndpkz2,nme2b2
General protein information	Preferred Names NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 2 Names nucleoside diphosphate kinase B non-metastatic cells 2b.2, protein (NM23B) expressed in nucleoside diphosphate kinase-Z2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_130927.2	NP_571002.1	nucleoside diphosphate kinase B
NM_130927.1	NP_571002.1	nucleoside diphosphate kinase B

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NME2	NP_001069844.1
Gallus gallus (chicken)	NME2	NP_990378.1
Rattus norvegicus (Norway rat)	Nme2	NP_114021.2
Oryza sativa (rice)	Os07g0492000	NP_001059682.1
Canis lupus familiaris (dog)	NME2	NP_001019809.1
Homo sapiens (human)	NME2	NP_002503.1
Mus musculus (house mouse)	Nme2	NP_032731.1
Danio rerio (zebrafish)	nme2b.2	NP_571002.1
Xenopus tropicalis (tropical clawed frog)	nme2	NP_001005140.1

Gene Ontology
Bibliography

Related articles in PubMed

Proteomic analysis of the Rett syndrome experimental model mecp2
Cortelazzo A, Pietri T, De Felice C, Leoncini S, Guerranti R, Signorini C, Timperio AM, Zolla L, Ciccoli L, Hayek J
Journal of proteomics154128-133(2017 02)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Comprehensive and quantitative proteomic analyses of zebrafish plasma reveals conserved protein profiles between genders and between zebrafish and human.
Li C, Tan XF, Lim TK, Lin Q, Gong Z
Scientific reports624329(2016 Apr)

Zebrafish Collagen Type I: Molecular and Biochemical Characterization of the Major Structural Protein in Bone and Skin.
Gistelinck C, Gioia R, Gagliardi A, Tonelli F, Marchese L, Bianchi L, Landi C, Bini L, Huysseune A, Witten PE, Staes A, Gevaert K, De Rocker N, Menten B, Malfait F, Leikin S, Carra S, Tenni R, Rossi A, De Paepe A, Coucke P, Willaert A, Forlino A
Scientific reports621540(2016 Feb)

Nucleoside diphosphate kinase B regulates angiogenesis through modulation of vascular endothelial growth factor receptor type 2 and endothelial adherens junction proteins.
Feng Y, Gross S, Wolf NM, Butensch?n VM, Qiu Y, Devraj K, Liebner S, Kroll J, Skolnik EY, Hammes HP, Wieland T
Arteriosclerosis, thrombosis, and vascular biology34(10)2292-300(2014 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NDPKB is required for VEGF-induced angiogenesis and contributes to the correct localization of VEGF receptor type 2 and VE-cadherin at the endothelial adherens junctions.
Title: Nucleoside diphosphate kinase B regulates angiogenesis through modulation of vascular endothelial growth factor receptor type 2 and endothelial adherens junction proteins.

The expression level of NDK-Z2 was upregulated to a greater extent than those for myogenin and myostatin 1 under restricted feeding.
Title: Feeding restriction alters expression of some ATP related genes more sensitively than the RNA/DNA ratio in zebrafish, Danio rerio.

The following nme2b.2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nme2b.2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa36678	NM_130927.1 Latest version!	Danio rerio NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 2 (nme2b.2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa159683	NM_130927.2 Latest version!	Danio rerio NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 2 (nme2b.2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nme2b.2 ( NM_130927.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571002.1 Danio rerio NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 2 (nme2b.2), mRNA.

Price & Availability↑

CloneID	ODa36678
Clone ID Related Accession (Same CDS sequence)	NM_130927.1
Accession Version	NM_130927.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-09
Organism	Danio rerio(zebrafish)
Product	nucleoside diphosphate kinase B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC055548.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC055548.1, EH448740.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGGTA AAACCGAGCG CACCTTCATC GCGGTGAAGC CGGACGGTGT GCAGAGGGGG 
CTGATGGGAG AAATCATCAA GCGCTTCGAG CAGAAAGGCT TCAGACTGGT GGCCGCCAAG 
TTTGTGCAGG CCTCTGAGGA CCTGGCAAAG CAGCACTACA TCGACCTGAA GGACCAGCCC 
TTCTATGCCG GACTGGTGAA GTACACTAGC TCTGGACCAC TTCTGGCCAT GGTCTGGGAG 
GGTCTGAATG TGATTAAGAC CGGCCGTGTG ATGCTCGGAG AGACCGATCC CTTCGCCTCC 
AAACCCGGAA CCATCAGAGG AGACTTCTGC ATTGAAGTCG GCAGGAACCT GATCCACGGC 
AGTGACTCTG AGAAGAGCGC CGCTACTGAG GTCAGCCTGT GGTTCAAGCC GGAGGAGCTG 
GTGTCCTACA GGAGCTGCGC ACAGGAGTGG ATCTACGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571002.1
CDS	55..516
Translation	MAGKTERTFIAVKPDGVQRGLMGEIIKRFEQKGFRLVAAKFVQASEDLAKQHYIDLKDQPFYAGLVKYTSSGPLLAMVWEGLNVIKTGRVMLGETDPFASKPGTIRGDFCIEVGRNLIHGSDSEKSAATEVSLWFKPEELVSYRSCAQEWIYE

Target ORF information:

RefSeq Version	NM_130927.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 2 (nme2b.2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130927.1

ORF Insert Sequence:

ATGGCTGGTA AAACCGAGCG CACCTTCATC GCGGTGAAGC CGGACGGTGT GCAGAGGGGG 
CTGATGGGAG AAATCATCAA GCGCTTCGAG CAGAAAGGCT TCAGACTGGT GGCCGCCAAG 
TTTGTGCAGG CCTCTGAGGA CCTGGCAAAG CAGCACTACA TCGACCTGAA GGACCAGCCC 
TTCTATGCCG GACTGGTGAA GTACACTAGC TCTGGACCAC TTCTGGCCAT GGTCTGGGAG 
GGTCTGAATG TGATTAAGAC CGGCCGTGTG ATGCTCGGAG AGACCGATCC CTTCGCCTCC 
AAACCCGGAA CCATCAGAGG AGACTTCTGC ATTGAAGTCG GCAGGAACCT GATCCACGGC 
AGTGACTCTG AGAAGAGCGC CGCTACTGAG GTCAGCCTGT GGTTCAAGCC GGAGGAGCTG 
GTGTCCTACA GGAGCTGCGC ACAGGAGTGG ATCTACGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nme2b.2 ( NM_130927.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571002.1 Danio rerio NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 2 (nme2b.2), mRNA.

Price & Availability↑

CloneID	ODa159683
Clone ID Related Accession (Same CDS sequence)	NM_130927.2
Accession Version	NM_130927.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-26
Organism	Danio rerio(zebrafish)
Product	nucleoside diphosphate kinase B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU694252.9 and EH432412.1. On Apr 11, 2018 this sequence version replaced NM_130927.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CO958909.1, GFIL01028344.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGGTA AAACCGAGCG CACCTTCATC GCGGTGAAGC CGGACGGTGT GCAGAGGGGG 
CTGATGGGAG AAATCATCAA GCGCTTCGAG CAGAAAGGCT TCAGACTGGT GGCCGCCAAG 
TTTGTGCAGG CCTCTGAGGA CCTGGCAAAG CAGCACTACA TCGACCTGAA GGACCAGCCC 
TTCTATGCCG GACTGGTGAA GTACACTAGC TCTGGACCAC TTCTGGCCAT GGTCTGGGAG 
GGTCTGAATG TGATTAAGAC CGGCCGTGTG ATGCTCGGAG AGACCGATCC CTTCGCCTCC 
AAACCCGGAA CCATCAGAGG AGACTTCTGC ATTGAAGTCG GCAGGAACCT GATCCACGGC 
AGTGACTCTG AGAAGAGCGC CGCCACTGAG GTCAGCCTGT GGTTCAAGCC GGAGGAGCTG 
GTGTCCTACA GGAGCTGCGC ACAGGAGTGG ATCTACGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571002.1
CDS	93..554
Translation	MAGKTERTFIAVKPDGVQRGLMGEIIKRFEQKGFRLVAAKFVQASEDLAKQHYIDLKDQPFYAGLVKYTSSGPLLAMVWEGLNVIKTGRVMLGETDPFASKPGTIRGDFCIEVGRNLIHGSDSEKSAATEVSLWFKPEELVSYRSCAQEWIYE

Target ORF information:

RefSeq Version	NM_130927.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 2 (nme2b.2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130927.2

ORF Insert Sequence:

ATGGCTGGTA AAACCGAGCG CACCTTCATC GCGGTGAAGC CGGACGGTGT GCAGAGGGGG 
CTGATGGGAG AAATCATCAA GCGCTTCGAG CAGAAAGGCT TCAGACTGGT GGCCGCCAAG 
TTTGTGCAGG CCTCTGAGGA CCTGGCAAAG CAGCACTACA TCGACCTGAA GGACCAGCCC 
TTCTATGCCG GACTGGTGAA GTACACTAGC TCTGGACCAC TTCTGGCCAT GGTCTGGGAG 
GGTCTGAATG TGATTAAGAC CGGCCGTGTG ATGCTCGGAG AGACCGATCC CTTCGCCTCC 
AAACCCGGAA CCATCAGAGG AGACTTCTGC ATTGAAGTCG GCAGGAACCT GATCCACGGC 
AGTGACTCTG AGAAGAGCGC CGCCACTGAG GTCAGCCTGT GGTTCAAGCC GGAGGAGCTG 
GTGTCCTACA GGAGCTGCGC ACAGGAGTGG ATCTACGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Proteomic analysis of the Rett syndrome experimental model mecp2
Journal of proteomics154128-133(2017 02)
Cortelazzo A,Pietri T,De Felice C,Leoncini S,Guerranti R,Signorini C,Timperio AM,Zolla L,Ciccoli L,Hayek J

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Comprehensive and quantitative proteomic analyses of zebrafish plasma reveals conserved protein profiles between genders and between zebrafish and human.
Scientific reports624329(2016 Apr)
Li C,Tan XF,Lim TK,Lin Q,Gong Z

Zebrafish Collagen Type I: Molecular and Biochemical Characterization of the Major Structural Protein in Bone and Skin.
Scientific reports621540(2016 Feb)
Gistelinck C,Gioia R,Gagliardi A,Tonelli F,Marchese L,Bianchi L,Landi C,Bini L,Huysseune A,Witten PE,Staes A,Gevaert K,De Rocker N,Menten B,Malfait F,Leikin S,Carra S,Tenni R,Rossi A,De Paepe A,Coucke P,Willaert A,Forlino A

Nucleoside diphosphate kinase B regulates angiogenesis through modulation of vascular endothelial growth factor receptor type 2 and endothelial adherens junction proteins.
Arteriosclerosis, thrombosis, and vascular biology34(10)2292-300(2014 Oct)
Feng Y,Gross S,Wolf NM,Butensch?n VM,Qiu Y,Devraj K,Liebner S,Kroll J,Skolnik EY,Hammes HP,Wieland T

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nme2b.2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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