Genomic DNA Sequencing
Affordable sequencing of single and pooled clones
Starting from just $80 per clone and 2-week turnaround
Genomic DNA Sequencing
CRISPR gene edited mammalian cell lines play an important role for a variety of applications, from drug discovery, establishment of primary cell lines, to epigenetic studies. While CRISPR is considered to be one of the most efficient and accurate editing methods, off-targeting can still be an issue, and therefore verification of gene knock-in and knock-out is a critical component of CRISPR quality control. To make this process even easier, GenScript offers a genomic DNA sequencing service to ensure CRISPR specificity in modified single cell clones or pools.
Advantages of genomic DNA sequencing:
- Less expensive: while Next Generation Sequencing (NGS) may be the most comprehensive, it is expensive and unnecessary for verifying single cell clones or pools
- Less labor intensive: PCR and sanger sequencing require expertise and very labor intensive
- Shorter turnaround time compared to NGS
Service details
| Service |
Deliverables |
Technique |
Delivery |
Turnaround time |
Single clone
Genomic DNA Sequencing |
AB1 files
Alignment files |
PCR Sequencing |
1-2 effective sequencing results |
2 weeks |
| Pooled clone genomic DNA sequencing |
AB1 files
Alignment files
Mutation statistic report |
PCR, TA Cloning, plasmid prep and sequencing |
≥20 effective sequencing results |
3 weeks |
*Pricing per target, up to 800bp